SRR2935875.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589798
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	14025	2.3779327837666457	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	12907	2.188376359363714	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	11639	1.9733874987707658	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	5494	0.9315053628530446	TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	3100	0.5256036812603637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	2823	0.4786384490961312	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	2357	0.39962834733247654	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	2341	0.39691555413887464	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	2114	0.358427800704648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.33418221153683125	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1932	0.32756977812742666	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1622	0.27500941000139034	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT	1475	0.250085622535173	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	972	0.16480218651131404	TruSeq Adapter, Index 20 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	727	0.1232625407342853	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	718	0.12173659456288424	No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	694	0.11766740477248143	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG	672	0.11393731413127885	No Hit
GAATATGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	671	0.11376776455667872	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG	632	0.10715533114727416	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	592	0.10037334816326945	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.0304895E-4	45.0	4
CGGGTCG	35	1.2105738E-7	45.0	6
CCTAGTG	20	7.0304895E-4	45.0	29
TTGTACG	20	7.0304895E-4	45.0	1
CGATTCC	20	7.0304895E-4	45.0	21
CTAAGCG	25	3.8884886E-5	45.0	1
TCGATTC	20	7.0304895E-4	45.0	20
TGGCCGT	20	7.0304895E-4	45.0	33
ACCTCGA	20	7.0304895E-4	45.0	19
GTGCTCG	20	7.0304895E-4	45.0	33
CGGATCC	40	6.8048394E-9	45.0	6
TACCTCG	20	7.0304895E-4	45.0	18
TGTTACG	20	7.0304895E-4	45.0	1
AAATTCG	20	7.0304895E-4	45.0	33
TCGTACA	20	7.0304895E-4	45.0	37
CCCCACG	20	7.0304895E-4	45.0	38
GTATACG	25	3.8884886E-5	45.0	1
ATGCGCG	20	7.0304895E-4	45.0	1
TTACGGT	20	7.0304895E-4	45.0	1
CGCACTT	35	1.2105738E-7	45.0	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content