##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80877816783253	33.0	31.0	34.0	30.0	34.0
2	31.853769262538396	33.0	31.0	34.0	30.0	34.0
3	31.876119614878295	33.0	31.0	34.0	30.0	34.0
4	35.61896634056942	37.0	35.0	37.0	33.0	37.0
5	33.652135334658375	37.0	35.0	37.0	32.0	37.0
6	34.589501432590794	37.0	35.0	37.0	30.0	37.0
7	35.66388322449085	37.0	35.0	37.0	32.0	37.0
8	35.64483363878062	37.0	35.0	37.0	33.0	37.0
9	37.509979737229294	39.0	37.0	39.0	35.0	39.0
10	37.08997896285589	39.0	37.0	39.0	33.0	39.0
11	37.10646021011332	39.0	37.0	39.0	34.0	39.0
12	36.98885547611058	39.0	35.0	39.0	33.0	39.0
13	36.86024818667561	39.0	35.0	39.0	33.0	39.0
14	37.89797630417387	40.0	37.0	41.0	33.0	41.0
15	38.03170736429106	40.0	37.0	41.0	33.0	41.0
16	38.03624067525361	40.0	36.0	41.0	33.0	41.0
17	38.01293526238352	40.0	36.0	41.0	33.0	41.0
18	37.98409307968302	40.0	36.0	41.0	33.0	41.0
19	37.892394362561625	40.0	36.0	41.0	34.0	41.0
20	37.92032665651377	40.0	36.0	41.0	34.0	41.0
21	37.80725523347358	39.0	36.0	41.0	33.0	41.0
22	37.88595415709455	40.0	36.0	41.0	33.0	41.0
23	37.85757659843576	40.0	36.0	41.0	34.0	41.0
24	37.80426098964921	39.0	36.0	41.0	33.0	41.0
25	37.649170129836605	39.0	35.0	41.0	33.0	41.0
26	37.67160114607264	39.0	35.0	41.0	33.0	41.0
27	37.63381172401332	39.0	35.0	41.0	33.0	41.0
28	37.54855966547069	39.0	35.0	41.0	33.0	41.0
29	37.57580147647195	39.0	36.0	41.0	33.0	41.0
30	37.38249851578431	39.0	35.0	41.0	33.0	41.0
31	37.38782878604063	39.0	35.0	41.0	33.0	41.0
32	37.32111974394053	39.0	35.0	41.0	33.0	41.0
33	37.218866962649386	39.0	35.0	41.0	32.0	41.0
34	37.14000671123616	39.0	35.0	41.0	32.0	41.0
35	37.02182119718128	39.0	35.0	41.0	32.0	41.0
36	36.88638974213366	39.0	35.0	41.0	31.0	41.0
37	36.824652822591055	39.0	35.0	41.0	31.0	41.0
38	36.78186869208332	39.0	35.0	41.0	31.0	41.0
39	36.73912005368989	39.0	35.0	41.0	31.0	41.0
40	36.65356147234196	39.0	35.0	41.0	31.0	41.0
41	36.505181848687435	39.0	35.0	41.0	31.0	41.0
42	36.508411631088514	39.0	35.0	41.0	31.0	41.0
43	36.36985041687102	39.0	35.0	41.0	30.0	41.0
44	36.21726336439431	39.0	35.0	40.0	30.0	41.0
45	36.1849106889342	39.0	35.0	40.0	30.0	41.0
46	36.08041545133063	38.0	35.0	40.0	30.0	41.0
47	35.9960765080922	38.0	35.0	40.0	30.0	41.0
48	35.90873686791771	38.0	35.0	40.0	29.0	41.0
49	35.80980421259131	38.0	35.0	40.0	29.0	41.0
50	35.67134302160502	38.0	35.0	40.0	28.0	41.0
51	34.27831948065357	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	5.0
11	3.0
12	5.0
13	4.0
14	6.0
15	8.0
16	13.0
17	28.0
18	58.0
19	140.0
20	235.0
21	446.0
22	684.0
23	957.0
24	1393.0
25	1880.0
26	2533.0
27	3177.0
28	3591.0
29	4109.0
30	5220.0
31	6593.0
32	8687.0
33	12447.0
34	21919.0
35	28793.0
36	24143.0
37	36108.0
38	60469.0
39	86225.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.487106682842466	19.496141039209107	22.05350920213727	13.963243075811155
2	32.34105985906404	25.78018120337627	23.777780645827416	18.10097829173227
3	30.30994295449266	26.54939211687876	26.421620505407706	16.71904442322088
4	28.729898557084226	25.62595183397434	27.737732634676444	17.906416974264992
5	25.05743269404507	33.007343641103745	23.665496502413465	18.269727162437725
6	24.33823339614362	35.64827960042332	25.486564621460467	14.526922381972588
7	76.51712655842647	6.279200330399319	12.078611806613148	5.12506130456106
8	75.12906223380914	6.89934436385225	10.999974187553239	6.9716192147853695
9	69.62681655094086	7.072610412741024	13.779652048217649	9.520920988100462
10	39.91120518313931	23.8861929222271	20.491210861877597	15.711391032755994
11	31.91547714307839	24.800598848764874	24.59442193025477	18.689502077901963
12	28.09104049972897	20.828708603288508	30.845551224800598	20.23469967218193
13	23.850700807929584	25.226181564750522	32.656617020727396	18.266500606592498
14	19.70005936862755	29.01964327198575	29.57267494385793	21.707622415528768
15	17.73670013680597	24.281123357683075	39.20910663121757	18.773069874293384
16	20.411514932500452	23.15570067886735	33.886257453344	22.546526935288195
17	19.683603933816887	23.36574946439173	29.54008672982112	27.410559871970264
18	20.76611341989107	24.761234867453087	32.504000929248086	21.96865078340776
19	22.762060865749465	26.429364239436254	27.899705738106917	22.908869156707365
20	25.100023231202083	25.262964301386127	28.579541054696573	21.057471412715213
21	22.428757646937353	28.362393846312695	28.798624196587596	20.410224310162363
22	19.486784027257944	25.063885805735524	27.33215456493121	28.117175602075324
23	19.860096538550888	28.563730931054955	27.799682506904826	23.776490023489327
24	21.58114142639581	23.5996747631708	30.05698097622674	24.762202834206654
25	19.85557936036757	27.054993417826072	28.035221083606515	25.05420613819984
26	19.89946051986268	27.215998554502978	29.789176841072766	23.095364084561577
27	22.627836142587956	26.354185488242432	28.500490436488473	22.51748793268114
28	18.907939908623938	26.009266668387497	31.208538757388816	23.874254665599754
29	22.37390619756847	23.022121266874887	30.560968999251436	24.043003536305207
30	21.62469993030639	24.809633205131515	29.798211197439407	23.767455667122686
31	22.857889574352754	24.612813298572572	26.827198575152938	25.702098551921736
32	24.195296972199994	24.90642988048837	28.731189179422316	22.16708396788932
33	22.753026509382824	23.101817196252032	28.12298340259673	26.02217289176841
34	21.54080947833045	22.001884308613615	31.204344234790014	25.25296197826592
35	21.3339872486513	21.671162334477685	31.743824372112233	25.251026044758785
36	24.796081670581554	22.0906145943574	29.68850829870163	23.424795436359414
37	20.69577450246509	23.60870911953744	34.035969644562606	21.659546733434862
38	19.877197284530602	24.585387573888127	29.034162773289278	26.503252368291992
39	23.110851552618673	21.22912418368137	31.416651609406053	24.2433726542939
40	22.16998786815002	21.074894814279446	33.00798895227279	23.747128365297744
41	20.678028445316333	23.014054877261817	30.600010324978705	25.70790635244315
42	23.820693838568957	21.497250974419867	31.092382746960585	23.589672440050595
43	24.2998373815854	21.602436694974315	29.9647014790532	24.133024444387082
44	21.515642342737667	23.002439276218993	29.20678351100901	26.27513487003433
45	20.856779639141994	21.676970134999095	29.866936836942777	27.599313388916137
46	24.103985441780026	22.514906688004956	29.46458532304277	23.91652254717225
47	20.21017784775819	21.45466043726285	34.29764332361064	24.037518391368316
48	21.460145582199736	20.531865465527478	31.632185539867326	26.375803412405464
49	21.61889212978498	19.49904493946981	33.529077721277204	25.352985209468002
50	20.638019152835497	20.734815828192353	31.994850416871017	26.632314602101133
51	19.856547327121138	20.546062311246484	29.169355463204354	30.428034898428024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	354.0
2	540.0
3	1140.0
4	1740.0
5	1215.0
6	690.0
7	696.5
8	703.0
9	721.0
10	739.0
11	754.0
12	769.0
13	775.5
14	782.0
15	768.0
16	754.0
17	1023.5
18	1293.0
19	1080.5
20	868.0
21	920.0
22	972.0
23	1062.0
24	1152.0
25	1267.5
26	1581.5
27	1780.0
28	2087.5
29	2395.0
30	2661.5
31	2928.0
32	3669.5
33	4411.0
34	4773.0
35	5135.0
36	5869.0
37	6603.0
38	7644.5
39	8686.0
40	10625.0
41	12564.0
42	14802.5
43	17041.0
44	19916.0
45	22791.0
46	29315.5
47	35840.0
48	36875.0
49	37910.0
50	37487.5
51	37065.0
52	31230.5
53	25396.0
54	21657.5
55	17919.0
56	15713.0
57	13507.0
58	12574.5
59	11642.0
60	10981.0
61	10320.0
62	9355.5
63	8391.0
64	6866.0
65	5341.0
66	4310.5
67	3280.0
68	2801.5
69	2323.0
70	1835.0
71	1347.0
72	1115.0
73	883.0
74	836.5
75	691.0
76	592.0
77	436.5
78	281.0
79	194.0
80	107.0
81	82.5
82	58.0
83	48.0
84	38.0
85	22.0
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	309928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.941749205657715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29782115916225	23.181475170771478
2	13.557810471751864	9.203516056200833
3	5.09316430727109	5.186127167418083
4	2.4958218879591887	3.3885024233240775
5	1.4861626169994056	2.5221479412508883
6	1.0457786338241204	2.1297333668336185
7	0.7429873775900175	1.765280386319078
8	0.6085307183812381	1.6523677621787765
9	0.47905585566315545	1.4633994337578526
>10	6.032517730324019	40.86092062489474
>50	0.11154729813818698	2.530271114155662
>100	0.04083427878272916	2.491395873256811
>500	0.0029878740572728654	0.75485576458378
>1k	0.0049797900954547765	2.870006915054318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	1923	0.620466689037454	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1697	0.5475465269352882	TruSeq Adapter, Index 23 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	1683	0.5430293487519682	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	1676	0.5407707596603082	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1511	0.48753258821403683	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.2852275367182055	No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	842	0.2716760021682455	TruSeq Adapter, Index 20 (95% over 23bp)
ACTTTTTTTTCTTTTAATTTTTGTTTTATTTTTTGTAACCACTGTCTTAAT	507	0.16358638135308845	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	452	0.14584032420433135	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	397	0.1280942670555742	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	331	0.10679899847706564	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	327	0.10550837613897421	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	320	0.10324978704731422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2997470380217341	0.0
2	0.0	0.0	0.0	1.700394930435456	0.0
3	0.0	0.0	0.0	2.235357889574353	0.0
4	0.0	0.0	0.0	3.031026561007718	0.0
5	0.0	0.0	0.0	5.378345938411502	0.0
6	0.0	0.0	0.0	6.6670323429957925	0.0
7	0.0	0.0	0.0	8.077037247360677	0.0
8	0.0	0.0	0.0	10.440489403990604	0.0
9	0.0	0.0	0.0	11.383289021966393	0.0
10	0.0	0.0	0.0	13.713184997805943	0.0
11	0.0	0.0	0.0	15.864652435404352	0.0
12	0.0	0.0	0.0	18.21358509073075	0.0
13	0.0	0.0	0.0	19.003123306058182	0.0
14	0.0	0.0	0.0	19.328037479672698	0.0
15	0.0	0.0	0.0	19.949149479879196	0.0
16	0.0	0.0	0.0	21.048759711933094	0.0
17	0.0	0.0	0.0	22.276464211042565	0.0
18	0.0	0.0	0.0	23.765197078031026	0.0
19	0.0	0.0	0.0	24.63314060039751	0.0
20	0.0	0.0	0.0	25.4065460364988	0.0
21	0.0	0.0	0.0	26.313853540177075	0.0
22	0.0	0.0	0.0	27.21373996541132	0.0
23	0.0	0.0	0.0	28.170736429106114	0.0
24	0.0	0.0	0.0	28.898324772205157	0.0
25	0.0	0.0	0.0	29.549766397356805	0.0
26	0.0	0.0	0.0	30.16023076327405	0.0
27	0.0	0.0	0.0	30.724232725020006	0.0
28	0.0	0.0	0.0	31.320177589633722	0.0
29	0.0	0.0	0.0	31.93322320022715	0.0
30	0.0	0.0	0.0	32.61273586123229	0.0
31	0.0	0.0	0.0	33.30289615652668	0.0
32	0.0	0.0	0.0	33.90852068867608	0.0
33	0.0	0.0	0.0	34.46284298288635	0.0
34	0.0	0.0	0.0	35.02781291138587	0.0
35	0.0	0.0	0.0	35.67344673601611	0.0
36	0.0	0.0	0.0	36.24616039854418	0.0
37	0.0	0.0	0.0	36.84629978575669	0.0
38	0.0	0.0	0.0	37.414818925685964	0.0
39	0.0	0.0	0.0	37.97333574249503	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTACG	35	1.2080818E-7	45.000004	21
GGTATGC	35	1.2080818E-7	45.000004	8
TCTGATC	20	7.025304E-4	45.0	43
GTCGCTC	20	7.025304E-4	45.0	24
CGAACGG	20	7.025304E-4	45.0	2
CGAAACT	20	7.025304E-4	45.0	19
GCGCGAC	40	6.7866495E-9	45.0	9
ATTACGG	20	7.025304E-4	45.0	2
TACGGGT	20	7.025304E-4	45.0	4
CGCGACC	40	6.7866495E-9	45.0	10
ATCGTGA	20	7.025304E-4	45.0	30
GTAATCG	20	7.025304E-4	45.0	13
ATTCCCG	20	7.025304E-4	45.0	13
TTCCCGC	20	7.025304E-4	45.0	14
CCGTAAC	20	7.025304E-4	45.0	36
CTAGTAG	25	3.884191E-5	45.0	1
TGCGAAG	40	6.7866495E-9	45.0	1
ACGTGTG	25	3.884191E-5	45.0	40
ACTACGG	20	7.025304E-4	45.0	2
CGGGATC	40	6.7866495E-9	45.0	6
>>END_MODULE