FastQCFastQC Report
Sat 14 Jan 2017
SRR2935844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205570
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC16610.8079972758671012No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC16450.8002140390134747No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC15640.7608114024419905Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG15420.7501094517682542No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC8930.43440190689302915Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT6120.2977088096512137TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.21938998881159702No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC3840.18679768448703604Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT3830.1863112321836844No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT2670.12988276499489226No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT2530.12307243274796906TruSeq Adapter, Index 27 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC2510.12209952814126573TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC2350.11431629128763925No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA2190.10653305443401274No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC2170.10556014982730944No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG2090.10166853140049618TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG800.045.0000044
TCGTTCA207.019759E-445.00000416
ACCGGGT207.019759E-445.0000044
TGCCCCG207.019759E-445.00000441
CCCTCTG207.019759E-445.00000434
CTCGTTC207.019759E-445.00000415
GGTAATC207.019759E-445.0000048
GTCGAGG302.1567794E-645.0000041
CGCGGAT302.1567794E-645.0000044
CGTGCGG302.1567794E-645.0000042
AGCTTAG207.019759E-445.0000041
ATTACGG302.1567794E-645.0000042
TGAACGC207.019759E-445.00000416
TTGTAGT207.019759E-445.00000414
CGTTCAT207.019759E-445.00000417
CGAGGTT207.019759E-445.00000436
CGGGCTT207.019759E-445.0000046
GAACGCG207.019759E-445.00000417
GGACGAT207.019759E-445.0000048
TCCCCGA207.019759E-445.00000439
Produced by FastQC (version 0.11.5)