##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935883.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629468
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858451899063972	33.0	31.0	34.0	30.0	34.0
2	31.910060559075283	33.0	31.0	34.0	30.0	34.0
3	31.958458571365025	33.0	31.0	34.0	30.0	34.0
4	35.66399245076795	37.0	35.0	37.0	33.0	37.0
5	33.6004435491558	37.0	35.0	37.0	32.0	37.0
6	34.55794099144039	37.0	35.0	37.0	30.0	37.0
7	35.642633144178895	37.0	35.0	37.0	32.0	37.0
8	35.61014062668794	37.0	35.0	37.0	33.0	37.0
9	37.501461551659496	39.0	37.0	39.0	35.0	39.0
10	37.05601079006399	39.0	37.0	39.0	33.0	39.0
11	37.023653307237225	39.0	37.0	39.0	33.0	39.0
12	37.00948419935565	39.0	37.0	39.0	33.0	39.0
13	36.93817954208951	39.0	37.0	39.0	33.0	39.0
14	38.04859341539205	40.0	37.0	41.0	33.0	41.0
15	38.0874786327502	40.0	37.0	41.0	33.0	41.0
16	38.18424606175373	40.0	37.0	41.0	34.0	41.0
17	38.08142431386504	40.0	37.0	41.0	33.0	41.0
18	38.10336982976101	40.0	37.0	41.0	33.0	41.0
19	38.02439202628251	40.0	37.0	41.0	34.0	41.0
20	37.96683866376051	40.0	37.0	41.0	33.0	41.0
21	38.01093939644271	40.0	37.0	41.0	34.0	41.0
22	38.08016134259406	40.0	37.0	41.0	34.0	41.0
23	38.06026199902139	40.0	37.0	41.0	34.0	41.0
24	38.003155045212786	40.0	37.0	41.0	33.0	41.0
25	37.87429067085221	40.0	36.0	41.0	33.0	41.0
26	37.89059968100047	40.0	36.0	41.0	33.0	41.0
27	37.84489918470835	40.0	36.0	41.0	33.0	41.0
28	37.76508734359808	40.0	36.0	41.0	33.0	41.0
29	37.79291401628041	40.0	36.0	41.0	33.0	41.0
30	37.665479420717176	40.0	36.0	41.0	33.0	41.0
31	37.66231166635953	40.0	36.0	41.0	33.0	41.0
32	37.566718244612915	40.0	36.0	41.0	33.0	41.0
33	37.48081395718289	40.0	36.0	41.0	33.0	41.0
34	37.41475023353054	40.0	36.0	41.0	33.0	41.0
35	37.32015765694205	40.0	36.0	41.0	33.0	41.0
36	37.229414362604615	40.0	36.0	41.0	32.0	41.0
37	37.16455006449891	40.0	36.0	41.0	32.0	41.0
38	37.09134856736164	39.0	35.0	41.0	32.0	41.0
39	37.05789968671958	39.0	35.0	41.0	32.0	41.0
40	36.957530803789865	39.0	35.0	41.0	31.0	41.0
41	36.850572229247554	39.0	35.0	41.0	31.0	41.0
42	36.891441661847786	39.0	35.0	41.0	31.0	41.0
43	36.80254595944512	39.0	35.0	41.0	31.0	41.0
44	36.691625308991085	39.0	35.0	41.0	31.0	41.0
45	36.69605126869039	39.0	35.0	41.0	31.0	41.0
46	36.59971118468294	39.0	35.0	41.0	31.0	41.0
47	36.45388010192734	39.0	35.0	41.0	31.0	41.0
48	36.384500880108284	39.0	35.0	41.0	30.0	41.0
49	36.35776083931193	39.0	35.0	40.0	30.0	41.0
50	36.20305718479732	39.0	35.0	40.0	30.0	41.0
51	34.951697306296744	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	7.0
10	8.0
11	5.0
12	11.0
13	11.0
14	15.0
15	11.0
16	37.0
17	47.0
18	110.0
19	301.0
20	712.0
21	1225.0
22	1929.0
23	2338.0
24	2801.0
25	3685.0
26	4363.0
27	5039.0
28	5969.0
29	7271.0
30	9207.0
31	12149.0
32	16383.0
33	23069.0
34	38238.0
35	50299.0
36	48637.0
37	72898.0
38	126918.0
39	195675.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.618573144305984	18.430643019184455	22.935876009582696	12.014907826926866
2	29.28743002027109	29.50507412608743	24.580280490827175	16.62721536281431
3	29.269478353149008	29.5551163839941	25.62179491252931	15.55361035032758
4	26.437404284252736	24.662889932450895	31.492943247313605	17.406762535982768
5	27.251742741489636	32.004962921069854	22.820540519931114	17.92275381750939
6	23.84823374659236	37.93838606569357	23.919087229215783	14.294292958498286
7	76.74290035394968	4.987703902342931	13.743986985835658	4.525408757871727
8	77.17977720869051	9.165835276773402	8.393278133280802	5.261109381255282
9	72.15537565054936	6.114369594641825	9.759987799220928	11.970266955587894
10	39.53386033920708	28.182687602864643	17.50494068006634	14.77851137786194
11	29.0286400579537	24.15500073077583	26.746077640165982	20.070281571104488
12	24.15690710250561	20.857136502570427	31.021116244193507	23.964840150730456
13	22.952239033596626	23.53161717513837	34.74171840347722	18.774425387787783
14	20.002287646075732	29.77911506224304	28.801940686420913	21.416656605260314
15	17.45013249283522	25.178722349666703	37.094816575266734	20.276328582231347
16	19.862010459626227	25.31725202869725	29.41642784065274	25.404309671023782
17	20.082355258726416	26.091556679608814	30.60822790038572	23.21786016127905
18	20.591356510577185	25.063863452947565	31.028106273869362	23.316673762605884
19	20.517325741737466	27.0372759218896	28.573176078847535	23.8722222575254
20	22.337434150743167	26.91844541740009	31.098324299249526	19.645796132607217
21	21.40347086746268	29.835670756893123	29.05183424733267	19.709024128311526
22	20.223585631040816	23.803434010942574	30.483837145017695	25.489143212998915
23	19.848824721828592	28.145354489823156	28.996231738547472	23.009589049800784
24	20.520026435021318	25.60400210971805	29.040554881264814	24.83541657399582
25	18.704048498096807	31.234153284996218	27.420297775264192	22.641500441642783
26	20.12508975833561	26.119516798312226	29.56528369988625	24.19010974346591
27	21.52230137195219	28.539337980643975	27.120838549378206	22.817522098025634
28	17.934986369442132	26.452496393780145	32.92828229552575	22.684234941251976
29	22.101361784872307	24.08414724815241	30.128775410346513	23.68571555662877
30	20.064880184536786	29.89206758723239	29.546378846899284	20.496673381331536
31	22.35427376768954	25.451174642714165	27.524989356091172	24.66956223350512
32	22.895842203257356	28.231617810595615	27.502112895333834	21.370427090813195
33	21.09289113981966	27.535474400604954	26.23405796640973	25.13757649316566
34	22.57938449611418	24.367878907267723	28.127085094079447	24.925651502538653
35	20.345275693124986	26.30093984126278	27.19534591115037	26.15843855446186
36	24.917867151308723	26.193229838530314	27.19232748924489	21.696575520916074
37	19.38716503460065	27.234108802989194	31.629884283236002	21.74884187917416
38	19.26404519371914	27.841923656166795	28.471661784236847	24.422369365877216
39	21.72199381064645	25.055284780163568	29.426595156544895	23.79612625264509
40	20.136686853025093	26.23437569503136	28.126767365457816	25.50217008648573
41	21.54883171185827	25.203346317843007	25.27404093615561	27.97378103414312
42	21.392509230016458	23.72400185553515	29.499672739519724	25.38381617492867
43	24.831762694847075	24.172475804965462	27.609822898066305	23.385938602121158
44	21.223001010377015	25.07641373350194	29.31348376724472	24.38710148887632
45	19.98147642135899	23.664268874668767	28.345364657139044	28.0088900468332
46	24.737556158533874	25.64927843830028	27.284786518139125	22.32837888502672
47	19.17825846587912	24.429677124174702	33.152598702396304	23.23946570754987
48	21.111795992806623	25.18809534400478	27.93835429283141	25.761754370357192
49	20.567050271022513	22.06418753614163	33.20184663874891	24.16691555408694
50	21.395368787611126	22.592411369600995	30.41917937051605	25.593040472271824
51	20.528128514872876	22.320276805175162	27.63810074539135	29.513493934560614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	396.0
2	624.0
3	1487.0
4	2350.0
5	1623.0
6	896.0
7	905.0
8	914.0
9	946.5
10	979.0
11	1005.5
12	1032.0
13	1019.5
14	1007.0
15	1035.5
16	1064.0
17	1150.5
18	1237.0
19	1238.5
20	1240.0
21	1308.0
22	1376.0
23	1768.5
24	2161.0
25	2661.5
26	4056.5
27	4951.0
28	5927.0
29	6903.0
30	8407.5
31	9912.0
32	11385.5
33	12859.0
34	14400.5
35	15942.0
36	17402.0
37	18862.0
38	21592.0
39	24322.0
40	26382.5
41	28443.0
42	32762.0
43	37081.0
44	42609.0
45	48137.0
46	69747.5
47	91358.0
48	80243.5
49	69129.0
50	67381.5
51	65634.0
52	55540.0
53	45446.0
54	39856.0
55	34266.0
56	29898.5
57	25531.0
58	23439.5
59	21348.0
60	18490.5
61	15633.0
62	14030.0
63	12427.0
64	10551.5
65	8676.0
66	7012.0
67	5348.0
68	4344.5
69	3341.0
70	2844.5
71	2348.0
72	1955.0
73	1562.0
74	1206.5
75	706.5
76	562.0
77	374.0
78	186.0
79	153.0
80	120.0
81	81.0
82	42.0
83	29.5
84	17.0
85	13.0
86	9.0
87	6.5
88	4.0
89	2.5
90	1.0
91	3.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.80252814629719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92796323647795	22.874910753339194
2	11.43502233540078	7.273252393502407
3	4.090505935179543	3.902652904084294
4	1.9897753486206113	2.531195461172612
5	1.2229446544888727	1.944636589787303
6	0.848969155581182	1.6199619279425221
7	0.6955579523769693	1.5484350950494585
8	0.5575870942992094	1.4186143408370138
9	0.4785483864028728	1.3697143675148218
>10	6.620150464215062	44.58102708835794
>50	0.09233011471731012	1.9106897785661376
>100	0.03311841071374471	1.9095726960896797
>500	0.0015053823051702144	0.26841895995510956
>1k	0.004516146915510643	2.4622093538331664
>5k	0.0010035882034468094	2.6947220914399406
>10k+	5.017941017234047E-4	1.6899861985283058
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	10590	1.682373051529228	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	8992	1.4285078828471025	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	7894	1.2540748695724009	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3595	0.5711171973793744	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	1964	0.3120095064403592	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTACCGT	1657	0.2632381630202012	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	1534	0.24369785278997502	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	1495	0.23750214466819597	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTC	1420	0.22558732135708248	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	1387	0.22034479910019253	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1231	0.19556196661307643	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	1146	0.18205850019381445	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19730947403203974	0.0
2	0.0	0.0	0.0	1.2378707098692865	0.0
3	0.0	0.0	0.0	1.5873721936619494	0.0
4	0.0	0.0	0.0	2.277478759841644	0.0
5	0.0	0.0	0.0	4.707943850997985	0.0
6	0.0	0.0	0.0	5.587416675668978	0.0
7	0.0	0.0	0.0	6.682786098737346	0.0
8	1.5886431081484683E-4	0.0	0.0	8.171503555383277	0.0
9	1.5886431081484683E-4	0.0	0.0	8.62823844897596	0.0
10	1.5886431081484683E-4	0.0	0.0	11.166890135797212	0.0
11	1.5886431081484683E-4	0.0	0.0	12.620021986820618	0.0
12	1.5886431081484683E-4	0.0	0.0	15.664497639276341	0.0
13	1.5886431081484683E-4	0.0	0.0	16.229736857155565	0.0
14	1.5886431081484683E-4	0.0	0.0	16.46580922302643	0.0
15	1.5886431081484683E-4	0.0	0.0	17.037720741959877	0.0
16	1.5886431081484683E-4	0.0	0.0	17.81329630735796	0.0
17	1.5886431081484683E-4	0.0	0.0	18.777761538314895	0.0
18	3.1772862162969366E-4	0.0	0.0	19.757160014488424	0.0
19	3.1772862162969366E-4	0.0	0.0	20.673965952200906	0.0
20	3.1772862162969366E-4	0.0	0.0	21.31752527531185	0.0
21	3.1772862162969366E-4	0.0	0.0	22.004136826653617	0.0
22	3.1772862162969366E-4	0.0	0.0	22.84294038775601	0.0
23	3.1772862162969366E-4	0.0	0.0	23.622805289546093	0.0
24	3.1772862162969366E-4	0.0	0.0	24.259692311602812	0.0
25	3.1772862162969366E-4	0.0	0.0	24.793635260251513	0.0
26	3.1772862162969366E-4	0.0	0.0	25.273882071844795	0.0
27	3.1772862162969366E-4	0.0	0.0	25.83483195333202	0.0
28	3.1772862162969366E-4	0.0	0.0	26.343356612250346	0.0
29	3.1772862162969366E-4	0.0	0.0	26.887149148169566	0.0
30	3.1772862162969366E-4	0.0	0.0	27.492739900995762	0.0
31	3.1772862162969366E-4	0.0	0.0	28.033355150698686	0.0
32	3.1772862162969366E-4	0.0	0.0	28.553476904306493	0.0
33	4.765929324445405E-4	0.0	0.0	29.043414438859482	0.0
34	4.765929324445405E-4	0.0	0.0	29.560200041940178	0.0
35	4.765929324445405E-4	0.0	0.0	30.08302248883184	0.0
36	6.354572432593873E-4	0.0	0.0	30.581379831858012	0.0
37	6.354572432593873E-4	0.0	0.0	31.07227055227589	0.0
38	7.943215540742342E-4	0.0	0.0	31.550928720761025	0.0
39	9.53185864889081E-4	0.0	0.0	32.05405199311164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	120	0.0	45.000004	4
TCTAGCG	30	2.1639316E-6	45.000004	1
CGAATAT	30	2.1639316E-6	45.000004	14
TAGTACG	30	2.1639316E-6	45.000004	1
ACGTTAG	25	3.8887883E-5	45.0	1
TAGGACG	20	7.030853E-4	45.0	1
TCGACGG	55	1.8189894E-12	45.0	2
ATCGACG	25	3.8887883E-5	45.0	1
ACGGTAG	20	7.030853E-4	45.0	1
TCACGTA	25	3.8887883E-5	45.0	13
CGTATGG	95	0.0	42.63158	2
TACGGCT	1245	0.0	42.108433	7
CGACGGT	60	3.6379788E-12	41.250004	28
GCGATAA	60	3.6379788E-12	41.250004	9
CCGATGA	410	0.0	40.60976	18
ACGGCTG	1300	0.0	40.326923	8
CGTTAGG	140	0.0	40.178574	2
GCGTATG	90	0.0	40.0	1
GCGTTAG	85	0.0	39.705883	1
ATAGCGG	80	0.0	39.375	2
>>END_MODULE