SRR2935851.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935851.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489753
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.433892186469506	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1302	0.2658482949568456	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1095	0.22358209138075724	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1026	0.20949335685539447	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	801	0.16355183122921146	TruSeq Adapter, Index 3 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	654	0.13353670115343858	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	633	0.12924882542832816	TruSeq Adapter, Index 3 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGACG	20	7.029316E-4	45.000004	1
TATAGCG	40	6.8012014E-9	45.000004	1
TTGCACG	20	7.029316E-4	45.000004	1
GTACGAG	35	1.21001E-7	45.000004	1
TAGGTCC	25	3.8875165E-5	45.0	23
CGCTATC	25	3.8875165E-5	45.0	1
AGTAGCG	25	3.8875165E-5	45.0	1
CTACGAA	50	1.0786607E-9	40.5	11
CGAATAT	50	1.0786607E-9	40.5	14
CGTATGG	90	0.0	40.0	2
TACGGGA	215	0.0	39.76744	4
ACGTTAG	40	3.4528057E-7	39.375004	1
AACGAGC	195	0.0	39.23077	15
TAACGGG	150	0.0	39.000004	3
TGTTGCG	35	6.240947E-6	38.57143	1
CCGCGCA	35	6.240947E-6	38.57143	29
GCTAACG	35	6.240947E-6	38.57143	1
CGTTTTT	1000	0.0	38.250004	1
CAACGAG	190	0.0	37.894737	14
ACGGGAC	190	0.0	37.894737	5