##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935867.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004191
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916795709182814	33.0	31.0	34.0	30.0	34.0
2	31.95883552033428	33.0	31.0	34.0	30.0	34.0
3	31.98375109914349	33.0	31.0	34.0	30.0	34.0
4	35.690941265157726	37.0	35.0	37.0	33.0	37.0
5	33.59804857840789	37.0	35.0	37.0	32.0	37.0
6	34.5655816473161	37.0	35.0	37.0	30.0	37.0
7	35.683920688394934	37.0	35.0	37.0	32.0	37.0
8	35.69519344427504	37.0	35.0	37.0	35.0	37.0
9	37.58272778784116	39.0	37.0	39.0	35.0	39.0
10	37.188300831216374	39.0	37.0	39.0	34.0	39.0
11	37.16695927368399	39.0	37.0	39.0	34.0	39.0
12	37.07355971125015	39.0	37.0	39.0	33.0	39.0
13	36.99797648056993	39.0	37.0	39.0	33.0	39.0
14	37.922452003652694	40.0	37.0	41.0	33.0	41.0
15	38.04606792930827	40.0	37.0	41.0	33.0	41.0
16	38.09449098826817	40.0	37.0	41.0	33.0	41.0
17	38.108430567491645	40.0	37.0	41.0	33.0	41.0
18	38.0506915517068	40.0	37.0	41.0	33.0	41.0
19	37.944535451921	40.0	37.0	41.0	33.0	41.0
20	37.96636197695458	40.0	36.0	41.0	33.0	41.0
21	37.85505147925046	40.0	36.0	41.0	33.0	41.0
22	37.96070169917874	40.0	36.0	41.0	34.0	41.0
23	37.960889910385575	40.0	36.0	41.0	34.0	41.0
24	37.88511548101905	40.0	36.0	41.0	33.0	41.0
25	37.690942260984215	40.0	36.0	41.0	33.0	41.0
26	37.730498480866686	40.0	36.0	41.0	33.0	41.0
27	37.7362075541406	40.0	36.0	41.0	33.0	41.0
28	37.6177510055358	40.0	35.0	41.0	33.0	41.0
29	37.6128535308522	40.0	36.0	41.0	33.0	41.0
30	37.43248644929102	39.0	35.0	41.0	33.0	41.0
31	37.370238331154134	39.0	35.0	41.0	33.0	41.0
32	37.329915324873454	40.0	35.0	41.0	33.0	41.0
33	37.25418272021956	40.0	35.0	41.0	32.0	41.0
34	37.11983975160104	40.0	35.0	41.0	32.0	41.0
35	37.04502828645148	40.0	35.0	41.0	32.0	41.0
36	36.92119427479434	39.0	35.0	41.0	31.0	41.0
37	36.85236872268324	39.0	35.0	41.0	31.0	41.0
38	36.665308691274866	39.0	35.0	41.0	31.0	41.0
39	36.59977334989061	39.0	35.0	41.0	31.0	41.0
40	36.3849805465295	39.0	35.0	41.0	30.0	41.0
41	36.208479263407064	39.0	35.0	41.0	30.0	41.0
42	36.3121258804351	39.0	35.0	41.0	30.0	41.0
43	36.295428857657555	39.0	35.0	41.0	30.0	41.0
44	36.17479144903709	38.0	35.0	40.0	30.0	41.0
45	36.107350095748714	38.0	35.0	40.0	30.0	41.0
46	35.99021998803016	38.0	35.0	40.0	30.0	41.0
47	35.885698039516384	38.0	35.0	40.0	30.0	41.0
48	35.797246738917195	38.0	35.0	40.0	30.0	41.0
49	35.72335740909847	38.0	35.0	40.0	29.0	41.0
50	35.59844192987191	37.0	35.0	40.0	29.0	41.0
51	34.30017695836748	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	25.0
10	25.0
11	18.0
12	28.0
13	25.0
14	38.0
15	71.0
16	82.0
17	164.0
18	285.0
19	552.0
20	904.0
21	1544.0
22	2285.0
23	3338.0
24	4631.0
25	6536.0
26	8704.0
27	10543.0
28	11678.0
29	13389.0
30	16268.0
31	21099.0
32	28101.0
33	40726.0
34	67454.0
35	77935.0
36	84971.0
37	126816.0
38	202260.0
39	273544.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.646088244168695	18.490506288146378	22.514441973688275	14.348963493996662
2	32.1594198713193	25.096122152060712	24.85453464530154	17.889923331318442
3	31.936255154646876	25.319087703434906	26.115948061673528	16.628709080244693
4	27.879556777545307	24.90094015979032	27.559697308579743	19.65980575408463
5	24.81679282128599	32.44084043772549	23.79806232081347	18.944304420175044
6	25.220899211405	34.04133277434273	25.275171755174064	15.462596259078204
7	79.60069349356846	5.312037251877382	10.448709458658762	4.638559795895402
8	80.00689111931894	5.543865659023035	9.340055826033096	5.109187395624936
9	74.18240155508265	6.5000582558497335	11.239893605897683	8.077646583169935
10	40.70669822772759	26.969271781961794	17.306468590138728	15.01756140017188
11	31.144174763565896	23.556574396703418	24.097706512008173	21.201544327722516
12	27.071144832009047	20.218563998283194	29.94778881706767	22.762502352640084
13	27.055311190799358	20.656428906453055	33.46713921953094	18.821120683216638
14	22.576681129386742	25.96189370348868	30.82740235672297	20.634022810401607
15	19.479162828585398	25.557886896018783	33.75284183984919	21.210108435546623
16	25.341294634188117	24.21850026538776	28.901772670736943	21.538432429687184
17	25.63197638696224	24.402230253009638	26.79161633593609	23.174177024092028
18	24.220392335720994	23.675675245048	28.754191184744737	23.349741234486267
19	25.193215234950323	24.58566149268416	25.721202440571567	24.49992083179395
20	26.4460645434982	26.3728712963968	25.679776058538668	21.501288101566335
21	24.330431162995882	26.776977686515814	27.35445746874848	21.53813368173983
22	24.46795480142722	20.915742124755152	27.836636655775642	26.779666418041987
23	24.583570257052692	23.976713593330352	28.181391787020598	23.25832436259636
24	23.671194025837714	23.24657361000049	27.719627043062523	25.362605321099274
25	22.965053460945178	24.9099025982109	25.326058488873134	26.798985451970793
26	21.376511042222045	23.089830520289468	27.68616727295903	27.84749116452946
27	22.822949020654438	22.639916111576383	28.45066327023445	26.086471597534732
28	19.710792070432817	25.83801288798645	27.44448018355074	27.006714858029994
29	23.3760310538533	24.954316459717322	26.698008645765597	24.97164384066378
30	24.27008407763065	24.180559276073975	28.666060540275705	22.88329610601967
31	23.069913990465956	25.1698133124077	26.214335719001664	25.54593697812468
32	23.690712225064754	23.47800368654967	28.020964139292225	24.810319949093348
33	22.591618526754374	22.82643441337355	29.5337241620369	25.04822289783517
34	20.89911182235252	23.48288323635643	29.327787243661813	26.290217697629238
35	21.62118561110386	23.93180181857834	28.76375111906002	25.683261451257778
36	23.99194973864534	24.739516685570774	27.468678767286303	23.799854808497585
37	22.93448158766609	24.661344306013497	28.716051030132718	23.688123076187697
38	25.214625504510597	26.424355525990574	25.20237683866914	23.158642130829694
39	24.864990823458886	22.710420627151606	26.058090542536235	26.366498006853277
40	25.31321232713697	22.044710617800796	29.389528486114692	23.25254856894754
41	20.99978988061036	23.78093410516525	28.14275371916299	27.0765222950614
42	22.59819098159613	23.527595845810207	29.74752810969228	24.12668506290138
43	22.111829323306022	21.767074192061074	30.785478061444486	25.335618423188418
44	22.421630944710717	22.268871160964398	30.542396814948553	24.76710107937633
45	23.058959899063026	22.169487677145085	28.053627248202783	26.71792517558911
46	25.656971631890745	22.726353851010415	26.282848581594536	25.3338259355043
47	21.835188724057474	24.4922529678119	28.722125571728885	24.95043273640174
48	20.46493147220001	25.62839141159401	27.123624888094	26.78305222811198
49	21.709814168818482	21.907087396720346	28.630708699838976	27.752389734622195
50	20.712693103204472	23.332812184136284	28.732083836640637	27.222410876018603
51	21.015922269767405	22.697972796011914	26.94198613610359	29.34411879811709
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	556.0
1	937.5
2	1319.0
3	4829.0
4	8339.0
5	5434.5
6	2530.0
7	2419.5
8	2309.0
9	2344.0
10	2379.0
11	2375.5
12	2372.0
13	2335.5
14	2299.0
15	2235.0
16	2171.0
17	2263.0
18	2355.0
19	2283.0
20	2211.0
21	2227.0
22	2243.0
23	2531.0
24	2819.0
25	3161.5
26	4144.5
27	4785.0
28	5705.5
29	6626.0
30	8105.0
31	9584.0
32	11191.0
33	12798.0
34	15000.0
35	17202.0
36	18699.0
37	20196.0
38	23318.5
39	26441.0
40	29168.5
41	31896.0
42	36182.0
43	40468.0
44	46978.0
45	53488.0
46	72032.5
47	90577.0
48	91643.5
49	92710.0
50	95788.5
51	98867.0
52	93133.0
53	87399.0
54	82251.5
55	77104.0
56	71759.0
57	66414.0
58	60746.0
59	55078.0
60	49623.0
61	44168.0
62	40436.5
63	36705.0
64	32299.0
65	27893.0
66	24618.5
67	21344.0
68	18867.0
69	16390.0
70	13512.5
71	10635.0
72	9103.0
73	7571.0
74	6269.0
75	3914.5
76	2862.0
77	2133.0
78	1404.0
79	976.5
80	549.0
81	450.5
82	352.0
83	258.0
84	164.0
85	119.5
86	75.0
87	51.0
88	27.0
89	24.0
90	21.0
91	16.0
92	11.0
93	11.0
94	11.0
95	6.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1004191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.607464426284476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54971185887753	22.615049722903763
2	10.477176666427626	6.623139775440179
3	4.018047395443946	3.810008703438586
4	2.086955623446709	2.6385350250930477
5	1.362836751626656	2.153790707293631
6	1.0080851822644832	1.9117809922253493
7	0.8036752320937774	1.7781495416082993
8	0.6880724439918865	1.7398580236944134
9	0.5889096080610892	1.675254553837921
>10	7.263245639488003	46.83616396855472
>50	0.1075842657562846	2.1731871646630774
>100	0.038400133577596786	2.2065827578946164
>500	0.003808277710117889	0.7974512648927568
>1k	0.002856208282588417	1.8311286591971416
>5k	6.347129516863148E-4	1.2099191392624444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	7033	0.7003647712437177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5029	0.5008011424121507	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	3773	0.37572533512051	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	3480	0.34654761892906827	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2562	0.2551307470391589	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	2249	0.22396137786536624	No Hit
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1386	0.13802155167692204	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT	1386	0.13802155167692204	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1313	0.13075201829134098	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	1065	0.106055521310189	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	1041	0.10366553773136784	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.970785438228385E-4	0.0	0.0	0.11053674052047867	0.0
2	6.970785438228385E-4	0.0	0.0	0.6069562463714572	0.0
3	6.970785438228385E-4	0.0	0.0	0.8996296521279319	0.0
4	6.970785438228385E-4	0.0	0.0	1.4362805482224	0.0
5	6.970785438228385E-4	0.0	0.0	3.007595168648195	0.0
6	6.970785438228385E-4	0.0	0.0	4.160065166885582	0.0
7	6.970785438228385E-4	0.0	0.0	5.154696666271655	0.0
8	6.970785438228385E-4	0.0	0.0	6.680402433401614	0.0
9	6.970785438228385E-4	0.0	0.0	7.499170974446097	0.0
10	6.970785438228385E-4	0.0	0.0	9.031250031119578	0.0
11	6.970785438228385E-4	0.0	0.0	10.273145248264523	0.0
12	7.966611929403868E-4	0.0	0.0	11.714604094241036	0.0
13	7.966611929403868E-4	0.0	0.0	12.252748730072268	0.0
14	7.966611929403868E-4	0.0	0.0	12.552691669214322	0.0
15	7.966611929403868E-4	0.0	0.0	12.90700673477456	0.0
16	7.966611929403868E-4	0.0	0.0	13.448736345974023	0.0
17	7.966611929403868E-4	0.0	0.0	14.068439171432527	0.0
18	7.966611929403868E-4	0.0	0.0	14.751177813782437	0.0
19	8.962438420579352E-4	0.0	0.0	15.244908588107242	0.0
20	8.962438420579352E-4	0.0	0.0	15.6787901903124	0.0
21	9.958264911754835E-4	0.0	0.0	16.18277797749631	0.0
22	9.958264911754835E-4	0.0	0.0	16.757768193501036	0.0
23	9.958264911754835E-4	0.0	0.0	17.283166250245223	0.0
24	9.958264911754835E-4	0.0	0.0	17.722823646099197	0.0
25	0.0010954091402930318	0.0	0.0	18.104324774868527	0.0
26	0.0010954091402930318	0.0	0.0	18.487419226023736	0.0
27	0.0010954091402930318	0.0	0.0	18.923690811807713	0.0
28	0.0011949917894105802	0.0	0.0	19.313656465752032	0.0
29	0.0011949917894105802	0.0	0.0	19.73050943495809	0.0
30	0.0011949917894105802	0.0	0.0	20.31018003547134	0.0
31	0.0012945744385281287	0.0	0.0	20.757903625903836	0.0
32	0.0012945744385281287	0.0	0.0	21.2542235491057	0.0
33	0.0012945744385281287	0.0	0.0	21.725946557975526	0.0
34	0.0012945744385281287	0.0	0.0	22.19527958326653	0.0
35	0.001394157087645677	0.0	0.0	22.708827304765727	0.0
36	0.001394157087645677	0.0	0.0	23.1388251836553	0.0
37	0.0014937397367632253	0.0	0.0	23.57390177764987	0.0
38	0.0014937397367632253	0.0	0.0	24.08525868086848	0.0
39	0.0014937397367632253	0.0	0.0	24.892276469317093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	25	3.890453E-5	45.0	34
CTCGTAC	50	2.1827873E-11	45.0	29
ATTCACG	20	7.032862E-4	45.0	22
TCGTATC	25	3.890453E-5	45.0	33
CGGTCTA	90	0.0	45.0	31
ATAACGG	60	0.0	44.999996	2
CGTTTTT	2375	0.0	42.06316	1
CGTAAGG	180	0.0	41.25	2
ACGTAAG	60	3.6379788E-12	41.249996	1
TTGTACG	45	1.9279469E-8	40.0	1
GCGATAT	90	0.0	40.0	9
TTTAGCG	85	0.0	39.705883	1
CCCTCGT	35	6.247501E-6	38.571426	27
CACGGGT	70	0.0	38.571426	4
TACACGG	100	0.0	38.25	2
CGGGTAC	130	0.0	38.076927	6
ACGATAG	30	1.1398192E-4	37.499996	1
TCGTACG	60	1.546141E-10	37.499996	30
TAGACGG	170	0.0	37.058823	2
ATGGGAC	770	0.0	36.818184	5
>>END_MODULE