##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935852.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 749564 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907799734245508 33.0 31.0 34.0 30.0 34.0 2 32.03396374425666 33.0 31.0 34.0 30.0 34.0 3 31.92022962682306 33.0 31.0 34.0 30.0 34.0 4 35.64234941912899 37.0 35.0 37.0 33.0 37.0 5 31.937951929388284 37.0 35.0 37.0 0.0 37.0 6 33.72893175232535 37.0 35.0 37.0 17.0 37.0 7 35.39205858338981 37.0 35.0 37.0 32.0 37.0 8 35.6010614170371 37.0 35.0 37.0 33.0 37.0 9 37.564875047360864 39.0 37.0 39.0 35.0 39.0 10 37.225391027317215 39.0 37.0 39.0 34.0 39.0 11 37.207437923913105 39.0 37.0 39.0 34.0 39.0 12 37.21380962799707 39.0 37.0 39.0 34.0 39.0 13 37.15853216002903 39.0 37.0 39.0 33.0 39.0 14 38.35195260177917 40.0 38.0 41.0 34.0 41.0 15 38.439303381699226 40.0 38.0 41.0 34.0 41.0 16 38.42941763478502 40.0 38.0 41.0 34.0 41.0 17 38.38340021665928 40.0 38.0 41.0 34.0 41.0 18 38.20984465636023 40.0 38.0 41.0 34.0 41.0 19 38.00526172548308 40.0 37.0 41.0 34.0 41.0 20 37.919828060045575 40.0 36.0 41.0 33.0 41.0 21 37.90871493294769 40.0 36.0 41.0 33.0 41.0 22 37.990913384314084 40.0 36.0 41.0 34.0 41.0 23 37.98233906644396 40.0 36.0 41.0 34.0 41.0 24 37.933612073151856 40.0 36.0 41.0 34.0 41.0 25 37.716971466078945 40.0 35.0 41.0 33.0 41.0 26 37.715578656392246 40.0 35.0 41.0 33.0 41.0 27 37.70670816634737 40.0 35.0 41.0 33.0 41.0 28 37.62184016308147 40.0 35.0 41.0 33.0 41.0 29 37.57345203344878 40.0 35.0 41.0 33.0 41.0 30 37.36844218772513 40.0 35.0 41.0 33.0 41.0 31 37.25848359846524 40.0 35.0 41.0 32.0 41.0 32 37.031102080676234 40.0 35.0 41.0 32.0 41.0 33 36.76419491864604 40.0 35.0 41.0 31.0 41.0 34 36.5363971588817 40.0 35.0 41.0 30.0 41.0 35 36.39654385749582 40.0 35.0 41.0 30.0 41.0 36 36.25694136858227 40.0 35.0 41.0 29.0 41.0 37 36.21714490023534 40.0 35.0 41.0 29.0 41.0 38 36.042850243608285 39.0 35.0 41.0 28.0 41.0 39 35.92510179250871 39.0 35.0 41.0 27.0 41.0 40 35.8375655714522 39.0 35.0 41.0 26.0 41.0 41 35.721612830925714 39.0 35.0 41.0 26.0 41.0 42 35.74970649604303 39.0 35.0 41.0 26.0 41.0 43 35.68001131324343 39.0 35.0 41.0 26.0 41.0 44 35.660642720301404 39.0 35.0 41.0 26.0 41.0 45 35.634615589862904 39.0 35.0 41.0 26.0 41.0 46 35.511820204812395 39.0 35.0 41.0 26.0 41.0 47 35.39390232188312 39.0 35.0 41.0 25.0 41.0 48 35.38876200030951 39.0 35.0 41.0 25.0 41.0 49 35.37146527848189 39.0 35.0 41.0 25.0 41.0 50 35.25633434903491 38.0 35.0 40.0 24.0 41.0 51 34.319545762603326 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 18.0 10 29.0 11 23.0 12 14.0 13 11.0 14 10.0 15 22.0 16 52.0 17 109.0 18 255.0 19 536.0 20 935.0 21 1696.0 22 2791.0 23 4127.0 24 5933.0 25 8816.0 26 11237.0 27 12231.0 28 11640.0 29 11333.0 30 12391.0 31 15018.0 32 19347.0 33 26806.0 34 41500.0 35 51178.0 36 58038.0 37 87719.0 38 140504.0 39 225077.0 40 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93807600151555 18.225261618754367 20.954981829436846 16.881680550293236 2 35.9298205356714 26.17855179811197 21.909803565806254 15.981824100410371 3 28.192789408242657 26.74074528659327 30.113906217481095 14.952559087682973 4 25.583939463474763 22.255337769690115 34.47831005758014 17.68241270925498 5 22.876498871343877 33.47599404453789 26.17895203078056 17.468555053337674 6 23.051667369297352 33.059885480092426 29.379079037947392 14.509368112662829 7 72.79365070894546 4.584665218713813 17.88146175643441 4.740222315906314 8 72.29829607611892 6.9847271213665545 15.22605141122039 5.490925391294139 9 66.76814788330283 6.059656013362435 16.582840157745036 10.589355945589702 10 34.65107715952207 26.77476506342354 23.665224050247875 14.908933726806517 11 26.698454034612123 22.205842329674315 31.328745777545347 19.766957858168215 12 22.95908021196322 19.930653019622074 35.72530164202123 21.384965126393478 13 22.823001104642167 20.78661728684942 38.623119573512064 17.767262034996346 14 19.093499687818518 26.411620622121664 34.75860633648361 19.736273353576212 15 17.657464872912787 24.060653926816123 38.42166913032109 19.86021206995 16 21.113740787978077 24.963045183600066 32.64804606411194 21.27516796430992 17 21.089193184304474 25.490952073472045 31.833305761749497 21.58654898047398 18 21.592819292281913 24.18125737095165 32.710348949522654 21.515574387243785 19 20.300734827179532 26.293151752218623 30.473048332097058 22.933065088504783 20 21.828956566750804 26.701789306850383 31.949773468309576 19.519480658089236 21 21.09666419411818 27.293199780138856 33.44344178749246 18.166694238250503 22 20.58623413077469 22.353528184384523 33.68598278465881 23.374254900181974 23 19.89356479233261 25.556323409341964 33.68971828956567 20.86039350875976 24 21.133218777849525 24.687418285830162 32.383358859283526 21.796004077036784 25 20.460961305505602 28.190921655789232 29.81199737447369 21.536119664231475 26 19.93305441563362 24.413525729624155 31.21841497190367 24.435004882838555 27 22.50254814799003 23.697376074624714 30.256389047499617 23.54368672988564 28 19.137658692253094 24.16698240577189 32.202587103969776 24.492771798005244 29 20.519662096898998 22.422768436050823 31.181326744614204 25.876242722435975 30 20.656408258667707 22.951609202149516 32.096525446793066 24.295457092389707 31 21.610696351478992 22.178226275541515 32.254083707328526 23.956993665650966 32 24.04237663495045 23.101296220202677 30.654620552747996 22.201706592098873 33 21.759983136863564 21.6225699206472 32.97196770389186 23.64547923859737 34 19.615269676772094 22.10565075163695 34.62025924404053 23.658820327550416 35 20.128634779685257 22.076433766829783 31.303130886755497 26.491800566729456 36 19.93932472744155 23.49259035919548 33.223180408877695 23.344904504485275 37 19.939724960110144 23.044329770373174 33.333911447187965 23.682033822328712 38 20.330885688213414 23.439626236051893 31.97151944330304 24.257968632431652 39 22.694659828913874 22.33778569941993 32.148955926378534 22.81859854528766 40 21.451403749379637 22.08817392510846 33.54937003377964 22.911052291732258 41 19.047606341820046 24.431936432379356 32.04903117012023 24.471426055680368 42 20.56248699243827 22.97682386027077 32.699809489249745 23.76087965804121 43 21.918608684515263 22.568853360086663 31.613578026692853 23.89895992870522 44 21.82775586874503 22.48186946011281 30.653419854742225 25.03695481639993 45 21.184715381208274 21.382030086823807 30.086023341569234 27.347231190398684 46 23.491656482968764 22.581527394592058 29.986098585310927 23.94071753712825 47 19.665565582125076 22.637293146415782 34.38598972202507 23.31115154943407 48 19.94159271256357 22.86582600018144 31.855318558522022 25.337262728732966 49 20.65387345176663 21.181113287190954 34.592376368128676 23.57263689291375 50 19.911975495087813 21.23341035588689 34.335960638451155 24.518653510574147 51 19.34151053145562 20.764070846518777 31.797685054244866 28.096733567780735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 176.0 1 461.5 2 747.0 3 8063.0 4 15379.0 5 10192.0 6 5005.0 7 4863.0 8 4721.0 9 4754.5 10 4788.0 11 4652.5 12 4517.0 13 4326.0 14 4135.0 15 3929.5 16 3724.0 17 3549.5 18 3375.0 19 3280.0 20 3185.0 21 3230.5 22 3276.0 23 3431.0 24 3586.0 25 4097.5 26 5338.5 27 6068.0 28 6628.5 29 7189.0 30 8704.0 31 10219.0 32 12036.0 33 13853.0 34 15724.5 35 17596.0 36 18967.0 37 20338.0 38 22492.5 39 24647.0 40 26417.0 41 28187.0 42 31470.5 43 34754.0 44 40656.0 45 46558.0 46 68840.5 47 91123.0 48 83250.5 49 75378.0 50 73380.5 51 71383.0 52 62915.0 53 54447.0 54 48408.0 55 42369.0 56 38420.0 57 34471.0 58 31512.0 59 28553.0 60 25746.5 61 22940.0 62 20800.0 63 18660.0 64 16176.5 65 13693.0 66 11152.0 67 8611.0 68 7411.0 69 6211.0 70 5350.5 71 4490.0 72 3681.5 73 2873.0 74 2354.5 75 1397.5 76 959.0 77 700.0 78 441.0 79 357.5 80 274.0 81 205.5 82 137.0 83 96.0 84 55.0 85 36.0 86 17.0 87 11.0 88 5.0 89 3.5 90 2.0 91 2.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 749564.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.349001661420665 #Duplication Level Percentage of deduplicated Percentage of total 1 73.68262868991951 28.99337878737485 2 9.750277691227001 7.673273861428083 3 4.10817006127437 4.8495717169945145 4 2.3465678385042064 3.693404071037533 5 1.55373086584577 3.056887921078288 6 1.2599350184413094 2.97463110803575 7 1.00380623062355 2.764914112558534 8 0.8517463233011305 2.68122939925481 9 0.7305715450510828 2.5872534846001547 >10 4.63269401657473 29.74388289920545 >50 0.04799159526055359 1.290061500063425 >100 0.023652995208560016 1.7011974902519533 >500 0.002056782192048458 0.5073107961336167 >1k 0.005484752512129221 4.642952649219682 >5k 3.427970320080763E-4 1.1689325603014948 >10k+ 3.427970320080763E-4 1.6711176424619456 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC 12389 1.6528275103927084 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8666 1.156138768670854 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC 4623 0.6167585422992566 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG 4517 0.6026169880090293 No Hit GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT 3955 0.527640068092918 No Hit GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC 3126 0.41704244067217744 No Hit CGTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT 2505 0.3341942782737698 No Hit CGTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC 2116 0.2822974422464259 No Hit GAATGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT 2037 0.27175798197352063 No Hit CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG 1810 0.24147371005010912 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT 1405 0.18744229979027807 No Hit GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT 1336 0.17823694841267723 No Hit CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC 1283 0.17116617126756356 No Hit GAATATGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC 1212 0.1616939981109018 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC 1198 0.15982624565747555 No Hit CGTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT 1183 0.15782508231451883 No Hit CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT 1093 0.1458181022567786 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1022 0.13634592910011686 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.6682177906089407E-4 0.0 0.0 0.1503540725008138 0.0 2 2.6682177906089407E-4 0.0 0.0 0.8462252722916256 0.0 3 2.6682177906089407E-4 0.0 0.0 1.286881440410692 0.0 4 2.6682177906089407E-4 0.0 0.0 2.2754561318313047 0.0 5 2.6682177906089407E-4 0.0 0.0 5.084689232673928 0.0 6 2.6682177906089407E-4 0.0 0.0 6.803555133384207 0.0 7 2.6682177906089407E-4 0.0 0.0 8.033203302186338 0.0 8 2.6682177906089407E-4 0.0 0.0 9.548350774583625 0.0 9 2.6682177906089407E-4 0.0 0.0 10.262899498908698 0.0 10 2.6682177906089407E-4 0.0 0.0 11.987368656979257 0.0 11 2.6682177906089407E-4 0.0 0.0 13.18793325186375 0.0 12 4.002326685913411E-4 0.0 0.0 14.844629678052842 0.0 13 4.002326685913411E-4 0.0 0.0 15.398284869604197 0.0 14 4.002326685913411E-4 0.0 0.0 15.722339920273653 0.0 15 4.002326685913411E-4 0.0 0.0 16.13044383134729 0.0 16 4.002326685913411E-4 0.0 0.0 16.69423825050296 0.0 17 4.002326685913411E-4 0.0 0.0 17.340880832057035 0.0 18 4.002326685913411E-4 0.0 0.0 18.01140396283706 0.0 19 4.002326685913411E-4 0.0 0.0 18.560923416812972 0.0 20 4.002326685913411E-4 0.0 0.0 19.017855713454754 0.0 21 4.002326685913411E-4 0.0 0.0 19.54189368753035 0.0 22 4.002326685913411E-4 0.0 0.0 20.140508348853466 0.0 23 4.002326685913411E-4 0.0 0.0 20.683357258352856 0.0 24 4.002326685913411E-4 0.0 0.0 21.139622500546984 0.0 25 4.002326685913411E-4 0.0 0.0 21.571340139067512 0.0 26 4.002326685913411E-4 0.0 0.0 21.9770426541296 0.0 27 4.002326685913411E-4 0.0 0.0 22.41516401534759 0.0 28 4.002326685913411E-4 0.0 0.0 22.822734282863106 0.0 29 4.002326685913411E-4 0.0 0.0 23.268059832115735 0.0 30 5.336435581217881E-4 0.0 0.0 23.81464424652198 0.0 31 5.336435581217881E-4 0.0 0.0 24.278513909419342 0.0 32 5.336435581217881E-4 0.0 0.0 24.76759823043796 0.0 33 5.336435581217881E-4 0.0 0.0 25.222796185515847 0.0 34 5.336435581217881E-4 0.0 0.0 25.67412522479735 0.0 35 5.336435581217881E-4 0.0 0.0 26.162809313147378 0.0 36 5.336435581217881E-4 0.0 0.0 26.61707339199855 0.0 37 5.336435581217881E-4 0.0 0.0 27.05866343634433 0.0 38 5.336435581217881E-4 0.0 0.0 27.53627442086333 0.0 39 5.336435581217881E-4 0.0 0.0 28.146629240465124 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCATA 20 7.0317136E-4 45.000004 38 CGATTGA 20 7.0317136E-4 45.000004 10 CCGGTTA 20 7.0317136E-4 45.000004 30 CGTTTTT 4785 0.0 43.448277 1 CCGATGA 615 0.0 41.341465 18 CGTTATT 620 0.0 41.008068 1 CGAGACA 110 0.0 40.909092 22 ACGGGTA 50 1.0804797E-9 40.500004 5 CGGGATA 145 0.0 40.34483 6 TAATACG 185 0.0 40.135136 4 CGATGAA 640 0.0 40.07813 19 GGTACGC 40 3.45608E-7 39.375004 8 CCAACGG 40 3.45608E-7 39.375004 2 TACGGCT 600 0.0 38.625004 7 GCTTACG 35 6.24538E-6 38.57143 1 ACGGGAG 450 0.0 38.5 5 CACAACG 170 0.0 38.382355 12 CGGGTAT 65 9.094947E-12 38.076927 6 GCGATTT 65 9.094947E-12 38.076927 9 CGAACAC 30 1.1395429E-4 37.500004 34 >>END_MODULE