##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935876.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	915408
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.059431422928355	33.0	31.0	34.0	30.0	34.0
2	32.116047707688814	33.0	31.0	34.0	30.0	34.0
3	32.11550368797301	33.0	31.0	34.0	30.0	34.0
4	35.81687837554402	37.0	35.0	37.0	35.0	37.0
5	33.65421211088389	37.0	35.0	37.0	32.0	37.0
6	34.67181409819447	37.0	35.0	37.0	31.0	37.0
7	35.79579706535228	37.0	35.0	37.0	33.0	37.0
8	35.8491437697726	37.0	35.0	37.0	35.0	37.0
9	37.77503801583556	39.0	38.0	39.0	35.0	39.0
10	37.29791415412581	39.0	37.0	39.0	34.0	39.0
11	37.26398065125059	39.0	37.0	39.0	34.0	39.0
12	37.31989997902575	39.0	37.0	39.0	34.0	39.0
13	37.332211429220635	39.0	37.0	39.0	34.0	39.0
14	38.455214505444566	40.0	38.0	41.0	34.0	41.0
15	38.554669611801515	40.0	38.0	41.0	34.0	41.0
16	38.55301570447276	40.0	38.0	41.0	34.0	41.0
17	38.51940446227256	40.0	38.0	41.0	34.0	41.0
18	38.46442460629577	40.0	38.0	41.0	34.0	41.0
19	38.35121279254715	40.0	37.0	41.0	34.0	41.0
20	38.35729204900984	40.0	37.0	41.0	34.0	41.0
21	38.28066610735323	40.0	37.0	41.0	34.0	41.0
22	38.356977435198296	40.0	37.0	41.0	34.0	41.0
23	38.347803383846326	40.0	37.0	41.0	34.0	41.0
24	38.29525304563648	40.0	37.0	41.0	34.0	41.0
25	38.13484806774684	40.0	37.0	41.0	34.0	41.0
26	38.19152661982417	40.0	37.0	41.0	34.0	41.0
27	38.18942482477758	40.0	37.0	41.0	34.0	41.0
28	38.11177529582437	40.0	37.0	41.0	34.0	41.0
29	38.13055162288291	40.0	37.0	41.0	34.0	41.0
30	37.99063805428836	40.0	37.0	41.0	34.0	41.0
31	37.92599037806093	40.0	37.0	41.0	34.0	41.0
32	37.80899336689214	40.0	37.0	41.0	33.0	41.0
33	37.70516206980931	40.0	37.0	41.0	33.0	41.0
34	37.546726705469034	40.0	37.0	41.0	33.0	41.0
35	37.45398554524321	40.0	37.0	41.0	33.0	41.0
36	37.35844781780364	40.0	36.0	41.0	33.0	41.0
37	37.333126868019505	40.0	36.0	41.0	33.0	41.0
38	37.20822409242655	40.0	36.0	41.0	32.0	41.0
39	37.17506292276231	40.0	36.0	41.0	32.0	41.0
40	37.0698267876182	40.0	36.0	41.0	32.0	41.0
41	36.978336435775084	40.0	36.0	41.0	31.0	41.0
42	36.99308614300946	40.0	36.0	41.0	31.0	41.0
43	36.952771878768814	40.0	35.0	41.0	31.0	41.0
44	36.84496312026987	40.0	35.0	41.0	31.0	41.0
45	36.76950059427053	39.0	35.0	41.0	31.0	41.0
46	36.674640160453045	39.0	35.0	41.0	31.0	41.0
47	36.60146513904183	39.0	35.0	41.0	31.0	41.0
48	36.51592950902767	39.0	35.0	41.0	31.0	41.0
49	36.441780058727915	39.0	35.0	41.0	30.0	41.0
50	36.345673186163985	39.0	35.0	41.0	30.0	41.0
51	35.186616241064094	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	24.0
10	14.0
11	11.0
12	13.0
13	18.0
14	19.0
15	38.0
16	42.0
17	81.0
18	193.0
19	362.0
20	706.0
21	1215.0
22	1847.0
23	2580.0
24	3664.0
25	5724.0
26	7883.0
27	9028.0
28	9572.0
29	10299.0
30	11896.0
31	15190.0
32	19737.0
33	28287.0
34	47551.0
35	60623.0
36	65625.0
37	102920.0
38	185321.0
39	324714.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24344991522906	19.232517085277824	23.405301242724555	14.118731756768565
2	32.973493786377226	24.9064897837904	25.345092024539877	16.774924405292506
3	31.128961075280092	25.275396326009826	27.457483439078533	16.138159159631552
4	27.266639574921786	25.35022634715886	28.904051526750912	18.479082551168442
5	24.303807701046964	33.336829042350516	24.67107562966459	17.688287626937935
6	24.71925086955762	35.09396902801811	25.391300928110745	14.795479174313531
7	80.7548109695349	4.8360949434569065	10.586863999440686	3.822230087567511
8	82.48496845122611	5.365367136839529	8.304275252128013	3.845389159806338
9	76.60103472986908	6.724542499082377	10.104019191442504	6.570403579606034
10	34.567209375491586	34.86008424658731	16.832603604075995	13.740102773845106
11	25.750157306905773	26.00414241518536	30.03524111652946	18.210459161379408
12	23.696755982032055	23.85187806966948	31.61803261496513	20.833333333333336
13	23.553431912327618	24.35263838638072	33.90215073497282	18.191778966318843
14	20.222676664394456	27.77231573243843	31.105365039414117	20.899642563752995
15	18.497653505322216	26.719342631919318	34.94900634471186	19.833997518046598
16	22.235658853757013	27.215514830545505	30.759071364899587	19.789754950797896
17	22.4428888539318	26.030906437348158	30.275898834181042	21.250305874539002
18	23.155467288902873	24.910094733714367	31.246395050076032	20.688042927306732
19	22.116804747172843	27.43028245328859	28.989150193137924	21.463762606400643
20	23.340630625906698	27.216388757799802	30.223681680736895	19.2192989355566
21	22.263296803174104	28.748492475486344	30.097508433398	18.89070228794155
22	22.288094489014735	24.531574991697692	30.480397811686156	22.69993270760142
23	20.29510338559418	27.850095258070716	30.80386013668222	21.050941219652877
24	20.241575341268593	26.665159142153005	30.9026139164176	22.190651600160802
25	20.502224144862183	29.36941778966319	29.294041563980215	20.834316501494417
26	20.91176830440634	27.094148183105236	30.062332861412617	21.931750651075806
27	21.04580689703389	27.163079195287782	30.235698180483457	21.55541572719487
28	18.557736004055023	27.770895600650203	31.727273521752046	21.944094873542728
29	20.430125146382817	25.691385699054408	31.23295841854124	22.64553073602153
30	21.6294810619964	25.965580375089576	31.658451750476292	20.746486812437734
31	22.490627130197684	26.997360739692027	28.83828850086519	21.6737236292451
32	22.003631167741595	27.517019733277404	28.949386503067487	21.529962595913517
33	22.43360337685491	26.04456155069652	28.39356876933564	23.12826630311293
34	21.585347735654484	27.33087322811249	29.36144320346774	21.72233583276528
35	20.469561113732894	27.08486270602835	29.755802876968524	22.689773303270236
36	21.954909723314632	28.318192543652664	28.23309387726566	21.493803855767048
37	21.10436002307168	27.41968608533026	29.214623424746122	22.261330466851938
38	20.951422753569993	29.135205285512033	27.56290091412791	22.350471046790066
39	22.70703336654257	25.48710520336287	27.757349728208624	24.048511701885936
40	23.763174453358502	25.924615035044486	28.96478947092444	21.34742104067257
41	20.751948857777077	26.76183734465943	28.463155226958907	24.023058570604583
42	21.268658346879203	26.733325467988045	29.45735671962666	22.540659465506092
43	21.415041161973676	25.644412109136034	29.5455141314037	23.395032597486583
44	21.8808443885131	26.29559715449286	29.100794399874154	22.722764057119885
45	21.420503207313025	25.242624053973746	28.560161152185692	24.776711586527536
46	22.9689930610176	26.284017618373447	28.14329785188681	22.603691468722143
47	20.62632181497212	26.451374687571004	30.656166430706307	22.266137066750563
48	21.055201615017566	26.47300438711482	29.19605247059235	23.27574152727527
49	21.51357645989548	25.249833953821682	30.07926520196459	23.157324384318247
50	20.94650691276458	25.559422683655814	30.217236467236468	23.276833936343138
51	20.763091430269345	24.51497037386608	28.6954013947879	26.026536801076677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	550.0
2	911.0
3	4682.0
4	8453.0
5	5794.5
6	3136.0
7	3032.0
8	2928.0
9	2926.5
10	2925.0
11	2936.5
12	2948.0
13	2908.5
14	2869.0
15	2765.0
16	2661.0
17	2695.0
18	2729.0
19	2645.0
20	2561.0
21	2834.5
22	3108.0
23	3772.5
24	4437.0
25	5244.5
26	6991.5
27	7931.0
28	10418.5
29	12906.0
30	14783.5
31	16661.0
32	19208.0
33	21755.0
34	25289.5
35	28824.0
36	31447.5
37	34071.0
38	36813.0
39	39555.0
40	43210.5
41	46866.0
42	51212.0
43	55558.0
44	60392.5
45	65227.0
46	78866.0
47	92505.0
48	91617.5
49	90730.0
50	88101.5
51	85473.0
52	77231.0
53	68989.0
54	60529.5
55	52070.0
56	46445.0
57	40820.0
58	36323.5
59	31827.0
60	28204.5
61	24582.0
62	21164.5
63	17747.0
64	15147.0
65	12547.0
66	10379.0
67	8211.0
68	6811.5
69	5412.0
70	4169.0
71	2926.0
72	2723.5
73	2521.0
74	2131.5
75	1435.0
76	1128.0
77	849.5
78	571.0
79	344.5
80	118.0
81	133.5
82	149.0
83	96.5
84	44.0
85	27.0
86	10.0
87	8.0
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	8.0
94	14.0
95	7.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	915408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.488222015814138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.23945538798327	22.746920095583185
2	9.939686229161298	6.259660935027228
3	3.6915988013249184	3.487256479482971
4	1.9833205536520324	2.498049516876905
5	1.3170268008915769	2.0735416153625708
6	0.9634224858207002	1.8201876677129853
7	0.7990634986759837	1.7612762195729808
8	0.6939742144832386	1.748161131111875
9	0.619705746988755	1.7562088931092075
>10	7.6140380455512995	49.49222685240434
>50	0.1050820106924892	2.077293140362741
>100	0.02872240032823698	1.5211858115677428
>500	0.003152458572610221	0.7503354898559318
>1k	0.0017513658736723447	2.007696151969356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4600	0.5025081712198277	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	4117	0.44974481324174576	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	4110	0.44898012689423733	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	3594	0.3926118189921871	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1782	0.19466729589428974	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	996	0.10880394315977138	TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	980	0.10705608865118069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.554454407215143E-4	0.0	0.0	0.08586335273451838	0.0
2	6.554454407215143E-4	0.0	0.0	0.4869959624560852	0.0
3	6.554454407215143E-4	0.0	0.0	0.7458969115410833	0.0
4	6.554454407215143E-4	0.0	0.0	1.1117447083704752	0.0
5	6.554454407215143E-4	0.0	0.0	2.3082603604075995	0.0
6	6.554454407215143E-4	0.0	0.0	3.1696249104224563	0.0
7	6.554454407215143E-4	0.0	0.0	3.9268828762693793	0.0
8	6.554454407215143E-4	0.0	0.0	5.171355286385961	0.0
9	6.554454407215143E-4	0.0	0.0	5.727391501931379	0.0
10	7.646863475084334E-4	0.0	0.0	7.079466205233077	0.0
11	7.646863475084334E-4	0.0	0.0	8.059247898204953	0.0
12	7.646863475084334E-4	0.0	0.0	9.397121283624351	0.0
13	8.739272542953525E-4	0.0	0.0	9.81453078845717	0.0
14	8.739272542953525E-4	0.0	0.0	10.019357488682642	0.0
15	8.739272542953525E-4	0.0	0.0	10.321627077762047	0.0
16	8.739272542953525E-4	0.0	0.0	10.789287399716848	0.0
17	8.739272542953525E-4	0.0	0.0	11.41392690472445	0.0
18	8.739272542953525E-4	0.0	0.0	12.081498086099312	0.0
19	8.739272542953525E-4	0.0	0.0	12.554729694300246	0.0
20	9.831681610822714E-4	0.0	0.0	12.99999563036373	0.0
21	9.831681610822714E-4	0.0	0.0	13.493983010854176	0.0
22	9.831681610822714E-4	0.0	0.0	14.044775662873823	0.0
23	9.831681610822714E-4	0.0	0.0	14.580820792477235	0.0
24	9.831681610822714E-4	0.0	0.0	14.984684424868474	0.0
25	9.831681610822714E-4	0.0	0.0	15.36069162602905	0.0
26	0.0010924090678691907	0.0	0.0	15.722060545680177	0.0
27	0.0010924090678691907	0.0	0.0	16.134335727894012	0.0
28	0.0012016499746561097	0.0	0.0	16.52651058325905	0.0
29	0.0013108908814430287	0.0	0.0	16.949709856151575	0.0
30	0.0013108908814430287	0.0	0.0	17.46936884973695	0.0
31	0.0015293726950168667	0.0	0.0	17.913760858546134	0.0
32	0.0015293726950168667	0.0	0.0	18.359026794609616	0.0
33	0.0015293726950168667	0.0	0.0	18.77621781762886	0.0
34	0.0016386136018037858	0.0	0.0	19.192971877020955	0.0
35	0.0016386136018037858	0.0	0.0	19.651455962805656	0.0
36	0.0016386136018037858	0.0	0.0	20.077604740181428	0.0
37	0.0016386136018037858	0.0	0.0	20.49840071312464	0.0
38	0.0016386136018037858	0.0	0.0	20.962674566969046	0.0
39	0.0016386136018037858	0.0	0.0	21.495879432996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAG	30	2.1650158E-6	45.000004	1
TAGTGCG	30	2.1650158E-6	45.000004	1
TAGTACG	30	2.1650158E-6	45.000004	1
CGACGGT	20	7.0325326E-4	45.0	28
AATGCGC	20	7.0325326E-4	45.0	17
CAATGCG	35	1.2115743E-7	45.0	1
TAGGCCG	20	7.0325326E-4	45.0	1
CGTTTTT	2520	0.0	42.053574	1
CGAATAT	110	0.0	40.909092	14
CGTTATT	225	0.0	40.0	1
ACGGGCG	165	0.0	39.545452	5
AGTAACG	40	3.4571895E-7	39.375	1
AATGCGG	110	0.0	38.863636	2
TATGCGG	105	0.0	38.57143	2
CGTAAGG	210	0.0	38.57143	2
CCGTAAG	30	1.1397403E-4	37.500004	1
TACGAAT	120	0.0	37.500004	12
TTACGGG	325	0.0	37.384617	3
AGTACGG	145	0.0	37.24138	2
TCGATGG	85	0.0	37.058823	2
>>END_MODULE