##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935864.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312476
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10177101601403	33.0	31.0	34.0	30.0	34.0
2	32.210137738578325	33.0	31.0	34.0	30.0	34.0
3	32.15472548291709	33.0	31.0	34.0	30.0	34.0
4	35.77597639498713	37.0	35.0	37.0	35.0	37.0
5	32.243442696399086	37.0	35.0	37.0	0.0	37.0
6	33.97010330393374	37.0	35.0	37.0	18.0	37.0
7	35.586297827673164	37.0	35.0	37.0	32.0	37.0
8	35.81234398801828	37.0	35.0	37.0	35.0	37.0
9	37.754054711401835	39.0	38.0	39.0	35.0	39.0
10	37.394523099374034	39.0	37.0	39.0	34.0	39.0
11	37.403563793699355	39.0	37.0	39.0	35.0	39.0
12	37.313617685838274	39.0	37.0	39.0	35.0	39.0
13	37.22190184206147	39.0	37.0	39.0	34.0	39.0
14	38.4365679284169	40.0	38.0	41.0	34.0	41.0
15	38.59328396420845	40.0	38.0	41.0	35.0	41.0
16	38.52769492697039	40.0	38.0	41.0	34.0	41.0
17	38.532344884087095	40.0	38.0	41.0	34.0	41.0
18	38.45397406520821	40.0	38.0	41.0	34.0	41.0
19	38.34835955401375	40.0	37.0	41.0	34.0	41.0
20	38.464336461040205	40.0	38.0	41.0	34.0	41.0
21	38.37711376233695	40.0	37.0	41.0	34.0	41.0
22	38.45247955042947	40.0	38.0	41.0	34.0	41.0
23	38.466086995481255	40.0	38.0	41.0	34.0	41.0
24	38.437297584454484	40.0	37.0	41.0	34.0	41.0
25	38.15084038454153	40.0	37.0	41.0	34.0	41.0
26	38.21240991308132	40.0	37.0	41.0	34.0	41.0
27	38.18279803888939	40.0	37.0	41.0	34.0	41.0
28	38.113688731294566	40.0	37.0	41.0	34.0	41.0
29	38.088537999718376	40.0	37.0	41.0	34.0	41.0
30	37.96372521409644	40.0	37.0	41.0	34.0	41.0
31	37.92568389252295	40.0	37.0	41.0	34.0	41.0
32	37.8886506483698	40.0	37.0	41.0	34.0	41.0
33	37.85167820888644	40.0	37.0	41.0	34.0	41.0
34	37.78446984728427	40.0	36.0	41.0	33.0	41.0
35	37.80564907384887	40.0	37.0	41.0	33.0	41.0
36	37.710156940053	40.0	36.0	41.0	33.0	41.0
37	37.64868021864079	40.0	36.0	41.0	33.0	41.0
38	37.532364085561774	40.0	36.0	41.0	33.0	41.0
39	37.465664563038445	40.0	36.0	41.0	33.0	41.0
40	37.40588397188904	40.0	36.0	41.0	33.0	41.0
41	37.29275208336	40.0	35.0	41.0	33.0	41.0
42	37.29453142001306	40.0	35.0	41.0	33.0	41.0
43	37.23490764090682	40.0	35.0	41.0	33.0	41.0
44	37.17978980785724	39.0	35.0	41.0	33.0	41.0
45	37.13226935828671	39.0	35.0	41.0	33.0	41.0
46	37.027560516647675	39.0	35.0	41.0	32.0	41.0
47	36.95648625814462	39.0	35.0	41.0	32.0	41.0
48	36.92834969725675	39.0	35.0	41.0	32.0	41.0
49	36.898757024539485	39.0	35.0	41.0	32.0	41.0
50	36.764980350490916	39.0	35.0	41.0	32.0	41.0
51	35.77284335436961	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	7.0
10	12.0
11	8.0
12	7.0
13	6.0
14	6.0
15	9.0
16	15.0
17	32.0
18	60.0
19	101.0
20	176.0
21	332.0
22	493.0
23	684.0
24	1038.0
25	1378.0
26	1819.0
27	2217.0
28	2554.0
29	3048.0
30	3840.0
31	4982.0
32	6677.0
33	10181.0
34	17657.0
35	24173.0
36	22901.0
37	36667.0
38	61854.0
39	109457.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.315646641662084	21.14242373814309	24.356430573868074	11.185499046326758
2	33.12318386052049	24.04824690536233	24.055927495231632	18.772641738885547
3	32.36952597959523	24.913593363970353	25.373788706972693	17.34309194946172
4	29.293769761517684	25.725495718071144	24.943355649713897	20.03737887069727
5	23.181940373020648	36.462320306199516	21.959766510067976	18.395972810711864
6	25.462435515047556	35.14797936481522	24.2217002265774	15.167884893559824
7	81.1127894622307	5.515303575314584	8.8109166784009	4.5609902840538155
8	81.38289020596781	5.585068933294076	7.93628950703414	5.095751353703965
9	75.11040847937122	7.998694299722218	10.101575801021518	6.789321419885047
10	37.231339366863374	32.09782511296868	16.790729528027754	13.880105992140196
11	32.32760275989196	25.864066360296473	23.09009331916691	18.718237560644656
12	30.513383427847256	23.008167027227692	27.201769095866563	19.276680449058485
13	25.763578642839768	25.10784828274811	29.163199733739553	19.965373340672564
14	20.285397918560143	29.029109435604656	28.901419629027508	21.78407301680769
15	19.732075423392516	26.549879030709562	33.71682945250195	20.001216093395975
16	22.23946799114172	24.776942869212355	32.53657880925255	20.447010330393375
17	22.043292924896633	24.19673830950217	28.79165119881207	24.968317566789132
18	23.646616060113416	24.41915539113404	29.145598381955732	22.78863016679681
19	24.471639421907604	26.899665894340686	26.544438612885468	22.084256070866243
20	27.46546934804593	25.86790665523112	26.955350170893123	19.71127382582982
21	25.74917753683483	25.915270292758485	27.64276296419565	20.69278920621104
22	22.605256083667225	25.648689819378127	26.8167795286678	24.929274568286843
23	22.371318117231404	27.782293680154634	27.63732254637156	22.209065656242398
24	23.080172557252396	24.951676288738973	28.553552912863704	23.414598241144922
25	22.284271432045983	27.346420205071748	26.345063300861504	24.024245062020764
26	21.949525723575572	28.348417158437766	26.87502400184334	22.82703311614332
27	24.08408965808574	27.983269115068037	26.92942818008423	21.003213046761992
28	20.89344461654655	27.76053200885828	28.91422061214301	22.431802762452158
29	21.549495001216094	27.92182439611362	28.726366184923002	21.802314417747283
30	24.346509811953556	25.38370946888721	27.154725482917087	23.115055236242142
31	23.42131875728056	26.81453935662259	26.387626569720556	23.3765153163763
32	24.24954236485362	27.39890423584531	26.80685876675329	21.544694632547778
33	23.309630179597793	26.396587257901405	26.845261716099795	23.448520846401003
34	21.742469821682306	25.91175002240172	29.57059102139044	22.77518913452553
35	22.235627696207068	25.411871631741317	27.62292144036662	24.72957923168499
36	22.633098221943445	27.22929120956489	27.474110011648893	22.663500556842767
37	22.95440289814258	26.804938619285963	29.076473073132014	21.164185409439444
38	21.37284143422215	26.494514778735006	27.96662783701788	24.166015950024963
39	22.68654232645067	23.51252576197852	28.917100833344	24.88383107822681
40	24.612450236178137	23.246585337753938	28.883178228087917	23.257786197980003
41	20.22139300298263	26.03911980440098	28.669721834636903	25.069765357979495
42	22.381878928301692	25.973834790511912	28.396740869698796	23.247545411487604
43	23.162418873769504	24.88191093075948	29.054071352679884	22.901598842791127
44	22.814872182183592	25.35170701109845	27.758611861390953	24.074808945327
45	22.28139121084499	24.094650469156033	27.91062353588756	25.713334784111417
46	22.395639985150858	25.641009229508825	27.092640714806898	24.870710070533416
47	21.210908997811032	25.97255469220036	29.643556625148808	23.172979684839795
48	21.445807037980515	24.152574917753682	29.59107259437525	24.81054544989055
49	21.915923142897377	24.147454524507484	30.035906757638987	23.90071557495616
50	20.206031823244025	24.960957001497714	29.889015476388586	24.943995698869674
51	20.718711197019932	23.56564984190786	27.337779541468787	28.377859419603425
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	332.0
2	541.0
3	794.0
4	1047.0
5	767.0
6	487.0
7	498.5
8	510.0
9	519.5
10	529.0
11	533.0
12	537.0
13	520.5
14	504.0
15	574.0
16	644.0
17	617.0
18	590.0
19	651.5
20	713.0
21	856.0
22	999.0
23	1196.0
24	1393.0
25	1527.0
26	2023.5
27	2386.0
28	3106.5
29	3827.0
30	4147.5
31	4468.0
32	5220.0
33	5972.0
34	6873.5
35	7775.0
36	8834.0
37	9893.0
38	10752.5
39	11612.0
40	12806.5
41	14001.0
42	15545.0
43	17089.0
44	19204.5
45	21320.0
46	24068.0
47	26816.0
48	28548.5
49	30281.0
50	30725.5
51	31170.0
52	27353.5
53	23537.0
54	21396.0
55	19255.0
56	17308.5
57	15362.0
58	14712.5
59	14063.0
60	13815.0
61	13567.0
62	12309.5
63	11052.0
64	9163.0
65	7274.0
66	5761.0
67	4248.0
68	3540.5
69	2833.0
70	2303.5
71	1774.0
72	1519.0
73	1264.0
74	907.5
75	463.5
76	376.0
77	315.0
78	254.0
79	187.0
80	120.0
81	71.0
82	22.0
83	17.0
84	12.0
85	9.0
86	6.0
87	6.5
88	7.0
89	6.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	312476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.307420175267936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00141322374354	22.982590629305154
2	11.58580218079854	7.717863626067851
3	4.336355851090785	4.332984790852871
4	2.3393531755402353	3.1167127662426375
5	1.5264814386467578	2.542157933337752
6	1.180227437391789	2.3586198695752825
7	0.9577442277850543	2.2329992590692025
8	0.7761712277616544	2.0681808968808806
9	0.669422664199778	2.006706775621437
>10	7.519282453266195	46.67362616726379
>50	0.08280488834611524	1.8525236070553501
>100	0.02194828365800645	1.371698197295872
>500	0.0029929477715463343	0.7433354814318957
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	916	0.2931425133450249	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	813	0.26017998182260393	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	508	0.16257248556689152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.14369103547152423	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.200245778875818E-4	0.0	0.0	0.16993305085830593	0.0
2	3.200245778875818E-4	0.0	0.0	0.9171904402258093	0.0
3	3.200245778875818E-4	0.0	0.0	1.2471357800279061	0.0
4	3.200245778875818E-4	0.0	0.0	1.6295651506035664	0.0
5	3.200245778875818E-4	0.0	0.0	2.7893342208681626	0.0
6	3.200245778875818E-4	0.0	0.0	3.500748857512257	0.0
7	3.200245778875818E-4	0.0	0.0	4.139197890397982	0.0
8	3.200245778875818E-4	0.0	0.0	5.244242757843803	0.0
9	3.200245778875818E-4	0.0	0.0	5.6583545616303335	0.0
10	3.200245778875818E-4	0.0	0.0	6.699074488920749	0.0
11	3.200245778875818E-4	0.0	0.0	8.060459043254522	0.0
12	3.200245778875818E-4	0.0	0.0	9.20422688462474	0.0
13	3.200245778875818E-4	0.0	0.0	9.677863259898361	0.0
14	3.200245778875818E-4	0.0	0.0	9.84203586835469	0.0
15	3.200245778875818E-4	0.0	0.0	10.229585632176551	0.0
16	3.200245778875818E-4	0.0	0.0	10.975562923232504	0.0
17	3.200245778875818E-4	0.0	0.0	11.792265646001614	0.0
18	3.200245778875818E-4	0.0	0.0	12.712976356584186	0.0
19	3.200245778875818E-4	0.0	0.0	13.24453718045546	0.0
20	3.200245778875818E-4	0.0	0.0	13.76457711952278	0.0
21	3.200245778875818E-4	0.0	0.0	14.420307479614435	0.0
22	3.200245778875818E-4	0.0	0.0	15.119881206876688	0.0
23	3.200245778875818E-4	0.0	0.0	15.788412550083846	0.0
24	3.200245778875818E-4	0.0	0.0	16.32765396382442	0.0
25	3.200245778875818E-4	0.0	0.0	16.783688987314225	0.0
26	3.200245778875818E-4	0.0	0.0	17.22788310142219	0.0
27	3.200245778875818E-4	0.0	0.0	17.683278075756217	0.0
28	3.200245778875818E-4	0.0	0.0	18.149233861160535	0.0
29	3.200245778875818E-4	0.0	0.0	18.648152178087276	0.0
30	3.200245778875818E-4	0.0	0.0	19.207235115656882	0.0
31	6.400491557751636E-4	0.0	0.0	19.712553924141375	0.0
32	6.400491557751636E-4	0.0	0.0	20.185870274837107	0.0
33	6.400491557751636E-4	0.0	0.0	20.641585273749023	0.0
34	6.400491557751636E-4	0.0	0.0	21.11202140324377	0.0
35	6.400491557751636E-4	0.0	0.0	21.635261588089964	0.0
36	6.400491557751636E-4	0.0	0.0	22.138980273685018	0.0
37	6.400491557751636E-4	0.0	0.0	22.666380778043752	0.0
38	6.400491557751636E-4	0.0	0.0	23.13905707958371	0.0
39	9.600737336627453E-4	0.0	0.0	23.642455740600877	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAATC	25	3.8842656E-5	45.0	12
CGCGCAT	45	3.8198777E-10	45.0	36
TACGGGT	20	7.0253934E-4	45.0	4
TACGCGG	25	3.8842656E-5	45.0	2
ATGAGTC	20	7.0253934E-4	45.0	18
TTGCGAG	25	3.8842656E-5	45.0	1
CGAATCT	20	7.0253934E-4	45.0	25
TTAACGG	40	6.7884685E-9	45.0	2
CGGTCTA	35	1.2081364E-7	45.0	31
CGCACTG	20	7.0253934E-4	45.0	31
GCTACGA	40	6.7884685E-9	45.0	10
TATACGG	20	7.0253934E-4	45.0	2
CTCACGA	30	2.16041E-6	44.999996	24
CGACGGT	30	2.16041E-6	44.999996	28
ACGGGTA	30	2.16041E-6	44.999996	5
ACTACGG	30	2.16041E-6	44.999996	2
AACGAGC	80	0.0	42.1875	15
CATTGGG	105	0.0	40.714287	3
CGGGTAT	50	1.0768417E-9	40.5	6
CGCATGT	50	1.0768417E-9	40.5	38
>>END_MODULE