##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181535
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09850992921475	33.0	31.0	34.0	30.0	34.0
2	32.19957033079021	33.0	31.0	34.0	30.0	34.0
3	32.15748478254882	33.0	31.0	34.0	30.0	34.0
4	35.79400115680172	37.0	35.0	37.0	35.0	37.0
5	31.906673644200843	37.0	35.0	37.0	0.0	37.0
6	33.77811441319856	37.0	35.0	37.0	17.0	37.0
7	35.46943013743906	37.0	35.0	37.0	32.0	37.0
8	35.74441843170739	37.0	35.0	37.0	35.0	37.0
9	37.70189219709698	39.0	37.0	39.0	35.0	39.0
10	37.31880353650811	39.0	37.0	39.0	34.0	39.0
11	37.2797752499518	39.0	37.0	39.0	34.0	39.0
12	37.106249483570664	39.0	37.0	39.0	34.0	39.0
13	36.97877544275209	39.0	37.0	39.0	33.0	39.0
14	38.13922384113256	40.0	37.0	41.0	33.0	41.0
15	38.310794061751174	40.0	37.0	41.0	34.0	41.0
16	38.307742308645715	40.0	37.0	41.0	34.0	41.0
17	38.2923513372077	40.0	37.0	41.0	34.0	41.0
18	38.270680585011156	40.0	37.0	41.0	34.0	41.0
19	38.129556283912194	40.0	37.0	41.0	34.0	41.0
20	38.16853499325199	40.0	37.0	41.0	34.0	41.0
21	38.119299308673256	40.0	37.0	41.0	34.0	41.0
22	38.18602473352246	40.0	37.0	41.0	34.0	41.0
23	38.176786845511884	40.0	37.0	41.0	34.0	41.0
24	38.18795824496654	40.0	36.0	41.0	34.0	41.0
25	37.93183683587187	40.0	36.0	41.0	34.0	41.0
26	37.94881427823836	40.0	36.0	41.0	34.0	41.0
27	37.905417688049134	40.0	36.0	41.0	34.0	41.0
28	37.84677334949183	40.0	36.0	41.0	34.0	41.0
29	37.81000908915636	40.0	36.0	41.0	33.0	41.0
30	37.66381138623406	40.0	36.0	41.0	33.0	41.0
31	37.674117938689506	40.0	36.0	41.0	33.0	41.0
32	37.58475776021153	40.0	35.0	41.0	33.0	41.0
33	37.48765802737764	40.0	35.0	41.0	33.0	41.0
34	37.40768446855978	40.0	35.0	41.0	33.0	41.0
35	37.37576775828353	40.0	35.0	41.0	33.0	41.0
36	37.28838515988652	40.0	35.0	41.0	33.0	41.0
37	37.21580411490897	40.0	35.0	41.0	33.0	41.0
38	37.0744594706255	39.0	35.0	41.0	32.0	41.0
39	36.99713003002176	39.0	35.0	41.0	32.0	41.0
40	36.915520423058915	39.0	35.0	41.0	32.0	41.0
41	36.850480623571215	39.0	35.0	41.0	31.0	41.0
42	36.857261685074505	39.0	35.0	41.0	32.0	41.0
43	36.783369598149115	39.0	35.0	41.0	32.0	41.0
44	36.73869501748974	39.0	35.0	41.0	32.0	41.0
45	36.713575894455616	39.0	35.0	41.0	32.0	41.0
46	36.58217974495276	39.0	35.0	41.0	31.0	41.0
47	36.464224529705014	39.0	35.0	41.0	31.0	41.0
48	36.42643567356157	38.0	35.0	41.0	31.0	41.0
49	36.392332057179054	38.0	35.0	41.0	31.0	41.0
50	36.29923155314402	38.0	35.0	40.0	31.0	41.0
51	35.29625692015314	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	6.0
11	7.0
12	4.0
13	4.0
14	3.0
15	8.0
16	10.0
17	19.0
18	40.0
19	84.0
20	159.0
21	261.0
22	454.0
23	638.0
24	761.0
25	1044.0
26	1335.0
27	1414.0
28	1549.0
29	1900.0
30	2416.0
31	3217.0
32	4304.0
33	6656.0
34	11782.0
35	16310.0
36	14133.0
37	21316.0
38	34833.0
39	56806.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.68281598589804	19.202908530035533	22.508056297683645	10.606219186382791
2	32.10895970474013	25.302558735230118	24.331947007464123	18.256534552565622
3	31.440218139752663	26.019224942848485	25.331203349216402	17.209353568182443
4	28.55647671247969	25.249125512986474	27.213485002892	18.98091277164183
5	23.861514308535543	37.421433883273195	20.93480596028314	17.78224584790812
6	24.98251025972953	35.90657448976781	23.99977965681549	15.111135593687166
7	79.13460214283748	5.958630567108271	10.458038394799901	4.448728895254359
8	79.6606714958548	7.280689674167516	8.136172088027102	4.922466741950588
9	74.42421571597764	6.911063982152202	9.923706172363456	8.741014129506706
10	41.848128460076566	24.697716693750515	18.231745944308262	15.222408901864654
11	33.68110832621809	25.090478420139366	22.85013909163522	18.378274162007326
12	28.211088770760462	22.929462638058776	28.062357121216298	20.79709146996447
13	25.49425730575371	26.699534525022724	29.705015561737408	18.101192607486162
14	21.54736001322059	30.004131434709556	28.341642107582558	20.106866444487288
15	19.89313355551271	24.411821411848955	34.62417715592034	21.070867876717987
16	22.768061255405296	24.07249290770375	30.661304982510263	22.498140854380697
17	22.71738232296802	24.37215963863718	28.24909797008841	24.661360068306386
18	24.685597818602474	23.32608037017655	29.46539234858292	22.522929462638057
19	23.901176081747323	25.9514694136117	26.80309582174236	23.344258682898612
20	26.273170462996116	26.083675324317625	27.287850827664084	20.35530338502217
21	24.261437188420963	27.7902332883466	28.422067369928666	19.52626215330377
22	23.394937615335888	23.35417412620156	28.655080287547857	24.5958079709147
23	22.346104057068885	26.947971465557607	27.945575233426062	22.76034924394745
24	22.623736469551325	25.115817886358005	28.69804720852728	23.562398435563388
25	21.535241138072546	28.248547112127138	27.52306717712838	22.693144572671937
26	21.037816399041507	27.45365907400777	28.05739939956482	23.451125127385904
27	22.747128652876857	27.501032858677387	26.927038863029168	22.824799625416585
28	20.062797807585316	26.690169939681052	29.66865893629328	23.578373316440356
29	21.70490539014515	26.528217699066296	28.95309444459746	22.813782466191093
30	22.74988294268323	28.368083289723746	27.941168369735863	20.940865397857163
31	25.459553254193406	27.25535020794888	24.30164981959402	22.983446718263696
32	26.014818079158292	27.95383810284518	24.921915883989314	21.109427934007215
33	24.382625939901395	28.050789104029526	24.341862450767067	23.224722505302008
34	22.741069215302833	27.333021180488608	26.616905830831524	23.309003773377036
35	22.929462638058776	28.576307599085577	25.49040130002479	23.003828462830857
36	24.948907924091774	30.147354504640976	24.169443908888095	20.734293662379155
37	23.933676701462527	27.784173850772582	26.503429090808933	21.778720356955958
38	23.578373316440356	29.638912606384444	24.290632660368523	22.492081416806677
39	23.814691381827195	26.862588481560028	25.381331423692398	23.94138871292037
40	24.49445010604016	26.214779519101	26.490759357699616	22.800011017159225
41	21.52257140496323	27.30547828242488	25.092130994023194	26.079819318588704
42	24.276861211336655	26.10681135869116	26.829537003883548	22.786790426088633
43	24.13418899936651	26.814663838929132	26.00214834604897	23.048998815655384
44	22.78789214201118	26.169609166276476	26.913818271958572	24.128680419753767
45	23.128873220040212	25.41438290136888	25.5576059713003	25.899137907290605
46	24.645936045390695	27.41895502244746	25.483240146528217	22.451868785633625
47	21.938469165725618	27.026744154019887	28.391219324097282	22.643567356157217
48	23.09912689013138	27.236070179304267	26.5194039716859	23.145398958878452
49	22.831409920951884	25.20009915443303	29.09080893491613	22.877681989698956
50	21.98914809816289	26.01096207342937	28.25130140193351	23.748588426474235
51	22.082793951579585	25.676040432974357	25.624810642575813	26.616354972870244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	146.5
2	226.0
3	423.5
4	621.0
5	436.0
6	251.0
7	267.5
8	284.0
9	267.5
10	251.0
11	272.5
12	294.0
13	278.5
14	263.0
15	277.0
16	291.0
17	283.5
18	276.0
19	304.0
20	332.0
21	371.0
22	410.0
23	494.0
24	578.0
25	648.0
26	974.5
27	1231.0
28	1476.0
29	1721.0
30	1933.5
31	2146.0
32	2611.0
33	3076.0
34	3715.0
35	4354.0
36	4710.5
37	5067.0
38	5912.5
39	6758.0
40	7480.0
41	8202.0
42	9481.0
43	10760.0
44	12222.5
45	13685.0
46	15995.5
47	18306.0
48	17741.5
49	17177.0
50	17539.0
51	17901.0
52	16470.0
53	15039.0
54	13208.5
55	11378.0
56	10142.5
57	8907.0
58	8415.0
59	7923.0
60	7023.5
61	6124.0
62	5579.0
63	5034.0
64	4534.0
65	4034.0
66	3475.5
67	2917.0
68	2389.5
69	1862.0
70	1522.0
71	1182.0
72	993.0
73	804.0
74	690.5
75	436.5
76	296.0
77	208.0
78	120.0
79	90.5
80	61.0
81	43.5
82	26.0
83	15.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	181535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.49486324951111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97934362665104	28.823091965736637
2	9.443909787020377	7.459718511581788
3	3.742137048969971	4.433855730299943
4	2.106085331324881	3.3271820860990995
5	1.56352427577165	3.0875588729446113
6	1.2329665118484734	2.921750626600931
7	1.0711745261308006	2.9614123998127084
8	0.9024087479252967	2.8512408075577715
9	0.8549869590080478	3.039083372352439
>10	6.054646637934642	37.222574159253035
>50	0.022316135961058345	0.5800534332222437
>100	0.020921377463492197	1.3832043407607348
>500	0.005579033990264586	1.9092736937780592
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTC	950	0.523315063210951	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCG	935	0.5150521937918308	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGC	911	0.5018316027212384	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	670	0.3690748340540392	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGC	381	0.20987688324565512	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	0.1531385132343625	No Hit
CTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTTCTGCT	263	0.14487564381524223	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCTT	201	0.11072245021621174	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGACGATCGTATGCCGTCT	192	0.10576472856473958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15203679731181316	0.0
2	0.0	0.0	0.0	0.9166276475610764	0.0
3	0.0	0.0	0.0	1.1893023383920456	0.0
4	0.0	0.0	0.0	1.590877792161291	0.0
5	0.0	0.0	0.0	3.060566832842152	0.0
6	0.0	0.0	0.0	3.740876415016388	0.0
7	0.0	0.0	0.0	4.438813451951415	0.0
8	0.0	0.0	0.0	5.381331423692401	0.0
9	5.508579612746854E-4	0.0	0.0	5.717354780069959	0.0
10	5.508579612746854E-4	0.0	0.0	7.0642024953865645	0.0
11	5.508579612746854E-4	0.0	0.0	8.224309361831052	0.0
12	5.508579612746854E-4	0.0	0.0	9.724846448343294	0.0
13	5.508579612746854E-4	0.0	0.0	10.167185391246868	0.0
14	5.508579612746854E-4	0.0	0.0	10.375409700608698	0.0
15	5.508579612746854E-4	0.0	0.0	10.75770512573333	0.0
16	5.508579612746854E-4	0.0	0.0	11.478778197041892	0.0
17	5.508579612746854E-4	0.0	0.0	12.229046740298013	0.0
18	5.508579612746854E-4	0.0	0.0	13.07516456881593	0.0
19	5.508579612746854E-4	0.0	0.0	13.62987853581954	0.0
20	5.508579612746854E-4	0.0	0.0	14.097556944941747	0.0
21	5.508579612746854E-4	0.0	0.0	14.705153276227724	0.0
22	5.508579612746854E-4	0.0	0.0	15.292918720907814	0.0
23	5.508579612746854E-4	0.0	0.0	15.885091029278101	0.0
24	5.508579612746854E-4	0.0	0.0	16.384168342192964	0.0
25	5.508579612746854E-4	0.0	0.0	16.790150659652408	0.0
26	5.508579612746854E-4	0.0	0.0	17.209353568182443	0.0
27	5.508579612746854E-4	0.0	0.0	17.61423416971934	0.0
28	5.508579612746854E-4	0.0	0.0	18.02737764067535	0.0
29	5.508579612746854E-4	0.0	0.0	18.43280910017352	0.0
30	5.508579612746854E-4	0.0	0.0	18.931886413088385	0.0
31	5.508579612746854E-4	0.0	0.0	19.377530503759605	0.0
32	5.508579612746854E-4	0.0	0.0	19.79673341228964	0.0
33	5.508579612746854E-4	0.0	0.0	20.24127578703831	0.0
34	5.508579612746854E-4	0.0	0.0	20.661029553529623	0.0
35	5.508579612746854E-4	0.0	0.0	21.179937753050375	0.0
36	5.508579612746854E-4	0.0	0.0	21.58702178643237	0.0
37	5.508579612746854E-4	0.0	0.0	22.007326410884954	0.0
38	5.508579612746854E-4	0.0	0.0	22.451868785633625	0.0
39	5.508579612746854E-4	0.0	0.0	22.990057013798992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTATGG	55	1.8189894E-12	45.000004	2
CGTTAGG	25	3.8777907E-5	45.000004	2
TGCGAAG	25	3.8777907E-5	45.000004	1
CGGGAAC	25	3.8777907E-5	45.000004	6
CGGTAGG	25	3.8777907E-5	45.000004	31
GCGAGAC	45	3.8016879E-10	45.0	21
GTTTGCG	20	7.017579E-4	45.0	1
AGGTACA	20	7.017579E-4	45.0	36
ACCGGCG	20	7.017579E-4	45.0	20
CTCCGAC	20	7.017579E-4	45.0	21
TACGGGT	20	7.017579E-4	45.0	4
AAGCCCG	20	7.017579E-4	45.0	14
GTGTACG	20	7.017579E-4	45.0	1
AAACACG	45	3.8016879E-10	45.0	40
CGTAAGG	35	1.2043893E-7	45.0	2
AAGGGCG	70	0.0	45.0	5
CGAGACA	45	3.8016879E-10	45.0	22
CGATGAG	20	7.017579E-4	45.0	10
CCACGAG	20	7.017579E-4	45.0	35
CGTCATA	20	7.017579E-4	45.0	38
>>END_MODULE