SRR2935853.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935853.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532310
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	6459	1.2133906933929477	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	5931	1.1142003719637055	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	5672	1.0655445135353458	No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	2441	0.4585673761529936	TruSeq Adapter, Index 13 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1262	0.23707989705246943	RNA PCR Primer, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.23257124607841292	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	1225	0.23012906013413237	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT	997	0.18729687588059588	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	946	0.1777159925607259	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	913	0.17151659747139825	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	843	0.15836636546373353	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	762	0.14314966842629295	Illumina PCR Primer Index 4 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	712	0.1337566455636753	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	665	0.1249272040728147	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT	640	0.12023069264150588	No Hit
GAATATGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	640	0.12023069264150588	TruSeq Adapter, Index 13 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCCA	25	3.887974E-5	45.0	34
CGAATAT	25	3.887974E-5	45.0	14
TCCGTTC	20	7.02987E-4	45.0	20
CGTAAGG	85	0.0	45.0	2
TATAGCG	20	7.02987E-4	45.0	1
TTACGCG	20	7.02987E-4	45.0	1
CGATGTC	20	7.02987E-4	45.0	10
TCGACGT	20	7.02987E-4	45.0	26
TAAGACG	20	7.02987E-4	45.0	1
TGGTACG	20	7.02987E-4	45.0	1
CGACGGT	30	2.1632968E-6	44.999996	28
CCGATGA	605	0.0	42.02479	18
AGTACGG	65	0.0	41.53846	2
CGATGAA	620	0.0	41.370968	19
CACGCCG	50	1.0786607E-9	40.5	26
CTCGAAT	100	0.0	40.5	43
TACGGCT	845	0.0	40.473373	7
TAATACG	280	0.0	40.17857	4
GCGCGAC	45	1.9250365E-8	40.0	9
CGAATGC	90	0.0	40.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content