##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787186
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01548935067443	33.0	31.0	34.0	30.0	34.0
2	32.165471692840065	33.0	31.0	34.0	30.0	34.0
3	31.99593615740117	33.0	31.0	34.0	30.0	34.0
4	35.70636164769191	37.0	35.0	37.0	33.0	37.0
5	32.01668855899368	37.0	35.0	37.0	0.0	37.0
6	33.843941584326956	37.0	35.0	37.0	17.0	37.0
7	35.53320180999154	37.0	35.0	37.0	32.0	37.0
8	35.804624320046344	37.0	35.0	37.0	35.0	37.0
9	37.78632496004756	39.0	38.0	39.0	35.0	39.0
10	37.387090217559766	39.0	37.0	39.0	34.0	39.0
11	37.35212135378424	39.0	37.0	39.0	34.0	39.0
12	37.44082465897513	39.0	37.0	39.0	35.0	39.0
13	37.48286427858219	39.0	37.0	39.0	35.0	39.0
14	38.75901502313303	40.0	38.0	41.0	35.0	41.0
15	38.86145586938792	40.0	38.0	41.0	35.0	41.0
16	38.777804737381	40.0	38.0	41.0	35.0	41.0
17	38.773366142182404	40.0	38.0	41.0	35.0	41.0
18	38.55284773865389	40.0	38.0	41.0	35.0	41.0
19	38.30328791416514	40.0	37.0	41.0	34.0	41.0
20	38.25567019738664	40.0	37.0	41.0	34.0	41.0
21	38.162123056050284	40.0	36.0	41.0	34.0	41.0
22	38.23176479256491	40.0	36.0	41.0	34.0	41.0
23	38.23338448600458	40.0	36.0	41.0	34.0	41.0
24	38.18641083555856	40.0	36.0	41.0	34.0	41.0
25	37.92443844275686	40.0	36.0	41.0	34.0	41.0
26	37.986447929714195	40.0	36.0	41.0	34.0	41.0
27	37.967604860858806	40.0	36.0	41.0	34.0	41.0
28	37.89015810748667	40.0	36.0	41.0	34.0	41.0
29	37.8287647392103	40.0	36.0	41.0	34.0	41.0
30	37.58055148338512	40.0	36.0	41.0	33.0	41.0
31	37.42843241622691	40.0	35.0	41.0	33.0	41.0
32	37.167228583841684	40.0	35.0	41.0	32.0	41.0
33	36.83117585932677	40.0	35.0	41.0	31.0	41.0
34	36.497552039797455	40.0	35.0	41.0	30.0	41.0
35	36.31906436344142	40.0	35.0	41.0	29.0	41.0
36	36.168695835545854	40.0	35.0	41.0	27.0	41.0
37	36.08369305348418	40.0	35.0	41.0	27.0	41.0
38	35.967970721024	40.0	35.0	41.0	26.0	41.0
39	35.881038280660476	40.0	35.0	41.0	25.0	41.0
40	35.81802902998783	39.0	35.0	41.0	25.0	41.0
41	35.687484279446025	39.0	35.0	41.0	24.0	41.0
42	35.67687052361195	39.0	35.0	41.0	24.0	41.0
43	35.62003516322699	39.0	35.0	41.0	23.0	41.0
44	35.57972575731784	39.0	35.0	41.0	23.0	41.0
45	35.56893796383574	39.0	35.0	41.0	23.0	41.0
46	35.45433607813147	39.0	35.0	41.0	23.0	41.0
47	35.3799673774686	39.0	35.0	41.0	23.0	41.0
48	35.33170051296644	39.0	35.0	41.0	23.0	41.0
49	35.28975489909627	39.0	35.0	41.0	23.0	41.0
50	35.19353748669311	39.0	35.0	41.0	23.0	41.0
51	34.238150830934494	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	13.0
9	24.0
10	22.0
11	17.0
12	20.0
13	17.0
14	14.0
15	31.0
16	58.0
17	104.0
18	207.0
19	458.0
20	814.0
21	1339.0
22	2222.0
23	3460.0
24	6018.0
25	10285.0
26	14252.0
27	15516.0
28	13868.0
29	12483.0
30	12730.0
31	14284.0
32	17675.0
33	23090.0
34	35916.0
35	47610.0
36	55293.0
37	86058.0
38	150403.0
39	262696.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.635849722937145	19.196479612188224	22.33309535484625	19.83457531002838
2	39.83315252049706	21.902574486843008	22.749388327536312	15.514884665123619
3	29.14470023603062	22.190308262596133	34.11925516968036	14.54573633169289
4	24.809511348016862	23.65425706249857	34.42553602325245	17.110695566232124
5	20.98919442164876	34.96200389742704	28.30284075174101	15.745960929183193
6	22.97373174827804	31.163155848808287	31.33325541866852	14.529856984245146
7	74.26631063052443	4.502366657943612	17.041842715698703	4.189479995833259
8	75.85691310567006	4.156705022701115	15.86550065676981	4.120881214859004
9	69.72989865165285	6.416907821023239	17.8739205219605	5.9792730053634084
10	30.902353446326536	31.527872700988073	24.612734474444416	12.957039378240975
11	21.80005741972037	23.469294423427243	36.724865533685815	18.00578262316657
12	20.209963083693054	21.47154547972144	38.6615107484127	19.65698068817281
13	20.52196050234633	22.07699323920903	40.17525718191126	17.225789076533374
14	18.361225936436878	25.539199121935603	37.672036850248865	18.427538091378658
15	17.45432464500131	25.621263589545546	39.115660085418185	17.80875168003496
16	20.448026260629636	26.093197795692504	36.28087897904688	17.177896964630975
17	20.7017147154548	25.387265525555584	35.06032881682347	18.850690942166146
18	20.828495425477588	24.616291448272708	35.944871987052615	18.61034113919709
19	19.51406656114311	26.51596954214125	34.37154624193012	19.59841765478553
20	20.20132471868148	26.840035264854812	34.43353921436611	18.5251008020976
21	20.17667997144258	26.673111564484124	35.935979552481875	17.214228911591416
22	19.829493918845102	23.389135477511033	36.0583140452193	20.723056558424567
23	17.97072102400195	25.91420579126153	36.349096655682395	19.765976529054125
24	18.56473565332717	24.995109160986097	36.27655979654109	20.16359538914564
25	19.473288396897303	27.005815652209264	34.05929475371767	19.461601197175764
26	19.29823447063337	26.13893031634201	33.74666216116648	20.81617305185814
27	19.457790153788306	25.416356490079856	34.31311024332241	20.812743112809425
28	18.45624795156418	25.792252402862854	34.89137255997947	20.860127085593493
29	19.30598359218787	24.143722068227838	34.22825101056167	22.32204332902262
30	20.294441212115053	24.98050016133417	34.114554882835826	20.610503743714954
31	19.98549262817174	25.686051327132343	33.93708729575983	20.391368748936085
32	20.993513604154547	25.137515148897467	33.86937775824265	19.99959348870534
33	22.094269969232176	25.197094460521402	32.43845800103152	20.2701775692149
34	21.02133422088299	24.73329048026769	34.32187564311357	19.923499655735746
35	20.82036519958434	25.65784960606515	33.73967524828947	19.782109946061034
36	22.02910112730663	26.101200986806166	32.63841074409351	19.23128714179368
37	21.650029345034085	26.027012675530308	33.10462838515929	19.21832959427632
38	21.568981155660797	25.820962263048376	33.08785979425447	19.522196787036357
39	22.82967430823211	24.649701595302762	31.855622432309517	20.665001664155614
40	22.884807402570676	24.209780153610456	33.57351375659628	19.331898687222587
41	20.581920918309017	25.64501909332737	32.69951447307244	21.073545515291176
42	21.343113317564082	25.92233601715478	32.665342117364894	20.069208547916247
43	21.646853475544532	25.59306186847835	32.1091838523551	20.650900803622015
44	21.767028377029064	25.324891448780846	31.219686325722257	21.688393848467832
45	21.529092234872063	24.40058639254255	31.20507732607033	22.865244046515055
46	22.123233898976864	24.988757422006998	30.782432614401174	22.105576064614972
47	20.5496540842952	25.280429275927162	32.613003788177124	21.55691285160051
48	20.32276996796183	24.979610917877096	32.82502483529941	21.87259427886167
49	21.468242575452308	23.87466240507326	32.858180912770294	21.798914106704135
50	20.194337805804473	23.990772193611168	33.8634071236023	21.95148287698206
51	20.15508405891365	22.95543874001824	32.81994344411613	24.069533756951976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	421.0
2	601.0
3	11191.5
4	21782.0
5	14472.0
6	7162.0
7	6904.0
8	6646.0
9	6725.5
10	6805.0
11	6753.5
12	6702.0
13	6523.5
14	6345.0
15	6117.5
16	5890.0
17	5614.0
18	5338.0
19	5064.5
20	4791.0
21	4641.5
22	4492.0
23	4815.0
24	5138.0
25	5739.5
26	7170.5
27	8000.0
28	9691.0
29	11382.0
30	12660.0
31	13938.0
32	16487.5
33	19037.0
34	21581.5
35	24126.0
36	26342.0
37	28558.0
38	31095.5
39	33633.0
40	36055.5
41	38478.0
42	42012.0
43	45546.0
44	49387.5
45	53229.0
46	65792.5
47	78356.0
48	78189.0
49	78022.0
50	75406.0
51	72790.0
52	63408.0
53	54026.0
54	47136.5
55	40247.0
56	34832.0
57	29417.0
58	26028.5
59	22640.0
60	19796.0
61	16952.0
62	14379.5
63	11807.0
64	9713.5
65	7620.0
66	6116.0
67	4612.0
68	3593.5
69	2575.0
70	2094.5
71	1614.0
72	1283.0
73	952.0
74	775.0
75	503.5
76	409.0
77	320.5
78	232.0
79	138.0
80	44.0
81	40.0
82	36.0
83	27.0
84	18.0
85	14.5
86	11.0
87	7.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	787186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.79780372939031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42044836457343	31.422339388308494
2	9.893967904920276	8.4688019299933
3	4.184536540069252	5.372669206210375
4	2.596375943503634	4.444767521511165
5	1.7578519180546204	3.7616100687116973
6	1.3613989028512237	3.495892982498038
7	1.0923758870016955	3.272590216743205
8	0.9079579683553193	3.1086885539365547
9	0.7299632854414462	2.811674287798554
>10	3.9933457719684617	26.720850503767977
>50	0.038200618713119854	1.112627405187364
>100	0.01790650218803749	1.1976913375484826
>500	0.0017906502187662325	0.5614856691680733
>1k	0.003581300437532465	2.6811068429269853
>5k	0.0	0.0
>10k+	2.984417031277054E-4	1.5672040856897769
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12270	1.558716745470575	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	3233	0.41070344238845713	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	3010	0.3823746865416814	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	2414	0.30666195791083684	No Hit
CGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG	1994	0.25330735048641617	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	1986	0.25229107224976055	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1378	0.17505392626393254	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	1350	0.1714969524356378	TruSeq Adapter, Index 22 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1328	0.16870218728483485	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	1173	0.14901179644963197	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	1076	0.13668942283018246	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1029	0.13071878818983063	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	1020	0.12957547517359302	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	984	0.12500222310864267	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	889	0.11293391904835706	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	875	0.11115543213420971	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.081391183278158E-4	0.0	0.0	0.13249727510397796	0.0
2	5.081391183278158E-4	0.0	0.0	0.5388815349866486	0.0
3	5.081391183278158E-4	0.0	0.0	0.9593666554029162	0.0
4	5.081391183278158E-4	0.0	0.0	1.5185737551226774	0.0
5	5.081391183278158E-4	0.0	0.0	2.9610536772757645	0.0
6	5.081391183278158E-4	0.0	0.0	4.646932237107875	0.0
7	5.081391183278158E-4	0.0	0.0	5.853762643136438	0.0
8	5.081391183278158E-4	0.0	0.0	7.29979953911782	0.0
9	6.351738979097697E-4	0.0	0.0	8.124001189045536	0.0
10	6.351738979097697E-4	0.0	0.0	9.301486560990668	0.0
11	6.351738979097697E-4	0.0	0.0	10.365529874769114	0.0
12	6.351738979097697E-4	0.0	0.0	11.383332528779729	0.0
13	7.622086774917236E-4	0.0	0.0	11.946350671886949	0.0
14	8.892434570736776E-4	0.0	0.0	12.26431872518058	0.0
15	8.892434570736776E-4	0.0	0.0	12.576189109054276	0.0
16	8.892434570736776E-4	0.0	0.0	13.031989898194327	0.0
17	8.892434570736776E-4	0.0	0.0	13.580526076429205	0.0
18	8.892434570736776E-4	0.0	0.0	14.19130929665924	0.0
19	0.0010162782366556315	0.0	0.0	14.626657486286595	0.0
20	0.0012703477958195394	0.0	0.0	15.049429232735339	0.0
21	0.0012703477958195394	0.0	0.0	15.51564687380111	0.0
22	0.0012703477958195394	0.0	0.0	16.0260726181614	0.0
23	0.0012703477958195394	0.0	0.0	16.548693701361557	0.0
24	0.0013973825754014933	0.0	0.0	16.96816254354117	0.0
25	0.0013973825754014933	0.0	0.0	17.367559890546833	0.0
26	0.0013973825754014933	0.0	0.0	17.746631672819383	0.0
27	0.0013973825754014933	0.0	0.0	18.159494706460734	0.0
28	0.0015244173549834473	0.0	0.0	18.57324698355916	0.0
29	0.0015244173549834473	0.0	0.0	18.994494312652918	0.0
30	0.0015244173549834473	0.0	0.0	19.524102308730082	0.0
31	0.0015244173549834473	0.0	0.0	19.95995863747577	0.0
32	0.0015244173549834473	0.0	0.0	20.415759426615818	0.0
33	0.0015244173549834473	0.0	0.0	20.88134189378368	0.0
34	0.0015244173549834473	0.0	0.0	21.319738918121004	0.0
35	0.0015244173549834473	0.0	0.0	21.79230829816587	0.0
36	0.0016514521345654012	0.0	0.0	22.21812887932458	0.0
37	0.0017784869141473552	0.0	0.0	22.663004677420584	0.0
38	0.0017784869141473552	0.0	0.0	23.11588366663025	0.0
39	0.0017784869141473552	0.0	0.0	23.661243975375577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTGC	25	3.8896807E-5	45.000004	26
CGTTAGG	65	0.0	45.000004	2
GACGTAG	20	7.0319313E-4	45.0	1
CGTTATG	20	7.0319313E-4	45.0	1
GTATGCG	35	1.2112832E-7	45.0	1
GTAAGCG	20	7.0319313E-4	45.0	1
TTCGACG	20	7.0319313E-4	45.0	1
GCGTTAG	30	2.1646265E-6	44.999996	1
CGTTTTT	7145	0.0	43.80336	1
CGTTATT	1005	0.0	43.432835	1
TCGACGG	50	1.0804797E-9	40.500004	2
TAATACG	45	1.9270374E-8	40.0	4
GAGTACG	45	1.9270374E-8	40.0	1
ACGTAAG	40	3.456371E-7	39.375	1
TAGTGCG	40	3.456371E-7	39.375	1
CGTAAGG	120	0.0	39.374996	2
TACGGGA	475	0.0	39.31579	4
TGTACGG	115	0.0	39.130436	2
TTGTGCG	70	0.0	38.571426	1
TACAACG	35	6.245782E-6	38.571426	1
>>END_MODULE