##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583327
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7128557395766	31.0	31.0	34.0	30.0	34.0
2	31.768025481419514	33.0	31.0	34.0	30.0	34.0
3	31.80420415993088	33.0	31.0	34.0	30.0	34.0
4	35.54257046219359	37.0	35.0	37.0	33.0	37.0
5	33.51374957785256	37.0	35.0	37.0	32.0	37.0
6	34.473336567654165	37.0	35.0	37.0	30.0	37.0
7	35.57773770115218	37.0	35.0	37.0	32.0	37.0
8	35.58734809120785	37.0	35.0	37.0	33.0	37.0
9	37.45885412470192	39.0	37.0	39.0	35.0	39.0
10	36.78419822843791	39.0	35.0	39.0	32.0	39.0
11	36.914673930745536	39.0	37.0	39.0	33.0	39.0
12	36.957204106787444	39.0	37.0	39.0	33.0	39.0
13	36.980744933802136	39.0	37.0	39.0	33.0	39.0
14	38.05849206362812	40.0	37.0	41.0	33.0	41.0
15	38.10762573993661	40.0	37.0	41.0	33.0	41.0
16	38.11844814315127	40.0	37.0	41.0	33.0	41.0
17	38.05811320237191	40.0	37.0	41.0	33.0	41.0
18	38.03312035959247	40.0	37.0	41.0	33.0	41.0
19	37.90879558120917	40.0	37.0	41.0	33.0	41.0
20	37.9086824371236	40.0	36.0	41.0	33.0	41.0
21	37.928126076797405	40.0	36.0	41.0	33.0	41.0
22	38.02339682545125	40.0	37.0	41.0	34.0	41.0
23	37.99495823097508	40.0	37.0	41.0	34.0	41.0
24	37.95480408073002	40.0	36.0	41.0	33.0	41.0
25	37.80864249383279	40.0	36.0	41.0	33.0	41.0
26	37.85501785447956	40.0	36.0	41.0	33.0	41.0
27	37.841087417520534	40.0	36.0	41.0	33.0	41.0
28	37.769477497184255	40.0	36.0	41.0	33.0	41.0
29	37.77351639817804	40.0	36.0	41.0	33.0	41.0
30	37.630222842419435	40.0	36.0	41.0	33.0	41.0
31	37.56834845635467	40.0	36.0	41.0	33.0	41.0
32	37.448810015651596	40.0	36.0	41.0	33.0	41.0
33	37.365592540719014	40.0	36.0	41.0	33.0	41.0
34	37.2277110437199	40.0	36.0	41.0	32.0	41.0
35	37.14651816219719	40.0	36.0	41.0	32.0	41.0
36	37.06508184946008	40.0	36.0	41.0	31.0	41.0
37	36.94554683736566	39.0	36.0	41.0	31.0	41.0
38	36.8985628986829	39.0	35.0	41.0	31.0	41.0
39	36.86321222916135	39.0	35.0	41.0	31.0	41.0
40	36.82574782240493	39.0	35.0	41.0	31.0	41.0
41	36.71804493877362	39.0	35.0	41.0	31.0	41.0
42	36.782509638676075	39.0	35.0	41.0	31.0	41.0
43	36.72299756397355	39.0	35.0	41.0	31.0	41.0
44	36.58750409290158	39.0	35.0	41.0	30.0	41.0
45	36.58671379860696	39.0	35.0	41.0	30.0	41.0
46	36.49750311574812	39.0	35.0	41.0	30.0	41.0
47	36.38552647143026	39.0	35.0	41.0	30.0	41.0
48	36.33343390585384	39.0	35.0	40.0	30.0	41.0
49	36.27298239238026	39.0	35.0	40.0	30.0	41.0
50	36.14537814981991	39.0	35.0	40.0	29.0	41.0
51	34.77104094273024	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	7.0
11	9.0
12	6.0
13	9.0
14	14.0
15	15.0
16	21.0
17	57.0
18	123.0
19	279.0
20	582.0
21	980.0
22	1468.0
23	1982.0
24	2673.0
25	3771.0
26	5082.0
27	5720.0
28	6194.0
29	7305.0
30	9158.0
31	11652.0
32	15626.0
33	21636.0
34	34737.0
35	45713.0
36	46126.0
37	69870.0
38	120790.0
39	171641.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.102832545039064	19.366153118233857	23.071279059601217	12.459735277125866
2	29.966553922585444	28.782655354543852	24.923413454203217	16.32737726866749
3	29.396376303514156	27.93630330843592	27.035950676035913	15.631369712014015
4	26.03479694922402	24.148033607222022	32.02526198855873	17.791907454995226
5	25.539534429230947	31.62222904134388	25.00741436621312	17.830822163212055
6	21.671035285525956	37.50435004722908	26.877377525813138	13.94723714143182
7	75.32670354706708	5.123541341305991	14.72107411451896	4.828680997107969
8	75.47996235387699	7.649225905881265	10.446456275810995	6.424355464430756
9	70.93208440548783	5.61846100043372	12.582993758217947	10.866460835860503
10	37.82509638676077	19.726842748578413	24.228091619280438	18.21996924538038
11	29.235403127233955	24.58329547577945	25.0415290223151	21.139772374671498
12	22.7956189238626	21.40600383661308	32.50063857836171	23.297738661162608
13	22.435443584816063	25.06501499159134	35.58792924037461	16.91161218321799
14	20.90165550368833	29.390033377505244	30.326729261631986	19.38158185717445
15	17.960595000745723	25.85239496885966	37.745895526865716	18.441114503528897
16	19.54889795946356	25.34856092723292	31.255539345855755	23.84700176744776
17	20.515251308442778	26.050225688164613	30.101298242666637	23.333224760725972
18	21.033142645548722	25.707364822818075	32.083719766100316	21.175772765532884
19	20.261362837653664	29.06654415105078	28.702254481620088	21.969838529675464
20	20.92497004253189	27.56207067391017	31.682058262346846	19.830901021211087
21	19.97250255859921	29.92592491004188	30.837077659700306	19.26449487165861
22	18.65574540523583	25.158958868696292	29.76186598597356	26.423429740094324
23	17.943623387911067	29.168888119356723	29.75860880775277	23.128879684979438
24	20.707767684334858	25.293703188777478	30.020554508877524	23.97797461801014
25	18.059853221263545	31.016051031411195	27.54338475674879	23.38071099057647
26	19.3985534700091	26.916635094895312	30.865020820226047	22.819790614869532
27	21.884466174204174	27.177552213424033	29.372890334237916	21.565091278133877
28	19.540669298695242	26.47571602205967	31.49742768635774	22.48618699288735
29	22.530416044517054	24.876098654785395	28.863056227467617	23.73042907322994
30	22.77281867631706	26.39120081875175	30.383987026144855	20.45199347878634
31	23.99323192651806	26.747433257846797	26.29314261126264	22.966192204372504
32	23.811515667884393	29.295746639534944	26.985207267964622	19.907530424616038
33	24.62032444923688	25.654735679987382	26.733718823232937	22.9912210475428
34	24.501866020259648	27.357554167730964	27.124580209727988	21.015999602281397
35	21.99675310760517	26.031882631868577	29.806780759333957	22.164583501192297
36	28.86768484914979	25.053186291736885	27.364411385037897	18.71471747407543
37	21.420403992957638	28.563567261587412	31.272510958690408	18.743517786764542
38	24.287406548985388	27.444126536230968	27.122694474968583	21.14577243981506
39	24.7516401606646	28.278649882484437	26.87652037364977	20.09318958320119
40	23.45819754614479	24.70758253946757	30.849763511718127	20.984456402669515
41	22.525615992402205	26.927778073019077	27.531213196028986	23.015392738549735
42	23.48082636325766	23.55334143627845	31.99371878894685	20.97211341151704
43	26.274628124533923	23.626027939731916	28.630425130329982	21.468918805404172
44	22.75036128963686	24.992328488137872	28.59013897865177	23.667171243573502
45	22.309442216801212	24.199462737024003	27.78150162773196	25.70959341844283
46	26.076454544363624	25.831651886506197	27.497955692090372	20.59393787703981
47	21.112514935876447	24.113061798956675	33.39910547600231	21.37531778916457
48	22.321956638386357	24.28843513158143	29.582206892531975	23.807401337500238
49	23.437797324656668	21.58360576486259	32.87538550418547	22.103211406295266
50	23.282481352654685	21.774236405995264	30.546331645886443	24.396950595463608
51	21.76189341484279	21.954752651600216	27.697500715722057	28.585853217834938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	236.5
2	383.0
3	1904.0
4	3425.0
5	2462.5
6	1500.0
7	1443.0
8	1386.0
9	1428.5
10	1471.0
11	1432.5
12	1394.0
13	1366.0
14	1338.0
15	1342.0
16	1346.0
17	1334.5
18	1323.0
19	1250.0
20	1177.0
21	1386.0
22	1595.0
23	1795.0
24	1995.0
25	2140.0
26	3056.0
27	3827.0
28	4503.5
29	5180.0
30	5939.0
31	6698.0
32	8298.0
33	9898.0
34	11505.0
35	13112.0
36	14620.0
37	16128.0
38	18137.0
39	20146.0
40	22872.5
41	25599.0
42	29931.5
43	34264.0
44	40183.5
45	46103.0
46	66730.0
47	87357.0
48	82015.0
49	76673.0
50	75691.5
51	74710.0
52	61771.5
53	48833.0
54	40739.0
55	32645.0
56	27209.0
57	21773.0
58	18149.5
59	14526.0
60	12112.5
61	9699.0
62	8142.0
63	6585.0
64	5347.5
65	4110.0
66	3133.0
67	2156.0
68	1548.0
69	940.0
70	814.0
71	688.0
72	546.5
73	405.0
74	360.0
75	238.5
76	162.0
77	101.5
78	41.0
79	30.0
80	19.0
81	16.0
82	13.0
83	9.0
84	5.0
85	3.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	583327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.32736671286883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0326154282044	30.59573046514194
2	11.486673132247342	9.494279056944869
3	4.235194572427197	5.2508831758515155
4	2.2272635448466227	3.681877491363222
5	1.35956389016861	2.8093597729286333
6	0.925838341687334	2.2957476398248087
7	0.7303269163892652	2.1127741805728495
8	0.6182662760055023	2.044105369174334
9	0.46416661171688717	1.7264505400464238
>10	3.812149189875373	28.751989214972905
>50	0.0658488464908746	1.8302003330228727
>100	0.03500824747532684	2.474886934864264
>500	0.0020838242544837407	0.6191953880462822
>1k	0.0037508836580707333	2.7973163552878075
>5k	0.0012502945526902444	3.5152040819573216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	7222	1.238070584766349	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	7113	1.2193846676049624	RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	6074	1.041268448057436	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	3768	0.6459498703128776	RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2128	0.36480396072871646	RNA PCR Primer, Index 17 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	1746	0.2993175354475277	TruSeq Adapter, Index 9 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.2900602920831712	No Hit
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	1601	0.2744601227099037	RNA PCR Primer, Index 17 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	1527	0.2617742706920818	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAGATGT	1465	0.2511455838663391	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC	1272	0.21805951036039822	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTA	1042	0.17863051084554632	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	863	0.14794446339703116	RNA PCR Primer, Index 17 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	848	0.14537300690693214	RNA PCR Primer, Index 47 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	800	0.1371443461386152	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4286086534653803E-4	0.0	0.0	0.29023172251584445	0.0
2	3.4286086534653803E-4	0.0	0.0	1.6203604496277388	0.0
3	3.4286086534653803E-4	0.0	0.0	2.1502519170208134	0.0
4	3.4286086534653803E-4	0.0	0.0	3.100147944463397	0.0
5	3.4286086534653803E-4	0.0	0.0	5.9429788094842175	0.0
6	3.4286086534653803E-4	0.0	0.0	7.365679970239677	0.0
7	3.4286086534653803E-4	0.0	0.0	8.814609987194146	0.0
8	3.4286086534653803E-4	0.0	0.0	11.051262842282288	0.0
9	3.4286086534653803E-4	0.0	0.0	11.822356928446652	0.0
10	3.4286086534653803E-4	0.0	0.0	14.581872603188263	0.0
11	3.4286086534653803E-4	0.0	0.0	16.69715271194373	0.0
12	3.4286086534653803E-4	0.0	0.0	19.680385101323957	0.0
13	3.4286086534653803E-4	0.0	0.0	20.54062301247842	0.0
14	3.4286086534653803E-4	0.0	0.0	20.912284190514068	0.0
15	3.4286086534653803E-4	0.0	0.0	21.584805777891305	0.0
16	3.4286086534653803E-4	0.0	0.0	22.721732407380422	0.0
17	5.14291298019807E-4	0.0	0.0	24.098318781746773	0.0
18	5.14291298019807E-4	0.0	0.0	25.54073444225966	0.0
19	5.14291298019807E-4	0.0	0.0	26.66411806756759	0.0
20	5.14291298019807E-4	0.0	0.0	27.52041307877057	0.0
21	5.14291298019807E-4	0.0	0.0	28.542481318368598	0.0
22	5.14291298019807E-4	0.0	0.0	29.69089378684683	0.0
23	5.14291298019807E-4	0.0	0.0	30.91079274574981	0.0
24	5.14291298019807E-4	0.0	0.0	31.77548784815378	0.0
25	5.14291298019807E-4	0.0	0.0	32.562353534124085	0.0
26	5.14291298019807E-4	0.0	0.0	33.292132886014194	0.0
27	5.14291298019807E-4	0.0	0.0	34.044198194151825	0.0
28	5.14291298019807E-4	0.0	0.0	34.789063424117174	0.0
29	5.14291298019807E-4	0.0	0.0	35.55484316686867	0.0
30	5.14291298019807E-4	0.0	0.0	36.41525250845581	0.0
31	5.14291298019807E-4	0.0	0.0	37.22046125072215	0.0
32	5.14291298019807E-4	0.0	0.0	37.95966927640929	0.0
33	5.14291298019807E-4	0.0	0.0	38.6688769763786	0.0
34	5.14291298019807E-4	0.0	0.0	39.35322726361029	0.0
35	5.14291298019807E-4	0.0	0.0	40.05112055502317	0.0
36	5.14291298019807E-4	0.0	0.0	40.7243278641311	0.0
37	5.14291298019807E-4	0.0	0.0	41.40079235145981	0.0
38	5.14291298019807E-4	0.0	0.0	42.094742742921206	0.0
39	5.14291298019807E-4	0.0	0.0	42.77189295198062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	40	6.8048394E-9	45.000004	34
TTAATCG	20	7.0304255E-4	45.000004	1
ACGTTAG	20	7.0304255E-4	45.000004	1
TAGCGAG	40	6.8048394E-9	45.000004	1
TCGTGCG	40	6.8048394E-9	45.000004	1
TTGCACG	20	7.0304255E-4	45.000004	1
CGATATT	20	7.0304255E-4	45.000004	10
TAATGCG	20	7.0304255E-4	45.000004	1
GCCCGAC	20	7.0304255E-4	45.000004	9
AATCGCG	30	2.1636552E-6	45.000004	1
GAGTACG	25	3.8884355E-5	45.0	1
GCGATAT	50	2.1827873E-11	45.0	9
TAGTGCG	25	3.8884355E-5	45.0	1
CGTTATT	130	0.0	44.999996	1
CGTTTTT	1405	0.0	42.43772	1
CGTGCGG	70	0.0	41.785713	2
AGCGATG	70	0.0	41.785713	1
GCGTCAT	70	0.0	41.785713	37
ATGGGCG	180	0.0	40.0	5
GGTCCGA	45	1.9255822E-8	40.0	8
>>END_MODULE