##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935871.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587677
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.863763598030893	33.0	31.0	34.0	30.0	34.0
2	31.91169639104474	33.0	31.0	34.0	30.0	34.0
3	31.96324001109453	33.0	31.0	34.0	30.0	34.0
4	35.67173464335001	37.0	35.0	37.0	33.0	37.0
5	33.68259435029787	37.0	35.0	37.0	32.0	37.0
6	34.602836251886664	37.0	35.0	37.0	30.0	37.0
7	35.66642730615627	37.0	35.0	37.0	32.0	37.0
8	35.64730795998482	37.0	35.0	37.0	33.0	37.0
9	37.51266256804333	39.0	37.0	39.0	35.0	39.0
10	37.07232204084897	39.0	37.0	39.0	33.0	39.0
11	37.085450000595564	39.0	37.0	39.0	34.0	39.0
12	37.02520432142146	39.0	37.0	39.0	33.0	39.0
13	36.96711458845591	39.0	37.0	39.0	33.0	39.0
14	37.98716471803389	40.0	37.0	41.0	33.0	41.0
15	38.065549613137826	40.0	37.0	41.0	33.0	41.0
16	38.10724598716642	40.0	37.0	41.0	33.0	41.0
17	38.057182772169064	40.0	37.0	41.0	33.0	41.0
18	38.05630984367263	40.0	37.0	41.0	33.0	41.0
19	37.95251132850188	40.0	37.0	41.0	33.0	41.0
20	37.98934278523747	40.0	37.0	41.0	33.0	41.0
21	37.89651458198977	40.0	36.0	41.0	33.0	41.0
22	37.98654022532786	40.0	37.0	41.0	34.0	41.0
23	37.972166683399216	40.0	36.0	41.0	34.0	41.0
24	37.903741340906656	40.0	36.0	41.0	33.0	41.0
25	37.72518747543293	40.0	36.0	41.0	33.0	41.0
26	37.78367708792415	40.0	36.0	41.0	33.0	41.0
27	37.78213372311661	40.0	36.0	41.0	33.0	41.0
28	37.67713727098389	40.0	36.0	41.0	33.0	41.0
29	37.67037505296277	40.0	36.0	41.0	33.0	41.0
30	37.54530464864202	40.0	36.0	41.0	33.0	41.0
31	37.526362270430866	40.0	36.0	41.0	33.0	41.0
32	37.42336861915644	40.0	36.0	41.0	33.0	41.0
33	37.38192748737827	40.0	36.0	41.0	33.0	41.0
34	37.27916185251422	40.0	35.0	41.0	32.0	41.0
35	37.216142540885556	40.0	35.0	41.0	32.0	41.0
36	37.12842769072126	39.0	35.0	41.0	32.0	41.0
37	37.07897875873992	39.0	35.0	41.0	32.0	41.0
38	36.97877745768509	39.0	35.0	41.0	32.0	41.0
39	36.872004519489444	39.0	35.0	41.0	31.0	41.0
40	36.763904321591625	39.0	35.0	41.0	31.0	41.0
41	36.61079640686976	39.0	35.0	41.0	31.0	41.0
42	36.64175729184569	39.0	35.0	41.0	31.0	41.0
43	36.60491222219008	39.0	35.0	41.0	31.0	41.0
44	36.473411414773764	39.0	35.0	40.0	31.0	41.0
45	36.379535016684336	38.0	35.0	40.0	31.0	41.0
46	36.24879653278927	38.0	35.0	40.0	30.0	41.0
47	36.14349549157105	38.0	35.0	40.0	30.0	41.0
48	36.076550554130925	38.0	35.0	40.0	30.0	41.0
49	36.01004123013152	38.0	35.0	40.0	30.0	41.0
50	35.88520735029616	38.0	35.0	40.0	30.0	41.0
51	34.671942240380346	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	12.0
11	9.0
12	7.0
13	7.0
14	14.0
15	18.0
16	36.0
17	69.0
18	135.0
19	269.0
20	452.0
21	821.0
22	1199.0
23	1703.0
24	2459.0
25	3341.0
26	4353.0
27	5108.0
28	6147.0
29	7316.0
30	9257.0
31	12042.0
32	16161.0
33	22934.0
34	38565.0
35	47113.0
36	49655.0
37	74973.0
38	121920.0
39	161491.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.54907883071823	18.749925554343626	20.230500768279175	13.470494846658966
2	30.72963549705025	26.532942415646687	23.648194501401278	19.08922758590178
3	31.88537240695144	26.781037883054807	24.43808418570065	16.895505524293107
4	27.29016109189232	25.015271994650124	27.58198806487237	20.11257884858519
5	25.24567066602913	32.637316076688386	22.628927114724583	19.4880861425579
6	23.696520367480776	36.16408333148991	24.445911614713527	15.69348468631578
7	78.2574441402335	5.420154268416153	11.487602883897106	4.834798707453244
8	77.83459281203791	7.145251558253938	9.159793560067861	5.860362069640296
9	72.59974441742658	6.223656872737916	11.13638954731936	10.040209162516144
10	38.95217951357633	25.877139993567894	18.485834905909197	16.68484558694657
11	31.060769776594967	24.565705310910584	24.225552471851035	20.147972440643414
12	25.903004541610443	22.229217750567063	28.97135671465788	22.89642099316461
13	24.621177960001837	24.101845061147532	32.205786511978516	19.07119046687211
14	21.192253567861258	27.430374168122967	29.40101450286467	21.976357761151107
15	19.18877206356553	24.31726952050191	34.941302790478446	21.55265562545412
16	22.41962846938029	24.945675941035635	29.017300319733458	23.617395269850615
17	22.23585404907798	25.387755518762855	28.487757730862363	23.8886327012968
18	24.83915484186041	22.86255885460891	29.236978816594828	23.06130748693585
19	22.84043785957252	26.86203475718805	26.454668125517927	23.842859257721503
20	25.613389668134023	25.65218649019785	28.23370661094445	20.50071723072368
21	23.162043095101563	28.417651192747034	27.582328387872927	20.837977324278473
22	23.501174965159432	22.35377596877196	28.007221654071877	26.137827411996728
23	21.442220811772454	28.678508772676146	27.38817411605355	22.491096299497855
24	22.519172947044037	24.16684675425446	28.637159528108125	24.67682077059337
25	21.788499464842083	28.272503433008268	26.313604241785875	23.62539286036377
26	22.08866435133585	24.55022061438511	27.711991451086227	25.649123583192807
27	23.228916564711568	24.745225693705898	26.35138009484802	25.674477646734516
28	19.306013337258392	26.0651684513772	28.543060218453338	26.085757992911073
29	21.052891299132007	24.479603591768946	27.081883415549697	27.38562169354935
30	21.997457787185816	24.144895920718355	27.72543420960834	26.132212082487488
31	23.6048033188299	24.71748936916027	24.164464493250545	27.513242818759288
32	22.57617704963781	25.958987675202533	25.899771473105126	25.565063802054528
33	20.877284630843132	24.307570314985956	24.184033065782735	30.631111988388177
34	19.8151365460959	26.17492261905775	26.99782363441142	27.012117200434933
35	19.14708249599695	27.10434473358665	26.443437466499454	27.30513530391695
36	20.55908262531969	28.57658203400848	23.34564735390359	27.518687986768242
37	19.51309988309905	27.727305986111418	26.638272384319954	26.121321746469572
38	19.948202839314792	28.537104565943537	24.443018869208764	27.071673725532904
39	21.357820707633614	25.9508199231891	26.937756624812607	25.753602744364677
40	22.655982793269093	25.5398799000131	26.69680794041625	25.107329366301556
41	19.393306186901988	28.724282216251446	24.55940933540023	27.323002261446337
42	21.26746495098498	26.36890672937685	26.97944619238119	25.38418212725698
43	22.266142796127806	24.81482174732038	26.562380355195113	26.356655101356697
44	21.525089462408772	24.044840958553763	27.105025379587765	27.3250441994497
45	21.34624972561458	22.70362801334747	26.996802665409742	28.953319595628212
46	23.632709804875805	24.17229192226342	25.8737367635623	26.321261509298477
47	19.416788473940617	25.10664872030044	30.346431798419882	25.130131007339067
48	21.113298631731375	26.076228948895398	26.497718985088746	26.31275343428448
49	21.992012619176858	23.145026945073568	29.62137364572716	25.241586790022406
50	20.96389683448561	23.721023623521084	28.762908876814986	26.55217066517832
51	20.64007949945293	22.756037755433685	26.1601185685334	30.44376417657999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	423.5
2	648.0
3	1583.0
4	2518.0
5	1775.5
6	1033.0
7	985.0
8	937.0
9	960.5
10	984.0
11	998.5
12	1013.0
13	1048.0
14	1083.0
15	1102.0
16	1121.0
17	1120.5
18	1120.0
19	1093.5
20	1067.0
21	1138.0
22	1209.0
23	1516.0
24	1823.0
25	2064.5
26	2730.5
27	3155.0
28	3912.0
29	4669.0
30	5529.5
31	6390.0
32	7086.5
33	7783.0
34	9706.0
35	11629.0
36	12663.5
37	13698.0
38	15287.5
39	16877.0
40	19040.0
41	21203.0
42	23956.0
43	26709.0
44	30608.0
45	34507.0
46	49096.5
47	63686.0
48	62595.5
49	61505.0
50	62871.0
51	64237.0
52	57295.5
53	50354.0
54	45664.5
55	40975.0
56	36821.0
57	32667.0
58	29359.5
59	26052.0
60	24407.0
61	22762.0
62	20465.0
63	18168.0
64	15616.5
65	13065.0
66	11061.0
67	9057.0
68	8132.5
69	7208.0
70	6275.5
71	5343.0
72	4462.5
73	3582.0
74	2937.0
75	1931.5
76	1571.0
77	1224.5
78	878.0
79	623.5
80	369.0
81	257.0
82	145.0
83	95.5
84	46.0
85	32.0
86	18.0
87	12.0
88	6.0
89	8.0
90	10.0
91	5.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.68349048630493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99099792706095	24.968990917028165
2	10.900944574855156	7.5616561490745235
3	4.012548795095187	4.175075938815547
4	1.9653885769808324	2.7266614404642833
5	1.299703624099263	2.2539129140731413
6	0.9781372706405445	2.0355128832336953
7	0.7530376789355181	1.8282582621232433
8	0.660143196391384	1.8316856217311595
9	0.6098485287720196	1.903650808117626
>10	6.709755949958021	42.478366776977786
>50	0.07425339695027998	1.7795615323976004
>100	0.03933954805293081	2.410100058866688
>500	0.0029504661039698107	0.7869366408329841
>1k	0.0024587217533081755	2.380253090477921
>5k	4.91744350661635E-4	0.8793769657856234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	5156	0.8773526954432451	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	4398	0.7483702782310692	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	4266	0.7259089601941202	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2493	0.42421262019782974	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1481	0.2520091819145551	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1318	0.22427285736892885	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	947	0.16114294076508012	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	844	0.14361630623624883	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	755	0.12847193271133633	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT	740	0.1259195102071376	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC	697	0.11860256569510123	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA	631	0.1073719066766268	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7016150027991566E-4	0.0	0.0	0.15450664225416344	0.0
2	1.7016150027991566E-4	0.0	0.0	1.034922244702447	0.0
3	1.7016150027991566E-4	0.0	0.0	1.3752452452622785	0.0
4	1.7016150027991566E-4	0.0	0.0	1.9272491521703248	0.0
5	1.7016150027991566E-4	0.0	0.0	3.8759386533759193	0.0
6	1.7016150027991566E-4	0.0	0.0	4.935023831118114	0.0
7	1.7016150027991566E-4	0.0	0.0	6.038861483433927	0.0
8	1.7016150027991566E-4	0.0	0.0	7.778592662295785	0.0
9	1.7016150027991566E-4	0.0	0.0	8.43473540737514	0.0
10	1.7016150027991566E-4	0.0	0.0	10.497092790767718	0.0
11	1.7016150027991566E-4	0.0	0.0	11.98294301121194	0.0
12	1.7016150027991566E-4	0.0	0.0	14.150800524778067	0.0
13	1.7016150027991566E-4	0.0	0.0	14.722543165718584	0.0
14	1.7016150027991566E-4	0.0	0.0	14.984762037649933	0.0
15	1.7016150027991566E-4	0.0	0.0	15.489801370480723	0.0
16	1.7016150027991566E-4	0.0	0.0	16.28326444628597	0.0
17	1.7016150027991566E-4	0.0	0.0	17.215409144819347	0.0
18	1.7016150027991566E-4	0.0	0.0	18.196390193933063	0.0
19	1.7016150027991566E-4	0.0	0.0	18.899157190089113	0.0
20	1.7016150027991566E-4	0.0	0.0	19.488766788559023	0.0
21	1.7016150027991566E-4	0.0	0.0	20.1803031256966	0.0
22	1.7016150027991566E-4	0.0	0.0	20.935650025439145	0.0
23	1.7016150027991566E-4	0.0	0.0	21.649647680613672	0.0
24	1.7016150027991566E-4	0.0	0.0	22.21696612254691	0.0
25	1.7016150027991566E-4	0.0	0.0	22.742594996911567	0.0
26	1.7016150027991566E-4	0.0	0.0	23.238615770227522	0.0
27	1.7016150027991566E-4	0.0	0.0	23.792321292138368	0.0
28	1.7016150027991566E-4	0.0	0.0	24.27779205243697	0.0
29	1.7016150027991566E-4	0.0	0.0	24.774323310253763	0.0
30	3.4032300055983133E-4	0.0	0.0	25.388776487764538	0.0
31	3.4032300055983133E-4	0.0	0.0	25.90861987111968	0.0
32	3.4032300055983133E-4	0.0	0.0	26.423528570966706	0.0
33	3.4032300055983133E-4	0.0	0.0	26.930439680300573	0.0
34	3.4032300055983133E-4	0.0	0.0	27.431224975624364	0.0
35	3.4032300055983133E-4	0.0	0.0	28.009093430574957	0.0
36	5.10484500839747E-4	0.0	0.0	28.501881135385595	0.0
37	6.806460011196627E-4	0.0	0.0	28.982757535176635	0.0
38	6.806460011196627E-4	0.0	0.0	29.490179129011345	0.0
39	6.806460011196627E-4	0.0	0.0	30.10446214502184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.0304703E-4	45.0	1
CGCTAGT	20	7.0304703E-4	45.0	16
CCATACG	20	7.0304703E-4	45.0	2
TATAGCG	20	7.0304703E-4	45.0	1
TAGGCCG	20	7.0304703E-4	45.0	1
TCGAATT	20	7.0304703E-4	45.0	41
TAACGGG	115	0.0	41.086956	3
ACAACGG	50	1.0804797E-9	40.5	2
TACGGCT	650	0.0	40.5	7
GCGATAT	50	1.0804797E-9	40.5	9
TAGCACG	45	1.9255822E-8	40.000004	1
GGAACAC	515	0.0	39.757282	8
GTTACGG	40	3.45437E-7	39.375	2
TACGAAT	40	3.45437E-7	39.375	12
CGAATAT	40	3.45437E-7	39.375	14
GCTACGA	40	3.45437E-7	39.375	10
AGGGAAC	695	0.0	38.848923	6
CGTTTTT	775	0.0	38.612904	1
CGTAAGG	105	0.0	38.57143	2
GTTAGCG	35	6.243079E-6	38.571426	1
>>END_MODULE