SRR2935866.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935866.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553177
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	2350	0.42481881929292076	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	2073	0.374744430806053	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2046	0.36986353373332587	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	1791	0.32376617249090256	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1502	0.2715224964161561	Illumina Paired End PCR Primer 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.22289429965454094	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	1100	0.19885136222221822	TruSeq Adapter, Index 1 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	55	1.8189894E-12	45.000004	14
CGTAAGG	55	1.8189894E-12	45.000004	2
TCACGGA	20	7.0301106E-4	45.0	25
AACGTTC	20	7.0301106E-4	45.0	21
TTAGGCG	20	7.0301106E-4	45.0	1
GACGTAG	20	7.0301106E-4	45.0	1
TTCGTAT	20	7.0301106E-4	45.0	40
CGAATAA	20	7.0301106E-4	45.0	36
TTTGCGT	20	7.0301106E-4	45.0	12
CCAACGA	20	7.0301106E-4	45.0	24
GCCCGTC	25	3.888172E-5	45.0	33
ATCGCAA	20	7.0301106E-4	45.0	19
GTACGAG	45	3.8380676E-10	45.0	1
ACGATAG	30	2.1634532E-6	44.999996	1
TCGTGCG	30	2.1634532E-6	44.999996	1
TCGTAAG	30	2.1634532E-6	44.999996	1
AATCGTT	30	2.1634532E-6	44.999996	22
TAATCGT	30	2.1634532E-6	44.999996	21
GTTACGG	70	0.0	41.785713	2
TAGCGCG	40	3.453879E-7	39.375	1