SRR2935877.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935877.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574009
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	4946	0.8616589635354148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	3950	0.688142520413443	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	3283	0.5719422517765401	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3085	0.5374480191077143	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2559	0.4458118252501268	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1656	0.28849721868472444	RNA PCR Primer, Index 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	1295	0.225606218717825	RNA PCR Primer, Index 23 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	1223	0.21306286138370653	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	1192	0.2076622491981833	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	946	0.16480577830661192	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	828	0.14424860934236222	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	675	0.11759397500736052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	604	0.10522483096954925	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	580	0.10104371185817643	RNA PCR Primer, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAC	35	1.210501E-7	45.0	24
TCGGCTA	20	7.030334E-4	45.0	2
ATCCGGC	20	7.030334E-4	45.0	6
TGTTGCG	40	6.8048394E-9	45.0	1
GTCGTTG	20	7.030334E-4	45.0	1
GGCGCGA	20	7.030334E-4	45.0	8
GCTACGA	40	6.8048394E-9	45.0	10
CGCGTTA	25	3.8883565E-5	45.0	14
CGCCGGT	35	1.210501E-7	45.0	28
TAGCGCG	30	2.1635951E-6	44.999996	1
AGTTGCG	30	2.1635951E-6	44.999996	1
CGCTAGG	30	2.1635951E-6	44.999996	2
CGTTTTT	2430	0.0	43.24074	1
TAATACG	55	6.002665E-11	40.909092	4
TACGGCT	560	0.0	40.580357	7
CGTAAGG	95	0.0	40.263157	2
TACGCGG	45	1.9254003E-8	40.000004	2
CTTACGG	45	1.9254003E-8	40.000004	2
CGGAATG	40	3.454188E-7	39.375	6
CACGCCG	40	3.454188E-7	39.375	26