##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935865.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1209926
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.904975180300283	33.0	31.0	34.0	30.0	34.0
2	31.975902658509693	33.0	31.0	34.0	30.0	34.0
3	31.890709018568078	33.0	31.0	34.0	30.0	34.0
4	35.6709997140321	37.0	35.0	37.0	33.0	37.0
5	33.513204113309406	37.0	35.0	37.0	32.0	37.0
6	34.54157692288619	37.0	35.0	37.0	30.0	37.0
7	35.69368126645762	37.0	35.0	37.0	32.0	37.0
8	35.72080937181282	37.0	35.0	37.0	35.0	37.0
9	37.63588516983683	39.0	37.0	39.0	35.0	39.0
10	37.18456583295177	39.0	37.0	39.0	34.0	39.0
11	37.214209794648596	39.0	37.0	39.0	34.0	39.0
12	37.30393924917722	39.0	37.0	39.0	34.0	39.0
13	37.34508143473237	39.0	37.0	39.0	34.0	39.0
14	38.50674173461848	40.0	38.0	41.0	34.0	41.0
15	38.54841039865248	40.0	38.0	41.0	34.0	41.0
16	38.52376674275948	40.0	38.0	41.0	34.0	41.0
17	38.47609109978627	40.0	38.0	41.0	34.0	41.0
18	38.30349211439377	40.0	38.0	41.0	34.0	41.0
19	38.063689845494686	40.0	37.0	41.0	34.0	41.0
20	37.9498919768647	40.0	36.0	41.0	34.0	41.0
21	37.86177336465205	39.0	36.0	41.0	34.0	41.0
22	37.94930268462699	40.0	36.0	41.0	34.0	41.0
23	37.925096245555515	40.0	36.0	41.0	34.0	41.0
24	37.848046078851105	40.0	35.0	41.0	34.0	41.0
25	37.659220481252575	39.0	35.0	41.0	33.0	41.0
26	37.698034425245844	39.0	35.0	41.0	33.0	41.0
27	37.69071496934523	40.0	35.0	41.0	33.0	41.0
28	37.54174966072305	39.0	35.0	41.0	33.0	41.0
29	37.50203648818192	40.0	35.0	41.0	33.0	41.0
30	37.280982473308285	39.0	35.0	41.0	33.0	41.0
31	37.107777665741544	39.0	35.0	41.0	32.0	41.0
32	36.7954593917314	39.0	35.0	41.0	31.0	41.0
33	36.414220373807986	39.0	35.0	41.0	30.0	41.0
34	36.05099650722441	39.0	35.0	41.0	27.0	41.0
35	35.84093159416361	39.0	35.0	41.0	25.0	41.0
36	35.66912604572511	39.0	35.0	41.0	24.0	41.0
37	35.61297054530608	39.0	35.0	41.0	23.0	41.0
38	35.4431006524366	39.0	35.0	41.0	23.0	41.0
39	35.345802139965585	39.0	35.0	41.0	23.0	41.0
40	35.23781041154583	39.0	35.0	41.0	22.0	41.0
41	35.109434791879835	39.0	35.0	41.0	21.0	41.0
42	35.10091360959265	39.0	35.0	41.0	21.0	41.0
43	35.012549527822365	39.0	35.0	41.0	21.0	41.0
44	34.86927051737048	39.0	35.0	41.0	21.0	41.0
45	34.789293725401386	39.0	34.0	41.0	20.0	41.0
46	34.650649709155765	38.0	34.0	40.0	20.0	41.0
47	34.53273423333328	38.0	34.0	40.0	20.0	41.0
48	34.4516408441508	38.0	34.0	40.0	20.0	41.0
49	34.33761320940289	38.0	34.0	40.0	19.0	41.0
50	34.22251608776074	38.0	34.0	40.0	18.0	41.0
51	32.947969545244916	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	11.0
10	7.0
11	14.0
12	16.0
13	25.0
14	40.0
15	47.0
16	82.0
17	197.0
18	394.0
19	768.0
20	1416.0
21	2426.0
22	3820.0
23	6091.0
24	9932.0
25	17280.0
26	25309.0
27	27263.0
28	24785.0
29	22735.0
30	23180.0
31	26206.0
32	31390.0
33	40522.0
34	60787.0
35	76595.0
36	89574.0
37	136084.0
38	231852.0
39	350874.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22475258817481	17.080052829677186	20.501667044100216	21.193527538047782
2	38.088279779093924	23.811042989405966	21.884644184850973	16.216033046649134
3	28.168582210812893	24.344464041602546	32.75117651823335	14.735777229351216
4	23.957002329068057	22.812800121660334	35.743673580037125	17.486523969234483
5	22.5225344359903	31.071569666244052	29.936954822030437	16.46894107573521
6	21.801663903412276	32.11055882756466	31.85269181751611	14.23508545150695
7	72.28508189757059	4.3363809026337155	18.962729952079712	4.415807247715977
8	72.87577917988372	5.1534556658836985	17.02228070146439	4.948484452768185
9	66.94938368131605	6.0178060476425825	19.009592322175077	8.023217948866295
10	30.829654044958122	28.91358645074161	25.97365458714004	14.283104917160221
11	23.5761525911502	22.988678646462677	35.50960967860844	17.925559083778676
12	20.112221739180743	21.14550807239451	37.97670270743831	20.76556748098644
13	20.359757538890808	22.265080674355293	40.686207255650345	16.688954531103555
14	18.5003876270119	25.774964749910325	37.30451283797522	18.42013478510256
15	17.311141342528387	24.94359159155188	40.18369718478651	17.561569881133227
16	20.03221684631953	25.86389580850399	35.62449273757238	18.479394607604103
17	19.799640639179586	26.170856730081017	35.453325244684386	18.576177386055015
18	21.040708274720934	23.910222608655403	35.848473377710704	19.20059573891296
19	18.79544699427899	27.081738883204427	33.927364152849016	20.195449969667568
20	20.199747753168378	26.171931175956214	34.996851047088825	18.631470023786576
21	19.262996249357396	28.208749956608912	35.27339688542936	17.254856908604328
22	19.915680793701434	22.942560123511686	35.71491149045479	21.426847592332095
23	17.911178038987508	27.22769822286652	35.43729120623906	19.42383253190691
24	18.68535761691211	24.794574213629595	35.843018498651986	20.677049670806312
25	19.016286946474413	28.212303892965355	33.274844907870396	19.496564252689836
26	19.595247973843026	25.649006633463532	33.927281503166306	20.82846388952713
27	21.0939346703848	24.787301041551302	33.02210217814974	21.09666210991416
28	18.738914611306807	25.99572205242304	33.996872536006336	21.268490800263816
29	21.01988055467855	24.185198103024483	32.6950573836747	22.09986395862226
30	20.79780085724251	24.617703892634758	32.95432943832929	21.630165811793447
31	20.36157583191038	25.65528800934933	30.95503361362596	23.02810254511433
32	20.668371454121985	26.33293275787114	30.858498784223166	22.1401970037837
33	18.768089949302684	26.862056026566915	29.545360625360562	24.824493398769842
34	18.962977901127836	27.665576241852808	30.82775310225584	22.543692754763516
35	18.099040767782494	26.944127161495828	30.935280339458775	24.021551731262903
36	18.949836601577285	30.81849633779256	28.92664510060946	21.305021960020696
37	17.80332020305374	28.15858159920524	30.041010772559645	23.997087425181373
38	18.30954950963943	29.62883680489551	30.21333536100555	21.848278324459514
39	19.226795688331354	27.32390245353848	29.587429313858866	23.861872544271304
40	20.515304241747014	26.401118746105134	32.30065309779276	20.78292391435509
41	18.636759603479884	27.650864598330806	30.60063177417462	23.11174402401469
42	20.589275707770557	26.41822723042566	32.17014924879704	20.822347813006743
43	20.531586229240467	26.580964455677453	30.75832736878123	22.12912194630085
44	20.924337521468257	25.914477414321208	30.29391880164572	22.86726626256482
45	20.003454756737188	24.535880706753968	30.61848410563952	24.842180430869327
46	21.741908182814488	25.360641890495785	29.254103143498035	23.6433467831917
47	19.068438896263075	25.30758079419733	32.638194401971695	22.9857859075679
48	19.428130315407717	25.731656316171403	30.96296798316591	23.877245385254966
49	20.22983223767404	24.313470410587094	32.52455108824837	22.932146263490495
50	18.83578003944043	24.53455831183064	32.88978003613444	23.73988161259449
51	19.070091889917233	23.475650576977436	30.940900517882913	26.513357015222418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	358.0
1	681.5
2	1005.0
3	16500.5
4	31996.0
5	21871.0
6	11746.0
7	11362.5
8	10979.0
9	11208.0
10	11437.0
11	11129.0
12	10821.0
13	10513.0
14	10205.0
15	9788.5
16	9372.0
17	8862.5
18	8353.0
19	7935.5
20	7518.0
21	7475.5
22	7433.0
23	7584.5
24	7736.0
25	8536.0
26	10269.0
27	11202.0
28	13174.0
29	15146.0
30	17814.0
31	20482.0
32	22982.0
33	25482.0
34	29187.0
35	32892.0
36	36032.0
37	39172.0
38	42827.0
39	46482.0
40	50294.0
41	54106.0
42	57913.0
43	61720.0
44	70092.0
45	78464.0
46	107375.5
47	136287.0
48	129202.5
49	122118.0
50	120348.5
51	118579.0
52	103196.0
53	87813.0
54	76273.5
55	64734.0
56	55723.5
57	46713.0
58	40847.0
59	34981.0
60	29816.5
61	24652.0
62	21186.5
63	17721.0
64	14557.0
65	11393.0
66	9522.5
67	7652.0
68	6190.0
69	4728.0
70	3882.5
71	3037.0
72	2750.5
73	2464.0
74	2028.5
75	1296.5
76	1000.0
77	777.0
78	554.0
79	406.5
80	259.0
81	178.0
82	97.0
83	69.0
84	41.0
85	29.5
86	18.0
87	12.0
88	6.0
89	7.0
90	8.0
91	12.5
92	17.0
93	11.5
94	6.0
95	9.0
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1209926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.03493709482782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1807229609958	28.956398544313206
2	9.371550353823011	7.316357570849854
3	3.969432749160031	4.648396729968338
4	2.3508980160376263	3.6706862468953707
5	1.6439392368283785	3.208553234865751
6	1.2399917433587035	2.9041799820067724
7	0.9928121576553425	2.7128052084739602
8	0.8557249652374777	2.6722536150814933
9	0.6833315366950122	2.4006423194821647
>10	4.604018935824528	31.282714291481785
>50	0.07706311795861233	1.9723758201450474
>100	0.025536728795163124	1.7670827494131605
>500	0.001082064702242295	0.31281705409126304
>1k	0.0030297811662784255	2.6806976984817847
>5k	4.32825880896918E-4	0.9670887483761218
>10k+	4.32825880896918E-4	2.526950186073981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17969	1.4851321485776816	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	11944	0.9871678102627764	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	6093	0.5035845167390403	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	5355	0.4425890509006336	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	4592	0.3795273429945302	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	3760	0.310762806981584	No Hit
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	3187	0.2634045387899756	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3054	0.2524121309898291	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	2829	0.2338159523805588	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	2611	0.2157983215502436	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	2026	0.16744825716614073	No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1726	0.14265335235378032	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1594	0.13174359423634172	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	1544	0.12761111010094833	Illumina PCR Primer Index 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	1336	0.11041997609771176	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	1213	0.10025406512464399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6529936541573618E-4	0.0	0.0	0.14306660076731964	0.0
2	1.6529936541573618E-4	0.0	0.0	0.6320221236670672	0.0
3	1.6529936541573618E-4	0.0	0.0	1.0887442703107464	0.0
4	1.6529936541573618E-4	0.0	0.0	1.9287956453535176	0.0
5	1.6529936541573618E-4	0.0	0.0	3.9550352666196114	0.0
6	1.6529936541573618E-4	0.0	0.0	5.7043158011316395	0.0
7	2.4794904812360424E-4	0.0	0.0	7.072498648677688	0.0
8	2.4794904812360424E-4	0.0	0.0	8.988566242894194	0.0
9	2.4794904812360424E-4	0.0	0.0	10.016232397683826	0.0
10	3.3059873083147236E-4	0.0	0.0	11.836013111545665	0.0
11	3.3059873083147236E-4	0.0	0.0	12.968479064008873	0.0
12	3.3059873083147236E-4	0.0	0.0	14.534525251957557	0.0
13	3.3059873083147236E-4	0.0	0.0	15.243576879908359	0.0
14	3.3059873083147236E-4	0.0	0.0	15.672032835065947	0.0
15	3.3059873083147236E-4	0.0	0.0	16.081314063835308	0.0
16	3.3059873083147236E-4	0.0	0.0	16.578286605957718	0.0
17	3.3059873083147236E-4	0.0	0.0	17.1159227919724	0.0
18	3.3059873083147236E-4	0.0	0.0	17.718191029864638	0.0
19	3.3059873083147236E-4	0.0	0.0	18.24334711379043	0.0
20	3.3059873083147236E-4	0.0	0.0	18.67643145117966	0.0
21	3.3059873083147236E-4	0.0	0.0	19.133897444967708	0.0
22	4.132484135393404E-4	0.0	0.0	19.667814395260535	0.0
23	4.132484135393404E-4	0.0	0.0	20.1968550142736	0.0
24	4.132484135393404E-4	0.0	0.0	20.621922332440167	0.0
25	4.132484135393404E-4	0.0	0.0	21.061535994763315	0.0
26	4.132484135393404E-4	0.0	0.0	21.465114395425836	0.0
27	4.132484135393404E-4	0.0	0.0	21.90456275838357	0.0
28	4.132484135393404E-4	0.0	0.0	22.353763783900835	0.0
29	4.132484135393404E-4	0.0	0.0	22.822718083585276	0.0
30	4.132484135393404E-4	0.0	0.0	23.373743518198633	0.0
31	4.958980962472085E-4	0.0	0.0	23.856830913626123	0.0
32	4.958980962472085E-4	0.0	0.0	24.344050793189005	0.0
33	4.958980962472085E-4	0.0	0.0	24.808789959055346	0.0
34	5.785477789550766E-4	0.0	0.0	25.271462882853992	0.0
35	5.785477789550766E-4	0.0	0.0	25.742566074288842	0.0
36	5.785477789550766E-4	0.0	0.0	26.187717265353417	0.0
37	5.785477789550766E-4	0.0	0.0	26.665597730770312	0.0
38	6.611974616629447E-4	0.0	0.0	27.145957686668442	0.0
39	8.264968270786808E-4	0.0	0.0	27.739382408510934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	40	6.8157533E-9	45.0	1
CCGACTT	25	3.8909282E-5	45.0	44
CGTTATT	880	0.0	44.74432	1
CGTTTTT	12285	0.0	43.9011	1
CGTAAGG	145	0.0	41.89655	2
CGTTTCT	505	0.0	41.88119	1
CTATGCG	45	1.9284926E-8	40.0	1
TCAACGG	45	1.9284926E-8	40.0	2
CATACGG	75	0.0	39.0	2
TTAACGG	105	0.0	38.57143	2
ACTTGCG	35	6.2485615E-6	38.571426	1
TACGGCT	915	0.0	38.11475	7
CGCTAGG	130	0.0	38.07692	2
GTTTTTT	14255	0.0	37.565765	2
GCGAGAC	240	0.0	37.499996	21
GTAACCG	30	1.13995746E-4	37.499996	1
CGTTCTG	355	0.0	37.394367	1
CGCATGG	170	0.0	37.058823	2
CAAGGGA	1230	0.0	36.585365	4
GACACGA	240	0.0	36.5625	25
>>END_MODULE