##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935847.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 711890 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01989633229853 33.0 31.0 34.0 30.0 34.0 2 32.16483164533847 33.0 31.0 34.0 30.0 34.0 3 32.018662995687535 33.0 31.0 34.0 30.0 34.0 4 35.721254688224306 37.0 35.0 37.0 33.0 37.0 5 32.146200958013175 37.0 35.0 37.0 0.0 37.0 6 33.90004776018767 37.0 35.0 37.0 17.0 37.0 7 35.5184298135948 37.0 35.0 37.0 32.0 37.0 8 35.76531065192656 37.0 35.0 37.0 35.0 37.0 9 37.733711668937616 39.0 38.0 39.0 35.0 39.0 10 37.409991712202725 39.0 37.0 39.0 34.0 39.0 11 37.34911854359522 39.0 37.0 39.0 34.0 39.0 12 37.336530924721515 39.0 37.0 39.0 34.0 39.0 13 37.343402772900305 39.0 37.0 39.0 34.0 39.0 14 38.56098273609687 40.0 38.0 41.0 34.0 41.0 15 38.66218516905702 40.0 38.0 41.0 35.0 41.0 16 38.60698001095675 40.0 38.0 41.0 34.0 41.0 17 38.584420345839945 40.0 38.0 41.0 34.0 41.0 18 38.39770891570327 40.0 38.0 41.0 34.0 41.0 19 38.197595134079705 40.0 37.0 41.0 34.0 41.0 20 38.159239489246936 40.0 36.0 41.0 34.0 41.0 21 38.078131452893004 40.0 36.0 41.0 34.0 41.0 22 38.15758895334953 40.0 36.0 41.0 34.0 41.0 23 38.18044641728357 40.0 36.0 41.0 34.0 41.0 24 38.14844147269943 40.0 36.0 41.0 34.0 41.0 25 37.89912065066232 40.0 36.0 41.0 34.0 41.0 26 37.94533144165531 40.0 36.0 41.0 34.0 41.0 27 37.93262020817823 40.0 36.0 41.0 34.0 41.0 28 37.85184368371518 40.0 36.0 41.0 34.0 41.0 29 37.7802595906671 40.0 36.0 41.0 34.0 41.0 30 37.57690513983902 40.0 35.0 41.0 33.0 41.0 31 37.46722948770175 40.0 35.0 41.0 33.0 41.0 32 37.25966933093596 40.0 35.0 41.0 33.0 41.0 33 37.00205649749259 40.0 35.0 41.0 32.0 41.0 34 36.7350770484204 40.0 35.0 41.0 31.0 41.0 35 36.6055682759977 40.0 35.0 41.0 30.0 41.0 36 36.48994648049558 40.0 35.0 41.0 30.0 41.0 37 36.439837615361924 40.0 35.0 41.0 30.0 41.0 38 36.30378007838289 40.0 35.0 41.0 29.0 41.0 39 36.21387012038377 40.0 35.0 41.0 29.0 41.0 40 36.140037084381014 40.0 35.0 41.0 28.0 41.0 41 36.019219261402746 39.0 35.0 41.0 27.0 41.0 42 36.017100956608466 39.0 35.0 41.0 27.0 41.0 43 35.957017235808905 39.0 35.0 41.0 27.0 41.0 44 35.90987090702215 39.0 35.0 41.0 27.0 41.0 45 35.90235710573263 39.0 35.0 41.0 27.0 41.0 46 35.78940285718299 39.0 35.0 41.0 26.0 41.0 47 35.714764921546866 39.0 35.0 41.0 26.0 41.0 48 35.66895306859206 39.0 35.0 41.0 26.0 41.0 49 35.64811557965416 39.0 35.0 41.0 26.0 41.0 50 35.560217168382756 39.0 35.0 41.0 26.0 41.0 51 34.609809099720465 38.0 34.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 12.0 9 18.0 10 30.0 11 26.0 12 20.0 13 16.0 14 19.0 15 37.0 16 53.0 17 97.0 18 202.0 19 385.0 20 715.0 21 1191.0 22 1870.0 23 2870.0 24 4657.0 25 7553.0 26 10833.0 27 12137.0 28 11577.0 29 10794.0 30 11180.0 31 13015.0 32 16528.0 33 22691.0 34 36854.0 35 47141.0 36 50048.0 37 75984.0 38 132722.0 39 240453.0 40 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.21434491283765 19.449212659259153 22.180252567110088 19.1561898607931 2 37.295649608787876 23.41724142774867 22.99175434407001 16.295354619393446 3 29.162511062102293 23.700431246400427 31.900153113542824 15.23690457795446 4 25.648485018752897 23.23238140723988 33.37018359578025 17.748949978226975 5 21.424377361671045 34.32763488741239 27.411538299456378 16.836449451460197 6 23.186447344393095 31.858854598322772 30.320555141946087 14.634142915338044 7 74.0603183076037 4.602817851072497 16.604672070123193 4.732191771200607 8 75.2446304906657 4.886990967705685 15.186475438621136 4.681903103007487 9 69.2486198710475 6.524322577926365 17.035918470550225 7.191139080475917 10 32.44630490665693 32.45501411734959 22.212701400497266 12.885979575496215 11 24.143055809184002 23.15456039556673 34.510809254238715 18.19157454101055 12 22.881344027869474 21.025720265771398 36.17244237171473 19.920493334644398 13 22.57919060529014 21.638595850482517 37.76819452443495 18.014019019792386 14 18.426442287432046 25.819016982960854 36.14743850875838 19.607102220848727 15 17.486690359465648 25.565887988312802 38.49063759850539 18.456784053716163 16 21.49334869151133 26.438354240121363 34.11524252342356 17.953054544943743 17 21.40246386379918 25.326525165404767 33.513745101068984 19.757265869727064 18 21.418056160361854 24.586804141089214 34.52331118571689 19.471828512832037 19 20.289511019960948 26.38947028333029 32.490272373540854 20.830746323167908 20 21.364255713663628 27.066822121395163 32.79130202699855 18.77762013794266 21 21.051707426709182 26.991529590245683 34.41571029232045 17.54105269072469 22 20.509769767801206 23.170855047830425 34.809450898312946 21.50992428605543 23 18.787453117756957 25.656632344884745 35.03659273202321 20.519321805335096 24 20.122209891977693 24.196434842461617 34.85229459607523 20.829060669485454 25 20.653752686510558 27.129753192206664 31.92628074562081 20.29021337566197 26 19.527876497773534 25.908075685850342 32.30021492084451 22.263832895531614 27 20.40006180730169 24.853277894056667 32.79762322830774 21.949037070333898 28 18.087204483838796 25.917487252244026 33.34082512747756 22.65448313643962 29 19.499220385171867 26.02059306915394 32.4287460141314 22.051440531542795 30 20.053940917838432 25.097135793451237 32.678784643694954 22.17013864501538 31 20.301029653457697 24.878984112714043 31.84803832052705 22.971947913301214 32 20.28164463610951 25.868603295452946 31.142030369860514 22.70772169857703 33 19.63730351599264 26.23326637542317 29.995785865793874 24.133644242790318 34 18.20056469398362 26.37134950624394 31.642809984688647 23.785275815083793 35 17.825225807357878 26.821419039458345 29.68295663655902 25.670398516624758 36 18.375451263537908 29.992555029569175 28.85459832277459 22.777395384118332 37 18.694461222941747 27.451151160993977 29.652474399134697 24.201913216929583 38 18.519293711107053 28.257876919186952 29.49261824158227 23.730211128123727 39 19.714562643104973 26.570818525333973 29.06193372571605 24.652685105845006 40 20.703619941283062 25.36262624843726 31.108457767351698 22.82529604292798 41 18.596131424798777 26.92986275969602 29.565944176768884 24.908061638736324 42 19.715405469946198 26.92031072216213 30.24624590877804 23.118037899113627 43 20.43644383261459 25.982806332439 30.085125510963774 23.49562432398264 44 20.28318981865176 25.39619885094607 30.115607748388097 24.205003582014076 45 20.321257497647107 24.28127941114498 29.673966483585946 25.723496607621964 46 20.971779347932966 25.528944078439086 29.217154335641744 24.282122237986208 47 19.00672856761578 25.261627498630407 31.805756507325572 23.92588742642824 48 18.655972130525782 25.22398123305567 31.541108879180772 24.578937757237775 49 20.27560437708073 23.858461279130204 32.18179774965233 23.684136594136735 50 18.69094944443664 24.248971048898007 33.05960190478866 24.000477601876693 51 18.617904451530435 23.398277823821097 31.626234390144546 26.357583334503925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 199.0 1 438.0 2 677.0 3 9925.5 4 19174.0 5 12346.5 6 5519.0 7 5311.5 8 5104.0 9 5201.5 10 5299.0 11 5138.5 12 4978.0 13 4767.5 14 4557.0 15 4321.5 16 4086.0 17 3877.0 18 3668.0 19 3572.0 20 3476.0 21 3436.5 22 3397.0 23 3594.5 24 3792.0 25 4389.0 26 5690.5 27 6395.0 28 7456.0 29 8517.0 30 10197.0 31 11877.0 32 13498.0 33 15119.0 34 17138.0 35 19157.0 36 20929.0 37 22701.0 38 24951.0 39 27201.0 40 29649.5 41 32098.0 42 34695.5 43 37293.0 44 41984.5 45 46676.0 46 60443.0 47 74210.0 48 72867.0 49 71524.0 50 69809.0 51 68094.0 52 59841.5 53 51589.0 54 45707.0 55 39825.0 56 35207.5 57 30590.0 58 27596.0 59 24602.0 60 21554.0 61 18506.0 62 16169.0 63 13832.0 64 11513.0 65 9194.0 66 7504.0 67 5814.0 68 4693.0 69 3572.0 70 2720.0 71 1868.0 72 1512.0 73 1156.0 74 969.0 75 585.5 76 389.0 77 305.5 78 222.0 79 160.5 80 99.0 81 75.0 82 51.0 83 32.0 84 13.0 85 7.5 86 2.0 87 4.0 88 6.0 89 4.0 90 2.0 91 1.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 711890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.56367428519917 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43422131394132 29.38198160480747 2 9.754671928143363 7.913706697043662 3 4.177318338044854 5.083421413501229 4 2.6011149806285734 4.220431234102784 5 1.8701546342838937 3.793017172402381 6 1.4814998527802412 3.6057046489048936 7 1.2163428390367605 3.4537534319275114 8 1.012137910729118 3.2844826034014347 9 0.8383459588805082 3.0605753172907724 >10 4.549812732948287 28.202462587251 >50 0.038137864652284685 1.055232410501208 >100 0.0199436509074471 1.4831442256901821 >500 0.0017494430620566155 0.5340738489255392 >1k 0.004198663348935878 3.3571572741123314 >5k 0.0 0.0 >10k+ 3.498886124113231E-4 1.5708555301376208 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11068 1.554734579780584 No Hit GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC 4864 0.6832516259534478 No Hit CGTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT 2831 0.3976737979182177 No Hit CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC 2142 0.3008891823174929 No Hit CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG 1895 0.26619281068704437 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG 1725 0.24231271685232267 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC 1703 0.23922235176782933 No Hit GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC 1695 0.23809858264619535 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT 1602 0.22503476660720054 No Hit GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT 1502 0.21098765258677604 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1334 0.18738850103246288 No Hit CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC 1187 0.16673924342243887 No Hit CGTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT 1174 0.1649131185997837 No Hit CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT 947 0.13302616977342005 No Hit GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT 846 0.11883858461279131 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC 730 0.10254393234909887 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.023557010212252E-4 0.0 0.0 0.13836407310118137 0.0 2 7.023557010212252E-4 0.0 0.0 0.6388627456489064 0.0 3 7.023557010212252E-4 0.0 0.0 1.1042436331455703 0.0 4 7.023557010212252E-4 0.0 0.0 1.7515346472067315 0.0 5 7.023557010212252E-4 0.0 0.0 3.532287291575946 0.0 6 7.023557010212252E-4 0.0 0.0 5.18366601581705 0.0 7 7.023557010212252E-4 0.0 0.0 6.3594094593265815 0.0 8 7.023557010212252E-4 0.0 0.0 7.827192403320738 0.0 9 7.023557010212252E-4 0.0 0.0 8.645717737290873 0.0 10 7.023557010212252E-4 0.0 0.0 9.930466785598899 0.0 11 7.023557010212252E-4 0.0 0.0 11.03541277444549 0.0 12 8.428268412254702E-4 0.0 0.0 12.203992189804605 0.0 13 8.428268412254702E-4 0.0 0.0 12.74691314669401 0.0 14 8.428268412254702E-4 0.0 0.0 13.067608759780303 0.0 15 8.428268412254702E-4 0.0 0.0 13.408110803635394 0.0 16 8.428268412254702E-4 0.0 0.0 13.886555507171051 0.0 17 8.428268412254702E-4 0.0 0.0 14.433690598266587 0.0 18 8.428268412254702E-4 0.0 0.0 15.025355040806867 0.0 19 8.428268412254702E-4 0.0 0.0 15.489471688041691 0.0 20 8.428268412254702E-4 0.0 0.0 15.92254421329138 0.0 21 9.832979814297152E-4 0.0 0.0 16.39270111955499 0.0 22 0.0011237691216339602 0.0 0.0 16.92747475031255 0.0 23 0.0011237691216339602 0.0 0.0 17.442161008020904 0.0 24 0.0011237691216339602 0.0 0.0 17.87256458160671 0.0 25 0.0011237691216339602 0.0 0.0 18.256753150065318 0.0 26 0.0011237691216339602 0.0 0.0 18.627877902484933 0.0 27 0.0012642402618382054 0.0 0.0 19.02779923864642 0.0 28 0.0012642402618382054 0.0 0.0 19.42926575735015 0.0 29 0.0014047114020424504 0.0 0.0 19.84716739945778 0.0 30 0.0014047114020424504 0.0 0.0 20.354127744454903 0.0 31 0.0015451825422466954 0.0 0.0 20.76346064701007 0.0 32 0.0015451825422466954 0.0 0.0 21.218868083552234 0.0 33 0.0015451825422466954 0.0 0.0 21.646883647754567 0.0 34 0.0015451825422466954 0.0 0.0 22.07419685625588 0.0 35 0.0015451825422466954 0.0 0.0 22.529323350517636 0.0 36 0.0015451825422466954 0.0 0.0 22.942870387278933 0.0 37 0.0015451825422466954 0.0 0.0 23.367374172976163 0.0 38 0.0015451825422466954 0.0 0.0 23.86239447105592 0.0 39 0.0015451825422466954 0.0 0.0 24.437904732472713 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACAC 40 6.8084773E-9 45.000004 34 TTTCGCG 20 7.031474E-4 45.000004 1 CTAGTCG 25 3.889303E-5 45.000004 1 CGTTTTT 5405 0.0 43.959297 1 CGTTATT 675 0.0 43.333332 1 CGTAAGG 100 0.0 42.750004 2 GCGCGAC 95 0.0 42.63158 9 TTAACGG 70 0.0 41.785713 2 CGGCGAA 55 6.002665E-11 40.909092 31 TTGTGCG 55 6.002665E-11 40.909092 1 GCGTTAG 55 6.002665E-11 40.909092 1 TTGCACG 50 1.0804797E-9 40.500004 1 AAGGGCG 260 0.0 38.07692 5 ATTTACG 30 1.1394853E-4 37.500004 1 CGACGGT 30 1.1394853E-4 37.500004 28 TTCGGCG 30 1.1394853E-4 37.500004 1 AGGGCGC 120 0.0 37.500004 6 GCCGTTA 30 1.1394853E-4 37.500004 29 GCGTAAG 60 1.546141E-10 37.500004 1 TAGTACG 60 1.546141E-10 37.500004 1 >>END_MODULE