SRR2935872.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583327
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	7222	1.238070584766349	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	7113	1.2193846676049624	RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	6074	1.041268448057436	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	3768	0.6459498703128776	RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2128	0.36480396072871646	RNA PCR Primer, Index 17 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	1746	0.2993175354475277	TruSeq Adapter, Index 9 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.2900602920831712	No Hit
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	1601	0.2744601227099037	RNA PCR Primer, Index 17 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	1527	0.2617742706920818	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAGATGT	1465	0.2511455838663391	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC	1272	0.21805951036039822	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTA	1042	0.17863051084554632	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	863	0.14794446339703116	RNA PCR Primer, Index 17 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	848	0.14537300690693214	RNA PCR Primer, Index 47 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	800	0.1371443461386152	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	40	6.8048394E-9	45.000004	34
TTAATCG	20	7.0304255E-4	45.000004	1
ACGTTAG	20	7.0304255E-4	45.000004	1
TAGCGAG	40	6.8048394E-9	45.000004	1
TCGTGCG	40	6.8048394E-9	45.000004	1
TTGCACG	20	7.0304255E-4	45.000004	1
CGATATT	20	7.0304255E-4	45.000004	10
TAATGCG	20	7.0304255E-4	45.000004	1
GCCCGAC	20	7.0304255E-4	45.000004	9
AATCGCG	30	2.1636552E-6	45.000004	1
GAGTACG	25	3.8884355E-5	45.0	1
GCGATAT	50	2.1827873E-11	45.0	9
TAGTGCG	25	3.8884355E-5	45.0	1
CGTTATT	130	0.0	44.999996	1
CGTTTTT	1405	0.0	42.43772	1
CGTGCGG	70	0.0	41.785713	2
AGCGATG	70	0.0	41.785713	1
GCGTCAT	70	0.0	41.785713	37
ATGGGCG	180	0.0	40.0	5
GGTCCGA	45	1.9255822E-8	40.0	8