##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935853.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 532310 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97869474554301 33.0 31.0 34.0 30.0 34.0 2 32.08652664800586 33.0 31.0 34.0 30.0 34.0 3 32.062288891811164 33.0 31.0 34.0 30.0 34.0 4 35.696832672690725 37.0 35.0 37.0 33.0 37.0 5 32.03605981476959 37.0 35.0 37.0 0.0 37.0 6 33.79781330427758 37.0 35.0 37.0 17.0 37.0 7 35.43381488230543 37.0 35.0 37.0 32.0 37.0 8 35.63979635926434 37.0 35.0 37.0 33.0 37.0 9 37.578852548327106 39.0 37.0 39.0 35.0 39.0 10 37.158257406398526 39.0 37.0 39.0 34.0 39.0 11 37.15202231782232 39.0 37.0 39.0 34.0 39.0 12 37.13060622569555 39.0 37.0 39.0 34.0 39.0 13 37.04637523247732 39.0 37.0 39.0 33.0 39.0 14 38.294396122560165 40.0 38.0 41.0 33.0 41.0 15 38.36565347260055 40.0 38.0 41.0 34.0 41.0 16 38.38535252014803 40.0 38.0 41.0 34.0 41.0 17 38.308237681050514 40.0 37.0 41.0 34.0 41.0 18 38.25602750277094 40.0 37.0 41.0 34.0 41.0 19 38.1498562867502 40.0 37.0 41.0 34.0 41.0 20 38.099932370235386 40.0 37.0 41.0 33.0 41.0 21 38.15225338618474 40.0 37.0 41.0 34.0 41.0 22 38.2037741165862 40.0 37.0 41.0 34.0 41.0 23 38.17421239503297 40.0 37.0 41.0 34.0 41.0 24 38.16176663974 40.0 37.0 41.0 34.0 41.0 25 37.99255509007909 40.0 37.0 41.0 33.0 41.0 26 37.982664237004755 40.0 37.0 41.0 33.0 41.0 27 37.93346734045951 40.0 37.0 41.0 33.0 41.0 28 37.87848246322631 40.0 37.0 41.0 33.0 41.0 29 37.867575285078246 40.0 37.0 41.0 33.0 41.0 30 37.74015892994684 40.0 36.0 41.0 33.0 41.0 31 37.78415021322162 40.0 37.0 41.0 33.0 41.0 32 37.60566962859988 40.0 36.0 41.0 33.0 41.0 33 37.52881779414251 40.0 36.0 41.0 33.0 41.0 34 37.45917416542992 40.0 36.0 41.0 33.0 41.0 35 37.39090379665984 40.0 36.0 41.0 33.0 41.0 36 37.315385771448966 40.0 36.0 41.0 33.0 41.0 37 37.25241870338712 40.0 36.0 41.0 32.0 41.0 38 37.20297007382916 40.0 36.0 41.0 32.0 41.0 39 37.076539986098325 40.0 36.0 41.0 32.0 41.0 40 36.97397381225226 40.0 35.0 41.0 31.0 41.0 41 36.9241832766621 40.0 35.0 41.0 31.0 41.0 42 36.89983656140219 40.0 35.0 41.0 31.0 41.0 43 36.87314910484492 39.0 35.0 41.0 31.0 41.0 44 36.88682346752832 39.0 35.0 41.0 31.0 41.0 45 36.912101970656195 39.0 35.0 41.0 31.0 41.0 46 36.7624504518044 39.0 35.0 41.0 31.0 41.0 47 36.62567864590182 39.0 35.0 41.0 31.0 41.0 48 36.59894986004396 39.0 35.0 41.0 31.0 41.0 49 36.58476263831226 39.0 35.0 41.0 31.0 41.0 50 36.485758298735696 39.0 35.0 41.0 31.0 41.0 51 35.473444045762804 38.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 22.0 10 14.0 11 11.0 12 14.0 13 5.0 14 13.0 15 21.0 16 29.0 17 75.0 18 126.0 19 297.0 20 639.0 21 1237.0 22 1926.0 23 2467.0 24 3030.0 25 3547.0 26 3894.0 27 4335.0 28 4816.0 29 5451.0 30 7099.0 31 9459.0 32 12857.0 33 18214.0 34 29350.0 35 38434.0 36 39903.0 37 61266.0 38 103792.0 39 179825.0 40 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.094362307677855 19.481129417069003 22.53498901016325 10.889519265089891 2 30.561890627641787 28.609832616332586 23.93680374218031 16.891473013845314 3 30.580676673367023 29.113298641768893 24.55636753019857 15.749657154665513 4 26.950836918337057 23.770547237511977 30.642670624260298 18.635945219890665 5 25.882474497942926 35.34895079934625 21.16454697450734 17.60402772820349 6 23.688264357235443 38.170802727733836 23.749694726756964 14.39123818827375 7 77.01320659014485 5.175367736845072 13.368901579906446 4.442524093103643 8 77.21966523266518 9.261144821626496 8.568127594822565 4.951062350885762 9 72.40799534106065 6.039337979748643 9.755405684657436 11.79726099453326 10 40.19650203828596 24.871597377468017 19.198587289361463 15.73331329488456 11 30.693580808175685 24.368319212488963 24.59656966805057 20.341530311284778 12 25.168416899926733 21.455918543705735 29.38269053746877 23.992974018898764 13 24.679040408784356 22.64826886588642 33.16676372790292 19.50592699742631 14 21.331930641919183 28.214386353816383 28.742274238695497 21.711408765568933 15 18.95249009036088 25.160526760722135 34.23230824143826 21.654674907478725 16 21.441641148954556 25.515019443557325 28.16535477447352 24.877984633014595 17 21.40989273167891 26.40472656910447 28.99118934455486 23.194191354661758 18 21.587796584696886 25.264037872668183 29.785651218275067 23.362514324359864 19 21.734515601810976 26.777253855835887 27.106761097856513 24.381469444496627 20 23.551126223441226 26.911386222314064 30.194623433713435 19.34286412053127 21 22.59153500779621 29.886156562905075 28.30756514061355 19.214743288685167 22 21.275384644286223 24.051962202476002 29.811200240461382 24.86145291277639 23 21.280456876632037 27.69589149179989 28.885611767579043 22.13803986398903 24 21.881422479382316 26.48118577520618 27.738160094681668 23.89923165072984 25 19.909075538689862 30.5468617910616 26.73536097386861 22.80870169637993 26 19.582949784899775 25.776333339595347 29.638932201160976 25.001784674343895 27 22.65878905149255 26.20860025173301 28.211568446957603 22.921042249816836 28 18.272810956021868 26.695722417388364 30.523379233905057 24.508087392684715 29 20.255678082320454 24.556743251113073 29.45802258082696 25.729556085739514 30 20.055418834889444 28.01130919952659 30.398827750746744 21.53444421483722 31 21.873532340177714 25.661738460671412 25.71509083053108 26.749638368619788 32 23.09687963780504 28.655294846987655 26.137213277977118 22.110612237230185 33 21.755555973023238 26.770678739832054 26.03088425917229 25.44288102797242 34 22.430538595930944 24.77672784655558 26.68952302229904 26.103210535214444 35 20.30884259172287 25.464860701470947 29.569235971520353 24.657060735285828 36 20.53164509402416 26.254438203302588 26.399654336758655 26.814262365914598 37 19.63592643384494 26.555954237192616 30.565084255415076 23.243035073547368 38 18.98123274032049 28.089271289286316 25.85091394112453 27.07858202926866 39 22.60205519340234 27.540530893652193 26.538483214668148 23.31893069827732 40 21.064041629877327 25.926245984482726 30.088106554451354 22.921605831188593 41 20.86772745204862 24.72525408126843 27.054348030283105 27.35267043639984 42 19.998121395427475 23.572542315567997 31.363679059194833 25.065657229809695 43 21.435066032950726 24.47821757998159 26.849580131878042 27.237136255189647 44 20.69358080817569 24.789878078563245 28.660742800247974 25.855798313013096 45 20.696022994119968 24.155285453964794 27.35962127331818 27.789070278597055 46 24.049707876988975 25.94972854163927 26.94088031410268 23.059683267269072 47 18.946290695271554 24.736337848246322 32.58045124081832 23.736920215663805 48 20.46476677124232 25.032030207961526 28.55009299092634 25.953110029869812 49 19.937630328192217 23.27440776990851 32.40254738780034 24.385414514098926 50 20.80009768743777 23.215043865416767 30.38624110011084 25.598617347034626 51 20.539535233228758 22.764742349382878 28.067479476245044 28.628242941143316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 134.0 1 339.0 2 544.0 3 1429.0 4 2314.0 5 1543.0 6 772.0 7 777.0 8 782.0 9 804.0 10 826.0 11 832.0 12 838.0 13 847.5 14 857.0 15 828.0 16 799.0 17 853.5 18 908.0 19 995.5 20 1083.0 21 1151.0 22 1219.0 23 1432.0 24 1645.0 25 1976.5 26 3185.5 27 4063.0 28 5033.0 29 6003.0 30 6823.0 31 7643.0 32 8893.0 33 10143.0 34 11659.0 35 13175.0 36 14620.5 37 16066.0 38 18135.5 39 20205.0 40 22068.5 41 23932.0 42 25953.5 43 27975.0 44 32569.0 45 37163.0 46 52799.0 47 68435.0 48 61050.5 49 53666.0 50 54023.0 51 54380.0 52 47903.0 53 41426.0 54 37358.0 55 33290.0 56 29916.0 57 26542.0 58 23241.0 59 19940.0 60 17956.0 61 15972.0 62 14391.0 63 12810.0 64 10927.0 65 9044.0 66 7592.0 67 6140.0 68 4902.5 69 3665.0 70 3099.0 71 2533.0 72 1995.5 73 1458.0 74 1091.0 75 559.0 76 394.0 77 317.0 78 240.0 79 209.5 80 179.0 81 105.5 82 32.0 83 25.0 84 18.0 85 18.5 86 19.0 87 11.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 532310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.207305064741554 #Duplication Level Percentage of deduplicated Percentage of total 1 71.66093338859146 25.229883430362637 2 10.307559184020326 7.258027613293643 3 3.9239548000831785 4.144556211203562 4 2.203711063573007 3.1034691075904375 5 1.485724854932097 2.6154184104931617 6 1.1350944144028894 2.397816919510002 7 0.92621074931829 2.2826569083844332 8 0.8202268400206596 2.3102381263117064 9 0.7362970201885892 2.3330730427235853 >10 6.714034055819042 39.618532826191135 >50 0.05316261413175562 1.3193662324534208 >100 0.024411404448254237 1.4001554502194218 >500 0.004882280889650848 1.3594743547485806 >1k 0.002169902617622599 1.1776508670103607 >5k 0.0016274269632169493 3.449680499503914 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC 6459 1.2133906933929477 TruSeq Adapter, Index 13 (95% over 22bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC 5931 1.1142003719637055 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG 5672 1.0655445135353458 No Hit GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC 2441 0.4585673761529936 TruSeq Adapter, Index 13 (96% over 25bp) GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT 1262 0.23707989705246943 RNA PCR Primer, Index 13 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1238 0.23257124607841292 No Hit GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT 1225 0.23012906013413237 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCAAGTCT 997 0.18729687588059588 No Hit CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC 946 0.1777159925607259 TruSeq Adapter, Index 13 (96% over 25bp) GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC 913 0.17151659747139825 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA 843 0.15836636546373353 No Hit CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT 762 0.14314966842629295 Illumina PCR Primer Index 4 (95% over 23bp) GAATAATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC 712 0.1337566455636753 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG 665 0.1249272040728147 No Hit GAATGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT 640 0.12023069264150588 No Hit GAATATGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC 640 0.12023069264150588 TruSeq Adapter, Index 13 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.8786045725235296E-4 0.0 0.0 0.15348199357517237 0.0 2 1.8786045725235296E-4 0.0 0.0 0.9710507035374124 0.0 3 1.8786045725235296E-4 0.0 0.0 1.2584772031335125 0.0 4 1.8786045725235296E-4 0.0 0.0 1.8658300614303696 0.0 5 1.8786045725235296E-4 0.0 0.0 4.084649922037911 0.0 6 1.8786045725235296E-4 0.0 0.0 4.901279329713889 0.0 7 1.8786045725235296E-4 0.0 0.0 5.792677199376303 0.0 8 1.8786045725235296E-4 0.0 0.0 6.951212639251564 0.0 9 1.8786045725235296E-4 0.0 0.0 7.286355694989761 0.0 10 1.8786045725235296E-4 0.0 0.0 9.323890214348781 0.0 11 1.8786045725235296E-4 0.0 0.0 10.56282992992805 0.0 12 1.8786045725235296E-4 0.0 0.0 12.834438579023502 0.0 13 1.8786045725235296E-4 0.0 0.0 13.312731303187991 0.0 14 1.8786045725235296E-4 0.0 0.0 13.526704363998421 0.0 15 1.8786045725235296E-4 0.0 0.0 13.99522834438579 0.0 16 1.8786045725235296E-4 0.0 0.0 14.674907478724803 0.0 17 1.8786045725235296E-4 0.0 0.0 15.491161165486277 0.0 18 1.8786045725235296E-4 0.0 0.0 16.2914467133813 0.0 19 1.8786045725235296E-4 0.0 0.0 17.072570494636583 0.0 20 1.8786045725235296E-4 0.0 0.0 17.61905656478368 0.0 21 1.8786045725235296E-4 0.0 0.0 18.277319606995924 0.0 22 1.8786045725235296E-4 0.0 0.0 18.99626157690068 0.0 23 1.8786045725235296E-4 0.0 0.0 19.669741316150365 0.0 24 1.8786045725235296E-4 0.0 0.0 20.217354549040973 0.0 25 3.757209145047059E-4 0.0 0.0 20.715748342131466 0.0 26 3.757209145047059E-4 0.0 0.0 21.173939997369953 0.0 27 3.757209145047059E-4 0.0 0.0 21.6685765813154 0.0 28 3.757209145047059E-4 0.0 0.0 22.13559767804475 0.0 29 3.757209145047059E-4 0.0 0.0 22.61783547181154 0.0 30 3.757209145047059E-4 0.0 0.0 23.161503635099848 0.0 31 3.757209145047059E-4 0.0 0.0 23.671356916082733 0.0 32 3.757209145047059E-4 0.0 0.0 24.144013826529655 0.0 33 3.757209145047059E-4 0.0 0.0 24.649358456538483 0.0 34 3.757209145047059E-4 0.0 0.0 25.118446018297607 0.0 35 3.757209145047059E-4 0.0 0.0 25.62491781104995 0.0 36 3.757209145047059E-4 0.0 0.0 26.08217016400218 0.0 37 3.757209145047059E-4 0.0 0.0 26.554639213991848 0.0 38 3.757209145047059E-4 0.0 0.0 27.02823542672503 0.0 39 3.757209145047059E-4 0.0 0.0 27.504649546316994 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCCA 25 3.887974E-5 45.0 34 CGAATAT 25 3.887974E-5 45.0 14 TCCGTTC 20 7.02987E-4 45.0 20 CGTAAGG 85 0.0 45.0 2 TATAGCG 20 7.02987E-4 45.0 1 TTACGCG 20 7.02987E-4 45.0 1 CGATGTC 20 7.02987E-4 45.0 10 TCGACGT 20 7.02987E-4 45.0 26 TAAGACG 20 7.02987E-4 45.0 1 TGGTACG 20 7.02987E-4 45.0 1 CGACGGT 30 2.1632968E-6 44.999996 28 CCGATGA 605 0.0 42.02479 18 AGTACGG 65 0.0 41.53846 2 CGATGAA 620 0.0 41.370968 19 CACGCCG 50 1.0786607E-9 40.5 26 CTCGAAT 100 0.0 40.5 43 TACGGCT 845 0.0 40.473373 7 TAATACG 280 0.0 40.17857 4 GCGCGAC 45 1.9250365E-8 40.0 9 CGAATGC 90 0.0 40.0 45 >>END_MODULE