##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935845.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516491
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04097457651731	33.0	31.0	34.0	30.0	34.0
2	32.15924575646042	33.0	31.0	34.0	30.0	34.0
3	32.052728895566425	33.0	31.0	34.0	30.0	34.0
4	35.73769920482641	37.0	35.0	37.0	33.0	37.0
5	32.115306946297224	37.0	35.0	37.0	0.0	37.0
6	33.89012586860178	37.0	35.0	37.0	17.0	37.0
7	35.52553481086795	37.0	35.0	37.0	32.0	37.0
8	35.78950068829854	37.0	35.0	37.0	35.0	37.0
9	37.730136633552185	39.0	38.0	39.0	35.0	39.0
10	37.37472095351129	39.0	37.0	39.0	34.0	39.0
11	37.35815532119631	39.0	37.0	39.0	34.0	39.0
12	37.325783024292775	39.0	37.0	39.0	34.0	39.0
13	37.31058430834226	39.0	37.0	39.0	34.0	39.0
14	38.49033961869616	40.0	38.0	41.0	34.0	41.0
15	38.61813661806305	40.0	38.0	41.0	34.0	41.0
16	38.558836456007946	40.0	38.0	41.0	34.0	41.0
17	38.570375863277384	40.0	38.0	41.0	34.0	41.0
18	38.41104104427764	40.0	38.0	41.0	34.0	41.0
19	38.23871664753113	40.0	37.0	41.0	34.0	41.0
20	38.27019057447274	40.0	37.0	41.0	34.0	41.0
21	38.152145923162266	40.0	37.0	41.0	34.0	41.0
22	38.236066068915044	40.0	37.0	41.0	34.0	41.0
23	38.24222687326594	40.0	37.0	41.0	34.0	41.0
24	38.20431914592897	40.0	37.0	41.0	34.0	41.0
25	37.92994069596566	40.0	36.0	41.0	34.0	41.0
26	38.00287129882224	40.0	36.0	41.0	34.0	41.0
27	37.98729116286634	40.0	36.0	41.0	34.0	41.0
28	37.90089469129181	40.0	36.0	41.0	34.0	41.0
29	37.844599421867954	40.0	36.0	41.0	34.0	41.0
30	37.656205045199236	40.0	36.0	41.0	33.0	41.0
31	37.563547089881524	40.0	36.0	41.0	33.0	41.0
32	37.41651451816198	40.0	36.0	41.0	33.0	41.0
33	37.21157580674203	40.0	35.0	41.0	32.0	41.0
34	36.9918507776515	40.0	35.0	41.0	31.0	41.0
35	36.903663374579615	40.0	35.0	41.0	31.0	41.0
36	36.77609483998753	40.0	35.0	41.0	31.0	41.0
37	36.70190187244308	40.0	35.0	41.0	31.0	41.0
38	36.55193023692571	39.0	35.0	41.0	30.0	41.0
39	36.45550067668169	39.0	35.0	41.0	30.0	41.0
40	36.367049958276134	39.0	35.0	41.0	30.0	41.0
41	36.22179863734315	39.0	35.0	41.0	30.0	41.0
42	36.204812862179594	39.0	35.0	41.0	30.0	41.0
43	36.13652706436317	39.0	35.0	41.0	29.0	41.0
44	36.07634595762559	39.0	35.0	41.0	29.0	41.0
45	36.03767732642002	39.0	35.0	41.0	29.0	41.0
46	35.93001620550987	39.0	35.0	41.0	29.0	41.0
47	35.84205339492847	39.0	35.0	41.0	28.0	41.0
48	35.78521987798432	39.0	35.0	41.0	28.0	41.0
49	35.75584860142771	39.0	35.0	41.0	28.0	41.0
50	35.64713809146723	38.0	35.0	41.0	28.0	41.0
51	34.61012679795001	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	22.0
10	25.0
11	25.0
12	10.0
13	10.0
14	12.0
15	10.0
16	38.0
17	65.0
18	121.0
19	260.0
20	461.0
21	725.0
22	1186.0
23	1811.0
24	2868.0
25	4497.0
26	6261.0
27	7084.0
28	7035.0
29	7138.0
30	7859.0
31	9326.0
32	12196.0
33	17666.0
34	28970.0
35	35915.0
36	39593.0
37	61603.0
38	101811.0
39	161764.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42937631052622	19.431509939185776	23.325672664189696	15.813441086098306
2	38.22660995060902	22.283834568269338	22.664480116788095	16.82507536433355
3	30.625122218973804	23.704188456333217	29.227808422605623	16.442880902087357
4	26.933673578048793	25.451943983535045	29.04116431844892	18.57321811996724
5	21.80676914021735	35.31987972684906	25.804515470743922	17.06883566218966
6	24.79501094888391	32.168614748369286	27.878317337572195	15.15805696517461
7	76.81818269824645	5.506001072622756	12.856952008844297	4.81886422028651
8	77.92856022660608	4.440348428142988	13.046693940455883	4.584397404795049
9	71.71005883936023	7.147849623710771	14.593090683090315	6.549000853838692
10	34.87340534491404	28.97998222621498	20.759316232035022	15.387296196835957
11	27.39060312764404	24.38532326797563	29.110865436183786	19.11320816819654
12	24.512915036273622	22.200967683851218	33.04801051712421	20.23810676275095
13	23.61280254641417	22.339208234025378	34.88715195424509	19.16083726531537
14	20.396870419813702	25.499766694869802	33.846281929404384	20.25708095591211
15	19.11979105153817	25.12028283164663	35.46141171869404	20.298514398121167
16	22.812014149326902	25.40295958690471	32.231926596978454	19.553099666789933
17	22.85170506359259	24.47845170583805	30.727156910769015	21.942686319800345
18	23.661012486180784	23.93226600269898	31.999589537862228	20.407131973258004
19	22.44840665181	25.869569847296468	30.218725979736337	21.463297521157195
20	23.505733885004773	26.436278657324134	29.760053902197715	20.297933555473378
21	23.31715363868877	26.099002693173745	31.772673676792046	18.811169991345444
22	22.8902342925627	23.031959898623597	31.613910019729293	22.463895789084418
23	21.255743081680027	25.5863122493906	31.571895734872435	21.586048934056933
24	21.065807535852514	25.072266506095943	31.81914108861529	22.042784869436254
25	22.09002673812322	26.02465483425655	29.92114092985163	21.964177497768596
26	21.191656776207136	25.7607586579437	30.091908668302057	22.955675897547103
27	21.80889889659258	24.37835315620214	31.07759864160266	22.735149305602615
28	19.872175894642886	25.551461690523166	31.053784093043248	23.5225783217907
29	20.755637561932346	25.314477890224612	31.144395546098576	22.785489001744462
30	21.89776782170454	23.752398396099835	30.848746638373175	23.501087143822446
31	21.740746692585155	26.026203747983995	28.86187755449756	23.371172004933292
32	21.658847879246686	27.093792534623063	28.60785570319715	22.639503882933102
33	21.62264202086774	25.79948150112974	27.564468693549355	25.013407784453168
34	20.184282010722356	25.99503185921923	29.4367181615943	24.383967968464116
35	21.031731433848798	25.977025737137723	28.457611071635323	24.533631757378156
36	22.1097753881481	27.4589489458674	27.16484895186944	23.266426714115056
37	20.904914122414525	28.3215002778364	27.289149278496623	23.484436321252453
38	21.391660261263024	28.40862667500498	27.024865873751914	23.174847189980078
39	21.640260914517388	26.241696370314294	26.874621242190088	25.243421472978234
40	23.646878648417882	24.221525641298687	28.95713574873521	23.174459961548216
41	21.4729782319537	25.55959348759223	28.116850051598192	24.850578228855877
42	21.805026612273977	26.5905891874205	28.752098294065142	22.85228590624038
43	21.465427277532427	25.620001122962456	29.34688116540269	23.567690434102435
44	21.920033456536515	25.021926809954092	28.421792441688236	24.636247291821153
45	21.974051822781036	23.67785692296671	28.275613708660945	26.072477545591305
46	22.276864456495854	24.595007463828026	27.96931601905938	25.158812060616736
47	21.028439992177987	25.608190655790715	29.9087496200321	23.454619731999202
48	20.845087329692095	25.172365055731856	29.77321966888097	24.209327945695087
49	22.084411925861243	24.367704374325978	29.718620459988653	23.829263239824122
50	20.338786155034647	24.107099639683945	30.9232881115063	24.63082609377511
51	20.812947369847684	23.087720792811492	29.21580434121795	26.883527496122877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	357.0
2	566.0
3	4628.5
4	8691.0
5	5669.5
6	2648.0
7	2579.5
8	2511.0
9	2547.5
10	2584.0
11	2546.0
12	2508.0
13	2444.5
14	2381.0
15	2348.5
16	2316.0
17	2177.5
18	2039.0
19	2006.5
20	1974.0
21	1920.0
22	1866.0
23	1923.0
24	1980.0
25	2426.5
26	3436.5
27	4000.0
28	4600.0
29	5200.0
30	6029.0
31	6858.0
32	7780.0
33	8702.0
34	10052.0
35	11402.0
36	12710.5
37	14019.0
38	15414.5
39	16810.0
40	19116.0
41	21422.0
42	24017.5
43	26613.0
44	30672.0
45	34731.0
46	40385.0
47	46039.0
48	48966.5
49	51894.0
50	51521.0
51	51148.0
52	45946.0
53	40744.0
54	36365.0
55	31986.0
56	28756.0
57	25526.0
58	23868.0
59	22210.0
60	20022.0
61	17834.0
62	15788.0
63	13742.0
64	12049.0
65	10356.0
66	8766.0
67	7176.0
68	5987.0
69	4798.0
70	3936.0
71	3074.0
72	2678.0
73	2282.0
74	1860.5
75	1074.5
76	710.0
77	563.0
78	416.0
79	288.5
80	161.0
81	106.0
82	51.0
83	41.5
84	32.0
85	21.0
86	10.0
87	12.0
88	14.0
89	10.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	516491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.92369366606274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25447199675754	31.014288219919294
2	9.794431953510067	8.408263936111252
3	4.172977056898479	5.3735876659745525
4	2.6535186352999647	4.555952841552181
5	1.955299208154505	4.196433211815951
6	1.5979681460424695	4.11544171133119
7	1.3223822842847732	3.9733072456047527
8	1.1606508287113564	3.9855536499894493
9	0.9306111290928435	3.595074032466898
>10	4.088089002182304	24.69213184242376
>50	0.043215536676593426	1.2670424527511852
>100	0.02047051737085648	1.8012739444655423
>500	0.004094103474171297	1.3355787295549388
>1k	0.0018196015440761317	1.6860705160390197
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4967	0.9616818105252559	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT	1332	0.2578941356190137	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	1170	0.22652863263832282	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1166	0.2257541757746021	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	852	0.16495931197252228	No Hit
TGAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT	836	0.1618614845176392	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	835	0.16166787030170904	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	834	0.16147425608577884	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	780	0.15101908842554854	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	763	0.14772764675473532	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT	747	0.14462981929985227	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	633	0.12255779868381056	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.10842396092090666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8722843186038094E-4	0.0	0.0	0.15915088549461656	0.0
2	3.8722843186038094E-4	0.0	0.0	0.5891680590755696	0.0
3	3.8722843186038094E-4	0.0	0.0	1.0350615983627982	0.0
4	3.8722843186038094E-4	0.0	0.0	1.4925719906058383	0.0
5	3.8722843186038094E-4	0.0	0.0	2.85658414183403	0.0
6	3.8722843186038094E-4	0.0	0.0	4.344122162825683	0.0
7	3.8722843186038094E-4	0.0	0.0	5.369696664607902	0.0
8	3.8722843186038094E-4	0.0	0.0	6.730417374165281	0.0
9	3.8722843186038094E-4	0.0	0.0	7.429171079457338	0.0
10	3.8722843186038094E-4	0.0	0.0	8.57962675051453	0.0
11	3.8722843186038094E-4	0.0	0.0	10.02224627341038	0.0
12	3.8722843186038094E-4	0.0	0.0	11.109390095858398	0.0
13	3.8722843186038094E-4	0.0	0.0	11.705141038275594	0.0
14	3.8722843186038094E-4	0.0	0.0	11.992270920500067	0.0
15	3.8722843186038094E-4	0.0	0.0	12.353942275857662	0.0
16	3.8722843186038094E-4	0.0	0.0	12.97118439624311	0.0
17	3.8722843186038094E-4	0.0	0.0	13.636442842179244	0.0
18	3.8722843186038094E-4	0.0	0.0	14.359398324462575	0.0
19	3.8722843186038094E-4	0.0	0.0	14.83065532603666	0.0
20	3.8722843186038094E-4	0.0	0.0	15.341216013444571	0.0
21	3.8722843186038094E-4	0.0	0.0	15.89901856953945	0.0
22	3.8722843186038094E-4	0.0	0.0	16.48663771488758	0.0
23	3.8722843186038094E-4	0.0	0.0	17.053346524915245	0.0
24	3.8722843186038094E-4	0.0	0.0	17.516084500988402	0.0
25	3.8722843186038094E-4	0.0	0.0	17.978048020197836	0.0
26	3.8722843186038094E-4	0.0	0.0	18.423554331053204	0.0
27	3.8722843186038094E-4	0.0	0.0	18.892294347820194	0.0
28	3.8722843186038094E-4	0.0	0.0	19.334702831220678	0.0
29	3.8722843186038094E-4	0.0	0.0	19.80479814749918	0.0
30	3.8722843186038094E-4	0.0	0.0	20.366666602128596	0.0
31	3.8722843186038094E-4	0.0	0.0	20.86328706598953	0.0
32	3.8722843186038094E-4	0.0	0.0	21.3581650019071	0.0
33	3.8722843186038094E-4	0.0	0.0	21.84742812556269	0.0
34	3.8722843186038094E-4	0.0	0.0	22.349469787469676	0.0
35	3.8722843186038094E-4	0.0	0.0	22.879391896470608	0.0
36	3.8722843186038094E-4	0.0	0.0	23.343872400487133	0.0
37	3.8722843186038094E-4	0.0	0.0	23.799640264786802	0.0
38	3.8722843186038094E-4	0.0	0.0	24.310588180626574	0.0
39	3.8722843186038094E-4	0.0	0.0	24.984559266279568	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAA	20	7.0296763E-4	45.0	18
TAAACGG	40	6.8030204E-9	45.0	2
TACGACG	25	3.8878126E-5	45.0	1
ATAACGG	25	3.8878126E-5	45.0	2
TACGCGG	30	2.1631713E-6	44.999996	2
CGTTTTT	2505	0.0	43.29341	1
CACGACC	140	0.0	41.785713	27
ACGGGTT	70	0.0	41.785713	5
CGTTATT	260	0.0	40.67308	1
TAACGGG	150	0.0	40.500004	3
GACACGA	145	0.0	40.344826	25
ACGGGTA	45	1.9248546E-8	40.0	5
GCGTATG	45	1.9248546E-8	40.0	1
TTGTACG	40	3.4532968E-7	39.375	1
GCGATGT	80	0.0	39.375	9
ACGACCA	145	0.0	38.793102	28
CACGTGA	140	0.0	38.571426	43
ACGAATG	35	6.2416093E-6	38.571426	1
AACACGT	140	0.0	38.571426	41
CTACGAA	65	9.094947E-12	38.076927	11
>>END_MODULE