SRR2935869.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	1923	0.620466689037454	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1697	0.5475465269352882	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	1683	0.5430293487519682	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	1676	0.5407707596603082	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1511	0.48753258821403683	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.2852275367182055	No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	842	0.2716760021682455	TruSeq Adapter, Index 20 (95% over 23bp)
ACTTTTTTTTCTTTTAATTTTTGTTTTATTTTTTGTAACCACTGTCTTAAT	507	0.16358638135308845	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	452	0.14584032420433135	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	397	0.1280942670555742	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	331	0.10679899847706564	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	327	0.10550837613897421	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	320	0.10324978704731422	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTACG	35	1.2080818E-7	45.000004	21
GGTATGC	35	1.2080818E-7	45.000004	8
TCTGATC	20	7.025304E-4	45.0	43
GTCGCTC	20	7.025304E-4	45.0	24
CGAACGG	20	7.025304E-4	45.0	2
CGAAACT	20	7.025304E-4	45.0	19
GCGCGAC	40	6.7866495E-9	45.0	9
ATTACGG	20	7.025304E-4	45.0	2
TACGGGT	20	7.025304E-4	45.0	4
CGCGACC	40	6.7866495E-9	45.0	10
ATCGTGA	20	7.025304E-4	45.0	30
GTAATCG	20	7.025304E-4	45.0	13
ATTCCCG	20	7.025304E-4	45.0	13
TTCCCGC	20	7.025304E-4	45.0	14
CCGTAAC	20	7.025304E-4	45.0	36
CTAGTAG	25	3.884191E-5	45.0	1
TGCGAAG	40	6.7866495E-9	45.0	1
ACGTGTG	25	3.884191E-5	45.0	40
ACTACGG	20	7.025304E-4	45.0	2
CGGGATC	40	6.7866495E-9	45.0	6