##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935856.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634641
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.961105569920633	33.0	31.0	34.0	30.0	34.0
2	32.0976347257741	33.0	31.0	34.0	30.0	34.0
3	31.95350757357309	33.0	31.0	34.0	30.0	34.0
4	35.67450731988636	37.0	35.0	37.0	33.0	37.0
5	32.06113535053676	37.0	35.0	37.0	0.0	37.0
6	33.79080298940661	37.0	35.0	37.0	17.0	37.0
7	35.40928808570515	37.0	35.0	37.0	32.0	37.0
8	35.59511913034298	37.0	35.0	37.0	33.0	37.0
9	37.56270237819491	39.0	37.0	39.0	35.0	39.0
10	37.280353774811275	39.0	37.0	39.0	34.0	39.0
11	37.23570175894719	39.0	37.0	39.0	34.0	39.0
12	37.216333328606254	39.0	37.0	39.0	34.0	39.0
13	37.17542358593283	39.0	37.0	39.0	33.0	39.0
14	38.388020944124314	40.0	38.0	41.0	34.0	41.0
15	38.418773763434764	40.0	38.0	41.0	34.0	41.0
16	38.436560197024775	40.0	38.0	41.0	34.0	41.0
17	38.387252005464504	40.0	38.0	41.0	34.0	41.0
18	38.19021462527634	40.0	37.0	41.0	34.0	41.0
19	37.97350470581006	40.0	37.0	41.0	34.0	41.0
20	37.81949480099773	40.0	35.0	41.0	33.0	41.0
21	37.83979131508995	40.0	36.0	41.0	33.0	41.0
22	37.90406387233097	40.0	35.0	41.0	34.0	41.0
23	37.90710496170276	40.0	35.0	41.0	34.0	41.0
24	37.88210027401318	40.0	35.0	41.0	34.0	41.0
25	37.69600608848152	40.0	35.0	41.0	33.0	41.0
26	37.64813020274455	40.0	35.0	41.0	33.0	41.0
27	37.63874852081728	40.0	35.0	41.0	33.0	41.0
28	37.53824445631467	40.0	35.0	41.0	33.0	41.0
29	37.442108215510814	40.0	35.0	41.0	33.0	41.0
30	37.2224801108028	39.0	35.0	41.0	33.0	41.0
31	37.041650318841675	39.0	35.0	41.0	32.0	41.0
32	36.79073050748376	39.0	35.0	41.0	31.0	41.0
33	36.5032356875777	39.0	35.0	41.0	30.0	41.0
34	36.1496531109714	39.0	35.0	41.0	29.0	41.0
35	36.067483821562114	40.0	35.0	41.0	27.0	41.0
36	35.91612580970974	39.0	35.0	41.0	26.0	41.0
37	35.78161669353225	39.0	35.0	41.0	25.0	41.0
38	35.675575640401426	39.0	35.0	41.0	24.0	41.0
39	35.54154395949836	39.0	35.0	41.0	24.0	41.0
40	35.48894729461223	39.0	35.0	41.0	24.0	41.0
41	35.34377703299976	39.0	35.0	41.0	23.0	41.0
42	35.34035777707397	39.0	35.0	41.0	23.0	41.0
43	35.29842540901076	39.0	35.0	41.0	23.0	41.0
44	35.2993629469259	39.0	35.0	41.0	23.0	41.0
45	35.29019240799129	39.0	35.0	41.0	23.0	41.0
46	35.182403910242165	39.0	35.0	41.0	23.0	41.0
47	35.00882231056613	38.0	35.0	41.0	23.0	41.0
48	35.01318225579501	38.0	35.0	41.0	23.0	41.0
49	35.009337562495965	39.0	35.0	41.0	23.0	41.0
50	34.91490149549115	38.0	35.0	41.0	22.0	41.0
51	33.91776610713774	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	14.0
10	23.0
11	14.0
12	14.0
13	16.0
14	16.0
15	32.0
16	53.0
17	91.0
18	237.0
19	489.0
20	980.0
21	1810.0
22	2770.0
23	4162.0
24	5903.0
25	8722.0
26	11367.0
27	11855.0
28	10876.0
29	10053.0
30	10794.0
31	12600.0
32	16408.0
33	22516.0
34	35086.0
35	43635.0
36	46736.0
37	69275.0
38	113836.0
39	194113.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16039934388103	17.287726446920384	19.803164308640632	18.74870990055795
2	37.30739110772862	27.256827088070263	20.40807322565041	15.027708578550708
3	27.024097087959966	27.575432409819093	31.111762397954116	14.288708104266822
4	24.054544222639258	21.35128363909675	37.59826421551712	16.995907922746873
5	23.290175075357563	32.45850803840281	27.149679897768976	17.10163698847065
6	22.40589561657693	32.38807451771947	31.24884777378077	13.957182091922835
7	69.91968687809327	4.5975598803102855	20.763707355812183	4.719045885784247
8	69.68664173918798	7.693956110620021	17.389201138911606	5.230201011280393
9	64.08883132353567	5.903652616203491	18.22274325169663	11.784772808564211
10	34.2037466851338	26.04637897646071	25.329753356622092	14.420120981783402
11	26.603701935424912	21.436686252542774	33.06262910842508	18.896982703607236
12	22.46923851437269	19.71902225037462	36.65205998351824	21.159679251734445
13	23.106606727267856	20.838395250228082	39.36367174512835	16.691326277375712
14	18.64109000206416	26.848879917937857	35.23440811419369	19.27562196580429
15	16.493576683510835	24.07802206286704	40.43151955199869	18.99688170162344
16	20.064256800301273	25.892906383293862	33.35901714512614	20.683819671278723
17	20.17360996216759	25.796631481420206	33.626885120879365	20.402873435532847
18	20.4822884118738	24.34778087139028	33.974640781166045	21.19528993556987
19	19.548847301072573	26.133672422676756	31.513249222788946	22.80423105346172
20	21.538633652726503	27.019370006034908	33.52446501250313	17.917531328735457
21	20.436908425393256	27.86016031110502	34.69552077473722	17.007410488764513
22	19.6175475583834	22.233829834504863	35.63684035541353	22.511782251698204
23	19.646225188728746	25.63921965331581	35.146799529182644	19.5677556287728
24	21.089245731051097	24.426250431346226	33.65304164086468	20.831462196738
25	19.77795320504033	29.417576236013748	30.959235221172282	19.84523533777364
26	19.35472180335024	24.888559043616784	32.853849656735065	22.902869496297907
27	23.226201899971795	24.039417560479073	31.227733474515514	21.50664706503362
28	18.437352771094208	25.704138245086593	33.46222510048988	22.396283883329314
29	21.650980633145352	25.532860310002032	32.01526532322998	20.80089373362263
30	23.092898189685194	25.46857199582126	32.25949788935792	19.17903192513563
31	22.548495921316146	22.93469851459329	32.93405248006353	21.582753084027033
32	26.506166478371235	24.616279124733513	30.48243022433155	18.3951241725637
33	23.685516693689816	22.903027065695408	29.7216851731924	23.68977106742237
34	20.94050021980931	24.659610709046532	33.872220672789815	20.527668398354347
35	25.06346107484389	24.75525533333018	30.102845545749485	20.078438046076442
36	21.911285277818482	29.55749786099543	29.55749786099543	18.97371900019066
37	22.893257763050293	25.375921190090146	32.074353847293196	19.656467199566368
38	20.96807486437214	25.70240498171407	29.476192051884453	23.853328102029337
39	23.36549324736347	24.77400609163291	30.390252126792944	21.47024853421068
40	23.350051446408283	23.615398311801474	31.926081044243908	21.108469197546327
41	20.02061007719325	27.204514048099632	30.889589547476447	21.88528632723067
42	22.588518548281627	25.11593168421202	32.233656508167606	20.061893259338746
43	23.032549110441966	25.486377337738976	29.690486432487027	21.79058711933203
44	22.11801632734097	23.518335562940308	30.350859777417472	24.012788332301255
45	21.31724864923634	22.203734079582	29.752096066910266	26.72692120427139
46	24.342423511875218	24.70199687697454	28.817551970326534	22.138027640823708
47	19.986102379140334	24.196356680390963	34.013402852951515	21.804138087517195
48	20.741332501366916	24.345417330427754	31.110029134581595	23.803221033623736
49	21.465521452285625	22.138027640823708	34.23069105210663	22.165759854784042
50	20.029276394055852	23.317119442330387	33.39257942679404	23.261024736819714
51	19.924807883512095	22.14622124949381	31.1936984846551	26.73527238233899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	429.0
2	701.0
3	8573.0
4	16445.0
5	10922.5
6	5400.0
7	5086.5
8	4773.0
9	4788.5
10	4804.0
11	4772.0
12	4740.0
13	4585.0
14	4430.0
15	4187.5
16	3945.0
17	3774.5
18	3604.0
19	3476.5
20	3349.0
21	3319.0
22	3289.0
23	3455.0
24	3621.0
25	3718.5
26	4535.0
27	5254.0
28	6295.5
29	7337.0
30	8340.0
31	9343.0
32	10968.0
33	12593.0
34	13947.0
35	15301.0
36	17332.0
37	19363.0
38	20880.0
39	22397.0
40	24164.5
41	25932.0
42	27855.5
43	29779.0
44	34688.0
45	39597.0
46	62085.5
47	84574.0
48	71612.0
49	58650.0
50	57710.5
51	56771.0
52	49951.0
53	43131.0
54	38605.5
55	34080.0
56	31087.0
57	28094.0
58	24754.5
59	21415.0
60	18977.5
61	16540.0
62	14815.5
63	13091.0
64	11442.5
65	9794.0
66	8092.5
67	6391.0
68	5521.5
69	4652.0
70	3787.0
71	2922.0
72	2544.5
73	2167.0
74	1582.0
75	858.0
76	719.0
77	558.5
78	398.0
79	268.0
80	138.0
81	97.0
82	56.0
83	56.0
84	56.0
85	40.0
86	24.0
87	14.0
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	634641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.2157784498596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15787109675007	29.823165143401482
2	9.33704530121265	7.509930904197412
3	4.06466077482588	4.903904915826969
4	2.4341387354227297	3.9156313639992764
5	1.7181249263779015	3.4547865694197517
6	1.359874204973351	3.2813039848132353
7	1.087362507074206	3.0610390775426115
8	0.9025748955610745	2.903820162743147
9	0.7271848475292917	2.6319874248299637
>10	4.145199415347297	26.056501504699987
>50	0.03457112451798039	0.9307051120428215
>100	0.02185530851161565	1.528056705314532
>500	0.0027815847196601736	0.7339849871898814
>1k	0.005563169439320347	4.685965987234681
>5k	7.947384913314782E-4	2.3678326921821187
>10k+	3.973692456657391E-4	2.2113834645620676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	13838	2.18044532263122	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9403	1.4816250447103165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	5414	0.8530807180752583	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	4993	0.7867440017269606	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	3797	0.5982910023146945	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	2972	0.46829624937563125	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	2564	0.40400793519485817	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTT	2491	0.3925053691772199	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	2098	0.33058059595897527	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTG	1766	0.2782675559883462	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	1526	0.2404509005878914	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT	1416	0.22311826686268302	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	1360	0.21429438060257688	No Hit
GAATATGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	1253	0.19743445506987417	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	1055	0.166235714364499	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1030	0.16229647942695163	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	1002	0.15788453629689855	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	859	0.1353521124541276	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT	785	0.1236919770389874	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.302775900075791E-4	0.0	0.0	0.127316073181531	0.0
2	6.302775900075791E-4	0.0	0.0	0.8387419029025859	0.0
3	6.302775900075791E-4	0.0	0.0	1.3037291949306773	0.0
4	6.302775900075791E-4	0.0	0.0	2.3077613958127507	0.0
5	6.302775900075791E-4	0.0	0.0	5.586150280237174	0.0
6	6.302775900075791E-4	0.0	0.0	7.422148899929251	0.0
7	6.302775900075791E-4	0.0	0.0	8.705394073184682	0.0
8	6.302775900075791E-4	0.0	0.0	10.163383708269714	0.0
9	6.302775900075791E-4	0.0	0.0	10.907583972671164	0.0
10	6.302775900075791E-4	0.0	0.0	12.81165257208406	0.0
11	6.302775900075791E-4	0.0	0.0	13.846410805479003	0.0
12	6.302775900075791E-4	0.0	0.0	15.52342190309167	0.0
13	6.302775900075791E-4	0.0	0.0	16.010626480167527	0.0
14	6.302775900075791E-4	0.0	0.0	16.32245631782378	0.0
15	6.302775900075791E-4	0.0	0.0	16.707398355920905	0.0
16	6.302775900075791E-4	0.0	0.0	17.16687071903643	0.0
17	6.302775900075791E-4	0.0	0.0	17.685274036817667	0.0
18	6.302775900075791E-4	0.0	0.0	18.227155194826683	0.0
19	6.302775900075791E-4	0.0	0.0	18.740358722490353	0.0
20	6.302775900075791E-4	0.0	0.0	19.1268764545625	0.0
21	7.878469875094739E-4	0.0	0.0	19.542229386377496	0.0
22	7.878469875094739E-4	0.0	0.0	20.052754234283633	0.0
23	7.878469875094739E-4	0.0	0.0	20.521838330646776	0.0
24	7.878469875094739E-4	0.0	0.0	20.89307183116124	0.0
25	7.878469875094739E-4	0.0	0.0	21.239409366870404	0.0
26	7.878469875094739E-4	0.0	0.0	21.580231973667	0.0
27	7.878469875094739E-4	0.0	0.0	21.979827965731808	0.0
28	7.878469875094739E-4	0.0	0.0	22.337825636856113	0.0
29	7.878469875094739E-4	0.0	0.0	22.712210525320614	0.0
30	7.878469875094739E-4	0.0	0.0	23.20004537998648	0.0
31	7.878469875094739E-4	0.0	0.0	23.573957560258478	0.0
32	7.878469875094739E-4	0.0	0.0	23.974814107503295	0.0
33	7.878469875094739E-4	0.0	0.0	24.34778087139028	0.0
34	7.878469875094739E-4	0.0	0.0	24.734613742257434	0.0
35	7.878469875094739E-4	0.0	0.0	25.13326431793723	0.0
36	7.878469875094739E-4	0.0	0.0	25.502922124476672	0.0
37	7.878469875094739E-4	0.0	0.0	25.8962153406414	0.0
38	7.878469875094739E-4	0.0	0.0	26.302901955593793	0.0
39	7.878469875094739E-4	0.0	0.0	26.84163172565277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTG	75	0.0	45.000004	5
CGATCGA	60	0.0	45.000004	41
GGCAATC	20	7.030895E-4	45.0	8
TAATACG	215	0.0	43.953487	4
CGTTTTT	5210	0.0	43.920345	1
CGTTATT	685	0.0	42.70073	1
TACGGCT	625	0.0	42.12	7
TAACGGG	155	0.0	42.09677	3
CGTTTCT	230	0.0	42.065216	1
GATCGAA	65	0.0	41.53846	42
CGAATAT	60	3.6379788E-12	41.250004	14
TACGGGT	50	1.0804797E-9	40.5	4
TAGTGCG	50	1.0804797E-9	40.5	1
CGTTCTG	275	0.0	40.090908	1
ACGGGAG	340	0.0	39.705883	5
GTAACGG	85	0.0	39.705883	2
ACGGCTG	665	0.0	39.586468	8
CCGATGA	720	0.0	39.375	18
CGTAAGG	80	0.0	39.375	2
GAATCTG	1665	0.0	38.91892	1
>>END_MODULE