##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638733
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980786964193175	33.0	31.0	34.0	30.0	34.0
2	32.018829463954425	33.0	31.0	34.0	30.0	34.0
3	32.06145290755292	33.0	31.0	34.0	30.0	34.0
4	35.74170897699039	37.0	35.0	37.0	33.0	37.0
5	33.73536829943028	37.0	35.0	37.0	32.0	37.0
6	34.67386059589844	37.0	35.0	37.0	31.0	37.0
7	35.741442825092804	37.0	35.0	37.0	33.0	37.0
8	35.76335495426101	37.0	35.0	37.0	35.0	37.0
9	37.631575321769816	39.0	37.0	39.0	35.0	39.0
10	37.23155841329632	39.0	37.0	39.0	34.0	39.0
11	37.20607045510409	39.0	37.0	39.0	34.0	39.0
12	37.08971041107944	39.0	37.0	39.0	34.0	39.0
13	36.99952405778314	39.0	37.0	39.0	33.0	39.0
14	37.911398033294034	40.0	37.0	41.0	33.0	41.0
15	38.054899308474745	40.0	37.0	41.0	33.0	41.0
16	38.102232075061096	40.0	37.0	41.0	33.0	41.0
17	38.13081052646411	40.0	37.0	41.0	33.0	41.0
18	38.10756294100978	40.0	37.0	41.0	34.0	41.0
19	38.03677123305043	40.0	37.0	41.0	34.0	41.0
20	38.098407315732864	40.0	37.0	41.0	34.0	41.0
21	37.9878869574611	40.0	36.0	41.0	33.0	41.0
22	38.0970358506606	40.0	36.0	41.0	34.0	41.0
23	38.114193254458435	40.0	36.0	41.0	34.0	41.0
24	38.07385245478157	40.0	36.0	41.0	34.0	41.0
25	37.8835413232133	40.0	36.0	41.0	34.0	41.0
26	37.94913210997397	40.0	36.0	41.0	34.0	41.0
27	37.92875270261596	40.0	36.0	41.0	34.0	41.0
28	37.84898384771102	40.0	36.0	41.0	34.0	41.0
29	37.865931461189575	40.0	36.0	41.0	34.0	41.0
30	37.716618681045134	40.0	36.0	41.0	33.0	41.0
31	37.65185296516698	40.0	36.0	41.0	33.0	41.0
32	37.607998960442	40.0	36.0	41.0	33.0	41.0
33	37.54411154582588	40.0	35.0	41.0	33.0	41.0
34	37.43066508228008	40.0	35.0	41.0	33.0	41.0
35	37.37352853226622	40.0	35.0	41.0	33.0	41.0
36	37.256288621380136	40.0	35.0	41.0	32.0	41.0
37	37.19333273840556	39.0	35.0	41.0	32.0	41.0
38	37.053015892399486	39.0	35.0	41.0	32.0	41.0
39	37.021340685388104	39.0	35.0	41.0	32.0	41.0
40	36.90051242068282	39.0	35.0	41.0	31.0	41.0
41	36.76244690661043	39.0	35.0	41.0	31.0	41.0
42	36.7859856935527	39.0	35.0	41.0	31.0	41.0
43	36.73686657805374	39.0	35.0	41.0	31.0	41.0
44	36.62734507219761	39.0	35.0	41.0	31.0	41.0
45	36.55867944822015	39.0	35.0	41.0	31.0	41.0
46	36.46655018607149	38.0	35.0	40.0	31.0	41.0
47	36.36782035686273	38.0	35.0	40.0	31.0	41.0
48	36.2809264590995	38.0	35.0	40.0	31.0	41.0
49	36.206749925242626	38.0	35.0	40.0	31.0	41.0
50	36.09233435566974	38.0	35.0	40.0	30.0	41.0
51	34.83409343184085	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	8.0
10	8.0
11	13.0
12	3.0
13	17.0
14	22.0
15	31.0
16	46.0
17	83.0
18	158.0
19	251.0
20	453.0
21	762.0
22	1125.0
23	1663.0
24	2290.0
25	3257.0
26	4091.0
27	5223.0
28	6088.0
29	7496.0
30	9200.0
31	12183.0
32	17001.0
33	25062.0
34	43373.0
35	51052.0
36	50748.0
37	77035.0
38	128352.0
39	191522.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32942559723703	19.371787585736136	22.269085830855772	13.029700986171061
2	31.732821069210456	24.629070362733724	24.86641523140342	18.771693336652405
3	32.9910933050273	24.91729721182403	25.21836197597431	16.873247507174362
4	29.08586216776024	25.1129971365187	25.735322897047748	20.06581779867331
5	24.828684285922286	33.35681732429669	23.249933853425453	18.564564536355565
6	25.1374204871206	34.54056702878981	24.565820147072408	15.756192337017188
7	79.71030148747599	5.678585574880271	9.595089027809742	5.016023909834
8	79.59397745223747	5.603123683918007	8.815577087765938	5.987321776078581
9	73.78857832615506	7.033768413405915	11.468954946746136	7.708698313692889
10	41.44313821268042	25.445373888620125	17.83014185896141	15.281346039738045
11	31.559509215900857	24.328788398282224	24.160329903105055	19.951372482711868
12	28.188930272899633	21.682612296530788	29.64399835298943	20.484459077580148
13	25.835051578672154	24.422098122376642	30.89005891350533	18.852791385445876
14	21.399238805572907	27.084556457862675	30.57646935417459	20.939735382389824
15	19.636217323983573	23.93879758835069	35.31084193238803	21.114143155277716
16	23.765329175101332	25.0270457295928	29.450646827391104	21.75697826791476
17	24.42335060189469	23.97245797539817	27.70265510001832	23.901536322688823
18	25.72201530216851	22.729372053737634	30.16221175358092	21.386400890512938
19	24.77936790489923	25.34799360609206	26.589044248535775	23.283594240472937
20	25.12865313049428	26.9514805090703	27.231096561474043	20.68876979896138
21	24.003926523289074	26.490254926549905	29.071458653302713	20.43435989685831
22	22.797632187471134	23.729163829017757	28.756146934634657	24.71705704887645
23	21.301545403165328	26.42935310998492	29.204221482215576	23.064880004634176
24	22.394177222720604	24.65678147207049	29.08727120721804	23.861770097990867
25	22.67003583657021	26.667793898232905	27.023811201237454	23.63835906395943
26	22.048805995619453	25.15589456001177	27.896945985255183	24.89835345911359
27	22.819237459157428	25.486862272655397	28.111746222600054	23.582154045587124
28	19.576254867057127	26.35827489733582	28.459465848797542	25.60600438680951
29	22.834736893193245	25.502674826570725	28.297739431029868	23.36484884920616
30	25.959829850657474	23.734017187150187	28.072293117781612	22.23385984441073
31	25.684754036506646	26.22864326721807	25.758337208191843	22.32826548808344
32	26.5596109798617	26.508885559380836	25.733131057891168	21.1983724028663
33	27.015200404550882	25.682249077470555	24.325500639547354	22.977049878431206
34	23.948504304615543	25.493750910004646	29.002572279810185	21.55517250556962
35	25.075109631097813	26.90936588527601	26.65965278136561	21.35587170226057
36	26.812298722627453	28.32341526114981	24.982269586822664	19.88201642940008
37	25.04583292236349	28.262356884645072	25.550269048256467	21.14154114473497
38	24.584763899782853	29.512488003594616	24.029289233529504	21.873458863093028
39	26.0983853973413	25.124269452181114	25.326231774466013	23.451113376011573
40	27.072814462380997	24.21371684256176	27.28933685906318	21.42413183599407
41	22.296640380252782	26.676091575039962	26.018070148246608	25.00919789646065
42	23.563679972695947	26.085547482281328	28.09828206778106	22.252490477241665
43	24.044945227505078	24.9655176732688	27.747587802728212	23.24194929649791
44	24.149996947081178	24.34209599316146	27.550948518395007	23.956958541362354
45	24.226867877501242	23.269660405834674	26.427944070527122	26.075527646136965
46	25.228381812118677	24.244402590753882	27.242368877136457	23.28484671999098
47	21.830404879660204	26.104960914811038	29.728071040638266	22.336563164890492
48	22.71825629801498	26.416828314804462	27.44198280032502	23.42293258685554
49	24.606995411228166	23.98748772961472	28.998345161436784	22.40717169772033
50	22.930551576323754	24.819290689536942	28.312456065366902	23.937701668772398
51	22.55699956006657	23.782707328414222	25.907695390718814	27.7525977208004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	618.0
2	985.0
3	1583.5
4	2182.0
5	1569.0
6	956.0
7	950.5
8	945.0
9	992.5
10	1040.0
11	1096.0
12	1152.0
13	1095.0
14	1038.0
15	1010.0
16	982.0
17	983.0
18	984.0
19	957.5
20	931.0
21	1220.0
22	1509.0
23	1752.0
24	1995.0
25	2711.0
26	3824.5
27	4222.0
28	4997.5
29	5773.0
30	6528.0
31	7283.0
32	8215.0
33	9147.0
34	11314.0
35	13481.0
36	14459.5
37	15438.0
38	17897.5
39	20357.0
40	22148.5
41	23940.0
42	27865.5
43	31791.0
44	36000.5
45	40210.0
46	49868.0
47	59526.0
48	63524.5
49	67523.0
50	68918.5
51	70314.0
52	62671.5
53	55029.0
54	49001.5
55	42974.0
56	38959.5
57	34945.0
58	32293.5
59	29642.0
60	26733.0
61	23824.0
62	21666.0
63	19508.0
64	17328.0
65	15148.0
66	12862.5
67	10577.0
68	8690.0
69	6803.0
70	5651.5
71	4500.0
72	3974.0
73	3448.0
74	2833.5
75	1838.0
76	1457.0
77	1073.0
78	689.0
79	486.5
80	284.0
81	240.5
82	197.0
83	124.0
84	51.0
85	38.0
86	25.0
87	18.0
88	11.0
89	9.0
90	7.0
91	5.5
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.53113046736639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13688244666162	23.141632039140546
2	11.888131443090346	7.734687099767452
3	4.352735707628696	4.247982395845004
4	2.2211206502911547	2.890222626335329
5	1.2670407297485236	2.060913364345817
6	0.9131992811054106	1.7824442973807184
7	0.667934755406099	1.5210070870262984
8	0.540111627506913	1.4056353457095185
9	0.47154032286308	1.3805765787314632
>10	6.335826958631978	45.88284811037678
>50	0.15254312981361112	3.052893808483841
>100	0.048602069750078025	2.72055386608932
>500	9.624172227738223E-4	0.1878511214285738
>1k	0.0033684602797083778	1.990752259339311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2277	0.35648698282380903	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2272	0.3557041831250303	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	2083	0.3261143545111964	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	2046	0.3203216367402342	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	1837	0.2876006093312855	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	1109	0.1736249731891103	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.17112001415301856	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	687	0.10755667861219007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.131198795114704E-4	0.0	0.0	0.1823923298154315	0.0
2	3.131198795114704E-4	0.0	0.0	1.094353978892589	0.0
3	3.131198795114704E-4	0.0	0.0	1.449588482198352	0.0
4	3.131198795114704E-4	0.0	0.0	1.971089641524706	0.0
5	3.131198795114704E-4	0.0	0.0	3.5687838267319836	0.0
6	3.131198795114704E-4	0.0	0.0	4.544152251410214	0.0
7	3.131198795114704E-4	0.0	0.0	5.520616595666734	0.0
8	3.131198795114704E-4	0.0	0.0	7.091382471236025	0.0
9	4.6967981926720554E-4	0.0	0.0	7.7090114335724005	0.0
10	4.6967981926720554E-4	0.0	0.0	9.16564511305976	0.0
11	4.6967981926720554E-4	0.0	0.0	10.687877407304773	0.0
12	4.6967981926720554E-4	0.0	0.0	12.265375360283562	0.0
13	4.6967981926720554E-4	0.0	0.0	12.828834583464452	0.0
14	4.6967981926720554E-4	0.0	0.0	13.07416400906169	0.0
15	4.6967981926720554E-4	0.0	0.0	13.499067685558755	0.0
16	4.6967981926720554E-4	0.0	0.0	14.278423065662805	0.0
17	4.6967981926720554E-4	0.0	0.0	15.185531356607534	0.0
18	4.6967981926720554E-4	0.0	0.0	16.18266161291181	0.0
19	4.6967981926720554E-4	0.0	0.0	16.811719450850354	0.0
20	4.6967981926720554E-4	0.0	0.0	17.42058105656041	0.0
21	4.6967981926720554E-4	0.0	0.0	18.147175736966776	0.0
22	7.827996987786759E-4	0.0	0.0	18.924808957733514	0.0
23	7.827996987786759E-4	0.0	0.0	19.622753169164582	0.0
24	7.827996987786759E-4	0.0	0.0	20.226448296862696	0.0
25	7.827996987786759E-4	0.0	0.0	20.750610975164896	0.0
26	7.827996987786759E-4	0.0	0.0	21.275086773346608	0.0
27	7.827996987786759E-4	0.0	0.0	21.853888870623564	0.0
28	7.827996987786759E-4	0.0	0.0	22.366622673323594	0.0
29	7.827996987786759E-4	0.0	0.0	22.926167898010593	0.0
30	7.827996987786759E-4	0.0	0.0	23.62520802901995	0.0
31	7.827996987786759E-4	0.0	0.0	24.17301125822527	0.0
32	7.827996987786759E-4	0.0	0.0	24.6938861777926	0.0
33	7.827996987786759E-4	0.0	0.0	25.22243253440796	0.0
34	7.827996987786759E-4	0.0	0.0	25.782917118733494	0.0
35	9.393596385344111E-4	0.0	0.0	26.37079969251628	0.0
36	9.393596385344111E-4	0.0	0.0	26.87413989883097	0.0
37	0.0010959195782901463	0.0	0.0	27.4183422494219	0.0
38	0.0012524795180458815	0.0	0.0	27.985089231337664	0.0
39	0.0012524795180458815	0.0	0.0	28.867930731620255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	20	7.0309296E-4	45.0	30
CAACGAT	20	7.0309296E-4	45.0	13
TCCGAAC	25	3.8888516E-5	45.0	17
GCCGATA	40	6.8066583E-9	45.0	9
CTACGCG	20	7.0309296E-4	45.0	1
AACGATT	25	3.8888516E-5	45.0	14
CCGACTC	20	7.0309296E-4	45.0	35
CGCAACA	20	7.0309296E-4	45.0	26
GTCGGTC	20	7.0309296E-4	45.0	31
ACTACGG	30	2.1639808E-6	44.999996	2
ACGGGTC	70	0.0	41.785713	5
CGTTTTT	665	0.0	40.263157	1
TACACGG	45	1.925946E-8	40.000004	2
CGTTAGG	85	0.0	39.705883	2
CTACGGG	125	0.0	39.6	3
AGCGACT	285	0.0	39.473686	19
ACGGGAC	160	0.0	39.375	5
ACAACGA	300	0.0	39.000004	13
CAACGAG	300	0.0	39.000004	14
CACACCG	75	0.0	39.000004	17
>>END_MODULE