##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594796
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976998836575902	33.0	31.0	34.0	30.0	34.0
2	32.088690912514544	33.0	31.0	34.0	30.0	34.0
3	32.05578719426492	33.0	31.0	34.0	30.0	34.0
4	35.71952568611759	37.0	35.0	37.0	35.0	37.0
5	31.922997128427227	37.0	35.0	37.0	0.0	37.0
6	33.73540003631497	37.0	35.0	37.0	17.0	37.0
7	35.385012676615176	37.0	35.0	37.0	32.0	37.0
8	35.56045938439398	37.0	35.0	37.0	33.0	37.0
9	37.49465194789474	39.0	37.0	39.0	35.0	39.0
10	37.16860906932797	39.0	37.0	39.0	34.0	39.0
11	37.19341085010659	39.0	37.0	39.0	34.0	39.0
12	37.12431825365336	39.0	37.0	39.0	34.0	39.0
13	37.01787167364945	39.0	37.0	39.0	33.0	39.0
14	38.24270842440097	40.0	37.0	41.0	33.0	41.0
15	38.289131063423426	40.0	37.0	41.0	34.0	41.0
16	38.34663145011063	40.0	37.0	41.0	34.0	41.0
17	38.20888170061668	40.0	37.0	41.0	33.0	41.0
18	38.1526741941775	40.0	37.0	41.0	34.0	41.0
19	38.03634691558114	40.0	37.0	41.0	34.0	41.0
20	37.92804591826442	40.0	37.0	41.0	33.0	41.0
21	38.042483473325305	40.0	37.0	41.0	33.0	41.0
22	38.07746017121837	40.0	37.0	41.0	34.0	41.0
23	38.00875762446284	40.0	37.0	41.0	34.0	41.0
24	37.99528241615613	40.0	36.0	41.0	33.0	41.0
25	37.87201998668451	40.0	36.0	41.0	33.0	41.0
26	37.82853953288186	40.0	36.0	41.0	33.0	41.0
27	37.76554818795015	40.0	36.0	41.0	33.0	41.0
28	37.690339208737115	40.0	36.0	41.0	33.0	41.0
29	37.678582236598764	40.0	36.0	41.0	33.0	41.0
30	37.52238750764968	40.0	36.0	41.0	33.0	41.0
31	37.57869420776199	40.0	36.0	41.0	33.0	41.0
32	37.360839682849246	40.0	36.0	41.0	32.0	41.0
33	37.23426855594187	40.0	36.0	41.0	32.0	41.0
34	37.06245838909475	40.0	36.0	41.0	31.0	41.0
35	37.00727140061466	40.0	36.0	41.0	31.0	41.0
36	36.982725169637995	40.0	35.0	41.0	31.0	41.0
37	36.92590736992179	40.0	35.0	41.0	31.0	41.0
38	36.83214076759091	40.0	35.0	41.0	31.0	41.0
39	36.63019421784948	40.0	35.0	41.0	31.0	41.0
40	36.505877645444826	39.0	35.0	41.0	30.0	41.0
41	36.45994256854451	39.0	35.0	41.0	30.0	41.0
42	36.43868486001924	39.0	35.0	41.0	30.0	41.0
43	36.404044411865584	39.0	35.0	41.0	30.0	41.0
44	36.45201043719191	39.0	35.0	41.0	30.0	41.0
45	36.46284272254689	39.0	35.0	41.0	30.0	41.0
46	36.31814100969072	39.0	35.0	41.0	30.0	41.0
47	36.12009159442901	39.0	35.0	41.0	30.0	41.0
48	36.12125501852736	39.0	35.0	41.0	30.0	41.0
49	36.09565464461765	39.0	35.0	41.0	29.0	41.0
50	36.00108776790698	39.0	35.0	41.0	29.0	41.0
51	34.912065649399125	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	19.0
10	24.0
11	18.0
12	13.0
13	15.0
14	13.0
15	20.0
16	42.0
17	86.0
18	146.0
19	334.0
20	787.0
21	1632.0
22	2752.0
23	3610.0
24	4253.0
25	4887.0
26	5328.0
27	5662.0
28	6128.0
29	7060.0
30	8704.0
31	11348.0
32	15035.0
33	21391.0
34	34429.0
35	44442.0
36	44485.0
37	68028.0
38	113702.0
39	190245.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.781612519250295	18.19581839824074	20.819911364568693	10.202657717940268
2	31.77543224904001	30.571490057095208	21.344797207782165	16.308280486082623
3	29.953967410675258	31.3320869676326	23.67988352309027	15.034062098601876
4	26.976980342840235	22.87725539512707	31.909259645323772	18.236504616708924
5	26.990094082677086	34.8134486445773	20.376566083161286	17.81989118958433
6	23.25267822917437	38.909642970026695	23.875244621685418	13.962434179113512
7	74.0563151063558	5.342335859689708	16.10602626782964	4.495322766124856
8	72.84178104761969	11.775297749144245	10.127505901182927	5.255415302053141
9	67.30341158985603	7.255765001782123	11.289921250311032	14.150902158050826
10	38.59928446055454	25.369202213868284	19.856051486560098	16.175461839017075
11	33.065118124533456	24.241588712768745	23.712499747812696	18.980793414885103
12	26.86013355839649	21.605390755822164	28.50842978096692	23.026045904814424
13	26.49866508853456	22.95610595901788	32.53754228340473	18.007686669042833
14	21.436425261770424	30.023234856992985	27.090128380150507	21.450211501086088
15	19.094445826804485	24.611799675855252	34.88053046758889	21.413224029751376
16	21.61867262052872	24.88987821034439	28.16764067007848	25.32380849904841
17	21.185414831303508	27.25287325402323	29.09821182388584	22.46350009078743
18	22.281925231507945	24.407023584556722	29.56341333835466	23.74763784558067
19	21.947524865668232	26.78716736494529	26.285987128359977	24.979320641026504
20	25.328011620790996	25.886858687684516	30.068460446943153	18.716669244581336
21	23.313875681746346	30.399330190519102	27.245307634886583	19.041486492847966
22	20.50803973126921	23.99377265482619	30.045931714402922	25.45225589950168
23	22.53125441327783	28.029273902312724	28.596695337561112	20.842776346848332
24	22.249813381394627	25.919979287016055	27.680078547939125	24.150128783650192
25	20.263418045851015	31.893119657832266	25.653165118797034	22.19029717751969
26	20.072091944128744	25.216881081917165	30.37528833415154	24.335738639802553
27	23.90248085057734	26.018500460662143	26.889555410594557	23.18946327816596
28	18.87168037444771	26.973449720576465	30.143444138830795	24.01142576614503
29	22.13414347103881	24.28815930167654	30.27239591389317	23.30530131339148
30	20.824786985790087	27.90385274951412	28.101399471415412	23.169960793280385
31	23.574973604395456	24.607260304373263	24.467716662519585	27.350049428711692
32	26.802466728088287	27.779776595673138	25.045561839689572	20.372194836549003
33	23.39423936946449	27.104250869205575	23.95611268401267	25.54539707731727
34	23.93408832608155	25.44603527932266	27.323990073907694	23.2958863206881
35	21.918607388079277	27.2409363882743	25.823979986415512	25.01647623723092
36	20.215502457985597	31.641604852756238	25.581039549694353	22.561853139563816
37	23.098003349047406	26.213693434387586	26.59752251192005	24.090780704644953
38	20.615639647879274	29.267009193067878	23.38650562545814	26.73084553359471
39	26.271192139826095	26.594496264265395	25.143242388987147	21.991069206921367
40	21.99728982710038	27.50892743058124	28.022380782654892	22.47140195966348
41	21.101016146712485	28.467743562498736	25.65081137062119	24.780428920167587
42	20.797046382289054	27.342483809575047	28.53650663420736	23.32396317392854
43	22.355563924437956	27.19150767658155	25.78262126846852	24.670307130511972
44	22.479808203148643	24.20510561604315	27.988587683844546	25.326498496963666
45	21.86732930281979	23.103215220008206	26.824659210889113	28.204796266282894
46	25.337426613494372	26.758081762486636	25.72176006563595	22.182731558383043
47	19.504670508880356	25.905520548221578	32.00038332470292	22.589425618195147
48	21.196342947834214	26.63030686151218	26.882662290936725	25.29068789971688
49	21.22004855446237	24.126255052152334	31.619412369955413	23.034284023429883
50	21.459962743528873	24.324810523271843	29.051809359847745	25.16341737335154
51	20.488537246383633	23.34312940907471	27.22160202825843	28.94673131628323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	491.0
2	778.0
3	2279.5
4	3781.0
5	2545.0
6	1309.0
7	1290.5
8	1272.0
9	1240.5
10	1209.0
11	1236.0
12	1263.0
13	1204.0
14	1145.0
15	1219.5
16	1294.0
17	1356.5
18	1419.0
19	1360.5
20	1302.0
21	1523.5
22	1745.0
23	2014.5
24	2284.0
25	2772.0
26	3872.5
27	4485.0
28	5187.0
29	5889.0
30	7219.5
31	8550.0
32	9387.0
33	10224.0
34	12020.5
35	13817.0
36	14657.5
37	15498.0
38	17227.0
39	18956.0
40	21991.0
41	25026.0
42	27296.5
43	29567.0
44	34972.0
45	40377.0
46	60336.0
47	80295.0
48	68388.0
49	56481.0
50	56468.5
51	56456.0
52	51300.5
53	46145.0
54	41411.5
55	36678.0
56	33574.5
57	30471.0
58	27642.0
59	24813.0
60	22673.0
61	20533.0
62	18699.0
63	16865.0
64	14186.5
65	11508.0
66	9488.0
67	7468.0
68	6067.0
69	4666.0
70	3970.0
71	3274.0
72	2576.0
73	1878.0
74	1465.0
75	986.5
76	921.0
77	631.5
78	342.0
79	256.0
80	170.0
81	119.5
82	69.0
83	47.5
84	26.0
85	23.0
86	20.0
87	14.0
88	8.0
89	4.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	594796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.54592306789756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14538485287007	24.201835299782413
2	10.202399510616047	6.844978181821633
3	3.8469912454067807	3.871526170838739
4	2.1265190636159144	2.853441796419081
5	1.450249372558254	2.4324976940552947
6	1.095577201508076	2.2051289110039454
7	0.8933503189445542	2.097778275039704
8	0.7515027437355823	2.0167882581334227
9	0.6907734849269663	2.085537076443315
>10	6.681563732857527	39.87967152905351
>50	0.07134971900635582	1.6472355142578672
>100	0.03618450035318191	2.212441597300731
>500	0.0020385634001792623	0.5901667872561721
>1k	0.00458676765040334	2.533682441233045
>5k	0.0015289225501344467	4.527290467361151
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	9466	1.5914700166107372	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	8917	1.499169463143666	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	8098	1.361475194856724	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	3114	0.5235408442558457	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	0.3270028715727745	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1882	0.31641100478147133	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1679	0.2822816562317164	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	1455	0.2446216854181938	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	1376	0.23133982071163894	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	1285	0.21604045756864537	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	1043	0.1753542391004647	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	1041	0.17501798936105822	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	941	0.15820550239073566	No Hit
GAATATGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	925	0.15551550447548404	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	834	0.14021614133249047	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	752	0.12642990201682594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14610051177210337	0.0
2	0.0	0.0	0.0	0.969407998708801	0.0
3	0.0	0.0	0.0	1.2412659130189174	0.0
4	1.6812486970322599E-4	0.0	0.0	1.8712297997969052	0.0
5	1.6812486970322599E-4	0.0	0.0	4.3473728807860175	0.0
6	1.6812486970322599E-4	0.0	0.0	5.161433499889037	0.0
7	1.6812486970322599E-4	0.0	0.0	6.029630327036497	0.0
8	1.6812486970322599E-4	0.0	0.0	7.174056315106355	0.0
9	1.6812486970322599E-4	0.0	0.0	7.556708518550898	0.0
10	1.6812486970322599E-4	0.0	0.0	9.92390668397232	0.0
11	1.6812486970322599E-4	0.0	0.0	11.047653313068682	0.0
12	1.6812486970322599E-4	0.0	0.0	13.626016314837356	0.0
13	1.6812486970322599E-4	0.0	0.0	14.119294682546622	0.0
14	1.6812486970322599E-4	0.0	0.0	14.35113887786737	0.0
15	1.6812486970322599E-4	0.0	0.0	14.9103221945003	0.0
16	3.3624973940645197E-4	0.0	0.0	15.590051042710442	0.0
17	3.3624973940645197E-4	0.0	0.0	16.31080235912817	0.0
18	3.3624973940645197E-4	0.0	0.0	17.018608060578753	0.0
19	3.3624973940645197E-4	0.0	0.0	17.779541220855553	0.0
20	6.724994788129039E-4	0.0	0.0	18.29316269779891	0.0
21	6.724994788129039E-4	0.0	0.0	18.852009764692433	0.0
22	6.724994788129039E-4	0.0	0.0	19.517111749238396	0.0
23	6.724994788129039E-4	0.0	0.0	20.11345066207574	0.0
24	6.724994788129039E-4	0.0	0.0	20.590757167163197	0.0
25	6.724994788129039E-4	0.0	0.0	21.006193720199867	0.0
26	6.724994788129039E-4	0.0	0.0	21.419276525060692	0.0
27	6.724994788129039E-4	0.0	0.0	21.872709298650292	0.0
28	6.724994788129039E-4	0.0	0.0	22.28007585794121	0.0
29	8.406243485161299E-4	0.0	0.0	22.71585552021197	0.0
30	8.406243485161299E-4	0.0	0.0	23.213000759924412	0.0
31	8.406243485161299E-4	0.0	0.0	23.662398536641135	0.0
32	8.406243485161299E-4	0.0	0.0	24.114822561012517	0.0
33	8.406243485161299E-4	0.0	0.0	24.53732035857672	0.0
34	8.406243485161299E-4	0.0	0.0	24.97444501980511	0.0
35	8.406243485161299E-4	0.0	0.0	25.443849656016518	0.0
36	8.406243485161299E-4	0.0	0.0	25.86987807584449	0.0
37	8.406243485161299E-4	0.0	0.0	26.293720872366325	0.0
38	8.406243485161299E-4	0.0	0.0	26.734544280728183	0.0
39	8.406243485161299E-4	0.0	0.0	27.21016953711861	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAA	45	3.8380676E-10	45.000004	12
CCGTAGA	30	2.163728E-6	45.000004	41
ACCGTAG	30	2.163728E-6	45.000004	40
CAATCGA	90	0.0	45.000004	41
CGTCATA	25	3.8885268E-5	45.000004	38
GTACGAG	30	2.163728E-6	45.000004	1
TGCGAAG	40	6.8048394E-9	45.0	1
CGAATAC	40	6.8048394E-9	45.0	45
TATACGT	20	7.030539E-4	45.0	10
CGATGAA	1215	0.0	43.148148	19
CCGATGA	1195	0.0	42.552303	18
AGACCGG	85	0.0	42.35294	2
CGTTATT	145	0.0	41.89655	1
TAATACG	415	0.0	41.74699	4
TACGGCT	1085	0.0	41.474655	7
AATACGG	420	0.0	41.25	5
ACCCGTC	55	6.002665E-11	40.909092	39
CACAACG	160	0.0	40.78125	12
TTAACGG	50	1.0804797E-9	40.500004	2
TCAAGCG	100	0.0	40.500004	17
>>END_MODULE