FastQCFastQC Report
Sat 14 Jan 2017
SRR2935859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489149
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC50121.0246366649016967Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC40690.8318528710065849No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG38940.7960764511426988No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.7181860741818955No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC18690.3820921641463031Illumina Single End Adapter 1 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT9990.20423224825155523Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT7990.1633449112642569No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG6990.14290124277060773Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC6600.13492821205808456Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT6440.1316572250991007No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC6420.13124835172922772No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT6240.12756849140037085Illumina Single End Adapter 1 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA5390.11019137318076906No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT5260.10753369627659465No Hit
GAATATGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC5170.10569376611216624Illumina Single End Adapter 1 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC5030.10283165252305536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG351.21001E-745.0000042
TTTACGA253.8875078E-545.036
GTGATCG207.0293085E-445.038
CCAACTA207.0293085E-445.035
CGACTGG502.1827873E-1145.02
TCGACGT207.0293085E-445.026
GTAACGG253.8875078E-545.02
CCCATCG253.8875078E-545.034
GGCTACG207.0293085E-445.01
TAACGGG1100.042.9545443
TAATACG1850.041.3513534
CGTTTTT15650.041.118211
TACGGGA3150.040.7142834
CGGGACG451.9244908E-840.06
CTCGAAT900.040.043
AGACGGG1700.039.7058833
CGTAAGG800.039.3752
CGTTATT2400.039.3749961
CGAATGC750.039.045
CGATGAA6350.038.9763819
Produced by FastQC (version 0.11.5)