SRR2935868.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935868.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421466
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	2597	0.6161825627690015	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	2349	0.5573403311299132	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	2212	0.5248347434905781	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1787	0.4239962416897211	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1186	0.28139873679015626	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	825	0.19574532702519304	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.18981365044867202	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATC	538	0.12764967992673193	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	487	0.11554905971062909	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	455	0.10795651369268221	TruSeq Adapter, Index 16 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	20	7.0281955E-4	45.000004	6
GGCGTAC	20	7.0281955E-4	45.000004	32
TCGGCTT	20	7.0281955E-4	45.000004	38
CGTTATT	20	7.0281955E-4	45.000004	1
ATCCGCA	20	7.0281955E-4	45.000004	18
TACGCTG	20	7.0281955E-4	45.000004	36
ACTAGGC	20	7.0281955E-4	45.000004	45
CTACGAA	65	0.0	45.000004	11
TATTACG	20	7.0281955E-4	45.000004	1
TTACGTT	20	7.0281955E-4	45.000004	28
CGTAAGT	20	7.0281955E-4	45.000004	14
TTCGGAA	20	7.0281955E-4	45.000004	14
CGCAATG	20	7.0281955E-4	45.000004	29
TCGACAG	20	7.0281955E-4	45.000004	34
ATTTGCG	20	7.0281955E-4	45.000004	12
GTACGTA	20	7.0281955E-4	45.000004	11
GTACGCT	20	7.0281955E-4	45.000004	35
AACACGT	20	7.0281955E-4	45.000004	41
GGTCGAA	25	3.8865877E-5	45.0	8
TCGATGG	25	3.8865877E-5	45.0	2