##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935848.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 644706 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91211032625724 33.0 31.0 34.0 30.0 34.0 2 32.02931413698647 33.0 31.0 34.0 30.0 34.0 3 31.92893815165362 33.0 31.0 34.0 30.0 34.0 4 35.64496685310824 37.0 35.0 37.0 33.0 37.0 5 32.085525805560984 37.0 35.0 37.0 0.0 37.0 6 33.769508892425385 37.0 35.0 37.0 17.0 37.0 7 35.34762046576269 37.0 35.0 37.0 32.0 37.0 8 35.48533284939182 37.0 35.0 37.0 33.0 37.0 9 37.44387053943968 39.0 37.0 39.0 34.0 39.0 10 37.181239200503796 39.0 37.0 39.0 34.0 39.0 11 37.10733574683654 39.0 37.0 39.0 33.0 39.0 12 37.13701128886655 39.0 37.0 39.0 34.0 39.0 13 37.09018064047798 39.0 37.0 39.0 33.0 39.0 14 38.380039894153306 40.0 38.0 41.0 34.0 41.0 15 38.34971134129355 40.0 38.0 41.0 33.0 41.0 16 38.39809153319498 40.0 38.0 41.0 34.0 41.0 17 38.239448678932725 40.0 37.0 41.0 33.0 41.0 18 38.072121245963274 40.0 37.0 41.0 33.0 41.0 19 37.91714828154228 40.0 37.0 41.0 33.0 41.0 20 37.63343446470174 40.0 36.0 41.0 32.0 41.0 21 37.799736624135654 40.0 36.0 41.0 33.0 41.0 22 37.837589536936214 40.0 36.0 41.0 33.0 41.0 23 37.76087394874563 40.0 36.0 41.0 33.0 41.0 24 37.78666865206777 40.0 36.0 41.0 33.0 41.0 25 37.70688810093283 40.0 35.0 41.0 33.0 41.0 26 37.60097160566212 40.0 35.0 41.0 33.0 41.0 27 37.553200993941424 40.0 35.0 41.0 33.0 41.0 28 37.42288422940069 40.0 35.0 41.0 33.0 41.0 29 37.34911261877507 40.0 35.0 41.0 32.0 41.0 30 37.133315030416966 40.0 35.0 41.0 31.0 41.0 31 36.92144946688878 39.0 35.0 41.0 31.0 41.0 32 36.62935663697871 39.0 35.0 41.0 30.0 41.0 33 36.49227554885483 39.0 35.0 41.0 30.0 41.0 34 36.16006210582808 40.0 35.0 41.0 28.0 41.0 35 36.05531358479679 40.0 35.0 41.0 26.0 41.0 36 35.904381221828245 40.0 35.0 41.0 25.0 41.0 37 35.74779977229931 40.0 35.0 41.0 24.0 41.0 38 35.585960112051076 39.0 35.0 41.0 23.0 41.0 39 35.499875912431406 39.0 35.0 41.0 23.0 41.0 40 35.45162601247701 39.0 35.0 41.0 23.0 41.0 41 35.44347345922017 39.0 35.0 41.0 23.0 41.0 42 35.35926608407553 39.0 35.0 41.0 23.0 41.0 43 35.2861986704017 39.0 35.0 41.0 22.0 41.0 44 35.353337800485804 39.0 35.0 41.0 23.0 41.0 45 35.31771225954156 39.0 35.0 41.0 23.0 41.0 46 35.17807031422075 39.0 35.0 41.0 23.0 41.0 47 34.883844729225416 39.0 34.0 41.0 22.0 41.0 48 34.96049982472631 39.0 35.0 41.0 22.0 41.0 49 34.95843066451995 39.0 35.0 41.0 22.0 41.0 50 34.87435358132234 39.0 35.0 41.0 22.0 41.0 51 33.84410258319296 37.0 33.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 11.0 10 12.0 11 7.0 12 7.0 13 13.0 14 18.0 15 22.0 16 43.0 17 103.0 18 216.0 19 564.0 20 1343.0 21 2651.0 22 4485.0 23 6167.0 24 7464.0 25 8996.0 26 10629.0 27 10759.0 28 10127.0 29 9825.0 30 10938.0 31 13080.0 32 16470.0 33 22075.0 34 33399.0 35 42276.0 36 47125.0 37 69098.0 38 117240.0 39 199407.0 40 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.34173406172736 16.385608323794102 19.942578477631663 16.330079136846873 2 33.10066914221366 32.30821490726005 19.950644169590483 14.640471780935806 3 25.6954332672567 32.335514172351424 28.592567775078876 13.376484785312995 4 22.922231218570946 20.594193322227497 39.8800383430587 16.60353711614286 5 26.593517044978643 31.367941356215077 24.90980384857594 17.12873775023034 6 21.89835366818364 35.89093323158153 28.73170096136844 13.479012138866398 7 69.54906577571792 4.014077734657348 22.489786042009847 3.9470704476148817 8 68.48284334254684 10.807717005891057 15.668692396224015 5.040747255338092 9 63.491420895726115 5.622097514215782 15.690252611267772 15.196228978790332 10 34.436471818162076 29.841664262470026 22.495059763675226 13.226804155692673 11 25.725524502641512 20.74697614106275 33.901964616429815 19.625534739865923 12 20.564877634146416 19.32632859008602 36.54409917078482 23.564694604982737 13 23.249046852363715 21.297924945634133 39.26487422173828 16.188153980263873 14 17.810754049132473 30.06672809001312 33.04700126879539 19.075516592059017 15 15.651320136620415 24.305497389507778 39.71701829981418 20.32616417405763 16 19.088700896222463 27.12724869940717 30.536709756074863 23.247340648295502 17 18.993463687324144 27.912257680244952 32.88785896206953 20.206419670361374 18 19.416136967858215 24.84729473589512 32.62805061531923 23.10851768092743 19 18.08560801357518 25.965323728955525 30.471563782561358 25.477504474907942 20 22.948910045819336 26.643927619721236 34.00418175106172 16.402980583397703 21 19.72790698395858 29.968078472978384 33.716763920298554 16.587250622764486 22 19.343390630768134 20.82902904579762 36.67206447590065 23.155515847533604 23 20.779083799437263 26.130825523571982 34.570641501707755 18.519449175282997 24 21.02741404609233 25.884977028288862 32.132941216616565 20.95466770900224 25 17.98044379918909 33.04467462688419 29.354465446265433 19.62041612766129 26 19.686027429557036 23.63046101633923 33.767329604501896 22.91618194960183 27 25.30455742617565 23.91710329979867 29.56665518856657 21.211684085459108 28 18.250024041966416 26.4688090385385 33.11819651127801 22.162970408217078 29 24.814566639677622 22.227340834426855 32.628671053162215 20.32942147273331 30 24.45161050153093 25.84356280226956 32.07632626344411 17.628500432755395 31 22.47179334456326 22.873681957357306 31.606344597382375 23.048180100697063 32 25.76678361920007 27.681920131036474 29.482120532459753 17.0691757173037 33 26.201400328211623 23.348937345084426 28.083653634369774 22.36600869233418 34 21.987076279730605 23.359174569493693 30.676773599128904 23.9769755516468 35 22.419056127909464 24.840469919622276 32.114172971866246 20.62630098060201 36 21.336392091899253 30.86600714123957 29.76628106454725 18.031319702313922 37 20.424813791092376 26.600341861251486 32.35366197925876 20.621182368397378 38 20.85089947976287 25.694657719952975 29.659410646092947 23.79503215419121 39 22.075023343973843 27.23644575977267 28.476235679519036 22.21229521673445 40 22.547021433025286 23.33078953817709 32.90042282839 21.221766200407625 41 18.841456415792628 27.927458407398102 28.9122483736773 24.318836803131973 42 21.0744122126985 24.295105055637762 33.96959234131527 20.66089039034847 43 23.682112466767798 26.089721516474174 27.74318836803132 22.484977648726705 44 22.340415631311018 23.156446504298085 29.879821189813647 24.62331667457725 45 21.26255998858394 21.82172959457489 29.632111381001575 27.28359903583959 46 25.833325577860293 25.350780045478093 28.002531386399383 20.813362990262227 47 19.14143811287626 24.95912865709331 34.801909707680714 21.09752352234972 48 20.343846652582727 24.922057495974908 30.327622202988646 24.406473648453712 49 20.544868513710124 22.057030646527252 36.22953718439103 21.168563655371596 50 20.575114858555683 23.098590675439656 33.390258505427276 22.93603596057738 51 20.061237215102697 21.61047050903823 30.8734523953554 27.454839880503673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162.0 1 400.5 2 639.0 3 7732.0 4 14825.0 5 9694.0 6 4563.0 7 4337.5 8 4112.0 9 4047.0 10 3982.0 11 3890.5 12 3799.0 13 3640.5 14 3482.0 15 3338.0 16 3194.0 17 3107.5 18 3021.0 19 2935.0 20 2849.0 21 2872.5 22 2896.0 23 2960.0 24 3024.0 25 3632.0 26 5197.5 27 6155.0 28 7282.0 29 8409.0 30 9365.0 31 10321.0 32 11850.0 33 13379.0 34 15226.0 35 17073.0 36 18426.5 37 19780.0 38 22059.5 39 24339.0 40 25820.0 41 27301.0 42 29846.0 43 32391.0 44 37289.0 45 42187.0 46 71503.5 47 100820.0 48 78383.0 49 55946.0 50 56088.5 51 56231.0 52 50085.5 53 43940.0 54 40266.5 55 36593.0 56 32197.0 57 27801.0 58 24277.5 59 20754.0 60 18236.5 61 15719.0 62 13669.0 63 11619.0 64 9820.0 65 8021.0 66 6428.5 67 4836.0 68 3715.0 69 2594.0 70 2044.0 71 1494.0 72 1182.5 73 871.0 74 767.5 75 541.0 76 418.0 77 280.0 78 142.0 79 102.0 80 62.0 81 51.0 82 40.0 83 26.5 84 13.0 85 7.5 86 2.0 87 1.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 644706.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.7775515680645 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26336956924663 27.299331703843176 2 9.455318138439177 7.1439753713468335 3 4.198147650766539 4.757872181015453 4 2.664245847564402 4.025947395854637 5 2.0047223788912696 3.786675152410894 6 1.600613268429527 3.628035017317484 7 1.3566716123883582 3.5876212258551288 8 1.0744533903505413 3.247217468915945 9 0.8962730060018332 3.0473099732968603 >10 4.41682307851574 24.45979427402706 >50 0.036770248391519926 0.9838861004666714 >100 0.02214571774063416 1.590034441914162 >500 0.0041784373095536155 1.1805686082672509 >1k 0.0041784373095536155 3.0203235393215104 >5k 0.0016713749238214464 5.100959296183475 >10k+ 4.178437309553616E-4 3.1404482499634923 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC 19895 3.0859027215505983 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG 9033 1.4011037589226716 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC 8603 1.3344066908016987 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8440 1.3091238487000276 No Hit GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT 6239 0.967727925597094 No Hit GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC 3822 0.5928283589729272 TruSeq Adapter, Index 13 (95% over 21bp) GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT 2859 0.44345794827409707 No Hit GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT 2248 0.3486860677580168 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC 1961 0.3041696525237861 No Hit GAATATGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC 1646 0.25531017238865467 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT 1472 0.22832112621877257 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT 1467 0.22754557891504035 No Hit CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG 1256 0.19481748269753965 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA 1207 0.18721711912096367 No Hit CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC 1196 0.18551091505275272 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT 989 0.15340325667823781 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.1516970526100269 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG 951 0.1475090971698728 No Hit GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC 864 0.13401457408493173 No Hit CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC 727 0.11276457796266824 TruSeq Adapter, Index 13 (95% over 21bp) CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT 678 0.10516421438609228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.653283822393463E-4 0.0 0.0 0.10190691571041684 0.0 2 4.653283822393463E-4 0.0 0.0 0.8991695439471635 0.0 3 4.653283822393463E-4 0.0 0.0 1.2596439307219105 0.0 4 4.653283822393463E-4 0.0 0.0 2.570473983490149 0.0 5 4.653283822393463E-4 0.0 0.0 6.558803547663586 0.0 6 4.653283822393463E-4 0.0 0.0 8.017142697601697 0.0 7 4.653283822393463E-4 0.0 0.0 9.150372417815252 0.0 8 4.653283822393463E-4 0.0 0.0 10.390162337561618 0.0 9 4.653283822393463E-4 0.0 0.0 10.890545457929662 0.0 10 4.653283822393463E-4 0.0 0.0 13.129085195422409 0.0 11 4.653283822393463E-4 0.0 0.0 13.94496095894873 0.0 12 4.653283822393463E-4 0.0 0.0 16.155736102967865 0.0 13 4.653283822393463E-4 0.0 0.0 16.58058091595239 0.0 14 4.653283822393463E-4 0.0 0.0 16.8290662720682 0.0 15 4.653283822393463E-4 0.0 0.0 17.269887359509607 0.0 16 4.653283822393463E-4 0.0 0.0 17.732423771455515 0.0 17 4.653283822393463E-4 0.0 0.0 18.266775863727034 0.0 18 4.653283822393463E-4 0.0 0.0 18.76266080973343 0.0 19 4.653283822393463E-4 0.0 0.0 19.416757405701205 0.0 20 4.653283822393463E-4 0.0 0.0 19.793983614236566 0.0 21 6.20437842985795E-4 0.0 0.0 20.190598505365237 0.0 22 6.20437842985795E-4 0.0 0.0 20.715333811070472 0.0 23 6.20437842985795E-4 0.0 0.0 21.10543410484779 0.0 24 6.20437842985795E-4 0.0 0.0 21.454275282066554 0.0 25 6.20437842985795E-4 0.0 0.0 21.756118292679144 0.0 26 6.20437842985795E-4 0.0 0.0 22.057340865448747 0.0 27 6.20437842985795E-4 0.0 0.0 22.424329849574846 0.0 28 6.20437842985795E-4 0.0 0.0 22.73470388052849 0.0 29 6.20437842985795E-4 0.0 0.0 23.071136300887538 0.0 30 7.755473037322438E-4 0.0 0.0 23.489621625981457 0.0 31 7.755473037322438E-4 0.0 0.0 23.841720101875893 0.0 32 7.755473037322438E-4 0.0 0.0 24.205141568404823 0.0 33 7.755473037322438E-4 0.0 0.0 24.562203547043147 0.0 34 7.755473037322438E-4 0.0 0.0 24.919110416220725 0.0 35 7.755473037322438E-4 0.0 0.0 25.285478962503838 0.0 36 7.755473037322438E-4 0.0 0.0 25.624238024774083 0.0 37 7.755473037322438E-4 0.0 0.0 25.96113577351537 0.0 38 9.306567644786926E-4 0.0 0.0 26.31307913994906 0.0 39 9.306567644786926E-4 0.0 0.0 26.708763374313254 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 25 3.8888928E-5 45.000004 9 GTATACG 25 3.8888928E-5 45.000004 1 GGCGCGA 25 3.8888928E-5 45.000004 8 GATTCGC 25 3.8888928E-5 45.000004 13 CGGGTGA 20 7.030979E-4 45.0 6 CGCGCGA 20 7.030979E-4 45.0 41 CCGACGT 20 7.030979E-4 45.0 44 ATCGACG 20 7.030979E-4 45.0 1 CGGACGG 20 7.030979E-4 45.0 2 CGTTTTT 4235 0.0 43.77804 1 ACGGCTG 1070 0.0 43.317757 8 CCGATGA 1860 0.0 43.185486 18 CGATGAA 1925 0.0 42.66234 19 CATCGAA 185 0.0 42.567566 42 GCGAACC 90 0.0 42.5 33 TAATACG 405 0.0 42.222225 4 GATGAAT 1995 0.0 41.954887 20 CGTTATT 540 0.0 41.666668 1 ACCGATG 1925 0.0 41.61039 17 ATGAATG 2005 0.0 41.408978 21 >>END_MODULE