##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935884.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468162
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953302489309255	33.0	31.0	34.0	30.0	34.0
2	31.99581341501446	33.0	31.0	34.0	30.0	34.0
3	32.0428420076811	33.0	31.0	34.0	30.0	34.0
4	35.74380235901248	37.0	35.0	37.0	35.0	37.0
5	33.61300789043109	37.0	35.0	37.0	32.0	37.0
6	34.60097145859766	37.0	35.0	37.0	30.0	37.0
7	35.68932976192002	37.0	35.0	37.0	32.0	37.0
8	35.68778756071616	37.0	35.0	37.0	33.0	37.0
9	37.565635399712065	39.0	37.0	39.0	35.0	39.0
10	37.13276600834754	39.0	37.0	39.0	34.0	39.0
11	37.150994741136614	39.0	37.0	39.0	34.0	39.0
12	37.075546498861506	39.0	37.0	39.0	34.0	39.0
13	36.99181907117622	39.0	37.0	39.0	33.0	39.0
14	38.0834326579261	40.0	37.0	41.0	33.0	41.0
15	38.154813931929546	40.0	37.0	41.0	33.0	41.0
16	38.22481747771071	40.0	37.0	41.0	34.0	41.0
17	38.170955780264094	40.0	37.0	41.0	34.0	41.0
18	38.16941357906024	40.0	37.0	41.0	34.0	41.0
19	38.08832839914388	40.0	37.0	41.0	34.0	41.0
20	38.05102720853038	40.0	37.0	41.0	34.0	41.0
21	38.04149418363729	40.0	37.0	41.0	34.0	41.0
22	38.09185282017763	40.0	37.0	41.0	34.0	41.0
23	38.07145603444961	40.0	37.0	41.0	34.0	41.0
24	38.022156860232144	40.0	36.0	41.0	34.0	41.0
25	37.883578761198045	40.0	36.0	41.0	33.0	41.0
26	37.90953772412114	40.0	36.0	41.0	34.0	41.0
27	37.89374618187721	40.0	36.0	41.0	34.0	41.0
28	37.82564582345427	40.0	36.0	41.0	33.0	41.0
29	37.853642542538694	40.0	36.0	41.0	33.0	41.0
30	37.711409725693244	40.0	36.0	41.0	33.0	41.0
31	37.70968382739308	40.0	36.0	41.0	33.0	41.0
32	37.62971578214379	40.0	36.0	41.0	33.0	41.0
33	37.514398861932406	40.0	36.0	41.0	33.0	41.0
34	37.403339015127244	40.0	36.0	41.0	33.0	41.0
35	37.36946826098658	40.0	36.0	41.0	33.0	41.0
36	37.25119509913235	40.0	35.0	41.0	33.0	41.0
37	37.139054429876836	40.0	35.0	41.0	32.0	41.0
38	37.06789530119916	39.0	35.0	41.0	32.0	41.0
39	37.01144262028956	39.0	35.0	41.0	32.0	41.0
40	36.90659857058027	39.0	35.0	41.0	31.0	41.0
41	36.85770737479761	39.0	35.0	41.0	31.0	41.0
42	36.850457747531834	39.0	35.0	41.0	31.0	41.0
43	36.79510511318732	39.0	35.0	41.0	31.0	41.0
44	36.635679529735434	39.0	35.0	41.0	31.0	41.0
45	36.64740410370769	39.0	35.0	41.0	31.0	41.0
46	36.59885466996467	39.0	35.0	41.0	31.0	41.0
47	36.51373883399336	39.0	35.0	41.0	31.0	41.0
48	36.48115182351408	39.0	35.0	41.0	31.0	41.0
49	36.39565791328643	39.0	35.0	40.0	31.0	41.0
50	36.26563881733246	39.0	35.0	40.0	31.0	41.0
51	35.03850162977773	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	10.0
11	7.0
12	8.0
13	3.0
14	11.0
15	13.0
16	19.0
17	48.0
18	92.0
19	221.0
20	440.0
21	806.0
22	1203.0
23	1456.0
24	1793.0
25	2403.0
26	3134.0
27	3765.0
28	4342.0
29	5088.0
30	6443.0
31	8624.0
32	11674.0
33	17251.0
34	29339.0
35	39982.0
36	36414.0
37	55059.0
38	95391.0
39	143023.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13274892024556	18.852234910137945	23.076627321311854	11.938388848304648
2	29.76320162678731	28.52922706242711	24.905908638462755	16.801662672322827
3	30.689163152925698	28.03965294064875	24.798894399801778	16.472289506623774
4	27.727795079481034	24.445811492602985	28.44699057163973	19.379402856276247
5	26.289831297713185	32.78138763932143	22.178861163443422	18.74991989952196
6	24.414412105211444	37.53615201575523	23.48802337652351	14.561412502509816
7	78.06122666940077	5.410306688710318	12.049247909911525	4.479218731977393
8	78.58049136837249	7.999154138951901	8.322119266407782	5.09823522626783
9	72.70795152105468	6.613095466953747	10.012346153681845	10.66660685830973
10	40.42724526979977	26.014712855806323	17.875863483153267	15.682178391240639
11	31.89494234901594	24.36891503368492	23.69115818883207	20.044984428467068
12	26.54294880831849	22.031689885125235	29.867866251425788	21.557495055130488
13	24.549408110867603	24.91445268945365	32.60858420803056	17.92755499164819
14	20.43886517914739	30.22692999431821	28.592666641034516	20.74153818549989
15	18.59740858933446	24.595545986218443	36.530730815401505	20.27631460904559
16	21.20377134410738	24.355244552099485	30.43497763594653	24.0060064678466
17	21.6472075905349	24.383439920369447	28.95707041579624	25.012282073299414
18	21.658314856823065	24.35289493807699	30.771826846262616	23.216963358837326
19	22.381355171927666	26.332124350118118	27.297174909539862	23.989345568414354
20	24.141216074777535	25.96665256898253	29.121970599920537	20.770160756319395
21	23.196671237733945	28.330577876888768	28.386327809604367	20.086423075772917
22	20.759480692580773	24.544281680273066	28.630687667944006	26.065549959202155
23	21.077746591991662	27.48514403133958	28.143890362737682	23.293219013931076
24	21.93514210892811	25.479000858677125	28.31968421187538	24.266172820519394
25	20.147726641632595	28.9643328591385	27.48428962624049	23.40365087298841
26	19.782254860497005	26.27381120210525	29.709801308094207	24.23413262930353
27	22.118412002682835	27.870480731029	27.17072295487459	22.84038431141357
28	18.234927226045684	26.828533712689197	31.049722104741523	23.886816956523596
29	21.44300477185248	24.625877367236125	29.878332713889638	24.05278514702176
30	20.327792516265735	28.870134696963873	28.74517795122201	22.05689483554838
31	22.905532700219155	27.148722023573036	24.92790956976431	25.0178357064435
32	26.174059406786537	27.729503889679215	24.544281680273066	21.55215502326118
33	23.36904746647528	27.8852192189883	24.584865922479825	24.160867392056595
34	22.387336007621293	26.81251361708127	27.506931361366366	23.293219013931076
35	23.33807528163328	27.08058321692064	26.0226161029729	23.55872539847318
36	24.513736697980615	27.136119548361464	26.957976085201274	21.392167668456647
37	23.363921035880743	28.72723544414113	26.980404219052378	20.928439300925746
38	23.297063836876976	27.554991648190157	24.17539227874112	24.972552236191746
39	24.844177870053528	27.30422375160735	26.384670263712135	21.466928114626988
40	23.497849035163043	26.61813645703838	27.80554594349819	22.07846856430039
41	22.580858762565096	27.23117211563519	25.566790982608584	24.621178139191137
42	24.487890943733152	24.263823206496895	27.973863748018847	23.274422101751103
43	25.55141169082497	24.162362600980003	26.85672908095916	23.42949662723587
44	22.430269863850548	25.334606396930976	27.955707639663192	24.27941609955528
45	21.49256026759968	25.223320132774553	27.14295478915418	26.14116481047159
46	26.04290822407628	26.23173175097509	26.625398900380638	21.099961124567994
47	20.909428787471004	25.402745203583375	30.9546695374678	22.733156471477823
48	22.42706584472896	25.343577650471417	27.598566308243726	24.630790196555893
49	22.42065780648579	22.28523459828008	31.873582221538697	23.42052537369543
50	22.937572891435014	23.150106159833562	29.273200302459408	24.639120646272016
51	21.541474959522557	23.394466018173198	27.15961568858643	27.904443333717815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	327.5
2	496.0
3	1085.5
4	1675.0
5	1168.0
6	661.0
7	668.5
8	676.0
9	705.5
10	735.0
11	740.0
12	745.0
13	777.5
14	810.0
15	870.0
16	930.0
17	961.0
18	992.0
19	867.5
20	743.0
21	922.0
22	1101.0
23	1357.5
24	1614.0
25	2016.5
26	2733.5
27	3048.0
28	3602.0
29	4156.0
30	5208.5
31	6261.0
32	7442.5
33	8624.0
34	9939.5
35	11255.0
36	11779.0
37	12303.0
38	13786.0
39	15269.0
40	17977.5
41	20686.0
42	23713.5
43	26741.0
44	30047.0
45	33353.0
46	45359.0
47	57365.0
48	53868.5
49	50372.0
50	49463.0
51	48554.0
52	42224.5
53	35895.0
54	32154.5
55	28414.0
56	24986.5
57	21559.0
58	20231.0
59	18903.0
60	17024.0
61	15145.0
62	13364.0
63	11583.0
64	10238.5
65	8894.0
66	7252.5
67	5611.0
68	4693.5
69	3776.0
70	3199.0
71	2622.0
72	2193.0
73	1764.0
74	1430.5
75	838.0
76	579.0
77	440.5
78	302.0
79	230.5
80	159.0
81	101.5
82	44.0
83	47.0
84	50.0
85	26.5
86	3.0
87	4.5
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	2.0
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.62530884730656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8109411995175	23.026685028978182
2	10.766570530103921	6.809922364816913
3	3.82815227977421	3.631994944871403
4	1.829854900686455	2.3147890551986645
5	1.1758653739031377	1.8593552806270168
6	0.8022641912677162	1.5223111695585687
7	0.6076088310114347	1.3451071857361265
8	0.5342511368386657	1.3516685763637954
9	0.48852955216912186	1.3904908171546357
>10	7.011488182741611	47.88762000127389
>50	0.10038556645053846	2.056063158299646
>100	0.0359488852829631	1.8537817229030296
>500	0.004069685126373181	0.9968206047593587
>1k	0.0033914042719776507	2.8194986074794515
>5k	6.782808543955301E-4	1.133891481979335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	5286	1.129096338446948	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	4414	0.9428360268454082	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	4133	0.8828140686343615	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	2447	0.522682319368082	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	1130	0.24136944049282086	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT	1020	0.217873300267856	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	862	0.18412429885381554	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT	847	0.18092027973222943	No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	792	0.169172209619747	Illumina Single End Adapter 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	734	0.15678333568294736	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC	733	0.15656973440817495	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA	679	0.14503526557046492	No Hit
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	475	0.10146060551689373	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1360127477240785E-4	0.0	0.0	0.179638672083595	0.0
2	2.1360127477240785E-4	0.0	0.0	1.1636997449600779	0.0
3	2.1360127477240785E-4	0.0	0.0	1.500548955276165	0.0
4	2.1360127477240785E-4	0.0	0.0	2.1005549361118585	0.0
5	2.1360127477240785E-4	0.0	0.0	4.208585916840752	0.0
6	2.1360127477240785E-4	0.0	0.0	5.0698262567231005	0.0
7	2.1360127477240785E-4	0.0	0.0	6.0669170073607	0.0
8	2.1360127477240785E-4	0.0	0.0	7.491210307543115	0.0
9	2.1360127477240785E-4	0.0	0.0	7.954938675074013	0.0
10	2.1360127477240785E-4	0.0	0.0	9.99504445042528	0.0
11	2.1360127477240785E-4	0.0	0.0	11.383879938995475	0.0
12	2.1360127477240785E-4	0.0	0.0	13.716619460784942	0.0
13	2.1360127477240785E-4	0.0	0.0	14.205125576189438	0.0
14	2.1360127477240785E-4	0.0	0.0	14.433038136371598	0.0
15	4.272025495448157E-4	0.0	0.0	14.913854605884287	0.0
16	4.272025495448157E-4	0.0	0.0	15.696916879199936	0.0
17	4.272025495448157E-4	0.0	0.0	16.57994454910907	0.0
18	4.272025495448157E-4	0.0	0.0	17.514236524963582	0.0
19	4.272025495448157E-4	0.0	0.0	18.297085197004456	0.0
20	4.272025495448157E-4	0.0	0.0	18.880003075858358	0.0
21	4.272025495448157E-4	0.0	0.0	19.515894070855815	0.0
22	4.272025495448157E-4	0.0	0.0	20.20219496669956	0.0
23	4.272025495448157E-4	0.0	0.0	20.854319658579723	0.0
24	4.272025495448157E-4	0.0	0.0	21.39259487100619	0.0
25	4.272025495448157E-4	0.0	0.0	21.863158479329805	0.0
26	4.272025495448157E-4	0.0	0.0	22.308730738505048	0.0
27	6.408038243172235E-4	0.0	0.0	22.779507948103433	0.0
28	6.408038243172235E-4	0.0	0.0	23.21824496648596	0.0
29	6.408038243172235E-4	0.0	0.0	23.679837321269133	0.0
30	6.408038243172235E-4	0.0	0.0	24.24096787009625	0.0
31	6.408038243172235E-4	0.0	0.0	24.706405047825324	0.0
32	6.408038243172235E-4	0.0	0.0	25.185512707139836	0.0
33	6.408038243172235E-4	0.0	0.0	25.64710506192301	0.0
34	6.408038243172235E-4	0.0	0.0	26.077511630589413	0.0
35	8.544050990896314E-4	0.0	0.0	26.567512954917316	0.0
36	8.544050990896314E-4	0.0	0.0	27.021415663808682	0.0
37	8.544050990896314E-4	0.0	0.0	27.467628726808243	0.0
38	8.544050990896314E-4	0.0	0.0	27.906152143915996	0.0
39	8.544050990896314E-4	0.0	0.0	28.367530897424395	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.028997E-4	45.000004	1
GCGAACC	20	7.028997E-4	45.000004	33
TAGCGCG	30	2.1627347E-6	45.000004	1
GTTAGCG	35	1.2098644E-7	45.000004	1
CCTCATG	20	7.028997E-4	45.000004	34
ATAACGG	30	2.1627347E-6	45.000004	2
GCGTCGC	20	7.028997E-4	45.000004	3
TCGACAA	35	1.2098644E-7	45.000004	19
GCATCCG	20	7.028997E-4	45.000004	1
TCGTTCA	25	3.887251E-5	45.0	16
GTTACGT	25	3.887251E-5	45.0	23
CGCACGG	25	3.887251E-5	45.0	2
TAGTCGT	25	3.887251E-5	45.0	15
TTACGGG	125	0.0	44.999996	3
TACGGGC	70	0.0	41.785717	4
GGCGTGT	65	0.0	41.53846	8
CGGCGAA	60	3.6379788E-12	41.250004	31
TACGGCT	640	0.0	41.132816	7
CGGGAAC	105	0.0	40.714287	6
ATGGTTA	45	1.924127E-8	40.000004	5
>>END_MODULE