##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935851.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489753
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11164607465396	33.0	31.0	34.0	30.0	34.0
2	32.22205887457555	33.0	31.0	34.0	30.0	34.0
3	32.16411538061023	33.0	31.0	34.0	30.0	34.0
4	35.80114057494288	37.0	35.0	37.0	35.0	37.0
5	32.11418204686852	37.0	35.0	37.0	0.0	37.0
6	33.903604470008354	37.0	35.0	37.0	17.0	37.0
7	35.56711035971194	37.0	35.0	37.0	32.0	37.0
8	35.817073096030036	37.0	35.0	37.0	35.0	37.0
9	37.76354815590716	39.0	38.0	39.0	35.0	39.0
10	37.419521677253634	39.0	37.0	39.0	34.0	39.0
11	37.3675893766858	39.0	37.0	39.0	35.0	39.0
12	37.23970654595276	39.0	37.0	39.0	34.0	39.0
13	37.15220529532234	39.0	37.0	39.0	34.0	39.0
14	38.17700963546931	40.0	38.0	41.0	33.0	41.0
15	38.366550077283854	40.0	38.0	41.0	34.0	41.0
16	38.369504627843014	40.0	38.0	41.0	34.0	41.0
17	38.399983256866214	40.0	37.0	41.0	34.0	41.0
18	38.322272655808135	40.0	37.0	41.0	34.0	41.0
19	38.20473381480052	40.0	37.0	41.0	34.0	41.0
20	38.28663836668688	40.0	37.0	41.0	34.0	41.0
21	38.18609176462421	40.0	37.0	41.0	34.0	41.0
22	38.290189136156386	40.0	37.0	41.0	34.0	41.0
23	38.31659428324073	40.0	37.0	41.0	34.0	41.0
24	38.29045253423664	40.0	37.0	41.0	34.0	41.0
25	38.007944821164955	40.0	36.0	41.0	34.0	41.0
26	38.05522783933942	40.0	36.0	41.0	34.0	41.0
27	38.02860217293207	40.0	36.0	41.0	34.0	41.0
28	37.989794855774235	40.0	36.0	41.0	34.0	41.0
29	37.95690684896264	40.0	36.0	41.0	34.0	41.0
30	37.785773645082315	40.0	36.0	41.0	33.0	41.0
31	37.71928502735052	40.0	36.0	41.0	33.0	41.0
32	37.672342997388476	40.0	36.0	41.0	33.0	41.0
33	37.595116313733655	40.0	36.0	41.0	33.0	41.0
34	37.44948576118983	40.0	35.0	41.0	33.0	41.0
35	37.429163272098386	40.0	35.0	41.0	33.0	41.0
36	37.34483096581338	40.0	35.0	41.0	33.0	41.0
37	37.28384103823764	40.0	35.0	41.0	33.0	41.0
38	37.15241764726301	40.0	35.0	41.0	32.0	41.0
39	37.07536247863719	40.0	35.0	41.0	32.0	41.0
40	37.00748744775428	39.0	35.0	41.0	32.0	41.0
41	36.90583008169424	39.0	35.0	41.0	32.0	41.0
42	36.91164934160689	39.0	35.0	41.0	32.0	41.0
43	36.83905356373519	39.0	35.0	41.0	32.0	41.0
44	36.775703262665054	39.0	35.0	41.0	32.0	41.0
45	36.7506763613495	39.0	35.0	41.0	32.0	41.0
46	36.67664516603267	39.0	35.0	41.0	32.0	41.0
47	36.608512862606254	39.0	35.0	41.0	31.0	41.0
48	36.55976175745733	39.0	35.0	41.0	31.0	41.0
49	36.52504017331185	39.0	35.0	41.0	31.0	41.0
50	36.413714668414485	39.0	35.0	41.0	31.0	41.0
51	35.36279308141042	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	30.0
11	25.0
12	17.0
13	12.0
14	24.0
15	29.0
16	44.0
17	52.0
18	112.0
19	177.0
20	379.0
21	552.0
22	834.0
23	1226.0
24	1885.0
25	2712.0
26	3665.0
27	3881.0
28	4538.0
29	5253.0
30	6480.0
31	8660.0
32	11673.0
33	18123.0
34	32353.0
35	36559.0
36	37395.0
37	58494.0
38	94470.0
39	159950.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.295593901415614	20.33412761126527	23.574332367540375	11.795946119778746
2	34.836131682705364	23.343603816617765	24.249162332849416	17.57110216782746
3	33.398672392001686	23.054070112893644	26.660990335944856	16.88626715915982
4	29.67904229274757	24.95604927381762	26.07171370057968	19.29319473285513
5	22.695317843892738	36.5061571853567	23.082860135619384	17.71566483513118
6	26.727554501963237	32.99806228854137	25.192290807815372	15.08209240168003
7	80.36030407164428	5.600169881552538	9.455174342985137	4.584351703818047
8	80.84728424328182	4.949433694127448	9.443535823159838	4.759746239430897
9	74.47080467092596	7.404344639032328	11.715089034676664	6.409761655365051
10	39.65080356832934	27.75970744436481	18.11688749226651	14.472601495039337
11	31.59449763452189	23.936147404916355	25.23598630329983	19.233368657261927
12	29.236165985711164	21.47633603061135	29.34438380163062	19.943114182046866
13	27.067725976155327	22.23141052734746	31.3982762739585	19.30258722253871
14	21.885930254638563	25.046094664044936	32.10475484581003	20.96322023550647
15	20.398854116258605	24.52501567116485	34.408569217544354	20.66756099503219
16	25.938993737659594	24.69795999207764	30.076385443274468	19.286660826988296
17	25.66293621478582	23.69214685770174	28.279357145336526	22.365559782175914
18	25.97758461918559	23.20353320959749	30.54233460540313	20.27654756581379
19	24.93828521724216	25.32011034133533	27.64332224611182	22.0982821953107
20	24.460901719846532	27.740718280439324	27.366243800446348	20.432136199267795
21	24.950740475300815	25.77176658438029	30.47434114747638	18.803151792842517
22	24.008224554009878	23.085310350319446	30.222785771603238	22.683679324067437
23	22.49480860760424	25.279273429667608	30.311197685363844	21.914720277364307
24	22.98750594687526	25.053036939028452	28.97297209001272	22.98648502408357
25	23.77770018764561	25.707040079386957	27.542250889734216	22.973008843233224
26	22.41272641515213	25.03792728171139	28.33836648269638	24.210979820440098
27	22.674286834383864	24.298574995967353	30.339375154414572	22.68776301523421
28	20.196915588061735	25.36809371254489	29.833201634293204	24.601789065100164
29	23.06836303197734	25.468552515247485	29.150612655767294	22.31247179700788
30	25.201887482057284	23.40955542896113	28.81799601023373	22.570561078747858
31	24.332878001768236	25.053649492703467	28.793698047791437	21.81977445773686
32	26.542359107550134	24.330631971626516	28.079460462723045	21.04754845810031
33	25.861403605490928	23.97575920923404	27.72499606944725	22.437841115827776
34	21.8342715613789	25.005870306052238	30.969692886005802	22.190165246563062
35	22.94830251167425	25.34583759568599	29.617991109804333	22.08786878283543
36	25.53246228200746	25.333790706744015	27.34643789828751	21.787309112961022
37	23.627624537266744	26.755119417338946	27.786455621507166	21.83080042388714
38	23.56065200213169	25.5935134649507	28.628921109212197	22.21691342370542
39	23.965754165875452	24.197299455031413	27.44832599289846	24.388620386194674
40	24.580553871032947	23.422010687019785	29.6194204017127	22.378015040234565
41	21.94391866920672	24.672232737726976	28.40207206489802	24.981776528168282
42	23.514302107388826	23.668461448934465	30.034323424256716	22.782913019419993
43	23.632933335783548	23.213129883839404	29.707832315473308	23.446104464903737
44	23.540846099972843	23.61394417185806	28.577874969627548	24.26733475854155
45	23.005678372567395	23.03569350264317	28.08783202961493	25.87079609517451
46	23.364839010684978	23.924100515974377	28.46557346254132	24.245487010799323
47	21.412834632968046	25.09857009553795	30.55560660169514	22.932988669798856
48	21.73973411086813	25.40505111760418	29.541217715869017	23.31399705565867
49	24.085406317061867	23.35748836658479	29.723350341907047	22.8337549744463
50	22.12972661729484	24.16156715732216	29.410131229415644	24.298574995967353
51	21.813036367311685	23.47326101116277	28.429432795715393	26.284269825810153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197.0
1	445.0
2	693.0
3	2210.0
4	3727.0
5	2403.5
6	1080.0
7	1055.5
8	1031.0
9	1055.5
10	1080.0
11	1061.0
12	1042.0
13	1060.0
14	1078.0
15	1038.0
16	998.0
17	1051.0
18	1104.0
19	1071.0
20	1038.0
21	1094.0
22	1150.0
23	1579.5
24	2009.0
25	2368.5
26	3396.5
27	4065.0
28	4619.0
29	5173.0
30	6191.5
31	7210.0
32	8171.5
33	9133.0
34	10329.5
35	11526.0
36	12369.5
37	13213.0
38	14666.0
39	16119.0
40	18354.5
41	20590.0
42	22465.0
43	24340.0
44	26492.0
45	28644.0
46	34072.5
47	39501.0
48	43376.0
49	47251.0
50	47357.5
51	47464.0
52	43624.0
53	39784.0
54	35997.0
55	32210.0
56	29586.5
57	26963.0
58	26049.5
59	25136.0
60	22295.5
61	19455.0
62	17734.5
63	16014.0
64	14393.5
65	12773.0
66	10724.0
67	8675.0
68	7509.5
69	6344.0
70	5096.0
71	3848.0
72	3070.0
73	2292.0
74	1910.0
75	1181.0
76	834.0
77	643.0
78	452.0
79	308.5
80	165.0
81	110.5
82	56.0
83	40.0
84	24.0
85	13.5
86	3.0
87	5.5
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.30848624203204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22381975916643	25.860290798306647
2	10.134370695536678	7.359273179410923
3	3.984627529029373	4.340273814521554
4	2.356434215570679	3.422342371852062
5	1.540228826411517	2.796168857667186
6	1.2254422747873754	2.6696372384713127
7	1.0312803353516256	2.621095950745488
8	0.8641969503915927	2.5102146466959354
9	0.7783686293520891	2.54352480030537
>10	6.772500324919567	40.997909182863104
>50	0.0520933665352228	1.3221312511786871
>100	0.03262991090667796	1.9699984277113003
>500	0.001717363731930419	0.4339899469361886
>1k	0.0022898183092405584	1.1531495333342787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.433892186469506	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1302	0.2658482949568456	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1095	0.22358209138075724	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1026	0.20949335685539447	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	801	0.16355183122921146	TruSeq Adapter, Index 3 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	654	0.13353670115343858	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	633	0.12924882542832816	TruSeq Adapter, Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.083691166771822E-4	0.0	0.0	0.1674313378376447	0.0
2	4.083691166771822E-4	0.0	0.0	0.7297556115021245	0.0
3	4.083691166771822E-4	0.0	0.0	1.0642099180607367	0.0
4	4.083691166771822E-4	0.0	0.0	1.4229621870616413	0.0
5	4.083691166771822E-4	0.0	0.0	2.47818798455548	0.0
6	4.083691166771822E-4	0.0	0.0	3.316569781093735	0.0
7	4.083691166771822E-4	0.0	0.0	4.055717882279435	0.0
8	4.083691166771822E-4	0.0	0.0	5.1703613862498035	0.0
9	4.083691166771822E-4	0.0	0.0	5.67224703064606	0.0
10	4.083691166771822E-4	0.0	0.0	6.64620737392114	0.0
11	4.083691166771822E-4	0.0	0.0	7.887036934944758	0.0
12	4.083691166771822E-4	0.0	0.0	8.82730682609397	0.0
13	4.083691166771822E-4	0.0	0.0	9.256706952280027	0.0
14	4.083691166771822E-4	0.0	0.0	9.491927563486083	0.0
15	4.083691166771822E-4	0.0	0.0	9.820664702411216	0.0
16	4.083691166771822E-4	0.0	0.0	10.410962260568082	0.0
17	4.083691166771822E-4	0.0	0.0	11.074357890610164	0.0
18	6.125536750157733E-4	0.0	0.0	11.810239038862447	0.0
19	6.125536750157733E-4	0.0	0.0	12.26781663409923	0.0
20	6.125536750157733E-4	0.0	0.0	12.743770839586485	0.0
21	6.125536750157733E-4	0.0	0.0	13.292618932400618	0.0
22	6.125536750157733E-4	0.0	0.0	13.88965458098266	0.0
23	6.125536750157733E-4	0.0	0.0	14.48362746118962	0.0
24	6.125536750157733E-4	0.0	0.0	14.96039840491023	0.0
25	6.125536750157733E-4	0.0	0.0	15.4200178457304	0.0
26	6.125536750157733E-4	0.0	0.0	15.859218830716708	0.0
27	6.125536750157733E-4	0.0	0.0	16.327618207545438	0.0
28	6.125536750157733E-4	0.0	0.0	16.759264363873218	0.0
29	6.125536750157733E-4	0.0	0.0	17.232564170102073	0.0
30	6.125536750157733E-4	0.0	0.0	17.901676967777636	0.0
31	6.125536750157733E-4	0.0	0.0	18.40417516584891	0.0
32	6.125536750157733E-4	0.0	0.0	18.869307589744217	0.0
33	6.125536750157733E-4	0.0	0.0	19.362413298131916	0.0
34	6.125536750157733E-4	0.0	0.0	19.881042076311935	0.0
35	6.125536750157733E-4	0.0	0.0	20.432544568384472	0.0
36	6.125536750157733E-4	0.0	0.0	20.918503817230317	0.0
37	6.125536750157733E-4	0.0	0.0	21.41222207929303	0.0
38	6.125536750157733E-4	0.0	0.0	21.973729614724157	0.0
39	6.125536750157733E-4	0.0	0.0	22.8141532568458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGACG	20	7.029316E-4	45.000004	1
TATAGCG	40	6.8012014E-9	45.000004	1
TTGCACG	20	7.029316E-4	45.000004	1
GTACGAG	35	1.21001E-7	45.000004	1
TAGGTCC	25	3.8875165E-5	45.0	23
CGCTATC	25	3.8875165E-5	45.0	1
AGTAGCG	25	3.8875165E-5	45.0	1
CTACGAA	50	1.0786607E-9	40.5	11
CGAATAT	50	1.0786607E-9	40.5	14
CGTATGG	90	0.0	40.0	2
TACGGGA	215	0.0	39.76744	4
ACGTTAG	40	3.4528057E-7	39.375004	1
AACGAGC	195	0.0	39.23077	15
TAACGGG	150	0.0	39.000004	3
TGTTGCG	35	6.240947E-6	38.57143	1
CCGCGCA	35	6.240947E-6	38.57143	29
GCTAACG	35	6.240947E-6	38.57143	1
CGTTTTT	1000	0.0	38.250004	1
CAACGAG	190	0.0	37.894737	14
ACGGGAC	190	0.0	37.894737	5
>>END_MODULE