##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935880.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678011
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89377753458277	33.0	31.0	34.0	30.0	34.0
2	31.93625914623804	33.0	31.0	34.0	30.0	34.0
3	31.980869041947695	33.0	31.0	34.0	30.0	34.0
4	35.678878366280195	37.0	35.0	37.0	33.0	37.0
5	33.60873201172252	37.0	35.0	37.0	32.0	37.0
6	34.585869550789	37.0	35.0	37.0	30.0	37.0
7	35.68192403958048	37.0	35.0	37.0	32.0	37.0
8	35.690182017695875	37.0	35.0	37.0	35.0	37.0
9	37.5536650585315	39.0	37.0	39.0	35.0	39.0
10	37.121748762188226	39.0	37.0	39.0	34.0	39.0
11	37.096640025014345	39.0	37.0	39.0	34.0	39.0
12	37.03626932306408	39.0	37.0	39.0	33.0	39.0
13	36.971997504465264	39.0	37.0	39.0	33.0	39.0
14	38.006044149726186	40.0	37.0	41.0	33.0	41.0
15	38.093678421146556	40.0	37.0	41.0	33.0	41.0
16	38.108838942141055	40.0	37.0	41.0	33.0	41.0
17	38.09925945154282	40.0	37.0	41.0	33.0	41.0
18	38.08500009586865	40.0	37.0	41.0	34.0	41.0
19	38.01621212635193	40.0	37.0	41.0	34.0	41.0
20	38.05063192190097	40.0	37.0	41.0	34.0	41.0
21	37.95777649625153	40.0	36.0	41.0	33.0	41.0
22	38.02043772151189	40.0	36.0	41.0	34.0	41.0
23	38.01209567396399	40.0	36.0	41.0	34.0	41.0
24	37.96530439771626	40.0	36.0	41.0	34.0	41.0
25	37.756853502376806	40.0	36.0	41.0	33.0	41.0
26	37.8354156495986	40.0	36.0	41.0	33.0	41.0
27	37.8379038098202	40.0	36.0	41.0	33.0	41.0
28	37.747700258550374	40.0	36.0	41.0	33.0	41.0
29	37.747684034624804	40.0	36.0	41.0	33.0	41.0
30	37.61190010191575	40.0	36.0	41.0	33.0	41.0
31	37.60619812952887	40.0	36.0	41.0	33.0	41.0
32	37.55990684516918	40.0	36.0	41.0	33.0	41.0
33	37.49766449216901	40.0	36.0	41.0	33.0	41.0
34	37.41203018830078	40.0	35.0	41.0	33.0	41.0
35	37.33754909581113	40.0	35.0	41.0	33.0	41.0
36	37.24772164463408	40.0	35.0	41.0	32.0	41.0
37	37.21545963118592	40.0	35.0	41.0	32.0	41.0
38	37.13072796754035	39.0	35.0	41.0	32.0	41.0
39	37.0792936987748	39.0	35.0	41.0	32.0	41.0
40	36.98314039152757	39.0	35.0	41.0	32.0	41.0
41	36.89347517960623	39.0	35.0	41.0	31.0	41.0
42	36.88711245097793	39.0	35.0	41.0	32.0	41.0
43	36.80520080057698	39.0	35.0	41.0	31.0	41.0
44	36.66807028204557	39.0	35.0	41.0	31.0	41.0
45	36.634740439314406	39.0	35.0	41.0	31.0	41.0
46	36.547645982144836	39.0	35.0	41.0	31.0	41.0
47	36.45499409301619	39.0	35.0	40.0	31.0	41.0
48	36.37151462144419	38.0	35.0	40.0	31.0	41.0
49	36.27612678850343	38.0	35.0	40.0	31.0	41.0
50	36.160631612171485	38.0	35.0	40.0	30.0	41.0
51	34.921520447308374	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	20.0
10	12.0
11	15.0
12	10.0
13	13.0
14	19.0
15	19.0
16	45.0
17	70.0
18	154.0
19	258.0
20	523.0
21	853.0
22	1220.0
23	1855.0
24	2580.0
25	3442.0
26	4654.0
27	5645.0
28	6573.0
29	8097.0
30	10354.0
31	13280.0
32	17681.0
33	25612.0
34	44677.0
35	56164.0
36	53415.0
37	81761.0
38	136732.0
39	202143.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5211065897161	19.541275879004914	23.464663552656226	12.472953978622765
2	30.706286476178114	25.96831024865378	25.2457555998354	18.0796476753327
3	32.16201507055195	25.0675873990245	25.230711596124543	17.539685934299
4	29.20822818508844	24.981453103268237	26.464762371111973	19.34555634053135
5	24.295476032099774	33.5146479924367	23.130450685903327	19.0594252895602
6	25.57023411124598	35.21344048990356	24.19827996890906	15.0180454299414
7	79.46080520817509	5.72453839244496	9.985973678893115	4.828682720486835
8	79.79398564330077	6.067010712215583	8.43806368923218	5.700939955251464
9	73.72579500922552	7.204602875174591	10.653956941701535	8.415645173898358
10	40.9732290479063	25.299589534683065	17.37729918836125	16.34988222904938
11	30.656139797141936	24.17025682474178	24.684555265327553	20.48904811278873
12	28.15868769090767	21.04538126962542	29.88609329347164	20.909837745995272
13	25.45991141736639	22.655532137384203	31.824557418684947	20.059999026564466
14	21.558647278583976	26.859446233173205	29.481675076068086	22.10023141217473
15	19.17092790529947	24.666266476502592	35.54868578828367	20.614119829914266
16	22.3946219161636	23.244460635594407	31.698158289467283	22.66275915877471
17	22.377955519895696	23.916573624911692	28.611040233860514	25.094430621332105
18	23.549765416785274	24.04002294948017	29.89346780509461	22.51674382863995
19	23.93677978675862	26.32818641585461	26.859741213638127	22.875292583748642
20	25.637784637712368	24.770984541548735	28.174469145780822	21.416761674958078
21	24.732194610411927	26.42405506695319	28.012819851005368	20.830930471629514
22	22.853464029344657	23.347408817851036	28.145856040683704	25.653271112120603
23	22.106426001938022	26.052527171388075	28.269600345717105	23.571446480956798
24	23.491506774963828	23.205818194690057	28.85144931276926	24.451225717576854
25	22.57308509743942	25.92966780774943	26.80133508158422	24.695912013226923
26	21.807463300742906	25.85312037710303	27.354570943539265	24.9848453786148
27	22.240937093940953	25.83365166641839	27.37374467375898	24.551666565881675
28	20.04451255215623	25.80548103201866	28.702336687752855	25.44766972807226
29	22.75435059313197	23.751827035254593	28.928881684810428	24.564940686803016
30	23.144462257986966	25.254457523550506	27.155311639486673	24.44576857897586
31	23.24239577233998	25.301801888169955	26.06270399742777	25.3930983420623
32	24.826145888488536	26.394409530228863	25.46330369271295	23.316140888569656
33	23.624985435339543	25.50755076245076	25.445457374585366	25.422006427624332
34	22.98591025809316	24.587801672834217	28.173289223921145	24.25299884515148
35	22.25509615625705	25.805923502716034	27.196756394807753	24.74222394621916
36	24.799450156413393	25.6619730358357	26.240282237308833	23.29829457044207
37	22.07265073870483	26.438214129269287	28.347622678688104	23.14151245333778
38	21.53283648790359	27.30294936217849	25.71949422649485	25.44471992342307
39	23.248885342568187	24.762725088531013	28.37549833262292	23.61289123627788
40	22.64270048716024	25.309176399792925	29.142005070714195	22.906118042332647
41	21.149509373741722	25.633654911203507	27.397343110952477	25.819492604102294
42	23.346228895991363	25.026732604633256	27.23421891385243	24.392819585522947
43	23.737667972938493	23.93265006024976	27.089236015344888	25.240445951466867
44	21.752744424500488	24.303440504652578	27.99217121846106	25.951643852385875
45	21.330627379201815	23.739880326425382	27.578461116412566	27.351031177960238
46	23.836191448221342	25.289855179340748	27.228171814321595	23.64578155811631
47	20.949365128294378	24.975111023272483	29.952611388310807	24.12291246012233
48	22.00318283921647	23.943416847219293	28.627559139895958	25.425841173668275
49	22.12515726146036	22.62588660065987	30.625314338557928	24.62364179932184
50	21.37502193917208	22.32869378225427	30.178271443973625	26.11801283460003
51	19.867966743902386	23.02632258178702	27.965917957083292	29.1397927172273
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	626.0
2	949.0
3	1753.0
4	2557.0
5	1777.5
6	998.0
7	999.5
8	1001.0
9	1081.0
10	1161.0
11	1105.5
12	1050.0
13	1092.5
14	1135.0
15	1114.5
16	1094.0
17	1178.0
18	1262.0
19	1223.0
20	1184.0
21	1164.0
22	1144.0
23	1606.5
24	2069.0
25	2647.5
26	3809.0
27	4392.0
28	4876.0
29	5360.0
30	6534.5
31	7709.0
32	8796.5
33	9884.0
34	11625.5
35	13367.0
36	15664.5
37	17962.0
38	19699.5
39	21437.0
40	23705.5
41	25974.0
42	29533.5
43	33093.0
44	38377.0
45	43661.0
46	55696.0
47	67731.0
48	69186.5
49	70642.0
50	70035.5
51	69429.0
52	62098.5
53	54768.0
54	48801.0
55	42834.0
56	40808.5
57	38783.0
58	35543.0
59	32303.0
60	30723.5
61	29144.0
62	26051.5
63	22959.0
64	20662.0
65	18365.0
66	14573.0
67	10781.0
68	8800.5
69	6820.0
70	5703.5
71	4587.0
72	3722.5
73	2858.0
74	2432.5
75	1516.0
76	1025.0
77	795.5
78	566.0
79	410.5
80	255.0
81	168.5
82	82.0
83	54.5
84	27.0
85	30.0
86	33.0
87	28.0
88	23.0
89	15.0
90	7.0
91	6.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	678011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.65114598192466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.19232897051039	21.821264680683182
2	11.741088066248846	7.197556086104538
3	4.5043303304300855	4.1418865952647055
4	2.2394742002520807	2.7456980253872203
5	1.2639700592670455	1.9371065401688088
6	0.8460195711281904	1.5558881626929275
7	0.5976181425653603	1.2822376650452265
8	0.47369892035125205	1.1615531807333066
9	0.39782764260564163	1.0974485834235426
>10	6.431700645835341	47.190816180498686
>50	0.2622063371333665	4.8122381826623215
>100	0.043459613888547154	2.2604035777749583
>500	0.00289730759256981	0.5110773673426487
>1k	0.0033801921913314452	2.284825172217917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	3303	0.4871602378132508	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	3032	0.44719038481676554	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	2772	0.40884292437733305	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2420	0.35692636255164	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	1533	0.2261025263601918	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1214	0.17905314220565743	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	1163	0.17153114035023032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9498046491871076E-4	0.0	0.0	0.18392031987681615	0.0
2	2.9498046491871076E-4	0.0	0.0	0.9629637277271312	0.0
3	2.9498046491871076E-4	0.0	0.0	1.2782978447252331	0.0
4	2.9498046491871076E-4	0.0	0.0	1.7959885606575705	0.0
5	2.9498046491871076E-4	0.0	0.0	3.367496987512002	0.0
6	2.9498046491871076E-4	0.0	0.0	4.255093206452402	0.0
7	2.9498046491871076E-4	0.0	0.0	5.125285577962599	0.0
8	2.9498046491871076E-4	0.0	0.0	6.518330823541211	0.0
9	2.9498046491871076E-4	0.0	0.0	6.992954391595417	0.0
10	2.9498046491871076E-4	0.0	0.0	8.453697653872872	0.0
11	2.9498046491871076E-4	0.0	0.0	9.89098996918929	0.0
12	2.9498046491871076E-4	0.0	0.0	11.61175851129259	0.0
13	2.9498046491871076E-4	0.0	0.0	12.146115623492834	0.0
14	2.9498046491871076E-4	0.0	0.0	12.364696147997599	0.0
15	2.9498046491871076E-4	0.0	0.0	12.787403154226112	0.0
16	2.9498046491871076E-4	0.0	0.0	13.555089814177055	0.0
17	2.9498046491871076E-4	0.0	0.0	14.486195651692967	0.0
18	2.9498046491871076E-4	0.0	0.0	15.466268246385383	0.0
19	2.9498046491871076E-4	0.0	0.0	16.13911868686496	0.0
20	2.9498046491871076E-4	0.0	0.0	16.75754523156704	0.0
21	2.9498046491871076E-4	0.0	0.0	17.479657409688045	0.0
22	2.9498046491871076E-4	0.0	0.0	18.270795016600026	0.0
23	4.4247069737806614E-4	0.0	0.0	19.00662378633975	0.0
24	4.4247069737806614E-4	0.0	0.0	19.58758781199715	0.0
25	4.4247069737806614E-4	0.0	0.0	20.09775652607406	0.0
26	4.4247069737806614E-4	0.0	0.0	20.61249743735721	0.0
27	4.4247069737806614E-4	0.0	0.0	21.100542616565217	0.0
28	4.4247069737806614E-4	0.0	0.0	21.59935458274276	0.0
29	4.4247069737806614E-4	0.0	0.0	22.128697027039383	0.0
30	4.4247069737806614E-4	0.0	0.0	22.712463367113514	0.0
31	4.4247069737806614E-4	0.0	0.0	23.245198086756705	0.0
32	5.899609298374215E-4	0.0	0.0	23.74518997479392	0.0
33	7.374511622967769E-4	0.0	0.0	24.276892262809895	0.0
34	7.374511622967769E-4	0.0	0.0	24.82688333965083	0.0
35	7.374511622967769E-4	0.0	0.0	25.41507438669874	0.0
36	8.849413947561323E-4	0.0	0.0	25.9457442430875	0.0
37	8.849413947561323E-4	0.0	0.0	26.466089783204108	0.0
38	0.0010324316272154876	0.0	0.0	26.977438419140693	0.0
39	0.0010324316272154876	0.0	0.0	27.52521714249474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.0312363E-4	45.000004	1
ATTACGG	25	3.8891063E-5	45.000004	2
TAGCACG	20	7.0312363E-4	45.000004	1
CCGAATC	20	7.0312363E-4	45.000004	13
GCGTAAG	25	3.8891063E-5	45.000004	1
CTAATCG	20	7.0312363E-4	45.000004	44
CGTATGG	55	1.8189894E-12	45.0	2
GTTAACG	30	2.164179E-6	44.999996	1
CCCCGAT	60	3.6379788E-12	41.249996	14
TGTTGCG	50	1.0804797E-9	40.500004	1
TGTACCG	50	1.0804797E-9	40.500004	1
CTCACGA	45	1.9263098E-8	40.0	24
AACCCGC	40	3.455425E-7	39.375004	21
CCCATTA	110	0.0	38.863636	33
CATACGA	105	0.0	38.57143	18
GTTAGCG	35	6.244498E-6	38.571426	1
ACGATAT	35	6.244498E-6	38.571426	23
TACGATA	35	6.244498E-6	38.571426	22
TAGGCCG	35	6.244498E-6	38.571426	1
CGATATA	35	6.244498E-6	38.571426	24
>>END_MODULE