FastQCFastQC Report
Sat 14 Jan 2017
SRR2935870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050907
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150201.4292415979720374No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC88800.8449843801592339No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT37390.3557879051143441No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC33820.32181724929037486No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG33450.3182964810397114No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG31840.302976381354392No Hit
GAACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT28320.2694815050237557No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC26630.2534001581491036No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC26500.25216313146643804No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCT21880.20820110628247793No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC18590.17689481562117296No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT18230.17346920326917606No Hit
CGTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT15500.1474916429331996No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT14190.13502622020787758Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74950.043.619081
TTAACGG650.041.5384642
CGGTCTA650.041.53846431
GTACGAG603.6379788E-1241.2500041
CCATACG1000.040.5000042
TTGCACG501.0804797E-940.5000041
CGTTATT5250.039.428571
GTTAACG403.4578443E-739.3750041
CTCACGA750.039.00000424
ACGTAAG356.2477775E-638.5714261
CGTAAGG1350.038.3333322
TCCGAAC1250.037.80000317
TCCGATC301.1398552E-437.50000414
GTTTTTT90550.036.924352
TTACGGG1800.036.2500043
CTAATCC4600.036.19565611
AGGGCGA7900.036.1708836
ACACCCG250.002107026336.00000412
AAGCGAC250.002107026336.00000415
CGTTTCT4200.035.8928571
Produced by FastQC (version 0.11.5)