##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935879.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739274
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775199722971454	33.0	31.0	34.0	30.0	34.0
2	31.816804053706743	33.0	31.0	34.0	30.0	34.0
3	31.84509261789269	33.0	31.0	34.0	30.0	34.0
4	35.56786522994181	37.0	35.0	37.0	33.0	37.0
5	33.479861323406475	37.0	35.0	37.0	31.0	37.0
6	34.4739501186299	37.0	35.0	37.0	30.0	37.0
7	35.61673479657069	37.0	35.0	37.0	32.0	37.0
8	35.66990452795581	37.0	35.0	37.0	33.0	37.0
9	37.53644386249212	39.0	37.0	39.0	35.0	39.0
10	36.83732012758463	39.0	37.0	39.0	32.0	39.0
11	36.95009428168717	39.0	37.0	39.0	33.0	39.0
12	37.047600754253494	39.0	37.0	39.0	33.0	39.0
13	37.09329558458704	39.0	37.0	39.0	33.0	39.0
14	38.152614592153924	40.0	37.0	41.0	33.0	41.0
15	38.25193365382795	40.0	37.0	41.0	34.0	41.0
16	38.222498018326085	40.0	37.0	41.0	34.0	41.0
17	38.2232839244989	40.0	37.0	41.0	34.0	41.0
18	38.18788432976136	40.0	37.0	41.0	34.0	41.0
19	38.091619616001644	40.0	37.0	41.0	34.0	41.0
20	38.148333364895834	40.0	37.0	41.0	34.0	41.0
21	38.05141801280716	40.0	37.0	41.0	34.0	41.0
22	38.14192978516761	40.0	37.0	41.0	34.0	41.0
23	38.14618666421381	40.0	37.0	41.0	34.0	41.0
24	38.10352589161799	40.0	37.0	41.0	34.0	41.0
25	37.899241688467335	40.0	37.0	41.0	33.0	41.0
26	37.99492745585534	40.0	37.0	41.0	34.0	41.0
27	37.97868990387867	40.0	37.0	41.0	34.0	41.0
28	37.835310047424905	40.0	37.0	41.0	33.0	41.0
29	37.83195946293255	40.0	37.0	41.0	33.0	41.0
30	37.70544209589408	40.0	36.0	41.0	33.0	41.0
31	37.66473188560669	40.0	36.0	41.0	33.0	41.0
32	37.655804207912084	40.0	37.0	41.0	33.0	41.0
33	37.55690582923246	40.0	37.0	41.0	33.0	41.0
34	37.493105397998576	40.0	37.0	41.0	33.0	41.0
35	37.45342187064607	40.0	37.0	41.0	33.0	41.0
36	37.35031801470091	40.0	36.0	41.0	32.0	41.0
37	37.31328303173113	40.0	36.0	41.0	32.0	41.0
38	37.21009530972278	40.0	36.0	41.0	32.0	41.0
39	37.17741459864678	40.0	36.0	41.0	32.0	41.0
40	37.15084934679158	40.0	36.0	41.0	32.0	41.0
41	37.028546925767714	39.0	36.0	41.0	31.0	41.0
42	37.04397016532436	39.0	36.0	41.0	31.0	41.0
43	36.98154811341938	39.0	36.0	41.0	31.0	41.0
44	36.86762418264405	39.0	35.0	41.0	31.0	41.0
45	36.8477966220914	39.0	35.0	41.0	31.0	41.0
46	36.736653798185785	39.0	35.0	41.0	31.0	41.0
47	36.69628987357867	39.0	35.0	41.0	31.0	41.0
48	36.605878199422676	39.0	35.0	41.0	31.0	41.0
49	36.4953224379594	39.0	35.0	41.0	30.0	41.0
50	36.39235790789342	39.0	35.0	41.0	30.0	41.0
51	35.142622356528165	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	10.0
11	6.0
12	8.0
13	8.0
14	13.0
15	20.0
16	33.0
17	75.0
18	123.0
19	264.0
20	498.0
21	796.0
22	1264.0
23	1948.0
24	2867.0
25	4361.0
26	5710.0
27	6748.0
28	7595.0
29	8995.0
30	11071.0
31	14242.0
32	18928.0
33	25678.0
34	40786.0
35	53682.0
36	58410.0
37	89254.0
38	157460.0
39	228324.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18097755365399	20.923230087897046	24.681647129481085	12.21414522896788
2	31.21521925564811	25.385039917540723	26.049340298725504	17.350400528085665
3	30.626263063492022	24.723174357545375	27.969061538752882	16.68150104020972
4	27.227387950881543	25.67153721083117	28.428972207868803	18.67210263041849
5	23.39322091673723	33.43307082353768	24.63687347316421	18.536834786560867
6	23.09630799946975	35.98814512616432	26.047581816755354	14.867965057610574
7	79.71509886726707	5.382713310626372	10.368686035218335	4.533501786888217
8	80.7642362642268	4.858956219209657	8.66214691711057	5.714660599452976
9	74.82083774081057	6.746618980242778	11.566888596109155	6.865654682837487
10	35.49685231727343	26.91207860684942	21.281013534900456	16.310055540976688
11	26.022016194266268	26.493018826578506	27.115791979699	20.36917299945622
12	22.400896014197713	21.97331435976377	33.090166839358616	22.535622786679905
13	22.680765183139133	25.16090110026864	34.61896401063746	17.539369705954762
14	20.19711230206933	28.85073734501687	31.15840676122791	19.793743591685896
15	18.427944172255483	27.51145042298255	35.811891125617834	18.24871427914413
16	20.569504676209363	26.63004515240628	31.196687561039617	21.60376261034474
17	20.79837786801646	26.807110760015906	29.073252948162658	23.321258423804977
18	22.019034890987648	25.36474974096208	32.166016930123334	20.45019843792694
19	21.547761722987687	28.58344808555421	28.673942273094955	21.194847918363152
20	21.219737201632956	27.507121851979104	30.22789926333132	21.04524168305662
21	20.71356492991773	29.26262792956333	30.735559481328977	19.28824765918996
22	19.246585163281814	25.970343877912654	29.718615831207373	25.064455127598155
23	17.8843027077917	28.039130281871135	29.729707794403698	24.34685921593347
24	19.714882438716906	26.20219296228462	30.613547886169407	23.469376712829074
25	18.733243695842138	28.91187841044051	28.748069051528937	23.606808842188418
26	18.287941953862845	28.068212868300524	30.405370674472522	23.238474503364113
27	21.333632726161074	26.71215273362786	28.665420398931925	23.288794141279144
28	18.65817004250116	26.495453647767945	31.816349553751383	23.030026755979517
29	19.26389944729559	24.4725230428772	29.655716283813575	26.60786122601363
30	21.883631779286166	25.582936773104425	28.392044086495673	24.141387361113743
31	19.949572147809878	26.373712588296083	29.033484202068514	24.64323106182552
32	19.604098074597513	27.43407721629599	26.985393778220253	25.976430930886245
33	19.916972597440193	26.19583537362331	27.512262030045694	26.374929998890806
34	18.21638526446216	25.256535465875977	30.455284508855986	26.071794760805872
35	18.80128342130252	26.371548302794363	27.49941158487922	27.327756691023897
36	20.326293092953357	27.119308943639297	28.43654720712483	24.117850756282515
37	18.567134783584976	26.8096808490492	31.570973684993657	23.052210682372166
38	17.732802722671163	28.394208371997394	28.201857498031853	25.671131407299598
39	19.19897088224393	26.066789850583138	29.158742225480673	25.575497041692252
40	19.919272150785773	25.407088576089514	29.657339497939873	25.01629977518484
41	18.063262065215334	27.218595541030794	28.501069968644916	26.217072425108956
42	20.056163208769682	27.09590760665193	29.695079226376148	23.15284995820224
43	20.69598011021624	25.93706798832368	29.571850220621855	23.795101680838226
44	19.401466844498792	25.23556895007805	28.893617251519732	26.469346953903422
45	19.43798916234035	23.72787356244099	28.17696821476205	28.65716906045661
46	21.715223313683424	25.50826892329502	28.385957033522075	24.390550729499484
47	18.613125850496566	24.877785503074637	31.49671163871582	25.01237700771297
48	18.87243430717163	25.09610780306084	29.637049321361225	26.394408568406302
49	19.95349491528175	23.023263363786633	32.004912928088906	25.01832879284271
50	19.31787131699478	22.53656966158691	31.104705427216434	27.04085359420188
51	18.994986973706638	22.78559776212879	28.196717319965263	30.02269794419931
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	319.5
2	496.0
3	2140.0
4	3784.0
5	2643.0
6	1502.0
7	1496.0
8	1490.0
9	1494.0
10	1498.0
11	1514.0
12	1530.0
13	1539.0
14	1548.0
15	1476.5
16	1405.0
17	1472.0
18	1539.0
19	1593.0
20	1647.0
21	1832.5
22	2018.0
23	2427.5
24	2837.0
25	3259.5
26	4778.5
27	5875.0
28	6814.5
29	7754.0
30	9167.5
31	10581.0
32	12891.0
33	15201.0
34	17540.0
35	19879.0
36	21975.0
37	24071.0
38	25976.5
39	27882.0
40	32323.5
41	36765.0
42	40595.5
43	44426.0
44	51051.0
45	57676.0
46	72017.0
47	86358.0
48	90842.5
49	95327.0
50	92962.5
51	90598.0
52	77035.0
53	63472.0
54	53608.5
55	43745.0
56	36686.0
57	29627.0
58	25045.5
59	20464.0
60	17243.0
61	14022.0
62	11335.5
63	8649.0
64	7002.0
65	5355.0
66	4031.5
67	2708.0
68	2147.5
69	1587.0
70	1247.0
71	907.0
72	703.0
73	499.0
74	374.0
75	250.5
76	252.0
77	190.0
78	128.0
79	83.5
80	39.0
81	38.5
82	38.0
83	20.0
84	2.0
85	6.5
86	11.0
87	7.0
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	739274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.40060969006363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45390869760571	28.54728177113869
2	11.932553057854216	9.402997312769784
3	4.603410948060609	5.441315940225054
4	2.2561478937366983	3.5557441025671523
5	1.3590898374343998	2.677448410924241
6	0.9661555680016751	2.284027106082944
7	0.7358313588336811	2.0294542916980514
8	0.5616707269852417	1.770413527062384
9	0.48243087455487066	1.7107263531695258
>10	4.528834719736511	35.072557566989424
>50	0.07778796310820815	2.0246002441673743
>100	0.037683939308386497	2.687025381191188
>500	0.0017286210691919474	0.5072737767188319
>1k	0.002765793710707116	2.2891342152953733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	3123	0.42244147636735496	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	2874	0.38875978324680704	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	2261	0.30584059496208443	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	2090	0.282709793662431	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	1969	0.2663423845556586	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1947	0.263366491990791	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	1524	0.20614819403901669	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1017	0.13756739720320207	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTG	866	0.11714195278070107	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	779	0.1053736503650879	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTT	760	0.10280356133179308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.058035315728674E-4	0.0	0.0	0.2208923890194975	0.0
2	4.058035315728674E-4	0.0	0.0	1.2156521127484532	0.0
3	5.410713754304899E-4	0.0	0.0	1.6996404580710265	0.0
4	5.410713754304899E-4	0.0	0.0	2.4338743145302013	0.0
5	5.410713754304899E-4	0.0	0.0	4.364822785597762	0.0
6	5.410713754304899E-4	0.0	0.0	5.78445880688351	0.0
7	6.763392192881124E-4	0.0	0.0	6.982120296398899	0.0
8	6.763392192881124E-4	0.0	0.0	9.089863839388373	0.0
9	6.763392192881124E-4	0.0	0.0	9.863054834878543	0.0
10	6.763392192881124E-4	0.0	0.0	11.800901965982842	0.0
11	6.763392192881124E-4	0.0	0.0	13.869553102097463	0.0
12	6.763392192881124E-4	0.0	0.0	15.921566293417596	0.0
13	6.763392192881124E-4	0.0	0.0	16.73560817775277	0.0
14	6.763392192881124E-4	0.0	0.0	17.079323768994985	0.0
15	6.763392192881124E-4	0.0	0.0	17.589824611713656	0.0
16	8.116070631457348E-4	0.0	0.0	18.6216477246596	0.0
17	8.116070631457348E-4	0.0	0.0	19.979736876990128	0.0
18	8.116070631457348E-4	0.0	0.0	21.467818427267833	0.0
19	8.116070631457348E-4	0.0	0.0	22.384393337247083	0.0
20	8.116070631457348E-4	0.0	0.0	23.24943119871658	0.0
21	0.0010821427508609799	0.0	0.0	24.287882436011547	0.0
22	0.0010821427508609799	0.0	0.0	25.337696172190554	0.0
23	0.0010821427508609799	0.0	0.0	26.514796949439585	0.0
24	0.0010821427508609799	0.0	0.0	27.338983922064077	0.0
25	0.0012174105947186022	0.0	0.0	28.122184737999714	0.0
26	0.0012174105947186022	0.0	0.0	28.85236055914316	0.0
27	0.0012174105947186022	0.0	0.0	29.578478344970875	0.0
28	0.0012174105947186022	0.0	0.0	30.353968893806627	0.0
29	0.0012174105947186022	0.0	0.0	31.123913461044214	0.0
30	0.0012174105947186022	0.0	0.0	31.960004003928177	0.0
31	0.0012174105947186022	0.0	0.0	32.76308919291088	0.0
32	0.0013526784385762248	0.0	0.0	33.50341010234365	0.0
33	0.0013526784385762248	0.0	0.0	34.28038859746184	0.0
34	0.0014879462824338473	0.0	0.0	35.050874236074854	0.0
35	0.0014879462824338473	0.0	0.0	35.77104023677283	0.0
36	0.0014879462824338473	0.0	0.0	36.48511918449722	0.0
37	0.0014879462824338473	0.0	0.0	37.18918831177615	0.0
38	0.0014879462824338473	0.0	0.0	37.87851324407459	0.0
39	0.0014879462824338473	0.0	0.0	38.62465067079324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCACG	20	7.031651E-4	45.000004	31
CGTATCA	20	7.031651E-4	45.000004	11
ACCGCTT	20	7.031651E-4	45.000004	16
CGCCTAA	20	7.031651E-4	45.000004	37
TCGGAGC	20	7.031651E-4	45.000004	40
TAGACCG	25	3.8894505E-5	45.0	1
AAGCGTA	25	3.8894505E-5	45.0	29
ACTACGG	25	3.8894505E-5	45.0	2
CGTACAA	25	3.8894505E-5	45.0	32
ACGGTGC	25	3.8894505E-5	45.0	27
TAGCGCG	30	2.1644464E-6	44.999996	1
TTCCGCG	30	2.1644464E-6	44.999996	1
TCGAGCG	30	2.1644464E-6	44.999996	1
GTCGAAG	65	0.0	41.538464	1
CGTTTTT	1550	0.0	41.225807	1
ATTTACG	45	1.9266736E-8	40.0	1
ATTACCG	45	1.9266736E-8	40.0	1
GCGGCTA	45	1.9266736E-8	40.0	24
CCGCGAA	45	1.9266736E-8	40.0	26
TATCCGG	45	1.9266736E-8	40.0	2
>>END_MODULE