##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589798
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.581711026487035	31.0	31.0	34.0	30.0	34.0
2	31.62491395359089	31.0	31.0	34.0	30.0	34.0
3	31.663762847619015	31.0	31.0	34.0	30.0	34.0
4	35.4529364290825	37.0	35.0	37.0	33.0	37.0
5	33.320236759025974	37.0	35.0	37.0	30.0	37.0
6	34.291923675563496	37.0	35.0	37.0	30.0	37.0
7	35.42763624156067	37.0	35.0	37.0	32.0	37.0
8	35.34800050186674	37.0	35.0	37.0	33.0	37.0
9	37.20275585878555	39.0	37.0	39.0	34.0	39.0
10	36.71727777985005	39.0	35.0	39.0	32.0	39.0
11	36.78201689391961	39.0	35.0	39.0	32.0	39.0
12	36.79979755780793	39.0	35.0	39.0	33.0	39.0
13	36.70075517380527	39.0	35.0	39.0	32.0	39.0
14	37.699770090776845	39.0	36.0	41.0	33.0	41.0
15	37.698951166331526	39.0	36.0	41.0	33.0	41.0
16	37.837188325494495	39.0	36.0	41.0	33.0	41.0
17	37.70266938850251	39.0	36.0	41.0	33.0	41.0
18	37.69757951027301	39.0	36.0	41.0	33.0	41.0
19	37.600461513942065	39.0	36.0	41.0	33.0	41.0
20	37.50346050681759	39.0	36.0	41.0	32.0	41.0
21	37.569895116633155	39.0	36.0	41.0	33.0	41.0
22	37.65611446630881	39.0	36.0	41.0	33.0	41.0
23	37.64173835787846	39.0	36.0	41.0	33.0	41.0
24	37.57795380791389	39.0	36.0	41.0	33.0	41.0
25	37.46066449869277	39.0	35.0	41.0	33.0	41.0
26	37.455557665505815	39.0	36.0	41.0	33.0	41.0
27	37.48085107104466	39.0	36.0	41.0	33.0	41.0
28	37.35558614983435	39.0	36.0	41.0	32.0	41.0
29	37.34086246477607	39.0	36.0	41.0	32.0	41.0
30	37.277620473450234	39.0	36.0	41.0	32.0	41.0
31	37.21966842885191	39.0	35.0	41.0	32.0	41.0
32	37.11482575390218	39.0	35.0	41.0	32.0	41.0
33	37.013731820046864	39.0	35.0	41.0	31.0	41.0
34	36.872081627947196	39.0	35.0	41.0	31.0	41.0
35	36.874150132757315	39.0	35.0	41.0	31.0	41.0
36	36.73194381805296	39.0	35.0	41.0	31.0	41.0
37	36.7450923875632	39.0	35.0	41.0	31.0	41.0
38	36.60716550412175	39.0	35.0	41.0	30.0	41.0
39	36.58835058782837	39.0	35.0	41.0	30.0	41.0
40	36.5588726987884	39.0	35.0	41.0	30.0	41.0
41	36.324555186691036	39.0	35.0	41.0	30.0	41.0
42	36.43032021132659	39.0	35.0	41.0	30.0	41.0
43	36.367464793030834	39.0	35.0	40.0	30.0	41.0
44	36.27654383365152	39.0	35.0	40.0	30.0	41.0
45	36.27336308363202	39.0	35.0	40.0	30.0	41.0
46	36.143686482490615	39.0	35.0	40.0	30.0	41.0
47	36.02855553935415	39.0	35.0	40.0	30.0	41.0
48	35.96706669062967	38.0	35.0	40.0	29.0	41.0
49	35.944874346810266	39.0	35.0	40.0	29.0	41.0
50	35.76636407719253	38.0	35.0	40.0	28.0	41.0
51	34.4699609018681	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	7.0
11	6.0
12	5.0
13	3.0
14	13.0
15	9.0
16	31.0
17	55.0
18	136.0
19	313.0
20	708.0
21	1180.0
22	1943.0
23	2701.0
24	3462.0
25	4490.0
26	5562.0
27	6199.0
28	6965.0
29	8179.0
30	10139.0
31	13333.0
32	18223.0
33	25321.0
34	39335.0
35	50106.0
36	49454.0
37	72030.0
38	118512.0
39	151332.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.224476854787575	18.46801786374318	21.955652613267592	11.351852668201655
2	28.52468133157454	31.613535481639477	23.257623796621893	16.60415939016409
3	26.27747126982458	32.477899212950874	26.122842057789274	15.121787459435264
4	24.728127257128712	22.883597435054035	35.523179122343585	16.86509618547367
5	28.530446017110943	29.842081526217452	23.83087090834489	17.796601548326716
6	21.374945320262192	38.71918860355579	25.753732633884823	14.152133442297194
7	70.80068091109159	5.005612090919264	19.151811298105454	5.041895699883689
8	68.96818910881353	11.283354640063207	12.316928846825522	7.431527404297743
9	63.919172326796634	5.671263720799324	14.174344436569807	16.235219515834235
10	39.991997260078875	17.81389560493593	23.56874726601311	18.625359868972087
11	30.603020017022775	22.20862057857097	26.501615807445937	20.686743596960316
12	24.459221631812923	18.91579829026209	32.82038935364311	23.804590724281873
13	23.246433524698286	21.779490605258072	37.738683413643315	17.235392456400326
14	19.460900172601466	29.29596234643047	29.483992824662003	21.759144656306056
15	16.698089854492554	23.027884123038735	40.59779789012509	19.676228132343613
16	18.82983665593983	24.084347522372067	30.53909982739853	26.54671599428957
17	18.867985310224856	25.613515135690523	31.59064628906846	23.927853265016157
18	19.694539486400426	24.31951278234243	32.57505111919674	23.4108966120604
19	18.656726540273112	28.060793695468618	28.723393433005878	24.559086331252395
20	20.336454175836476	26.96245155120906	33.45196151902855	19.24913275392592
21	19.424955662786243	31.22899704644641	30.092167148752623	19.253880142014722
22	17.017182153889976	24.541961824217783	31.064533959084294	27.376322062807944
23	17.35865499713461	29.359204337756317	29.814444945557632	23.46769571955144
24	20.715906123791537	24.806798259743164	28.45380960939169	26.02348600707361
25	16.282862946296866	32.55165327790193	27.073167423422934	24.09231635237827
26	16.826099783315644	26.559432212384582	31.398207521897326	25.216260482402447
27	21.139101861993428	26.744241248698707	27.172523474138604	24.94413341516926
28	15.458173815441898	27.942278542823136	31.92771084337349	24.671836798361475
29	18.51210075313921	25.871400038657306	27.742549143944196	27.87395006425929
30	18.012438156792665	28.67117216402904	27.08130580300374	26.235083876174553
31	19.184703915577874	28.874631653549184	24.717106534779703	27.223557896093237
32	19.76625895645628	29.08928141499293	24.788486905686355	26.35597272286444
33	18.578055537658656	23.493467254890657	24.763224019070936	33.16525318837975
34	17.920711836934	27.187952485427214	30.060800477451604	24.83053520018718
35	16.70673688279716	23.85901613772851	28.594196657160587	30.84005032231374
36	21.107904740267006	28.99619869853747	26.021281862603807	23.87461469859172
37	16.768792027100805	24.48228037395854	31.541985561158224	27.20694203778243
38	17.6262042258536	27.132679324107578	25.310190946730916	29.930925503307915
39	21.168433938399247	22.70234893980651	29.296640544728874	26.83257657706537
40	18.0595729385315	21.73133852607164	32.76206429998067	27.447024235416194
41	19.184703915577874	24.662172472609267	29.829026208973243	26.324097402839612
42	19.519564325413107	22.75863939857375	33.812254365053796	23.909541910959344
43	24.254914394419785	22.736597953875734	26.42701399462189	26.581473657082594
44	19.43987602535105	23.290685963668917	28.225256782830733	29.044181228149295
45	17.86459092774136	21.744054744166647	28.051298919291014	32.34005540880098
46	24.257457638038787	24.15962753349452	27.272557723152673	24.310357105314022
47	16.445121889189178	22.168776428539942	36.37024879704577	25.015852885225108
48	19.543131716282524	23.360031739680366	28.654217206569026	28.442619337468084
49	19.618920376128777	18.606709415766076	35.5419991251242	26.232371082980954
50	20.581114211984442	19.011085151187356	31.621165212496482	28.78663542433172
51	18.59687554043927	18.936652887937903	27.795279061644834	34.67119250997799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	290.5
2	410.0
3	2121.0
4	3832.0
5	2639.5
6	1447.0
7	1446.5
8	1446.0
9	1529.0
10	1612.0
11	1597.0
12	1582.0
13	1510.0
14	1438.0
15	1427.0
16	1416.0
17	1421.5
18	1427.0
19	1450.0
20	1473.0
21	1566.5
22	1660.0
23	1778.5
24	1897.0
25	2209.5
26	2876.0
27	3230.0
28	3595.0
29	3960.0
30	4711.5
31	5463.0
32	6440.0
33	7417.0
34	8762.0
35	10107.0
36	11607.0
37	13107.0
38	14964.0
39	16821.0
40	19301.0
41	21781.0
42	25871.5
43	29962.0
44	36999.5
45	44037.0
46	77285.5
47	110534.0
48	94607.5
49	78681.0
50	76664.0
51	74647.0
52	60341.0
53	46035.0
54	38491.0
55	30947.0
56	26351.0
57	21755.0
58	18998.5
59	16242.0
60	14026.5
61	11811.0
62	10393.5
63	8976.0
64	7075.0
65	5174.0
66	4129.0
67	3084.0
68	2390.0
69	1696.0
70	1300.0
71	904.0
72	712.0
73	520.0
74	388.0
75	188.5
76	121.0
77	108.5
78	96.0
79	73.5
80	51.0
81	40.5
82	30.0
83	18.5
84	7.0
85	6.0
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	589798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.008762561711706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32991054971718	29.738477424169048
2	11.79012754917865	9.43416827374769
3	4.286226029180519	5.144597984619354
4	2.1422832885931964	3.4284041373299248
5	1.3169440611008307	2.6344651123819744
6	0.8956995325582202	2.1501497954854796
7	0.6649301763393423	1.8622123481694484
8	0.5584446860154638	1.7874144677313872
9	0.44517985264434545	1.602998551953488
>10	3.4471923936771365	25.520323060963563
>50	0.0751101825481199	2.0264667413765647
>100	0.03819161823212178	2.679601589383095
>500	0.004243513136902419	1.158902645196034
>1k	0.0038191618232121774	3.35056309741338
>5k	4.243513136902419E-4	0.9327587361129522
>10k+	0.0012730539410707258	6.548496033966633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	14025	2.3779327837666457	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	12907	2.188376359363714	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	11639	1.9733874987707658	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	5494	0.9315053628530446	TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	3100	0.5256036812603637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	2823	0.4786384490961312	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	2357	0.39962834733247654	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	2341	0.39691555413887464	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	2114	0.358427800704648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.33418221153683125	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1932	0.32756977812742666	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1622	0.27500941000139034	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT	1475	0.250085622535173	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	972	0.16480218651131404	TruSeq Adapter, Index 20 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	727	0.1232625407342853	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	718	0.12173659456288424	No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	694	0.11766740477248143	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG	672	0.11393731413127885	No Hit
GAATATGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	671	0.11376776455667872	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG	632	0.10715533114727416	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	592	0.10037334816326945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.781982984004693E-4	0.0	0.0	0.294846710229604	0.0
2	6.781982984004693E-4	0.0	0.0	1.9457509181109465	0.0
3	6.781982984004693E-4	0.0	0.0	2.506112262164334	0.0
4	6.781982984004693E-4	0.0	0.0	3.761457312503603	0.0
5	6.781982984004693E-4	0.0	0.0	7.763166372215572	0.0
6	6.781982984004693E-4	0.0	0.0	9.382025710497492	0.0
7	8.477478730005867E-4	0.0	0.0	11.139237501653108	0.0
8	8.477478730005867E-4	0.0	0.0	13.726055361327099	0.0
9	8.477478730005867E-4	0.0	0.0	14.544301608347265	0.0
10	8.477478730005867E-4	0.0	0.0	18.420035334131345	0.0
11	8.477478730005867E-4	0.0	0.0	20.872739480296644	0.0
12	8.477478730005867E-4	0.0	0.0	25.209817598567646	0.0
13	0.001017297447600704	0.0	0.0	26.14217070929369	0.0
14	0.001017297447600704	0.0	0.0	26.569266087711387	0.0
15	0.001017297447600704	0.0	0.0	27.50212784716123	0.0
16	0.001017297447600704	0.0	0.0	28.806981373283733	0.0
17	0.001017297447600704	0.0	0.0	30.312412046158176	0.0
18	0.001017297447600704	0.0	0.0	31.870742186307854	0.0
19	0.001017297447600704	0.0	0.0	33.29716275741864	0.0
20	0.001017297447600704	0.0	0.0	34.28478902946433	0.0
21	0.001017297447600704	0.0	0.0	35.36787171200987	0.0
22	0.001017297447600704	0.0	0.0	36.59964937147973	0.0
23	0.001017297447600704	0.0	0.0	37.74360035130672	0.0
24	0.0011868470222008212	0.0	0.0	38.67697075948036	0.0
25	0.0011868470222008212	0.0	0.0	39.475718805421515	0.0
26	0.0011868470222008212	0.0	0.0	40.309563613304896	0.0
27	0.0011868470222008212	0.0	0.0	41.11967148074425	0.0
28	0.0011868470222008212	0.0	0.0	41.879084025378184	0.0
29	0.0011868470222008212	0.0	0.0	42.692921983458746	0.0
30	0.0011868470222008212	0.0	0.0	43.58254860138556	0.0
31	0.0011868470222008212	0.0	0.0	44.40944187671033	0.0
32	0.0011868470222008212	0.0	0.0	45.161902888785654	0.0
33	0.0011868470222008212	0.0	0.0	45.90876876489917	0.0
34	0.0011868470222008212	0.0	0.0	46.65122635207308	0.0
35	0.0011868470222008212	0.0	0.0	47.37418573816798	0.0
36	0.0011868470222008212	0.0	0.0	48.05085809039705	0.0
37	0.0011868470222008212	0.0	0.0	48.76974828670155	0.0
38	0.0011868470222008212	0.0	0.0	49.491690375348846	0.0
39	0.0011868470222008212	0.0	0.0	50.227705078687954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.0304895E-4	45.0	4
CGGGTCG	35	1.2105738E-7	45.0	6
CCTAGTG	20	7.0304895E-4	45.0	29
TTGTACG	20	7.0304895E-4	45.0	1
CGATTCC	20	7.0304895E-4	45.0	21
CTAAGCG	25	3.8884886E-5	45.0	1
TCGATTC	20	7.0304895E-4	45.0	20
TGGCCGT	20	7.0304895E-4	45.0	33
ACCTCGA	20	7.0304895E-4	45.0	19
GTGCTCG	20	7.0304895E-4	45.0	33
CGGATCC	40	6.8048394E-9	45.0	6
TACCTCG	20	7.0304895E-4	45.0	18
TGTTACG	20	7.0304895E-4	45.0	1
AAATTCG	20	7.0304895E-4	45.0	33
TCGTACA	20	7.0304895E-4	45.0	37
CCCCACG	20	7.0304895E-4	45.0	38
GTATACG	25	3.8884886E-5	45.0	1
ATGCGCG	20	7.0304895E-4	45.0	1
TTACGGT	20	7.0304895E-4	45.0	1
CGCACTT	35	1.2105738E-7	45.0	35
>>END_MODULE