##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935877.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574009
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.759974146746828	31.0	31.0	34.0	30.0	34.0
2	31.81517537181473	33.0	31.0	34.0	30.0	34.0
3	31.809377553313624	33.0	31.0	34.0	30.0	34.0
4	35.554541827741375	37.0	35.0	37.0	33.0	37.0
5	33.49160204805151	37.0	35.0	37.0	30.0	37.0
6	34.47846113911106	37.0	35.0	37.0	30.0	37.0
7	35.597990623840396	37.0	35.0	37.0	32.0	37.0
8	35.615227287377024	37.0	35.0	37.0	33.0	37.0
9	37.501403288101756	39.0	37.0	39.0	35.0	39.0
10	36.8482427975868	39.0	37.0	39.0	32.0	39.0
11	36.95481778160273	39.0	37.0	39.0	33.0	39.0
12	37.04031992529734	39.0	37.0	39.0	33.0	39.0
13	37.06263490642133	39.0	37.0	39.0	33.0	39.0
14	38.16126576412565	40.0	37.0	41.0	33.0	41.0
15	38.22662362436826	40.0	37.0	41.0	33.0	41.0
16	38.22131186096385	40.0	37.0	41.0	34.0	41.0
17	38.19232276845833	40.0	37.0	41.0	34.0	41.0
18	38.1308533489893	40.0	37.0	41.0	34.0	41.0
19	37.98072155663065	40.0	37.0	41.0	34.0	41.0
20	37.962960511072126	40.0	36.0	41.0	34.0	41.0
21	37.911774902484105	40.0	36.0	41.0	33.0	41.0
22	37.99956446675923	40.0	36.0	41.0	34.0	41.0
23	38.006282131464836	40.0	37.0	41.0	34.0	41.0
24	37.97372689278391	40.0	36.0	41.0	34.0	41.0
25	37.764151781592275	39.0	36.0	41.0	33.0	41.0
26	37.86305789630476	40.0	36.0	41.0	34.0	41.0
27	37.83838406714877	40.0	36.0	41.0	33.0	41.0
28	37.686386450386664	40.0	36.0	41.0	33.0	41.0
29	37.67405214900812	40.0	36.0	41.0	33.0	41.0
30	37.525674684543276	40.0	36.0	41.0	33.0	41.0
31	37.399929269401696	40.0	36.0	41.0	33.0	41.0
32	37.32530848819444	40.0	36.0	41.0	32.0	41.0
33	37.17260356544932	40.0	36.0	41.0	32.0	41.0
34	36.97155967937785	40.0	36.0	41.0	31.0	41.0
35	36.900008536451516	40.0	36.0	41.0	31.0	41.0
36	36.78104872920111	40.0	36.0	41.0	31.0	41.0
37	36.75051610689031	40.0	36.0	41.0	31.0	41.0
38	36.625359532690254	39.0	35.0	41.0	30.0	41.0
39	36.57428019421298	39.0	35.0	41.0	30.0	41.0
40	36.55154361691193	39.0	35.0	41.0	30.0	41.0
41	36.39829514868234	39.0	35.0	41.0	30.0	41.0
42	36.45086052657711	39.0	35.0	41.0	30.0	41.0
43	36.391323132564125	39.0	35.0	41.0	30.0	41.0
44	36.25151173587871	39.0	35.0	41.0	29.0	41.0
45	36.213055892851855	39.0	35.0	41.0	29.0	41.0
46	36.08774078455216	39.0	35.0	41.0	28.0	41.0
47	35.99612549629013	39.0	35.0	41.0	28.0	41.0
48	35.922694591896644	39.0	35.0	40.0	28.0	41.0
49	35.827710018484034	39.0	35.0	40.0	28.0	41.0
50	35.70103256220721	39.0	35.0	40.0	27.0	41.0
51	34.35832190784465	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	9.0
12	5.0
13	6.0
14	10.0
15	9.0
16	26.0
17	63.0
18	145.0
19	296.0
20	579.0
21	996.0
22	1483.0
23	2129.0
24	3271.0
25	5016.0
26	6807.0
27	7284.0
28	7317.0
29	8095.0
30	9249.0
31	11623.0
32	15095.0
33	20521.0
34	31638.0
35	42231.0
36	44375.0
37	67820.0
38	117464.0
39	170358.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.930859969094556	19.63976174589597	23.73116100967058	13.698217275338889
2	32.346879578543195	26.105514025041423	24.51111393723792	17.03649245917747
3	29.104944347562494	25.964401255032588	29.10947389326648	15.821180504138438
4	26.187916914194727	24.471393305679875	31.718143792170505	17.622545987954894
5	23.66774736981476	32.69373825149083	26.254292180087766	17.38422219860664
6	22.047215287565177	36.19699342693233	27.7695994313678	13.986191854134692
7	77.10959235830796	4.8732685376013265	13.653096031595325	4.364043072495379
8	77.81550463494474	6.122203658827649	10.928748503943318	5.133543202284285
9	72.73736126088616	5.7326627282847475	13.093348710560287	8.43662730026881
10	35.61337888430321	23.99457151368707	23.44179272450432	16.950256877505403
11	26.352025839316106	25.915273105473958	27.657928708434888	20.07477234677505
12	21.765860814029047	21.71917165061872	34.196327932140434	22.318639603211796
13	22.180488459240184	24.930793768041966	36.09298808903693	16.795729683680918
14	20.247591936711792	28.90163743077199	31.853855949993815	18.9969146825224
15	17.82428498507863	27.30601784989434	36.87416050967842	17.99553665534861
16	20.4171014740187	25.94332144617941	31.743230506838742	21.89634657296314
17	20.7388734322981	26.491048049769255	30.20736608659446	22.562712431338184
18	21.78363057025238	25.42451424977657	32.34600851206166	20.445846667909386
19	20.558214244027535	28.58195603204828	29.666782228153217	21.193047495770973
20	21.007336121907496	27.509847406573762	31.545324202233765	19.937492269284977
21	20.414314061277786	29.577933447036546	31.44323521059774	18.564517281087927
22	18.904407422183276	25.472945546149973	31.060140172018208	24.562506859648543
23	17.82811767759739	28.3164549684761	30.820771102892113	23.03465625103439
24	19.603699593560382	25.684440487866915	31.714485312948053	22.99737460562465
25	18.364694630223568	29.620267278039194	29.485251973401116	22.529786118336123
26	18.753016067692318	27.521519697426346	31.393410207853883	22.33205402702745
27	20.82476058737755	27.41803699941987	29.291875214500124	22.465327198702457
28	18.813119654918303	26.74644474215561	32.08521120748978	22.355224395436306
29	22.22944239550251	24.434634300159054	30.00649815595226	23.329425148386175
30	21.93310557848396	26.464219202137944	29.114874505452004	22.487800713926088
31	21.03695238227972	29.220447763014167	26.657595961038936	23.08500389366717
32	23.471408984876543	28.295200946326627	27.48267013234984	20.75071993644699
33	22.709051600236233	26.927103930426178	26.78233268119489	23.581511788142695
34	22.017076387304034	28.914006574809804	27.64451428461923	21.424402753266936
35	21.259422761664016	26.775886789231528	28.763486286800383	23.201204162304077
36	22.59598717093286	29.666782228153217	26.96804405505837	20.769186545855554
37	19.655615155859927	30.076009261178832	28.997803170333565	21.27057241262768
38	21.11674207198842	29.325846807280026	27.761585619737673	21.79582550099389
39	23.361828821499316	28.126910902093872	27.554271797132103	20.956988479274717
40	22.889885001803105	26.008303005702	29.989076826321536	21.11273516617335
41	19.113637591048224	28.860174666250877	28.33283101832898	23.693356724371917
42	20.736260232853493	27.31298638174663	30.231407521484854	21.719345863915027
43	21.40924619648821	27.302882010560808	28.547984439268376	22.739887353682608
44	21.454890080120695	26.743831542711007	27.687196542214494	24.114081834953808
45	22.104008822161326	24.768252762587345	27.690158168251717	25.437580246999612
46	24.179411821069007	27.018914337580075	27.08389589710266	21.71777794424826
47	19.966760103064583	26.19366595297286	31.460482326932155	22.379091617030394
48	20.483128313319128	25.890360604102025	29.49117522547556	24.135335857103286
49	21.573877761498515	23.818093444527875	31.359961254962904	23.248067539010712
50	21.182420484696234	23.79109038360026	30.709971446440736	24.316517685262777
51	20.35055199482935	23.42559088794775	28.099385201277332	28.12447191594557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	272.5
2	401.0
3	3071.0
4	5741.0
5	3990.0
6	2239.0
7	2274.5
8	2310.0
9	2275.0
10	2240.0
11	2227.0
12	2214.0
13	2173.0
14	2132.0
15	2076.0
16	2020.0
17	1915.5
18	1811.0
19	1924.5
20	2038.0
21	1854.0
22	1670.0
23	2030.5
24	2391.0
25	2710.5
26	3470.5
27	3911.0
28	4962.0
29	6013.0
30	7238.0
31	8463.0
32	9612.0
33	10761.0
34	13001.5
35	15242.0
36	16862.5
37	18483.0
38	20325.5
39	22168.0
40	24796.5
41	27425.0
42	31244.5
43	35064.0
44	39811.0
45	44558.0
46	59761.0
47	74964.0
48	72538.0
49	70112.0
50	68059.5
51	66007.0
52	55777.5
53	45548.0
54	38736.5
55	31925.0
56	26811.5
57	21698.0
58	18560.5
59	15423.0
60	12952.5
61	10482.0
62	8673.5
63	6865.0
64	5448.5
65	4032.0
66	3095.0
67	2158.0
68	1598.0
69	1038.0
70	835.0
71	632.0
72	461.0
73	290.0
74	244.5
75	133.5
76	68.0
77	52.0
78	36.0
79	31.5
80	27.0
81	17.5
82	8.0
83	11.0
84	14.0
85	9.0
86	4.0
87	4.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	574009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.92641628477684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41393341992516	33.12663833076077
2	10.532322671732487	9.252943802482285
3	3.939145144532245	5.190975882746426
4	2.097512542653245	3.6854483644450866
5	1.3116879738522231	2.8808875987584117
6	0.9919786847501447	2.6144441191176164
7	0.7836344023306673	2.409557568029443
8	0.6406866010168248	2.251445307547502
9	0.5141718427660248	2.03271537665261
>10	3.6913738922383366	28.278608663153467
>50	0.05396952762970694	1.5801074112698648
>100	0.02278713384669848	1.7147976049048437
>500	0.003198194224098032	0.9092905272091429
>1k	0.003597968502110286	4.072139442922521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	4946	0.8616589635354148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	3950	0.688142520413443	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	3283	0.5719422517765401	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3085	0.5374480191077143	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2559	0.4458118252501268	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1656	0.28849721868472444	RNA PCR Primer, Index 1 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	1295	0.225606218717825	RNA PCR Primer, Index 23 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	1223	0.21306286138370653	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	1192	0.2076622491981833	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	946	0.16480577830661192	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	828	0.14424860934236222	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	675	0.11759397500736052	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	604	0.10522483096954925	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	580	0.10104371185817643	RNA PCR Primer, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7421329630720075E-4	0.0	0.0	0.22229616608798816	0.0
2	1.7421329630720075E-4	0.0	0.0	1.1877862542224948	0.0
3	1.7421329630720075E-4	0.0	0.0	1.6748866306974282	0.0
4	1.7421329630720075E-4	0.0	0.0	2.4508326524497	0.0
5	1.7421329630720075E-4	0.0	0.0	4.678323859033569	0.0
6	1.7421329630720075E-4	0.0	0.0	6.140496054939905	0.0
7	1.7421329630720075E-4	0.0	0.0	7.31399681886521	0.0
8	1.7421329630720075E-4	0.0	0.0	9.141668510424052	0.0
9	1.7421329630720075E-4	0.0	0.0	9.78468978709393	0.0
10	1.7421329630720075E-4	0.0	0.0	11.822288500702951	0.0
11	1.7421329630720075E-4	0.0	0.0	13.621040785074799	0.0
12	1.7421329630720075E-4	0.0	0.0	15.775188193913335	0.0
13	3.484265926144015E-4	0.0	0.0	16.49259854810639	0.0
14	3.484265926144015E-4	0.0	0.0	16.805834054866736	0.0
15	3.484265926144015E-4	0.0	0.0	17.30408408230533	0.0
16	3.484265926144015E-4	0.0	0.0	18.256682386513102	0.0
17	3.484265926144015E-4	0.0	0.0	19.4517855991805	0.0
18	3.484265926144015E-4	0.0	0.0	20.754726842262055	0.0
19	3.484265926144015E-4	0.0	0.0	21.627883883353746	0.0
20	3.484265926144015E-4	0.0	0.0	22.375955777696866	0.0
21	3.484265926144015E-4	0.0	0.0	23.291098223198592	0.0
22	3.484265926144015E-4	0.0	0.0	24.262511563407543	0.0
23	3.484265926144015E-4	0.0	0.0	25.263192737396103	0.0
24	5.226398889216023E-4	0.0	0.0	26.023459562480728	0.0
25	5.226398889216023E-4	0.0	0.0	26.79574710501055	0.0
26	5.226398889216023E-4	0.0	0.0	27.468210428756343	0.0
27	5.226398889216023E-4	0.0	0.0	28.147642284354426	0.0
28	5.226398889216023E-4	0.0	0.0	28.832823178730646	0.0
29	5.226398889216023E-4	0.0	0.0	29.51295188751396	0.0
30	6.96853185228803E-4	0.0	0.0	30.3050997458228	0.0
31	6.96853185228803E-4	0.0	0.0	31.031917618016443	0.0
32	6.96853185228803E-4	0.0	0.0	31.703858301873314	0.0
33	8.710664815360038E-4	0.0	0.0	32.389039196249534	0.0
34	8.710664815360038E-4	0.0	0.0	33.07387166403314	0.0
35	0.0010452797778432046	0.0	0.0	33.72978472462975	0.0
36	0.0010452797778432046	0.0	0.0	34.358346297706134	0.0
37	0.0012194930741504053	0.0	0.0	35.00781346633938	0.0
38	0.0012194930741504053	0.0	0.0	35.673308258232886	0.0
39	0.0012194930741504053	0.0	0.0	36.308315723272635	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAC	35	1.210501E-7	45.0	24
TCGGCTA	20	7.030334E-4	45.0	2
ATCCGGC	20	7.030334E-4	45.0	6
TGTTGCG	40	6.8048394E-9	45.0	1
GTCGTTG	20	7.030334E-4	45.0	1
GGCGCGA	20	7.030334E-4	45.0	8
GCTACGA	40	6.8048394E-9	45.0	10
CGCGTTA	25	3.8883565E-5	45.0	14
CGCCGGT	35	1.210501E-7	45.0	28
TAGCGCG	30	2.1635951E-6	44.999996	1
AGTTGCG	30	2.1635951E-6	44.999996	1
CGCTAGG	30	2.1635951E-6	44.999996	2
CGTTTTT	2430	0.0	43.24074	1
TAATACG	55	6.002665E-11	40.909092	4
TACGGCT	560	0.0	40.580357	7
CGTAAGG	95	0.0	40.263157	2
TACGCGG	45	1.9254003E-8	40.000004	2
CTTACGG	45	1.9254003E-8	40.000004	2
CGGAATG	40	3.454188E-7	39.375	6
CACGCCG	40	3.454188E-7	39.375	26
>>END_MODULE