FastQCFastQC Report
Sat 14 Jan 2017
SRR2935848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644706
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC198953.0859027215505983No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG90331.4011037589226716No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC86031.3344066908016987No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84401.3091238487000276No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT62390.967727925597094No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC38220.5928283589729272TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT28590.44345794827409707No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT22480.3486860677580168No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC19610.3041696525237861No Hit
GAATATGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC16460.25531017238865467No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT14720.22832112621877257No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT14670.22754557891504035No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG12560.19481748269753965No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA12070.18721711912096367No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC11960.18551091505275272No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT9890.15340325667823781No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.1516970526100269No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG9510.1475090971698728No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC8640.13401457408493173No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC7270.11276457796266824TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT6780.10516421438609228No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC253.8888928E-545.0000049
GTATACG253.8888928E-545.0000041
GGCGCGA253.8888928E-545.0000048
GATTCGC253.8888928E-545.00000413
CGGGTGA207.030979E-445.06
CGCGCGA207.030979E-445.041
CCGACGT207.030979E-445.044
ATCGACG207.030979E-445.01
CGGACGG207.030979E-445.02
CGTTTTT42350.043.778041
ACGGCTG10700.043.3177578
CCGATGA18600.043.18548618
CGATGAA19250.042.6623419
CATCGAA1850.042.56756642
GCGAACC900.042.533
TAATACG4050.042.2222254
GATGAAT19950.041.95488720
CGTTATT5400.041.6666681
ACCGATG19250.041.6103917
ATGAATG20050.041.40897821
Produced by FastQC (version 0.11.5)