##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351151
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79862224513101	33.0	31.0	34.0	30.0	34.0
2	31.83970143898209	33.0	31.0	34.0	30.0	34.0
3	31.869167395223137	33.0	31.0	34.0	30.0	34.0
4	35.59443942919143	37.0	35.0	37.0	33.0	37.0
5	33.62085826325427	37.0	35.0	37.0	32.0	37.0
6	34.56368912519115	37.0	35.0	37.0	30.0	37.0
7	35.66167261377584	37.0	35.0	37.0	32.0	37.0
8	35.673613345825586	37.0	35.0	37.0	33.0	37.0
9	37.501892348306	39.0	37.0	39.0	35.0	39.0
10	37.05616387252208	39.0	37.0	39.0	33.0	39.0
11	37.08533081210078	39.0	37.0	39.0	34.0	39.0
12	37.00099102665235	39.0	37.0	39.0	33.0	39.0
13	36.90655586912752	39.0	37.0	39.0	33.0	39.0
14	37.92034196115062	40.0	37.0	41.0	33.0	41.0
15	38.072273751178265	40.0	37.0	41.0	33.0	41.0
16	38.08006527106572	40.0	37.0	41.0	34.0	41.0
17	38.09609825972303	40.0	37.0	41.0	34.0	41.0
18	38.07955551885087	40.0	37.0	41.0	34.0	41.0
19	37.97804363365048	40.0	36.0	41.0	34.0	41.0
20	38.03359808173692	40.0	36.0	41.0	34.0	41.0
21	37.90492409248443	40.0	36.0	41.0	34.0	41.0
22	37.96654430714992	40.0	36.0	41.0	34.0	41.0
23	37.97448106370194	40.0	36.0	41.0	34.0	41.0
24	37.93268707763897	40.0	36.0	41.0	34.0	41.0
25	37.729760131681246	39.0	36.0	41.0	33.0	41.0
26	37.82011727148719	40.0	36.0	41.0	34.0	41.0
27	37.78404731867487	40.0	36.0	41.0	34.0	41.0
28	37.634003605286615	40.0	36.0	41.0	33.0	41.0
29	37.657284188283676	40.0	36.0	41.0	33.0	41.0
30	37.50636335935253	39.0	36.0	41.0	33.0	41.0
31	37.40200939197098	39.0	35.0	41.0	33.0	41.0
32	37.31860082984243	39.0	35.0	41.0	33.0	41.0
33	37.24570911089531	39.0	35.0	41.0	32.0	41.0
34	37.17834492853502	39.0	35.0	41.0	32.0	41.0
35	37.09075298091135	39.0	35.0	41.0	32.0	41.0
36	36.93362969206979	39.0	35.0	41.0	31.0	41.0
37	36.80092324954222	39.0	35.0	41.0	31.0	41.0
38	36.66271774820519	39.0	35.0	41.0	31.0	41.0
39	36.69744639770355	39.0	35.0	41.0	31.0	41.0
40	36.642760521826794	39.0	35.0	41.0	31.0	41.0
41	36.54667650099245	39.0	35.0	41.0	31.0	41.0
42	36.5316032134324	39.0	35.0	41.0	31.0	41.0
43	36.42751408938035	39.0	35.0	40.0	31.0	41.0
44	36.21724557241756	38.0	35.0	40.0	30.0	41.0
45	36.19526357606841	38.0	35.0	40.0	30.0	41.0
46	36.08916961648977	38.0	35.0	40.0	30.0	41.0
47	36.0039868888313	38.0	35.0	40.0	30.0	41.0
48	35.92092575558663	38.0	35.0	40.0	29.0	41.0
49	35.82537426918904	38.0	35.0	40.0	29.0	41.0
50	35.68752758784682	38.0	35.0	40.0	29.0	41.0
51	34.341012840629816	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	4.0
11	5.0
12	13.0
13	2.0
14	4.0
15	11.0
16	19.0
17	29.0
18	57.0
19	130.0
20	211.0
21	374.0
22	588.0
23	939.0
24	1432.0
25	2090.0
26	2857.0
27	3270.0
28	3883.0
29	4725.0
30	5788.0
31	7504.0
32	9798.0
33	13899.0
34	23898.0
35	32376.0
36	28859.0
37	43734.0
38	72252.0
39	92369.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.850226825496726	19.93558326759713	22.965334001611843	14.248855905294302
2	33.64848740285518	23.011752778719128	24.95080463959949	18.3889551788262
3	31.848976651070338	23.233594664403633	28.163952259854025	16.753476424672005
4	28.677976141318123	25.415277188445994	26.480630839724224	19.426115830511662
5	22.892430891553776	33.722529624007905	23.888868321605237	19.496171162833082
6	24.009614097638906	33.85979251091411	25.91136007016924	16.21923332127774
7	77.18531344065659	6.041560468288571	11.071875062295138	5.701251028759708
8	76.39249211877511	5.130271592562743	10.80503828837167	7.672198000290472
9	70.03625221058746	7.0263220096197925	14.533633678958624	8.403792100834114
10	38.623555108770866	24.62530364430117	21.488761245162337	15.26238000176562
11	29.138746579106993	23.300802218988412	26.846285501109207	20.714165700795384
12	26.33340073073977	20.688250923391934	32.86591808082563	20.112430265042676
13	22.686821338968137	25.357752078165802	33.756133401300296	18.199293181565764
14	19.52379460687852	28.814669472676997	30.619021446614138	21.042514473830344
15	16.256254431854103	24.641535977400036	38.69133221890298	20.410877371842883
16	18.6930978410997	23.882318432810955	34.40713539189693	23.017448334192416
17	19.536894384467082	23.873775099601026	30.07623501000994	26.513095505921953
18	21.07782691776472	23.413858995133147	33.584127626007046	21.924186461095086
19	21.36716113580767	25.601237074648797	30.010166566519814	23.021435223023715
20	22.45187967569507	25.73394351717637	29.835882569037253	21.978294238091305
21	20.922053475570337	27.06328616464142	31.56106632189571	20.45359403789253
22	20.132079931425512	24.49943186834154	28.06741259458182	27.301075605651132
23	18.389809512147195	26.72126805847057	29.01885513639432	25.87006729298792
24	20.97274391928259	24.086504096528273	31.608908987871313	23.33184299631782
25	18.72613206284476	26.54356672770404	29.390490131026255	25.33981107842495
26	19.67529638246794	26.218065732405716	29.697195793262726	24.409442091863614
27	22.447892786863772	25.739923850423324	28.884155249451094	22.928028113261817
28	18.553556732004182	25.026270749620533	32.31373397769051	24.106438540684778
29	21.42497102386153	23.925604654407934	30.07167856563131	24.57774575609923
30	26.071405178968593	24.49088853513161	28.44816047797101	20.989545807928785
31	25.525770964627753	25.87206073740357	26.36728928580582	22.23487901216286
32	24.914068306796793	28.259637591805237	25.127651637045034	21.698642464352943
33	25.474795743141836	26.19329006609692	25.82621151584361	22.50570267491763
34	24.151718206697403	24.125518651520288	28.920322026706458	22.80244111507585
35	24.47380186871175	26.810118723853844	26.136049733590394	22.580029673844017
36	28.617603253301287	25.497008409487655	26.86878294522869	19.016605391982367
37	23.957784542832	27.558514713043675	29.163807023189452	19.319893720934868
38	22.102742125182615	29.81936545816472	26.51024772818531	21.567644688467354
39	24.73465830938827	27.233868051066352	27.012880498702835	21.018593140842544
40	26.521069283584552	24.362453759209004	27.85667704207022	21.259799915136224
41	22.570062451765764	26.661464726001068	25.483054298578107	25.285418523655068
42	23.953512876227038	25.897690737033358	27.442610159162296	22.70618622757731
43	23.911650543498382	24.93030063989566	28.609629475638688	22.54841934096728
44	22.03069334844554	24.548128867638138	28.971866803739704	24.44931098017662
45	23.167241443139847	24.316604537649045	26.84030516786226	25.67584885134885
46	26.55638172751893	24.294107093529565	27.810827820510266	21.33868335844124
47	22.418560676176348	24.58771297817748	30.68452033455693	22.30920601108925
48	22.250257011940732	23.73081665722154	29.084638802110774	24.93428752872696
49	23.109716332859655	22.13805456911699	31.62485654319652	23.12737255482684
50	22.6791323390792	22.162830235425783	30.599656558004963	24.558380867490055
51	21.854131128773663	22.59540767362189	26.85226583435616	28.698195363248292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	318.5
2	480.0
3	1332.0
4	2184.0
5	1442.0
6	700.0
7	725.0
8	750.0
9	809.5
10	869.0
11	875.0
12	881.0
13	910.0
14	939.0
15	888.5
16	838.0
17	856.5
18	875.0
19	832.0
20	789.0
21	1024.0
22	1259.0
23	1260.0
24	1261.0
25	1397.0
26	1987.0
27	2441.0
28	2622.0
29	2803.0
30	3383.0
31	3963.0
32	4619.5
33	5276.0
34	6028.0
35	6780.0
36	7699.5
37	8619.0
38	9514.0
39	10409.0
40	12310.0
41	14211.0
42	17090.0
43	19969.0
44	22581.0
45	25193.0
46	30309.5
47	35426.0
48	40417.0
49	45408.0
50	45725.0
51	46042.0
52	37929.5
53	29817.0
54	24972.0
55	20127.0
56	17575.0
57	15023.0
58	13612.5
59	12202.0
60	11173.5
61	10145.0
62	8789.0
63	7433.0
64	6388.0
65	5343.0
66	4477.0
67	3611.0
68	2934.5
69	2258.0
70	2075.5
71	1893.0
72	1580.0
73	1267.0
74	1047.5
75	736.5
76	645.0
77	455.5
78	266.0
79	176.5
80	87.0
81	94.0
82	101.0
83	64.5
84	28.0
85	19.0
86	10.0
87	9.5
88	9.0
89	5.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.27611518983677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6253367238419	25.620128503150973
2	12.790379787586401	9.279705809924886
3	4.878604450657868	5.309304510531455
4	2.4102050911681463	3.4973151007338688
5	1.4256550213429755	2.585861288760349
6	0.9192249446545682	2.0007545986596273
7	0.662760525872395	1.6829664025876656
8	0.5035184130210558	1.4612553560764503
9	0.41525227468795683	1.3557365414479856
>10	5.2138374904739235	39.88642502909829
>50	0.11267621607649889	2.633407450401357
>100	0.03860933278145766	2.533364835874007
>500	7.879455669685237E-4	0.18893762343593803
>1k	0.003151782267874095	1.9648369493171527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	2388	0.6800493235103987	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	2030	0.5780988805385717	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1242	0.3536939948910867	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	1214	0.3457202172284857	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	661	0.18823810839211622	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	461	0.131282553659252	No Hit
ACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	449	0.12786522037528014	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	412	0.11732844274970028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8477777366432103E-4	0.0	0.0	0.3665089947059812	0.0
2	2.8477777366432103E-4	0.0	0.0	2.057804192498384	0.0
3	2.8477777366432103E-4	0.0	0.0	2.750098960276348	0.0
4	2.8477777366432103E-4	0.0	0.0	3.5608612818986702	0.0
5	2.8477777366432103E-4	0.0	0.0	5.730013583899804	0.0
6	2.8477777366432103E-4	0.0	0.0	7.050812898154925	0.0
7	2.8477777366432103E-4	0.0	0.0	8.39895087868182	0.0
8	5.695555473286421E-4	0.0	0.0	10.780547399836538	0.0
9	5.695555473286421E-4	0.0	0.0	11.692405831109694	0.0
10	5.695555473286421E-4	0.0	0.0	13.671326580303061	0.0
11	5.695555473286421E-4	0.0	0.0	16.034982101716924	0.0
12	5.695555473286421E-4	0.0	0.0	18.026717850725188	0.0
13	5.695555473286421E-4	0.0	0.0	18.862255838656303	0.0
14	5.695555473286421E-4	0.0	0.0	19.18149172293401	0.0
15	5.695555473286421E-4	0.0	0.0	19.770981714419154	0.0
16	5.695555473286421E-4	0.0	0.0	20.965339697167316	0.0
17	5.695555473286421E-4	0.0	0.0	22.402613120851143	0.0
18	5.695555473286421E-4	0.0	0.0	24.043787430478627	0.0
19	5.695555473286421E-4	0.0	0.0	24.938559195331923	0.0
20	5.695555473286421E-4	0.0	0.0	25.80627707168711	0.0
21	5.695555473286421E-4	0.0	0.0	26.841729056730582	0.0
22	5.695555473286421E-4	0.0	0.0	27.901102374761855	0.0
23	5.695555473286421E-4	0.0	0.0	28.959051803924808	0.0
24	5.695555473286421E-4	0.0	0.0	29.74276023704902	0.0
25	5.695555473286421E-4	0.0	0.0	30.455843782304477	0.0
26	5.695555473286421E-4	0.0	0.0	31.137032216909535	0.0
27	5.695555473286421E-4	0.0	0.0	31.818505429288255	0.0
28	8.543333209929631E-4	0.0	0.0	32.49428308619369	0.0
29	8.543333209929631E-4	0.0	0.0	33.17718018744073	0.0
30	8.543333209929631E-4	0.0	0.0	33.964305953848914	0.0
31	8.543333209929631E-4	0.0	0.0	34.67311783249941	0.0
32	8.543333209929631E-4	0.0	0.0	35.3668364891457	0.0
33	8.543333209929631E-4	0.0	0.0	36.04460759046678	0.0
34	8.543333209929631E-4	0.0	0.0	36.67539035913325	0.0
35	0.0011391110946572841	0.0	0.0	37.42492545941774	0.0
36	0.0011391110946572841	0.0	0.0	38.016693673092206	0.0
37	0.0011391110946572841	0.0	0.0	38.660006663799905	0.0
38	0.0011391110946572841	0.0	0.0	39.2634507661946	0.0
39	0.0011391110946572841	0.0	0.0	39.88369675723549	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.026587E-4	45.000004	1
GGTAAGT	40	6.7921064E-9	45.000004	8
ACGTTAG	20	7.026587E-4	45.000004	1
CCGATAT	20	7.026587E-4	45.000004	11
TCTACCC	20	7.026587E-4	45.000004	30
ACTCGTC	20	7.026587E-4	45.000004	30
CCGACTC	20	7.026587E-4	45.000004	27
ATCGGTT	20	7.026587E-4	45.000004	34
GATTCGA	20	7.026587E-4	45.000004	9
GCGTATG	20	7.026587E-4	45.000004	1
CCCGCAA	20	7.026587E-4	45.000004	12
CGTATGG	25	3.885255E-5	44.999996	2
TTTAACG	25	3.885255E-5	44.999996	1
CGCATGG	50	2.1827873E-11	44.999996	2
CTACGAA	30	2.1611813E-6	44.999996	11
CTTAGTA	25	3.885255E-5	44.999996	41
GCGAGCT	25	3.885255E-5	44.999996	20
ACGGGCG	80	0.0	42.187504	5
TAGGGCG	60	3.6379788E-12	41.249996	5
CACGGGA	50	1.0768417E-9	40.499996	4
>>END_MODULE