##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935859.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489149
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0024307521839	33.0	31.0	34.0	30.0	34.0
2	32.13071272761469	33.0	31.0	34.0	30.0	34.0
3	32.06689986077862	33.0	31.0	34.0	30.0	34.0
4	35.72450725648013	37.0	35.0	37.0	33.0	37.0
5	31.94273115144874	37.0	35.0	37.0	0.0	37.0
6	33.77693504433209	37.0	35.0	37.0	17.0	37.0
7	35.45972495088409	37.0	35.0	37.0	32.0	37.0
8	35.693733402296644	37.0	35.0	37.0	33.0	37.0
9	37.65567955776256	39.0	37.0	39.0	35.0	39.0
10	37.31572792748222	39.0	37.0	39.0	34.0	39.0
11	37.24367830661005	39.0	37.0	39.0	34.0	39.0
12	37.26786929953859	39.0	37.0	39.0	34.0	39.0
13	37.231303754070844	39.0	37.0	39.0	34.0	39.0
14	38.4793263402358	40.0	38.0	41.0	34.0	41.0
15	38.57769105119299	40.0	38.0	41.0	34.0	41.0
16	38.55805899633854	40.0	38.0	41.0	34.0	41.0
17	38.47048854234599	40.0	38.0	41.0	34.0	41.0
18	38.41067854580097	40.0	38.0	41.0	34.0	41.0
19	38.273254161819814	40.0	37.0	41.0	34.0	41.0
20	38.208151299501786	40.0	37.0	41.0	34.0	41.0
21	38.230860126464535	40.0	37.0	41.0	34.0	41.0
22	38.29206846993452	40.0	37.0	41.0	34.0	41.0
23	38.235261648291214	40.0	37.0	41.0	34.0	41.0
24	38.25290248983438	40.0	37.0	41.0	34.0	41.0
25	38.052474808289496	40.0	37.0	41.0	34.0	41.0
26	38.06446706422788	40.0	37.0	41.0	34.0	41.0
27	37.9998262288178	40.0	37.0	41.0	34.0	41.0
28	37.94305007267724	40.0	37.0	41.0	33.0	41.0
29	37.91073476588933	40.0	37.0	41.0	33.0	41.0
30	37.75427732654058	40.0	36.0	41.0	33.0	41.0
31	37.68157964137717	40.0	36.0	41.0	33.0	41.0
32	37.54749370846102	40.0	36.0	41.0	33.0	41.0
33	37.44168545780529	40.0	36.0	41.0	33.0	41.0
34	37.22248639984954	40.0	36.0	41.0	32.0	41.0
35	37.188194190318285	40.0	36.0	41.0	32.0	41.0
36	37.128014163373535	40.0	36.0	41.0	32.0	41.0
37	37.0489216987053	40.0	36.0	41.0	32.0	41.0
38	36.91046286509837	40.0	36.0	41.0	31.0	41.0
39	36.84655186865352	40.0	35.0	41.0	31.0	41.0
40	36.77070585854208	40.0	35.0	41.0	31.0	41.0
41	36.66620191393624	40.0	35.0	41.0	30.0	41.0
42	36.66889434507686	40.0	35.0	41.0	31.0	41.0
43	36.62498747825305	40.0	35.0	41.0	31.0	41.0
44	36.62203950125626	39.0	35.0	41.0	31.0	41.0
45	36.59042336793083	39.0	35.0	41.0	31.0	41.0
46	36.4679044626484	39.0	35.0	41.0	30.0	41.0
47	36.29472614683869	39.0	35.0	41.0	30.0	41.0
48	36.32041361630096	39.0	35.0	41.0	30.0	41.0
49	36.32122318557331	39.0	35.0	41.0	30.0	41.0
50	36.19052885726026	39.0	35.0	41.0	30.0	41.0
51	35.268055336921876	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	16.0
10	26.0
11	19.0
12	13.0
13	10.0
14	8.0
15	16.0
16	35.0
17	63.0
18	124.0
19	287.0
20	597.0
21	1137.0
22	1772.0
23	2451.0
24	2940.0
25	3759.0
26	4625.0
27	5030.0
28	5194.0
29	5602.0
30	6577.0
31	8571.0
32	11123.0
33	15500.0
34	24926.0
35	33655.0
36	35888.0
37	56419.0
38	96104.0
39	166526.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.990585690658676	19.477091847269442	22.785286282911752	12.747036179160135
2	32.45841246736679	27.356899431461578	23.984920750119084	16.199767351052543
3	30.26623789479279	27.404942052421653	27.10646449241438	15.222355560371176
4	26.51830014985209	24.06076676023052	31.730617868992884	17.690315220924504
5	24.489470488542345	35.51085661015355	22.94474689716221	17.054926004141887
6	24.104311774121996	35.91012145583452	25.114024561023328	14.871542209020156
7	77.31263888917283	4.741295597047117	13.725879026636056	4.220186487143999
8	78.4738392596121	7.642047719610997	9.697045276592613	4.187067744184287
9	72.6698817742651	6.948394047621481	10.754596247769085	9.627127930344333
10	36.233744728088986	33.617772907641644	17.36014997475207	12.788332389517304
11	25.391036269112277	23.45072769238003	30.651805482583015	20.506430555924677
12	22.97991000697129	21.765351661763592	32.592318496000196	22.66241983526492
13	23.751862929291484	22.457983150328428	35.396576503274055	18.393577417106037
14	19.436204510282142	28.837634340456592	30.564715454800073	21.161445694461197
15	17.77392982506353	25.18148866705237	35.763335916050124	21.28124559183398
16	20.852132990152285	26.35638629538239	30.076111777801856	22.71536893666347
17	20.913463995633233	26.2729761279283	31.125076408210994	21.688483468227474
18	21.163898934680432	24.543237336680644	31.602844940907577	22.690018787731344
19	20.627252636722144	26.753402337529053	29.297003571508885	23.322341454239915
20	23.42292430322867	27.127725907647772	30.82005687428575	18.629292914837812
21	22.260701749364713	28.229639639455463	30.77855622724364	18.731102383936182
22	21.14836174662526	23.132215337248976	32.37132243958385	23.348100476541912
23	20.404212213456432	26.93555542380747	31.531292101179805	21.128940261556295
24	20.89015821355047	25.6970779864622	31.11424126390936	22.298522536077964
25	20.1474397371762	29.94670335623706	28.164628773645656	21.74122813294109
26	20.376408824305067	26.199378921351162	29.999652457635605	23.42455979670816
27	22.182606935718972	26.26663859069527	29.131409856710327	22.41934461687543
28	18.307305136062833	27.643110790372667	31.416807557615368	22.632776515949125
29	21.904573044205343	24.9396400687725	30.545498406416037	22.610288480606116
30	21.362611392438705	27.264902923240157	29.81238845423378	21.560097230087354
31	22.25027547843295	27.780083369280117	26.565320587387482	23.404320564899447
32	23.399209647776033	30.064458886760477	26.386234051383116	20.150097414080374
33	23.255490658265682	26.173619899049168	26.148065313432106	24.422824129253048
34	21.794586107709513	28.300579169128426	28.07406332221879	21.83077140094327
35	20.729879852560263	28.05096197682097	27.6040633835498	23.615094787068973
36	22.420571236985047	29.42048332921053	26.006595127456052	22.152350306348374
37	19.63389478461573	29.12486788279236	27.915420454708073	23.325816877883835
38	20.440397506690193	30.126198765611296	25.9945333630448	23.438870364653717
39	21.326630535889883	29.326033580769867	27.159413593812925	22.18792228952732
40	23.2242118454704	26.339213613847722	28.589243768258754	21.847330772423128
41	20.01108046832356	28.4326452675974	26.79858284490002	24.75769141917902
42	20.267852944603792	27.65558142815379	28.76751255752337	23.30905306971904
43	22.012311177166875	25.814424643615748	27.121797243784613	25.05146693543276
44	21.639214227157776	26.064655145978016	28.08612508663004	24.21000554023416
45	21.575838854827463	25.60998795867926	27.727338704566503	25.086834481926772
46	24.21368540056302	26.978691564329072	26.75442452095374	22.053198514154175
47	19.608544635683607	26.92635577298533	31.14122690632098	22.323872685010087
48	20.313033451974757	27.300679343104044	28.39932208795275	23.98696511696845
49	21.11585631372036	25.056986725926045	31.071922870127505	22.755234090226086
50	21.08069320391128	24.989522619897006	29.911335809743044	24.01844836644867
51	20.387652841976575	24.6051816522164	27.99739956536761	27.00976594043942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	380.5
2	602.0
3	3315.5
4	6029.0
5	3816.5
6	1604.0
7	1569.5
8	1535.0
9	1572.5
10	1610.0
11	1561.0
12	1512.0
13	1504.0
14	1496.0
15	1451.5
16	1407.0
17	1390.0
18	1373.0
19	1382.0
20	1391.0
21	1655.0
22	1919.0
23	1981.5
24	2044.0
25	2442.5
26	3707.5
27	4574.0
28	5548.0
29	6522.0
30	7604.5
31	8687.0
32	10206.5
33	11726.0
34	12869.0
35	14012.0
36	15440.5
37	16869.0
38	18242.5
39	19616.0
40	21626.0
41	23636.0
42	25711.0
43	27786.0
44	31477.5
45	35169.0
46	45964.0
47	56759.0
48	51664.0
49	46569.0
50	45676.5
51	44784.0
52	39920.0
53	35056.0
54	31245.0
55	27434.0
56	24651.5
57	21869.0
58	19762.5
59	17656.0
60	15569.5
61	13483.0
62	11876.5
63	10270.0
64	8765.0
65	7260.0
66	6025.0
67	4790.0
68	4091.5
69	3393.0
70	2852.5
71	2312.0
72	1887.5
73	1463.0
74	1250.5
75	778.5
76	519.0
77	359.0
78	199.0
79	137.5
80	76.0
81	66.5
82	57.0
83	42.5
84	28.0
85	19.0
86	10.0
87	6.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.53591816406727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67658979725873	26.187700191120637
2	9.480382858646632	6.92748984575079
3	3.8843752734705985	4.257576513301446
4	2.3914919052540053	3.4950141016155865
5	1.678627550356496	3.0665099403886824
6	1.371727477869701	3.007039372490988
7	1.1490194068122068	2.9386335311351135
8	0.9902869314599716	2.8944833829413454
9	0.9145769629795313	3.00734181667452
>10	6.386564603890174	36.27670247204152
>50	0.046156237254899306	1.1717082634560625
>100	0.021083713313966298	1.459014127629721
>500	0.006268130985233223	1.4889938699782768
>1k	0.002279320358266627	2.778330983621379
>5k	5.698300895666567E-4	1.0434615878539044
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	5012	1.0246366649016967	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	4069	0.8318528710065849	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	3894	0.7960764511426988	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3513	0.7181860741818955	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	1869	0.3820921641463031	Illumina Single End Adapter 1 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	999	0.20423224825155523	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	799	0.1633449112642569	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	699	0.14290124277060773	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	660	0.13492821205808456	Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	644	0.1316572250991007	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	642	0.13124835172922772	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	624	0.12756849140037085	Illumina Single End Adapter 1 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	539	0.11019137318076906	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT	526	0.10753369627659465	No Hit
GAATATGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	517	0.10569376611216624	Illumina Single End Adapter 1 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	503	0.10283165252305536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0443668493649175E-4	0.0	0.0	0.1341104653183386	0.0
2	2.0443668493649175E-4	0.0	0.0	0.7821747565670174	0.0
3	2.0443668493649175E-4	0.0	0.0	1.0986427448487066	0.0
4	2.0443668493649175E-4	0.0	0.0	1.620160728121697	0.0
5	2.0443668493649175E-4	0.0	0.0	3.5044536531813413	0.0
6	2.0443668493649175E-4	0.0	0.0	4.3837358350931925	0.0
7	2.0443668493649175E-4	0.0	0.0	5.185740950099049	0.0
8	2.0443668493649175E-4	0.0	0.0	6.223461562836682	0.0
9	2.0443668493649175E-4	0.0	0.0	6.616388871284618	0.0
10	2.0443668493649175E-4	0.0	0.0	8.268032848886536	0.0
11	2.0443668493649175E-4	0.0	0.0	9.296758247486961	0.0
12	2.0443668493649175E-4	0.0	0.0	11.135257355120832	0.0
13	2.0443668493649175E-4	0.0	0.0	11.56784538044645	0.0
14	2.0443668493649175E-4	0.0	0.0	11.765740091464973	0.0
15	2.0443668493649175E-4	0.0	0.0	12.13249950424104	0.0
16	2.0443668493649175E-4	0.0	0.0	12.664034885075917	0.0
17	2.0443668493649175E-4	0.0	0.0	13.323752067365977	0.0
18	2.0443668493649175E-4	0.0	0.0	13.975905092313385	0.0
19	2.0443668493649175E-4	0.0	0.0	14.587375216958431	0.0
20	6.133100548094753E-4	0.0	0.0	15.02630077951708	0.0
21	6.133100548094753E-4	0.0	0.0	15.498958395090249	0.0
22	6.133100548094753E-4	0.0	0.0	16.080171890364696	0.0
23	6.133100548094753E-4	0.0	0.0	16.616818188322984	0.0
24	6.133100548094753E-4	0.0	0.0	17.049201776963667	0.0
25	6.133100548094753E-4	0.0	0.0	17.424343093822127	0.0
26	6.133100548094753E-4	0.0	0.0	17.787013772899464	0.0
27	6.133100548094753E-4	0.0	0.0	18.17932777129259	0.0
28	6.133100548094753E-4	0.0	0.0	18.537500843301327	0.0
29	6.133100548094753E-4	0.0	0.0	18.917957513968137	0.0
30	6.133100548094753E-4	0.0	0.0	19.414534221678874	0.0
31	6.133100548094753E-4	0.0	0.0	19.801941739633527	0.0
32	6.133100548094753E-4	0.0	0.0	20.19854890841032	0.0
33	6.133100548094753E-4	0.0	0.0	20.580641072556624	0.0
34	6.133100548094753E-4	0.0	0.0	20.981541411717085	0.0
35	6.133100548094753E-4	0.0	0.0	21.405134222905495	0.0
36	6.133100548094753E-4	0.0	0.0	21.798470404723304	0.0
37	6.133100548094753E-4	0.0	0.0	22.183220245773782	0.0
38	6.133100548094753E-4	0.0	0.0	22.57778304770121	0.0
39	6.133100548094753E-4	0.0	0.0	22.996673815136084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	35	1.21001E-7	45.000004	2
TTTACGA	25	3.8875078E-5	45.0	36
GTGATCG	20	7.0293085E-4	45.0	38
CCAACTA	20	7.0293085E-4	45.0	35
CGACTGG	50	2.1827873E-11	45.0	2
TCGACGT	20	7.0293085E-4	45.0	26
GTAACGG	25	3.8875078E-5	45.0	2
CCCATCG	25	3.8875078E-5	45.0	34
GGCTACG	20	7.0293085E-4	45.0	1
TAACGGG	110	0.0	42.954544	3
TAATACG	185	0.0	41.351353	4
CGTTTTT	1565	0.0	41.11821	1
TACGGGA	315	0.0	40.714283	4
CGGGACG	45	1.9244908E-8	40.0	6
CTCGAAT	90	0.0	40.0	43
AGACGGG	170	0.0	39.705883	3
CGTAAGG	80	0.0	39.375	2
CGTTATT	240	0.0	39.374996	1
CGAATGC	75	0.0	39.0	45
CGATGAA	635	0.0	38.97638	19
>>END_MODULE