##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	205570
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.912720727732644	33.0	31.0	34.0	30.0	34.0
2	32.011431629128765	33.0	31.0	34.0	30.0	34.0
3	31.976484895655982	33.0	31.0	34.0	30.0	34.0
4	35.63928588801868	37.0	35.0	37.0	33.0	37.0
5	31.949365179744127	37.0	35.0	37.0	0.0	37.0
6	33.739592352969794	37.0	35.0	37.0	17.0	37.0
7	35.40566230481101	37.0	35.0	37.0	32.0	37.0
8	35.60603200856156	37.0	35.0	37.0	33.0	37.0
9	37.53344846037846	39.0	37.0	39.0	35.0	39.0
10	37.18150508342657	39.0	37.0	39.0	34.0	39.0
11	37.18328549885684	39.0	37.0	39.0	34.0	39.0
12	37.02627328890402	39.0	37.0	39.0	33.0	39.0
13	36.852809262051856	39.0	35.0	39.0	33.0	39.0
14	37.97458286714988	40.0	37.0	41.0	33.0	41.0
15	38.14377097825558	40.0	37.0	41.0	33.0	41.0
16	38.162499391934624	40.0	37.0	41.0	34.0	41.0
17	38.152546577808046	40.0	37.0	41.0	34.0	41.0
18	38.108182127742374	40.0	37.0	41.0	34.0	41.0
19	37.987420343435325	40.0	36.0	41.0	34.0	41.0
20	38.01918081432115	40.0	36.0	41.0	34.0	41.0
21	37.98817434450552	40.0	36.0	41.0	34.0	41.0
22	38.04694264727343	40.0	36.0	41.0	34.0	41.0
23	38.05640900909666	40.0	36.0	41.0	34.0	41.0
24	38.01370822590845	40.0	36.0	41.0	34.0	41.0
25	37.775672520309385	40.0	36.0	41.0	33.0	41.0
26	37.81337257381914	40.0	36.0	41.0	33.0	41.0
27	37.7788539183733	40.0	36.0	41.0	33.0	41.0
28	37.728627718052245	40.0	36.0	41.0	33.0	41.0
29	37.749491657343	40.0	36.0	41.0	33.0	41.0
30	37.6301162621005	40.0	36.0	41.0	33.0	41.0
31	37.628131536702824	40.0	36.0	41.0	33.0	41.0
32	37.548966288855375	40.0	36.0	41.0	33.0	41.0
33	37.47763292309189	40.0	36.0	41.0	33.0	41.0
34	37.45154935058618	40.0	36.0	41.0	33.0	41.0
35	37.44224838254609	40.0	35.0	41.0	33.0	41.0
36	37.341372768400056	40.0	35.0	41.0	33.0	41.0
37	37.278095052780074	40.0	35.0	41.0	33.0	41.0
38	37.17025830617308	40.0	35.0	41.0	32.0	41.0
39	37.12595223038381	40.0	35.0	41.0	32.0	41.0
40	37.03333171182565	39.0	35.0	41.0	32.0	41.0
41	36.91635452643868	39.0	35.0	41.0	31.0	41.0
42	36.93459648781437	39.0	35.0	41.0	32.0	41.0
43	36.8982293136158	39.0	35.0	41.0	32.0	41.0
44	36.878085323734005	39.0	35.0	41.0	32.0	41.0
45	36.87623194045824	39.0	35.0	41.0	32.0	41.0
46	36.76501435034295	39.0	35.0	41.0	31.0	41.0
47	36.63753465972661	39.0	35.0	41.0	31.0	41.0
48	36.674183976261126	39.0	35.0	41.0	31.0	41.0
49	36.675054725884124	39.0	35.0	41.0	31.0	41.0
50	36.54820255873911	39.0	35.0	41.0	31.0	41.0
51	35.548737656272806	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	9.0
11	5.0
12	3.0
13	1.0
14	6.0
15	6.0
16	10.0
17	21.0
18	50.0
19	110.0
20	169.0
21	317.0
22	514.0
23	709.0
24	896.0
25	1179.0
26	1467.0
27	1708.0
28	1959.0
29	2231.0
30	2742.0
31	3734.0
32	5349.0
33	7774.0
34	13500.0
35	18521.0
36	14992.0
37	23349.0
38	38548.0
39	65631.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.68808678309092	21.137811937539524	22.116553971883057	11.057547307486502
2	31.797927713187725	27.15765919151627	23.593909617162037	17.450503478133967
3	31.77457800262684	26.92464853821083	25.30476236804981	15.996011091112516
4	29.115629712506692	24.599892980493262	27.959819039743156	18.324658267256897
5	24.122683270905288	35.797052099041686	21.380551636912003	18.699712993141024
6	23.83324415041105	36.14291968672472	24.302184170842047	15.721651992022181
7	75.47988519725641	6.2236707690810915	13.009680400836698	5.286763632825801
8	74.32504742909958	8.721603346791847	10.257819720776377	6.695529503332198
9	68.09845794619838	8.138347035073211	12.957143552074719	10.806051466653695
10	40.06469815634577	23.481539135087804	21.245804348883592	15.207958359682832
11	31.238507564333318	24.302184170842047	24.703993773410517	19.755314491414115
12	28.18991097922849	20.139125358758577	30.190202850610497	21.48076081140244
13	24.074038040570123	27.267597412073748	31.323636717419856	17.334727829936273
14	18.86607968088729	30.677628058568857	28.54648051758525	21.909811742958603
15	17.40429050931556	25.98530914043878	37.49817580386243	19.112224546383228
16	18.775113100160528	24.264727343483973	32.65359731478328	24.306562241572212
17	18.126185727489418	26.25529016879895	28.943425597120203	26.67509850659143
18	19.98783869241621	24.95403025733327	31.978401517731186	23.079729532519337
19	20.982633652770346	26.840978742034345	27.795884613513643	24.380502991681666
20	23.521914676265993	25.709977136741742	30.296735905044507	20.471372281947755
21	22.003210585202122	28.940506883300092	28.954127547793938	20.102154983703848
22	18.669066498029867	25.6248479836552	29.133141995427348	26.57294352288758
23	19.592352969791314	27.976358418057107	28.474972028992557	23.956316583159023
24	21.261857274894197	25.407890256360368	29.414311426764606	23.915941041980833
25	18.04202947900958	28.774140195553827	28.58296444033662	24.600865885099964
26	17.3566181835871	26.72325728462324	29.133628447730697	26.786496084058957
27	20.25490100695627	27.601303692172984	29.770394512818015	22.37340078805273
28	16.198861701610156	26.34674320182906	33.276742715376756	24.177652381184025
29	17.872744077443205	22.77861555674466	31.4681130515153	27.88052731429683
30	18.980882424478278	25.68857323539427	32.91579510628983	22.41474923383762
31	21.055601498273095	24.83144427688865	29.51257479204164	24.600379432796615
32	22.32816072384103	25.766405603930536	26.630344894683077	25.275088777545363
33	19.618134941868952	23.862917740915503	30.627523471323638	25.891423845891907
34	18.1636425548475	23.22226005740137	32.14914627620762	26.46495111154351
35	19.80590553096269	22.365617551199104	29.622513012599118	28.20596390523909
36	21.435034294887387	22.290217444179596	31.283747628545022	24.991000632387994
37	18.800408619934817	23.519968867052583	34.23699956219293	23.442622950819672
38	18.563019895899206	24.950625091209808	30.00340516612346	26.48294984676752
39	19.89103468404923	23.23588072189522	31.324123169723205	25.548961424332344
40	22.4823661040035	21.557620275332003	32.382643381816415	23.57737023884808
41	18.773653743250474	22.65554312399669	30.144962786398793	28.425840346354043
42	20.95733813299606	21.52405506640074	30.70146422143309	26.81714257917011
43	22.456584131925865	21.3221773605098	29.570462616140485	26.650775891423844
44	18.91034684049229	22.592790776864327	31.05997956900326	27.436882813640125
45	19.09568516806927	22.32280974850416	29.837524930680548	28.743980152746023
46	23.626015469183244	23.70676655153962	29.713966045629224	22.953251933647906
47	18.3849783528725	22.212871527946685	34.85625334435959	24.54589677482123
48	20.188743493700443	21.435520747190736	30.826482463394466	27.549253295714355
49	20.0291871382011	19.508196721311474	34.95986768497349	25.502748455513935
50	20.178041543026705	20.553096268910835	33.03594882521769	26.232913362844773
51	18.893807462178334	21.18743007248139	28.731818845162234	31.18694362017804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	164.0
2	243.0
3	557.0
4	871.0
5	596.0
6	321.0
7	315.0
8	309.0
9	333.0
10	357.0
11	354.0
12	351.0
13	351.0
14	351.0
15	355.5
16	360.0
17	336.0
18	312.0
19	354.5
20	397.0
21	478.0
22	559.0
23	585.0
24	611.0
25	861.0
26	1332.5
27	1554.0
28	1918.5
29	2283.0
30	2691.5
31	3100.0
32	3391.0
33	3682.0
34	4204.0
35	4726.0
36	4946.0
37	5166.0
38	5889.0
39	6612.0
40	7861.0
41	9110.0
42	10846.0
43	12582.0
44	14703.0
45	16824.0
46	21633.5
47	26443.0
48	25654.0
49	24865.0
50	24589.5
51	24314.0
52	19920.5
53	15527.0
54	13249.5
55	10972.0
56	9670.5
57	8369.0
58	7348.0
59	6327.0
60	5918.0
61	5509.0
62	4646.0
63	3783.0
64	3337.5
65	2892.0
66	2334.0
67	1776.0
68	1484.0
69	1192.0
70	954.0
71	716.0
72	573.0
73	430.0
74	374.0
75	210.5
76	103.0
77	67.5
78	32.0
79	27.0
80	22.0
81	37.5
82	53.0
83	49.5
84	46.0
85	24.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	205570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.95738677822639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10615284061053	27.749185192391884
2	11.26504248420459	8.551831492922119
3	4.238167860667188	4.826093301551783
4	2.0633354265721717	3.132752833584667
5	1.271322200720245	2.4128034246242156
6	0.9342680285534866	2.1277423748601447
7	0.6600110215432724	1.7536605535827212
8	0.5408245652257494	1.6422629761151921
9	0.469056376475413	1.6023738872403561
>10	5.340322187904497	37.98170939339398
>50	0.07048661395122327	1.7648489565598093
>100	0.033320944776941905	2.6034927275380646
>500	0.0025631495982263003	0.7321107165442429
>1k	0.005126299196452601	3.1191321690908205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	1661	0.8079972758671012	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	1645	0.8002140390134747	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1564	0.7608114024419905	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	1542	0.7501094517682542	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	893	0.43440190689302915	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	612	0.2977088096512137	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.21938998881159702	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	384	0.18679768448703604	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	383	0.1863112321836844	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	267	0.12988276499489226	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	253	0.12307243274796906	TruSeq Adapter, Index 27 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	251	0.12209952814126573	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	235	0.11431629128763925	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	219	0.10653305443401274	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	217	0.10556014982730944	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	209	0.10166853140049618	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3118159264484117	0.0
2	0.0	0.0	0.0	1.8859755800943718	0.0
3	0.0	0.0	0.0	2.4570705842292164	0.0
4	0.0	0.0	0.0	3.196478085323734	0.0
5	0.0	0.0	0.0	5.798511455951744	0.0
6	0.0	0.0	0.0	6.927567252030938	0.0
7	0.0	0.0	0.0	8.16169674563409	0.0
8	0.0	0.0	0.0	9.929950868317361	0.0
9	0.0	0.0	0.0	10.450454832903635	0.0
10	0.0	0.0	0.0	12.570413970910153	0.0
11	0.0	0.0	0.0	14.56681422386535	0.0
12	0.0	0.0	0.0	16.938269202704674	0.0
13	0.0	0.0	0.0	17.660164420878534	0.0
14	0.0	0.0	0.0	17.934523519968867	0.0
15	0.0	0.0	0.0	18.52507661623778	0.0
16	0.0	0.0	0.0	19.55343678552318	0.0
17	0.0	0.0	0.0	20.78659337451963	0.0
18	0.0	0.0	0.0	22.07666488300822	0.0
19	0.0	0.0	0.0	22.961035170501532	0.0
20	0.0	0.0	0.0	23.663472296541325	0.0
21	0.0	0.0	0.0	24.533249014934086	0.0
22	0.0	0.0	0.0	25.401566376416792	0.0
23	0.0	0.0	0.0	26.304421851437468	0.0
24	0.0	0.0	0.0	27.001021549837038	0.0
25	0.0	0.0	0.0	27.607627572116552	0.0
26	0.0	0.0	0.0	28.16802062557766	0.0
27	0.0	0.0	0.0	28.799922167631465	0.0
28	0.0	0.0	0.0	29.337451962835043	0.0
29	0.0	0.0	0.0	29.883737899498954	0.0
30	4.8645230335165636E-4	0.0	0.0	30.536070438293525	0.0
31	9.729046067033127E-4	0.0	0.0	31.10716544242837	0.0
32	0.001459356910054969	0.0	0.0	31.677773994259862	0.0
33	0.001459356910054969	0.0	0.0	32.23427542929416	0.0
34	0.001459356910054969	0.0	0.0	32.78639879359829	0.0
35	0.001459356910054969	0.0	0.0	33.39835579121467	0.0
36	0.001459356910054969	0.0	0.0	33.95874884467578	0.0
37	0.001459356910054969	0.0	0.0	34.50600768594639	0.0
38	0.001459356910054969	0.0	0.0	35.03380843508294	0.0
39	0.001459356910054969	0.0	0.0	35.59274213163399	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	80	0.0	45.000004	4
TCGTTCA	20	7.019759E-4	45.000004	16
ACCGGGT	20	7.019759E-4	45.000004	4
TGCCCCG	20	7.019759E-4	45.000004	41
CCCTCTG	20	7.019759E-4	45.000004	34
CTCGTTC	20	7.019759E-4	45.000004	15
GGTAATC	20	7.019759E-4	45.000004	8
GTCGAGG	30	2.1567794E-6	45.000004	1
CGCGGAT	30	2.1567794E-6	45.000004	4
CGTGCGG	30	2.1567794E-6	45.000004	2
AGCTTAG	20	7.019759E-4	45.000004	1
ATTACGG	30	2.1567794E-6	45.000004	2
TGAACGC	20	7.019759E-4	45.000004	16
TTGTAGT	20	7.019759E-4	45.000004	14
CGTTCAT	20	7.019759E-4	45.000004	17
CGAGGTT	20	7.019759E-4	45.000004	36
CGGGCTT	20	7.019759E-4	45.000004	6
GAACGCG	20	7.019759E-4	45.000004	17
GGACGAT	20	7.019759E-4	45.000004	8
TCCCCGA	20	7.019759E-4	45.000004	39
>>END_MODULE