FastQCFastQC Report
Sat 14 Jan 2017
SRR2935856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634641
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC138382.18044532263122No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94031.4816250447103165No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG54140.8530807180752583No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC49930.7867440017269606No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT37970.5982910023146945No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC29720.46829624937563125No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT25640.40400793519485817No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT24910.3925053691772199No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC20980.33058059595897527No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG17660.2782675559883462No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT15260.2404509005878914No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT14160.22311826686268302No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC13600.21429438060257688No Hit
GAATATGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC12530.19743445506987417No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT10550.166235714364499No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.16229647942695163No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC10020.15788453629689855No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT8590.1353521124541276No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT7850.1236919770389874Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTG750.045.0000045
CGATCGA600.045.00000441
GGCAATC207.030895E-445.08
TAATACG2150.043.9534874
CGTTTTT52100.043.9203451
CGTTATT6850.042.700731
TACGGCT6250.042.127
TAACGGG1550.042.096773
CGTTTCT2300.042.0652161
GATCGAA650.041.5384642
CGAATAT603.6379788E-1241.25000414
TACGGGT501.0804797E-940.54
TAGTGCG501.0804797E-940.51
CGTTCTG2750.040.0909081
ACGGGAG3400.039.7058835
GTAACGG850.039.7058832
ACGGCTG6650.039.5864688
CCGATGA7200.039.37518
CGTAAGG800.039.3752
GAATCTG16650.038.918921
Produced by FastQC (version 0.11.5)