##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935870.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050907
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953433557869534	33.0	31.0	34.0	30.0	34.0
2	32.01742019036889	33.0	31.0	34.0	30.0	34.0
3	31.960051650621796	33.0	31.0	34.0	30.0	34.0
4	35.70946715551424	37.0	35.0	37.0	33.0	37.0
5	33.793153913714534	37.0	35.0	37.0	32.0	37.0
6	34.684883629093726	37.0	35.0	37.0	31.0	37.0
7	35.71439242482922	37.0	35.0	37.0	33.0	37.0
8	35.71882002879418	37.0	35.0	37.0	35.0	37.0
9	37.61205415893129	39.0	37.0	39.0	35.0	39.0
10	37.191755312315934	39.0	37.0	39.0	34.0	39.0
11	37.18985790369652	39.0	37.0	39.0	34.0	39.0
12	37.14120754738526	39.0	37.0	39.0	34.0	39.0
13	37.095236781180446	39.0	37.0	39.0	33.0	39.0
14	38.12283104023477	40.0	37.0	41.0	33.0	41.0
15	38.22706290851617	40.0	37.0	41.0	33.0	41.0
16	38.2402343880096	40.0	37.0	41.0	33.0	41.0
17	38.224504166401026	40.0	37.0	41.0	33.0	41.0
18	38.096430987708715	40.0	37.0	41.0	33.0	41.0
19	37.921118614682364	40.0	37.0	41.0	34.0	41.0
20	37.853460867612455	40.0	35.0	41.0	33.0	41.0
21	37.74953920756071	39.0	35.0	41.0	33.0	41.0
22	37.83329447800804	40.0	35.0	41.0	34.0	41.0
23	37.85281666217848	40.0	35.0	41.0	34.0	41.0
24	37.790462904900245	40.0	35.0	41.0	34.0	41.0
25	37.59604227586266	39.0	35.0	41.0	33.0	41.0
26	37.62638844350642	39.0	35.0	41.0	33.0	41.0
27	37.61627146836019	39.0	35.0	41.0	33.0	41.0
28	37.499197359994746	39.0	35.0	41.0	33.0	41.0
29	37.416045377944954	39.0	35.0	41.0	33.0	41.0
30	37.21045059172695	39.0	35.0	41.0	33.0	41.0
31	37.08664325197187	39.0	35.0	41.0	32.0	41.0
32	36.89689001976388	39.0	35.0	41.0	32.0	41.0
33	36.66903922040675	39.0	35.0	41.0	31.0	41.0
34	36.363212919887296	39.0	35.0	41.0	30.0	41.0
35	36.18893298836148	39.0	35.0	41.0	30.0	41.0
36	36.02306008048286	39.0	35.0	41.0	29.0	41.0
37	35.89995594281892	39.0	35.0	41.0	29.0	41.0
38	35.76001016264998	39.0	35.0	41.0	28.0	41.0
39	35.69632993214433	39.0	35.0	41.0	27.0	41.0
40	35.58704147940779	39.0	35.0	41.0	27.0	41.0
41	35.44708142585405	38.0	35.0	41.0	26.0	41.0
42	35.43625649082174	38.0	35.0	41.0	26.0	41.0
43	35.383648600684936	38.0	35.0	40.0	26.0	41.0
44	35.25219548447198	38.0	35.0	40.0	25.0	41.0
45	35.22306255453623	38.0	35.0	40.0	25.0	41.0
46	35.152061029187166	38.0	35.0	40.0	25.0	41.0
47	35.0882323554796	38.0	34.0	40.0	25.0	41.0
48	35.02312002869902	38.0	34.0	40.0	25.0	41.0
49	34.92975686716332	38.0	34.0	40.0	24.0	41.0
50	34.824565827423356	37.0	34.0	40.0	24.0	41.0
51	33.66476481743865	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	20.0
10	16.0
11	25.0
12	30.0
13	34.0
14	39.0
15	61.0
16	107.0
17	199.0
18	356.0
19	707.0
20	1154.0
21	1995.0
22	3015.0
23	4385.0
24	6603.0
25	10633.0
26	15406.0
27	17977.0
28	18224.0
29	17798.0
30	18940.0
31	22809.0
32	29331.0
33	41031.0
34	67704.0
35	79308.0
36	85682.0
37	123543.0
38	200814.0
39	282793.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.087929759721845	17.606600774378702	20.15982384740039	20.145645618499067
2	36.49951898693224	24.180350877860743	22.612657447328832	16.707472687878187
3	29.643346176207793	24.236778325770025	30.613460563113577	15.506414934908609
4	26.27996578193884	23.624259806053246	31.89587660944308	18.19989780256483
5	23.360297343152155	30.863720576606685	27.706162391153544	18.069819689087616
6	23.63196743384524	32.06135271722426	29.407264391616007	14.899415457314491
7	74.0353808662422	5.05858272901408	15.931476334252222	4.974560070491489
8	74.80604848954285	5.445772080688395	14.651344029490716	5.096835400278046
9	69.3393421111478	5.990825068250568	16.561408383424983	8.108424437176648
10	36.76985689504399	23.47362801846405	23.594095386175944	16.162419700316015
11	28.3435166004223	23.793256682085094	29.230940511386834	18.63228620610577
12	24.994314435054672	21.231469578183418	33.187522777943244	20.58669320881867
13	23.765946939167787	21.606288663031076	35.98253698947671	18.645227408324427
14	20.15316293449373	24.699426305086938	34.98359036527495	20.163820395144384
15	19.070764587161378	23.278843893893562	37.70143314298981	19.948958375955247
16	22.48267448975028	24.013352275700896	33.166398168439265	20.33757506610956
17	22.40959475957435	24.13981446502878	32.14737364961886	21.30321712577802
18	23.674787588245202	23.278368114400227	32.36461456627466	20.682229731079914
19	22.40331447026235	25.843200207059237	30.072499279194066	21.680986043484342
20	23.3459288024535	26.163685273768277	30.82375509916672	19.666630824611502
21	22.679171420496772	26.56714628411458	32.0245273844403	18.729154910948353
22	21.438814281377898	22.741308222326047	32.968378743314105	22.851498752981946
23	20.679375054119916	25.34639126012102	32.4584382823599	21.51579540339916
24	21.57926438780977	24.32822314438861	32.24671640782677	21.84579605997486
25	22.55565906402755	26.041885723475055	29.691399905034416	21.71105530746298
26	20.565854067010687	24.560974472527064	30.83431740391871	24.03885405654354
27	21.94437757099344	24.171406223386086	31.150425299289093	22.73379090633139
28	20.074469006296468	25.585042253976802	31.253764605240995	23.086724134485735
29	21.141927877538162	26.78124705611438	30.313624326415184	21.763200739932266
30	22.753963956848704	26.739473616599756	30.71289847722015	19.793663949331386
31	23.114890280491043	26.01657425442975	29.926530130639534	20.942005334439678
32	23.54442400707199	27.47388684250842	29.25101840600548	19.730670744414113
33	24.644901975150987	26.07595153519769	27.958896458011985	21.320250031639336
34	24.401302874564543	25.962525703987126	30.134065145631343	19.502106275816985
35	24.35715053758325	27.75002926043884	27.988204474801293	19.90461572717662
36	26.79647199990104	27.41803032999114	27.0915504416661	18.69394722844172
37	24.673734212446963	27.698169295665554	27.884103921660053	19.74399257022743
38	25.11868319461189	25.92788895687249	28.180324234209113	20.7731036143065
39	26.03931651421106	24.731874466532243	26.975174777596877	22.25363424165982
40	27.168721875484703	23.599233804703935	29.202108274090854	20.029936045720508
41	24.25952058555134	24.909911152937415	28.10810090712118	22.722467354390066
42	24.080722651956833	25.20441865931048	29.652195674783783	21.062663013948903
43	23.985281285594255	24.23163990724203	29.535249075322557	22.24782973184116
44	23.708377620474504	24.239442690932687	28.7116747723633	23.340504916229506
45	22.855209833029942	23.43242551434142	29.07098344572831	24.641381206900327
46	23.931423046948968	24.28892375823931	28.33247851617698	23.44717467863474
47	22.039343157862685	24.7784057009802	31.2084703974757	21.97378074368141
48	22.09700763245463	24.806096067492174	29.93166854916753	23.165227750885663
49	23.1266896119257	23.427001628117424	31.225122679742356	22.22118608021452
50	21.422352310908575	23.593238983087943	31.779691257171187	23.204717448832294
51	21.63378871774572	22.975391733045836	29.872291268399582	25.518528280808862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	597.0
1	983.0
2	1369.0
3	13041.0
4	24713.0
5	16062.0
6	7411.0
7	7152.0
8	6893.0
9	6996.0
10	7099.0
11	6988.0
12	6877.0
13	6598.0
14	6319.0
15	6098.5
16	5878.0
17	5439.5
18	5001.0
19	4776.5
20	4552.0
21	4677.5
22	4803.0
23	4942.5
24	5082.0
25	5619.0
26	7109.0
27	8062.0
28	8899.5
29	9737.0
30	11589.0
31	13441.0
32	15007.5
33	16574.0
34	20102.5
35	23631.0
36	25517.5
37	27404.0
38	29224.0
39	31044.0
40	34553.5
41	38063.0
42	42072.0
43	46081.0
44	51402.5
45	56724.0
46	79751.0
47	102778.0
48	99787.0
49	96796.0
50	97207.5
51	97619.0
52	87067.5
53	76516.0
54	69409.0
55	62302.0
56	57430.5
57	52559.0
58	49469.5
59	46380.0
60	43272.5
61	40165.0
62	35646.5
63	31128.0
64	27823.0
65	24518.0
66	21531.0
67	18544.0
68	16105.0
69	13666.0
70	11400.5
71	9135.0
72	7636.0
73	6137.0
74	5058.5
75	3277.5
76	2575.0
77	1959.5
78	1344.0
79	1005.0
80	666.0
81	511.5
82	357.0
83	256.0
84	155.0
85	94.5
86	34.0
87	28.5
88	23.0
89	17.5
90	12.0
91	8.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1050907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.408289880941325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20169033562648	25.91946671178763
2	9.361494640310003	6.629490318859502
3	3.885282505774313	4.127136277014209
4	2.160809246834996	3.060422407574081
5	1.5460483641425542	2.7371464323757344
6	1.2301336549076445	2.613415742716303
7	0.975748358596131	2.418470651241715
8	0.8474904870218001	2.400655106864643
9	0.7306857596114431	2.3285099869378008
>10	5.963745989210167	38.53616598316339
>50	0.06242833042529737	1.4922575502774293
>100	0.027984391129709626	1.800732815636227
>500	0.002690806837292284	0.7403012683107661
>1k	0.003228968204750741	2.9187115898882894
>5k	2.6908068372922845E-4	0.8460585923551613
>10k+	2.6908068372922845E-4	1.431058564997131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15020	1.4292415979720374	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	8880	0.8449843801592339	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT	3739	0.3557879051143441	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC	3382	0.32181724929037486	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	3345	0.3182964810397114	No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	3184	0.302976381354392	No Hit
GAACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT	2832	0.2694815050237557	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	2663	0.2534001581491036	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	2650	0.25216313146643804	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCT	2188	0.20820110628247793	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1859	0.17689481562117296	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTT	1823	0.17346920326917606	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT	1550	0.1474916429331996	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	1419	0.13502622020787758	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.709353919994823E-4	0.0	0.0	0.14301931569587034	0.0
2	5.709353919994823E-4	0.0	0.0	0.6520082176634089	0.0
3	5.709353919994823E-4	0.0	0.0	1.1371129890656357	0.0
4	5.709353919994823E-4	0.0	0.0	1.8052025536036966	0.0
5	5.709353919994823E-4	0.0	0.0	3.675586897794001	0.0
6	5.709353919994823E-4	0.0	0.0	5.282674870373877	0.0
7	5.709353919994823E-4	0.0	0.0	6.445289640282156	0.0
8	5.709353919994823E-4	0.0	0.0	7.959029676270117	0.0
9	5.709353919994823E-4	0.0	0.0	8.792500192690694	0.0
10	5.709353919994823E-4	0.0	0.0	10.272650196449353	0.0
11	5.709353919994823E-4	0.0	0.0	11.418993307685646	0.0
12	6.660912906660628E-4	0.0	0.0	12.621383243236556	0.0
13	6.660912906660628E-4	0.0	0.0	13.10991362699078	0.0
14	6.660912906660628E-4	0.0	0.0	13.398140844051852	0.0
15	6.660912906660628E-4	0.0	0.0	13.704733149555574	0.0
16	6.660912906660628E-4	0.0	0.0	14.160529904168495	0.0
17	6.660912906660628E-4	0.0	0.0	14.658004942397376	0.0
18	6.660912906660628E-4	0.0	0.0	15.208672127980877	0.0
19	6.660912906660628E-4	0.0	0.0	15.619460142524504	0.0
20	6.660912906660628E-4	0.0	0.0	15.97134665579352	0.0
21	6.660912906660628E-4	0.0	0.0	16.369669247611824	0.0
22	6.660912906660628E-4	0.0	0.0	16.82755943199541	0.0
23	6.660912906660628E-4	0.0	0.0	17.26803608692301	0.0
24	6.660912906660628E-4	0.0	0.0	17.65037248776533	0.0
25	6.660912906660628E-4	0.0	0.0	17.978850649962364	0.0
26	6.660912906660628E-4	0.0	0.0	18.312181762991397	0.0
27	6.660912906660628E-4	0.0	0.0	18.689760368900387	0.0
28	6.660912906660628E-4	0.0	0.0	19.014527451049428	0.0
29	6.660912906660628E-4	0.0	0.0	19.364130222750443	0.0
30	6.660912906660628E-4	0.0	0.0	19.798992679656717	0.0
31	8.564030879992235E-4	0.0	0.0	20.146026242093733	0.0
32	9.515589866658039E-4	0.0	0.0	20.5151359730214	0.0
33	9.515589866658039E-4	0.0	0.0	20.88776647219973	0.0
34	9.515589866658039E-4	0.0	0.0	21.262680712946054	0.0
35	9.515589866658039E-4	0.0	0.0	21.66823515306302	0.0
36	9.515589866658039E-4	0.0	0.0	22.008132023100046	0.0
37	9.515589866658039E-4	0.0	0.0	22.41654114017701	0.0
38	9.515589866658039E-4	0.0	0.0	22.849310167312616	0.0
39	0.0010467148853323842	0.0	0.0	23.454311371034734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7495	0.0	43.61908	1
TTAACGG	65	0.0	41.538464	2
CGGTCTA	65	0.0	41.538464	31
GTACGAG	60	3.6379788E-12	41.250004	1
CCATACG	100	0.0	40.500004	2
TTGCACG	50	1.0804797E-9	40.500004	1
CGTTATT	525	0.0	39.42857	1
GTTAACG	40	3.4578443E-7	39.375004	1
CTCACGA	75	0.0	39.000004	24
ACGTAAG	35	6.2477775E-6	38.571426	1
CGTAAGG	135	0.0	38.333332	2
TCCGAAC	125	0.0	37.800003	17
TCCGATC	30	1.1398552E-4	37.500004	14
GTTTTTT	9055	0.0	36.92435	2
TTACGGG	180	0.0	36.250004	3
CTAATCC	460	0.0	36.195656	11
AGGGCGA	790	0.0	36.170883	6
ACACCCG	25	0.0021070263	36.000004	12
AAGCGAC	25	0.0021070263	36.000004	15
CGTTTCT	420	0.0	35.892857	1
>>END_MODULE