##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506717
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942518210361996	33.0	31.0	34.0	30.0	34.0
2	32.087316983641756	33.0	31.0	34.0	30.0	34.0
3	31.94261096430552	33.0	31.0	34.0	30.0	34.0
4	35.66117971175232	37.0	35.0	37.0	33.0	37.0
5	31.953836164959927	37.0	35.0	37.0	0.0	37.0
6	33.800762555825045	37.0	35.0	37.0	17.0	37.0
7	35.498007763702425	37.0	35.0	37.0	32.0	37.0
8	35.76555158007329	37.0	35.0	37.0	35.0	37.0
9	37.73368171977652	39.0	37.0	39.0	35.0	39.0
10	37.30643337405297	39.0	37.0	39.0	34.0	39.0
11	37.28864829875453	39.0	37.0	39.0	34.0	39.0
12	37.370950648981584	39.0	37.0	39.0	34.0	39.0
13	37.39319975449807	39.0	37.0	39.0	34.0	39.0
14	38.66344527616007	40.0	38.0	41.0	35.0	41.0
15	38.768543388123945	40.0	38.0	41.0	35.0	41.0
16	38.6880092832883	40.0	38.0	41.0	35.0	41.0
17	38.6782563048013	40.0	38.0	41.0	35.0	41.0
18	38.5143186433453	40.0	38.0	41.0	35.0	41.0
19	38.282702178928275	40.0	37.0	41.0	34.0	41.0
20	38.24758790409637	40.0	37.0	41.0	34.0	41.0
21	38.19155465476785	40.0	37.0	41.0	34.0	41.0
22	38.27335179202592	40.0	37.0	41.0	34.0	41.0
23	38.264990122691756	40.0	37.0	41.0	34.0	41.0
24	38.228277322450204	40.0	37.0	41.0	34.0	41.0
25	37.97713319268941	40.0	36.0	41.0	34.0	41.0
26	38.019273085371125	40.0	36.0	41.0	34.0	41.0
27	37.96608165899309	40.0	36.0	41.0	34.0	41.0
28	37.88306885302842	40.0	36.0	41.0	34.0	41.0
29	37.82823351101305	40.0	36.0	41.0	33.0	41.0
30	37.62738175352317	40.0	36.0	41.0	33.0	41.0
31	37.522427706194975	40.0	36.0	41.0	33.0	41.0
32	37.26192529557919	40.0	36.0	41.0	32.0	41.0
33	36.97143967934765	40.0	36.0	41.0	31.0	41.0
34	36.66019494116045	40.0	36.0	41.0	30.0	41.0
35	36.54544844558205	40.0	36.0	41.0	30.0	41.0
36	36.41086050004243	40.0	36.0	41.0	29.0	41.0
37	36.34143516005976	40.0	36.0	41.0	29.0	41.0
38	36.219538716877466	40.0	35.0	41.0	27.0	41.0
39	36.12242731149735	40.0	35.0	41.0	27.0	41.0
40	36.096783806345556	40.0	35.0	41.0	27.0	41.0
41	35.96233795195346	40.0	35.0	41.0	26.0	41.0
42	35.94321287819434	40.0	35.0	41.0	26.0	41.0
43	35.858362754752655	40.0	35.0	41.0	25.0	41.0
44	35.81143123281832	39.0	35.0	41.0	25.0	41.0
45	35.78655541456079	39.0	35.0	41.0	25.0	41.0
46	35.657173530787404	39.0	35.0	41.0	24.0	41.0
47	35.54617863620127	39.0	35.0	41.0	24.0	41.0
48	35.51037364051334	39.0	35.0	41.0	24.0	41.0
49	35.47644937904195	39.0	35.0	41.0	24.0	41.0
50	35.36159631510291	39.0	35.0	41.0	24.0	41.0
51	34.33499961516981	38.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	9.0
10	16.0
11	8.0
12	3.0
13	6.0
14	8.0
15	16.0
16	31.0
17	62.0
18	142.0
19	289.0
20	525.0
21	894.0
22	1464.0
23	2381.0
24	3798.0
25	6432.0
26	8526.0
27	9005.0
28	8200.0
29	7617.0
30	7700.0
31	8898.0
32	11163.0
33	15000.0
34	23156.0
35	31796.0
36	36022.0
37	56340.0
38	97563.0
39	169532.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56939080393987	19.191382961297922	23.221048435319915	17.01817779944229
2	38.4459175437177	22.913776328798914	23.263478430761158	15.376827696722234
3	28.320936538541236	23.818423301369403	32.59393310269835	15.26670705739101
4	24.751488503444723	23.448196922542564	34.24929497135482	17.551019602657895
5	21.585816145896032	34.98224847400028	27.56331443389505	15.868620946208633
6	22.177467896281357	33.28268047055852	30.128059646706152	14.411791986453979
7	74.77921601209353	4.119064487672606	16.44941851171364	4.652300988520219
8	75.6968880065204	4.8247838537092695	14.940094766901446	4.53823337286888
9	70.00771633870583	6.028611631344518	16.834643400556917	7.129028629392738
10	31.683957712095705	30.374548317897364	24.22515921115731	13.716334758849612
11	22.6059121758299	24.06629341427266	34.795161796426804	18.532632613470636
12	20.605979274427344	21.826779050239086	37.067436063917334	20.49980561141623
13	20.821878879137664	23.010279899825743	38.95448544256458	17.213355778472007
14	18.310220497832123	26.93673194307671	35.57883394478575	19.174213614305422
15	16.97259022294496	25.71553746963295	39.16742481503482	18.14444749238727
16	20.16865429815854	26.161348444990008	34.398490676255186	19.27150658059627
17	19.81145294118808	25.227296498834654	34.40421379191936	20.55703676805791
18	20.81595841465749	24.35521208090512	35.57784720070572	19.250982303731668
19	18.928316989562223	27.72474576538778	33.27912819187041	20.067809053179587
20	19.915850464855136	26.511642593400257	34.576499308292405	18.9960076334522
21	19.261244442163967	28.389416577695243	34.788254587866604	17.56108439227419
22	19.480301627930384	23.52970198355295	34.87508806690914	22.114908321607523
23	17.56246583398623	27.11888490025004	34.19581344221726	21.122835823546477
24	18.318706496920374	25.342745556198036	35.133812364692716	21.204735582188874
25	17.986765788398653	28.53288916693144	32.69773857991739	20.782606464752515
26	18.350282307481297	27.551276156118703	33.61600262079228	20.482438915607727
27	19.747709273618213	26.04136036485849	32.71767081033397	21.493259551189322
28	18.342585703657072	25.86986424374947	34.41743616259174	21.370113890001715
29	19.78579759510733	25.081455723806386	32.884035862226845	22.248710818859443
30	19.65771821351958	25.80533118091558	32.1755536127661	22.361396992798742
31	21.113165731562194	25.66738435852754	30.840291523671006	22.37915838623926
32	21.26670311041469	26.89272315710742	31.008630063723935	20.83194366875396
33	20.063467379227458	25.203614640913962	30.190027174931966	24.542890804926614
34	19.340973363830305	26.346461634403422	32.32553871293049	21.987026288835782
35	19.448725817369457	25.72639165451327	32.27679355537706	22.548088972740207
36	19.722053927537463	28.049384567717283	30.352247901688713	21.876313603056538
37	19.743762297298098	26.752605497743314	32.37862554443605	21.12500666052254
38	20.269302194321483	26.497038781015835	30.66978214664201	22.563876878020668
39	21.376823749745913	25.859207407685158	30.951004209450243	21.812964633118685
40	20.055770775403232	24.56696736047932	33.5281823976697	21.84907946644774
41	18.038076480560157	27.465232072340186	31.502594150186397	22.994097296913267
42	20.235752895600502	26.531180126184832	32.227653700191624	21.00541327802304
43	21.107837313530037	26.49013157245563	30.439278729547265	21.96275238446707
44	20.894503243427792	25.527464047979443	29.82710270229734	23.750930006295427
45	20.443363850038583	24.32482036324023	30.25238940078979	24.979426385931397
46	21.806649471006498	25.106519023439116	30.320277393495775	22.766554112058603
47	19.445173538681352	24.924563415081792	33.138615834874294	22.491647211362555
48	19.609170404782155	25.064483725629888	31.616267068205723	23.71007880138223
49	20.861151293522813	23.666859410676967	32.40211005354073	23.069879242259486
50	19.95078120528816	23.44464464385446	32.40171535590872	24.20285879494866
51	19.438068981305147	22.962324137536335	30.711422746819228	26.888184134339287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	265.5
2	405.0
3	5256.5
4	10108.0
5	7045.5
6	3983.0
7	3905.5
8	3828.0
9	3824.0
10	3820.0
11	3840.5
12	3861.0
13	3731.5
14	3602.0
15	3387.0
16	3172.0
17	3063.0
18	2954.0
19	2881.0
20	2808.0
21	2854.0
22	2900.0
23	2944.0
24	2988.0
25	3444.0
26	4359.5
27	4819.0
28	5608.5
29	6398.0
30	7739.0
31	9080.0
32	9624.5
33	10169.0
34	11966.0
35	13763.0
36	15613.5
37	17464.0
38	19084.5
39	20705.0
40	22609.0
41	24513.0
42	26930.0
43	29347.0
44	32903.0
45	36459.0
46	46227.5
47	55996.0
48	56650.0
49	57304.0
50	55405.0
51	53506.0
52	45938.0
53	38370.0
54	33073.5
55	27777.0
56	22858.5
57	17940.0
58	15169.5
59	12399.0
60	10349.0
61	8299.0
62	6876.5
63	5454.0
64	4493.5
65	3533.0
66	2776.5
67	2020.0
68	1608.0
69	1196.0
70	938.5
71	681.0
72	555.5
73	430.0
74	369.0
75	239.5
76	171.0
77	119.5
78	68.0
79	59.5
80	51.0
81	40.5
82	30.0
83	19.0
84	8.0
85	5.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	506717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.47302876053218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4135389154443	35.51168591711488
2	9.811900670486216	9.119774841099817
3	3.7214085239548886	5.188353760903355
4	2.0802740117398044	3.8670653590948643
5	1.3862488480755233	3.22115912929342
6	1.0572827478745779	2.948107892999385
7	0.8471631942481359	2.7559167643810554
8	0.6510162345179256	2.4203756952259936
9	0.5465790426278783	2.2861065211154576
>10	3.413618126025322	25.277445893199772
>50	0.04617304807838103	1.4337132258474006
>100	0.01881124180667976	1.7808164691847201
>500	0.002137641114395427	0.7120881652971144
>1k	0.003420225783032684	2.4118410263788146
>5k	4.275282228790855E-4	1.0655493388639576
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5363	1.0583817002389895	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	2276	0.4491659052291516	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	1944	0.38364609831523316	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	1672	0.32996722036166143	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1528	0.30154899085682935	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	1230	0.2427390436871074	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	1195	0.23583183512690514	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	1195	0.23583183512690514	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1099	0.21688634879035046	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	959	0.18925751454954146	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	801	0.15807640162062847	No Hit
CGTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	642	0.1266979398757097	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	616	0.12156687065955948	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC	566	0.11169942985927055	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9469763201155676E-4	0.0	0.0	0.26148718120765635	0.0
2	3.9469763201155676E-4	0.0	0.0	0.9958221255651577	0.0
3	3.9469763201155676E-4	0.0	0.0	1.5184017903484588	0.0
4	3.9469763201155676E-4	0.0	0.0	2.153864977887065	0.0
5	3.9469763201155676E-4	0.0	0.0	3.9807229676525555	0.0
6	3.9469763201155676E-4	0.0	0.0	5.435775788063159	0.0
7	3.9469763201155676E-4	0.0	0.0	6.671968771523355	0.0
8	3.9469763201155676E-4	0.0	0.0	8.403112585526044	0.0
9	3.9469763201155676E-4	0.0	0.0	9.18342980401289	0.0
10	3.9469763201155676E-4	0.0	0.0	10.926809244607936	0.0
11	3.9469763201155676E-4	0.0	0.0	12.611576086849267	0.0
12	3.9469763201155676E-4	0.0	0.0	14.346469528356065	0.0
13	3.9469763201155676E-4	0.0	0.0	15.06620066032914	0.0
14	3.9469763201155676E-4	0.0	0.0	15.385116346994476	0.0
15	3.9469763201155676E-4	0.0	0.0	15.844544390655928	0.0
16	3.9469763201155676E-4	0.0	0.0	16.617362354134556	0.0
17	5.920464480173351E-4	0.0	0.0	17.639826569860492	0.0
18	5.920464480173351E-4	0.0	0.0	18.641963857537835	0.0
19	5.920464480173351E-4	0.0	0.0	19.368602198071112	0.0
20	5.920464480173351E-4	0.0	0.0	19.997552874681528	0.0
21	5.920464480173351E-4	0.0	0.0	20.775107209744295	0.0
22	5.920464480173351E-4	0.0	0.0	21.581474471943906	0.0
23	5.920464480173351E-4	0.0	0.0	22.431850520112803	0.0
24	5.920464480173351E-4	0.0	0.0	23.063761428963307	0.0
25	5.920464480173351E-4	0.0	0.0	23.696264384261827	0.0
26	5.920464480173351E-4	0.0	0.0	24.264826323174475	0.0
27	5.920464480173351E-4	0.0	0.0	24.857464817639826	0.0
28	5.920464480173351E-4	0.0	0.0	25.449708614473167	0.0
29	5.920464480173351E-4	0.0	0.0	26.070173291995335	0.0
30	5.920464480173351E-4	0.0	0.0	26.767011961311738	0.0
31	5.920464480173351E-4	0.0	0.0	27.390634219889996	0.0
32	5.920464480173351E-4	0.0	0.0	27.999060619635813	0.0
33	5.920464480173351E-4	0.0	0.0	28.61577566965387	0.0
34	5.920464480173351E-4	0.0	0.0	29.20900621056724	0.0
35	5.920464480173351E-4	0.0	0.0	29.780923079351986	0.0
36	5.920464480173351E-4	0.0	0.0	30.298371674919135	0.0
37	5.920464480173351E-4	0.0	0.0	30.866933613831783	0.0
38	5.920464480173351E-4	0.0	0.0	31.415168624695838	0.0
39	5.920464480173351E-4	0.0	0.0	31.97879684320834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCATCG	25	3.8877064E-5	45.000004	1
TCGCAAG	25	3.8877064E-5	45.000004	1
CTATCGA	20	7.029547E-4	45.000004	41
GCGCGAC	20	7.029547E-4	45.000004	9
ATTCGGT	20	7.029547E-4	45.000004	11
TTCGTAG	25	3.8877064E-5	45.000004	1
CGCGACC	20	7.029547E-4	45.000004	10
GCGTTCG	20	7.029547E-4	45.000004	1
CGTTCGG	25	3.8877064E-5	45.000004	2
ACTAGCG	25	3.8877064E-5	45.000004	1
GTCGTTG	25	3.8877064E-5	45.000004	1
TCGAGCG	20	7.029547E-4	45.000004	1
GGCGCGA	20	7.029547E-4	45.000004	8
TACACGG	40	6.8012014E-9	45.000004	2
GTAAACG	20	7.029547E-4	45.000004	1
TCGTTGG	35	1.2101373E-7	45.0	2
ACTACGG	45	3.8380676E-10	45.0	2
CTCCGGG	30	2.1630913E-6	44.999996	3
CGTTTTT	3655	0.0	43.95349	1
CGTTATT	620	0.0	43.91129	1
>>END_MODULE