##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693606
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879445967883782	33.0	31.0	34.0	30.0	34.0
2	31.932895620856797	33.0	31.0	34.0	30.0	34.0
3	31.9636407412854	33.0	31.0	34.0	30.0	34.0
4	35.67192181151835	37.0	35.0	37.0	33.0	37.0
5	33.608718782709495	37.0	35.0	37.0	32.0	37.0
6	34.58913850226209	37.0	35.0	37.0	30.0	37.0
7	35.699361020521735	37.0	35.0	37.0	32.0	37.0
8	35.72402776215892	37.0	35.0	37.0	35.0	37.0
9	37.60319835756899	39.0	37.0	39.0	35.0	39.0
10	36.908756556315836	39.0	37.0	39.0	32.0	39.0
11	37.03702390117733	39.0	37.0	39.0	34.0	39.0
12	37.09569698070663	39.0	37.0	39.0	34.0	39.0
13	37.14318936110703	39.0	37.0	39.0	33.0	39.0
14	38.23045648394045	40.0	38.0	41.0	33.0	41.0
15	38.33549161916131	40.0	38.0	41.0	34.0	41.0
16	38.299335645885414	40.0	37.0	41.0	34.0	41.0
17	38.294285228213134	40.0	37.0	41.0	34.0	41.0
18	38.25829217163635	40.0	37.0	41.0	34.0	41.0
19	38.161979567650796	40.0	37.0	41.0	34.0	41.0
20	38.25095371147309	40.0	37.0	41.0	34.0	41.0
21	38.157987387652355	40.0	37.0	41.0	34.0	41.0
22	38.218885073081836	40.0	37.0	41.0	34.0	41.0
23	38.231488770281686	40.0	37.0	41.0	34.0	41.0
24	38.186124399154565	40.0	37.0	41.0	34.0	41.0
25	37.996404298694074	40.0	37.0	41.0	34.0	41.0
26	38.074969074661986	40.0	37.0	41.0	34.0	41.0
27	38.076888031533755	40.0	37.0	41.0	34.0	41.0
28	37.98569072355199	40.0	37.0	41.0	34.0	41.0
29	37.99088819877567	40.0	37.0	41.0	34.0	41.0
30	37.84748690178574	40.0	37.0	41.0	33.0	41.0
31	37.82296577595926	40.0	37.0	41.0	33.0	41.0
32	37.76989241730896	40.0	37.0	41.0	33.0	41.0
33	37.701209332099204	40.0	37.0	41.0	33.0	41.0
34	37.652726475837866	40.0	37.0	41.0	33.0	41.0
35	37.59985784436697	40.0	37.0	41.0	33.0	41.0
36	37.51039495044737	40.0	36.0	41.0	33.0	41.0
37	37.486219842388905	40.0	36.0	41.0	33.0	41.0
38	37.367721732510965	40.0	36.0	41.0	33.0	41.0
39	37.31604253711762	40.0	36.0	41.0	32.0	41.0
40	37.294518790206546	40.0	36.0	41.0	32.0	41.0
41	37.223350144029894	40.0	36.0	41.0	32.0	41.0
42	37.23071743900716	40.0	36.0	41.0	32.0	41.0
43	37.152938411720775	39.0	36.0	41.0	32.0	41.0
44	37.01365328442949	39.0	35.0	41.0	32.0	41.0
45	36.94587417063866	39.0	35.0	41.0	31.0	41.0
46	36.87054610254236	39.0	35.0	41.0	31.0	41.0
47	36.79709373909684	39.0	35.0	41.0	31.0	41.0
48	36.714391167319775	39.0	35.0	41.0	31.0	41.0
49	36.614394915845594	39.0	35.0	41.0	31.0	41.0
50	36.50412337840215	39.0	35.0	41.0	31.0	41.0
51	35.330387857083124	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	13.0
11	11.0
12	15.0
13	9.0
14	17.0
15	10.0
16	42.0
17	58.0
18	120.0
19	221.0
20	428.0
21	732.0
22	1114.0
23	1595.0
24	2412.0
25	3303.0
26	4558.0
27	5463.0
28	6327.0
29	7715.0
30	9663.0
31	12477.0
32	16589.0
33	23694.0
34	38485.0
35	50847.0
36	54523.0
37	84407.0
38	148218.0
39	220416.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75077493562628	20.929173046369264	23.226442677831507	12.09360934017295
2	31.82714682398941	25.219937543792874	24.89078814197109	18.062127490246624
3	31.850935545540267	24.858493150289934	26.32993370876261	16.960637595407192
4	28.170748234588512	26.32878031620257	26.447579749886824	19.052891699322096
5	23.92597526549655	34.47692205661427	23.31539807902469	18.281704598864483
6	24.378105149032734	36.398185713503054	24.719941869014974	14.503767268449236
7	80.23993448730259	6.032387263085959	9.164280585808081	4.56339766380337
8	81.68527953910434	5.197619397756075	8.076919749829154	5.0401813133104385
9	75.92912979414827	6.921364578737784	10.352419096720617	6.797086530393336
10	36.93696421311235	24.87737995346061	21.580551494652582	16.605104338774463
11	29.443805272734085	26.33209631981269	23.882290522284986	20.341807885168237
12	24.302125414140015	23.55285277232319	30.22090351006191	21.92411830347488
13	24.21360253515685	25.006559920185232	32.374143245588996	18.405694299068923
14	22.16258221526342	28.203187400339676	29.451590672514367	20.182639711882537
15	20.750685547702872	26.59074460140195	33.419981949406434	19.238587901488742
16	22.867737591658667	26.072727167873406	30.425630689469234	20.633904550998693
17	23.29665544992402	26.02341963593164	28.25177406193141	22.428150852212926
18	23.28295891327353	25.349117510517498	30.55639657096392	20.811527005245054
19	22.761769650204872	28.13268628010715	28.211982018609987	20.89356205107799
20	23.378690495756956	27.515477086415054	28.635277088145145	20.470555329682846
21	22.79997577875624	28.651280409915714	29.39247930381225	19.156264507515793
22	21.39975721086611	26.556286998670714	28.27239095394215	23.771564836521023
23	20.43465598625156	27.986349599051913	28.76186192160968	22.817132493086852
24	21.338050708903904	26.059751501572936	29.776559026306	22.825638763217157
25	20.443450604521875	28.30281168271324	28.045749315894035	23.207988396870846
26	19.8911197423321	27.78652433802476	29.150699388413592	23.171656531229544
27	22.159266211653303	27.32213965853813	28.11682713240658	22.401766997401985
28	20.225315236604064	26.778603414618672	29.88829393056	23.10778741821726
29	21.23684051176028	26.208394967748262	28.02657416458335	24.52819035590811
30	23.12523248068788	27.224245465004625	27.18776942529332	22.462752629014165
31	23.379123017966972	27.314065910617845	25.543031634674442	23.76377943674074
32	22.633166379760265	29.04646153579986	25.680717871529367	22.6396542129105
33	22.512492683165945	28.45001340818851	25.942970504868757	23.094523403776783
34	21.202527083099053	27.410085841241283	26.415717280415684	24.971669795243987
35	20.63736472867882	28.76344783637973	25.990259599830452	24.608927835111
36	22.445740088753556	29.889014800910026	25.30615363765596	22.359091472680458
37	22.13576583824246	29.493977849095888	26.582526679411657	21.78772963325
38	20.67095728699002	31.744967604086472	25.290438664025398	22.293636444898112
39	20.811527005245054	29.88382453438984	26.270533991920487	23.03411446844462
40	20.62424488830835	28.211837844539982	27.250485145745568	23.9134321214061
41	20.075806726008715	28.46789099286915	25.258576194554255	26.19772608656788
42	21.460166146198272	27.935167804200077	26.704497942636017	23.900168106965623
43	22.525324175396406	27.03696334806792	27.10501350911036	23.332698967425312
44	21.445892913267762	27.300225199897348	26.638033696363646	24.615848190471247
45	21.387502414915673	26.462141330957344	26.27745434728073	25.872901906846252
46	22.898446668569765	27.602125702488156	25.496751758202784	24.002675870739296
47	20.081285340668913	27.35227203916921	28.42233198674752	24.14411063341436
48	21.20339212751908	26.640340481483726	27.65446088990003	24.501806501097164
49	21.66229819234551	25.651306361248317	29.212405890375802	23.473989556030368
50	20.431339982641443	25.444416570790914	28.561027442092485	25.563216004475166
51	20.080852818458894	25.237959302543516	26.48650674878822	28.19468113020937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	364.0
2	535.0
3	1616.0
4	2697.0
5	1879.0
6	1061.0
7	1075.0
8	1089.0
9	1122.5
10	1156.0
11	1147.0
12	1138.0
13	1137.5
14	1137.0
15	1245.0
16	1353.0
17	1257.0
18	1161.0
19	1357.0
20	1553.0
21	1606.0
22	1659.0
23	1904.5
24	2150.0
25	2686.5
26	4043.5
27	4864.0
28	5888.0
29	6912.0
30	8320.5
31	9729.0
32	11879.0
33	14029.0
34	15547.0
35	17065.0
36	19151.5
37	21238.0
38	23961.5
39	26685.0
40	30346.5
41	34008.0
42	37916.0
43	41824.0
44	46493.0
45	51162.0
46	62283.5
47	73405.0
48	76554.0
49	79703.0
50	79394.0
51	79085.0
52	69564.0
53	60043.0
54	51549.0
55	43055.0
56	37698.0
57	32341.0
58	27983.0
59	23625.0
60	21045.5
61	18466.0
62	16074.5
63	13683.0
64	11264.5
65	8846.0
66	7142.0
67	5438.0
68	4433.0
69	3428.0
70	2531.0
71	1634.0
72	1424.5
73	1215.0
74	1114.0
75	825.0
76	637.0
77	410.0
78	183.0
79	127.5
80	72.0
81	62.5
82	53.0
83	47.5
84	42.0
85	24.5
86	7.0
87	4.0
88	1.0
89	2.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	693606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.38304594516889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28444033962383	27.022125543369775
2	11.278041918503723	8.432151184219308
3	4.107236008228664	4.606229772097927
4	2.132799834457516	3.189222168134957
5	1.3309781255927056	2.4878008210523443
6	0.9906132361245235	2.22192840719613
7	0.8213286720226256	2.149263723761445
8	0.671823892955578	2.009185876593648
9	0.5766261168208078	1.9400436556466925
>10	5.70247748488202	40.055206234123105
>50	0.06740130159948651	1.7053623456426061
>100	0.03233704024352545	2.1950222612842496
>500	0.0011688086835009194	0.2631812903019467
>1k	0.0027272202615021453	1.7232767165758902
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	2263	0.32626592042168034	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	1976	0.284887962330199	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	1917	0.2763816921998945	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	1598	0.23039016386824798	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1567	0.22592076769808797	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.19045394647681824	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	1190	0.17156714330614212	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4417407000516142E-4	0.0	0.0	0.17920836901641565	0.0
2	1.4417407000516142E-4	0.0	0.0	0.9302110996733015	0.0
3	1.4417407000516142E-4	0.0	0.0	1.3116956889069586	0.0
4	1.4417407000516142E-4	0.0	0.0	1.8651799436567735	0.0
5	1.4417407000516142E-4	0.0	0.0	3.450806365573539	0.0
6	1.4417407000516142E-4	0.0	0.0	4.521875531641883	0.0
7	1.4417407000516142E-4	0.0	0.0	5.516099918397477	0.0
8	1.4417407000516142E-4	0.0	0.0	7.124361669305052	0.0
9	1.4417407000516142E-4	0.0	0.0	7.683324538715063	0.0
10	1.4417407000516142E-4	0.0	0.0	9.196287229349227	0.0
11	1.4417407000516142E-4	0.0	0.0	10.86063269348881	0.0
12	1.4417407000516142E-4	0.0	0.0	12.461397392756117	0.0
13	1.4417407000516142E-4	0.0	0.0	13.064477527587709	0.0
14	1.4417407000516142E-4	0.0	0.0	13.308852576246457	0.0
15	1.4417407000516142E-4	0.0	0.0	13.750025230462251	0.0
16	1.4417407000516142E-4	0.0	0.0	14.668846578605145	0.0
17	1.4417407000516142E-4	0.0	0.0	15.791241713595326	0.0
18	1.4417407000516142E-4	0.0	0.0	16.972459869147613	0.0
19	2.8834814001032285E-4	0.0	0.0	17.72951791074472	0.0
20	2.8834814001032285E-4	0.0	0.0	18.416939876529327	0.0
21	2.8834814001032285E-4	0.0	0.0	19.288760477850538	0.0
22	2.8834814001032285E-4	0.0	0.0	20.183072234092553	0.0
23	2.8834814001032285E-4	0.0	0.0	21.117752729936015	0.0
24	2.8834814001032285E-4	0.0	0.0	21.859672494182576	0.0
25	2.8834814001032285E-4	0.0	0.0	22.535416360296768	0.0
26	2.8834814001032285E-4	0.0	0.0	23.186218112300068	0.0
27	2.8834814001032285E-4	0.0	0.0	23.825918460912966	0.0
28	2.8834814001032285E-4	0.0	0.0	24.486668223746623	0.0
29	2.8834814001032285E-4	0.0	0.0	25.142948590410118	0.0
30	2.8834814001032285E-4	0.0	0.0	25.8743436475463	0.0
31	2.8834814001032285E-4	0.0	0.0	26.597664956762195	0.0
32	2.8834814001032285E-4	0.0	0.0	27.26374916018604	0.0
33	2.8834814001032285E-4	0.0	0.0	27.924354748949693	0.0
34	2.8834814001032285E-4	0.0	0.0	28.52008200621102	0.0
35	2.8834814001032285E-4	0.0	0.0	29.18602203556486	0.0
36	2.8834814001032285E-4	0.0	0.0	29.805249666237028	0.0
37	2.8834814001032285E-4	0.0	0.0	30.440913140889784	0.0
38	2.8834814001032285E-4	0.0	0.0	31.069800434252297	0.0
39	2.8834814001032285E-4	0.0	0.0	31.711519219845272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.031349E-4	45.000004	1
AATCCGC	20	7.031349E-4	45.000004	37
TTTCGCG	20	7.031349E-4	45.000004	1
CACGCAG	20	7.031349E-4	45.000004	19
AACGGTT	20	7.031349E-4	45.000004	26
CCCGTCG	20	7.031349E-4	45.000004	18
TCGACAA	20	7.031349E-4	45.000004	19
GTACGCG	20	7.031349E-4	45.000004	1
CCGCTAT	25	3.8892E-5	45.0	42
TCATGCG	25	3.8892E-5	45.0	1
GCGATTT	45	3.8380676E-10	45.0	9
TACACGA	25	3.8892E-5	45.0	37
CCCGCAT	25	3.8892E-5	45.0	23
CGTAAGG	165	0.0	42.272728	2
ATGCGAG	65	0.0	41.53846	1
CGATGCG	60	3.6379788E-12	41.249996	10
CTCGCCT	50	1.0804797E-9	40.5	27
TATCGGG	50	1.0804797E-9	40.5	3
CACTCGC	40	3.4555705E-7	39.375004	25
GCGTAAG	80	0.0	39.375004	1
>>END_MODULE