##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935842.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332407
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.075169897144164	33.0	31.0	34.0	30.0	34.0
2	32.170971128766844	33.0	31.0	34.0	30.0	34.0
3	32.14121543770137	33.0	31.0	34.0	30.0	34.0
4	35.753401703333566	37.0	35.0	37.0	33.0	37.0
5	31.90831119681595	37.0	35.0	37.0	0.0	37.0
6	33.78305811851134	37.0	35.0	37.0	17.0	37.0
7	35.500455766575314	37.0	35.0	37.0	32.0	37.0
8	35.769120385551446	37.0	35.0	37.0	35.0	37.0
9	37.719019154229606	39.0	37.0	39.0	35.0	39.0
10	37.3458802010788	39.0	37.0	39.0	34.0	39.0
11	37.32163582596034	39.0	37.0	39.0	34.0	39.0
12	37.18890095575605	39.0	37.0	39.0	34.0	39.0
13	37.094594879169215	39.0	37.0	39.0	33.0	39.0
14	38.19344959642847	40.0	38.0	41.0	33.0	41.0
15	38.36045570640811	40.0	38.0	41.0	34.0	41.0
16	38.33553745859744	40.0	37.0	41.0	34.0	41.0
17	38.36164701706041	40.0	37.0	41.0	34.0	41.0
18	38.29259612463035	40.0	37.0	41.0	34.0	41.0
19	38.173895254913404	40.0	37.0	41.0	34.0	41.0
20	38.290974618464716	40.0	37.0	41.0	34.0	41.0
21	38.19529672961159	40.0	37.0	41.0	34.0	41.0
22	38.2890643097167	40.0	37.0	41.0	34.0	41.0
23	38.31030634132254	40.0	37.0	41.0	34.0	41.0
24	38.27559588095317	40.0	37.0	41.0	34.0	41.0
25	38.02500849861766	40.0	36.0	41.0	34.0	41.0
26	38.09330730098945	40.0	37.0	41.0	34.0	41.0
27	38.06552208587665	40.0	37.0	41.0	34.0	41.0
28	38.034424666147224	40.0	36.0	41.0	34.0	41.0
29	38.015282469984086	40.0	36.0	41.0	34.0	41.0
30	37.878227594485075	40.0	36.0	41.0	34.0	41.0
31	37.855045170528896	40.0	36.0	41.0	34.0	41.0
32	37.80295541309299	40.0	36.0	41.0	33.0	41.0
33	37.76584127289738	40.0	36.0	41.0	33.0	41.0
34	37.65102118788112	40.0	36.0	41.0	33.0	41.0
35	37.66046443065279	40.0	36.0	41.0	33.0	41.0
36	37.59107961023685	40.0	36.0	41.0	33.0	41.0
37	37.53599051764854	40.0	36.0	41.0	33.0	41.0
38	37.401832693054	40.0	35.0	41.0	33.0	41.0
39	37.31380807263385	40.0	35.0	41.0	33.0	41.0
40	37.210464881906816	40.0	35.0	41.0	32.0	41.0
41	37.1306079595195	39.0	35.0	41.0	32.0	41.0
42	37.16044487631127	39.0	35.0	41.0	33.0	41.0
43	37.082949516706925	39.0	35.0	41.0	32.0	41.0
44	37.030673240936565	39.0	35.0	41.0	32.0	41.0
45	36.962554940178755	39.0	35.0	41.0	32.0	41.0
46	36.85735859954814	39.0	35.0	41.0	32.0	41.0
47	36.77058545698496	39.0	35.0	41.0	32.0	41.0
48	36.756566498298774	39.0	35.0	41.0	32.0	41.0
49	36.74187065856014	39.0	35.0	41.0	32.0	41.0
50	36.61445757760817	39.0	35.0	41.0	31.0	41.0
51	35.599268366791314	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	6.0
10	11.0
11	15.0
12	6.0
13	11.0
14	10.0
15	13.0
16	31.0
17	34.0
18	66.0
19	96.0
20	208.0
21	342.0
22	589.0
23	812.0
24	1129.0
25	1558.0
26	1987.0
27	2343.0
28	2816.0
29	3448.0
30	4298.0
31	5655.0
32	7740.0
33	12103.0
34	20914.0
35	26503.0
36	25156.0
37	40262.0
38	66175.0
39	107983.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26529224715484	20.332002635323565	23.47423489878372	10.928470218737871
2	32.25804510735333	23.793722755537637	25.00127855309906	18.94695358400996
3	33.1755949784451	24.519339243758402	25.70854404389799	16.596521733898502
4	28.634776042622445	25.70974738799123	25.35265502832371	20.302821541062613
5	22.731470757234355	37.10661929502086	21.813018378072663	18.34889156967212
6	24.87643160342592	35.09974218352803	24.357489463218286	15.666336749827773
7	80.51876163859365	5.880441747616627	8.710406218882275	4.890390394907448
8	81.0040101441907	5.433098580956478	8.181837325928756	5.38105394892406
9	75.32693354833081	7.030838700749382	10.633049243848655	7.009178507071151
10	38.15172363999555	30.0183209138195	17.24392085605899	14.58603459012596
11	32.393120481818976	24.766927290941528	23.46190062182806	19.37805160541144
12	27.55898642327027	23.519360302280038	27.82372212378199	21.097931150667705
13	25.869491316368187	25.96485633575707	30.030354354751854	18.13529799312289
14	21.53685090867521	28.429605874725862	29.60136218551354	20.432181031085385
15	20.007099730150088	25.78405388574849	33.82991332914169	20.378933054959735
16	23.40323759728285	26.043675373863966	30.040883615567665	20.51220341328552
17	23.62886461476443	25.023841254847216	27.203398243719295	24.143895886669053
18	25.919730932260755	23.49439091234541	29.924460074547166	20.661418080846673
19	23.775371758115803	25.97899562885258	27.43022860529411	22.815404007737502
20	26.479887607661688	26.038260325444412	27.55567722701387	19.926174839880026
21	23.985656138408636	27.71060777901789	29.145595610200747	19.15814047237272
22	23.536808791631945	24.205266435424043	28.35860857322499	23.89931619971902
23	21.996528352291016	27.505136775098	28.591455655265985	21.906879217345
24	22.128896202546876	25.531652462192433	29.57970199183531	22.75974934342538
25	21.892138252202873	26.864356045450304	27.9509757616416	23.29252994070522
26	21.220070576131068	26.58367603570322	28.345070952176098	23.851182435989614
27	21.67192628314085	25.754571955464233	28.72683186575493	23.846669895639984
28	18.72854663108779	26.08308489291742	29.745763476701754	25.442604999293035
29	20.439701931668104	25.886037297650173	30.21988104943638	23.454379721245342
30	22.68303615748164	24.532275192760682	29.036392133739664	23.748296516018012
31	23.412262677982113	26.26870071929893	26.017803475859413	24.301233126859543
32	22.868651983863156	25.593323846970733	28.403733976721306	23.134290192444805
33	23.20167746166597	24.320185796327994	27.308690851877365	25.169445890128667
34	20.091032980653235	25.670337868937775	30.13023191449037	24.10839723591862
35	19.9493392136748	25.959140451314198	29.481328612213343	24.610191722797655
36	23.18242395617421	25.93717942161266	27.7692708035631	23.111125818650027
37	20.88885011446811	26.547876548929477	27.93503145240624	24.628241884196182
38	21.066042532196974	27.130295090055263	27.160980364432756	24.642682013315003
39	22.069631505955048	24.399004834434894	28.902820939390566	24.62854272021949
40	24.15653099964802	24.146603410878832	28.502107356343277	23.194758233129868
41	19.809450462836224	25.435986606780244	27.945861549245354	26.808701381138185
42	21.336193281128253	23.330435279642124	29.981919754999144	25.351451684230476
43	21.913497609857796	23.422791938797918	29.289094393319033	25.37461605802525
44	22.245018907544065	23.831327258451235	28.684113150445086	25.239540683559614
45	21.58528550842792	23.575616638638778	28.209093069640527	26.630004783292772
46	22.832551661066102	24.547016157902814	27.643521345820034	24.97691083521105
47	19.45145559509878	25.300911232314604	31.173832079348507	24.07380109323811
48	20.091032980653235	24.88846504435827	29.24126146561294	25.779240509375555
49	23.09849070567106	22.519381360801667	30.57968093331368	23.802447000213593
50	20.427066818689138	24.581612300583323	29.468392663211063	25.522928217516476
51	20.517618461705077	23.033209288613055	27.793638521451115	28.655533728230754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	261.5
2	438.0
3	776.0
4	1114.0
5	806.5
6	499.0
7	462.5
8	426.0
9	456.5
10	487.0
11	509.5
12	532.0
13	543.0
14	554.0
15	541.0
16	528.0
17	566.0
18	604.0
19	634.5
20	665.0
21	749.0
22	833.0
23	875.0
24	917.0
25	1397.5
26	2163.0
27	2448.0
28	3093.5
29	3739.0
30	4284.5
31	4830.0
32	5779.5
33	6729.0
34	7408.5
35	8088.0
36	8898.0
37	9708.0
38	10580.0
39	11452.0
40	13209.0
41	14966.0
42	16449.0
43	17932.0
44	20150.5
45	22369.0
46	26163.5
47	29958.0
48	32002.5
49	34047.0
50	34576.0
51	35105.0
52	31126.5
53	27148.0
54	24758.0
55	22368.0
56	19872.5
57	17377.0
58	15903.0
59	14429.0
60	12932.5
61	11436.0
62	10123.5
63	8811.0
64	7952.0
65	7093.0
66	6012.0
67	4931.0
68	4032.0
69	3133.0
70	2517.5
71	1902.0
72	1570.0
73	1238.0
74	969.0
75	604.0
76	508.0
77	388.0
78	268.0
79	184.5
80	101.0
81	57.0
82	13.0
83	9.5
84	6.0
85	6.5
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	332407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.59235414207686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15112003896391	26.401793362132654
2	10.427316191147275	7.63120093635746
3	4.127810431248751	4.531389033948397
4	2.1420613960165378	3.135322767884347
5	1.4190433961956006	2.596306924828245
6	1.0441075746565163	2.292381248055371
7	0.7755520039383312	1.9865491508596158
8	0.6805503350810719	1.9922351098236522
9	0.6161252516430311	2.0290926063598214
>10	6.518856766385903	43.27150844988953
>50	0.07598312334342736	1.905783800525344
>100	0.01899578083585684	1.4600129305959304
>500	0.0016518070292049422	0.3925805830768192
>1k	8.259035146024711E-4	0.3738430956628371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1237	0.37213416083295475	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	797	0.23976631057709374	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	502	0.1510196837010051	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	493	0.14831215949122611	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	486	0.1462063073280647	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	451	0.13567704651225757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	378	0.11371601681071698	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	343	0.10318675599490985	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018050161398526505	0.0	0.0	0.1582397482604157	0.0
2	0.0018050161398526505	0.0	0.0	0.9747087155204313	0.0
3	0.0018050161398526505	0.0	0.0	1.3086367013931717	0.0
4	0.0018050161398526505	0.0	0.0	1.7367263625615585	0.0
5	0.0018050161398526505	0.0	0.0	3.014677789577235	0.0
6	0.0018050161398526505	0.0	0.0	3.7791021248048327	0.0
7	0.0018050161398526505	0.0	0.0	4.534200543309858	0.0
8	0.0018050161398526505	0.0	0.0	5.760709010339734	0.0
9	0.0018050161398526505	0.0	0.0	6.197823752207384	0.0
10	0.0018050161398526505	0.0	0.0	7.3106162024265435	0.0
11	0.0018050161398526505	0.0	0.0	8.712211235022126	0.0
12	0.0018050161398526505	0.0	0.0	9.958574879590381	0.0
13	0.0018050161398526505	0.0	0.0	10.490152132776988	0.0
14	0.0018050161398526505	0.0	0.0	10.677572975298354	0.0
15	0.0018050161398526505	0.0	0.0	11.060537232970425	0.0
16	0.0018050161398526505	0.0	0.0	11.838499189246917	0.0
17	0.0018050161398526505	0.0	0.0	12.724160441867951	0.0
18	0.0018050161398526505	0.0	0.0	13.705487549901175	0.0
19	0.0018050161398526505	0.0	0.0	14.271059273721672	0.0
20	0.0018050161398526505	0.0	0.0	14.83392347333239	0.0
21	0.0018050161398526505	0.0	0.0	15.51441455805684	0.0
22	0.0018050161398526505	0.0	0.0	16.22137921283246	0.0
23	0.0018050161398526505	0.0	0.0	16.9391739644472	0.0
24	0.0021058521631614254	0.0	0.0	17.48669552686917	0.0
25	0.0021058521631614254	0.0	0.0	17.986083325561737	0.0
26	0.0021058521631614254	0.0	0.0	18.477950223671584	0.0
27	0.0021058521631614254	0.0	0.0	18.985761431016794	0.0
28	0.0021058521631614254	0.0	0.0	19.496881834618403	0.0
29	0.0021058521631614254	0.0	0.0	19.9869437165884	0.0
30	0.0021058521631614254	0.0	0.0	20.61809769349021	0.0
31	0.0024066881864702005	0.0	0.0	21.11868883627601	0.0
32	0.0024066881864702005	0.0	0.0	21.640940172740045	0.0
33	0.0024066881864702005	0.0	0.0	22.144238839735625	0.0
34	0.0024066881864702005	0.0	0.0	22.643325802404885	0.0
35	0.0024066881864702005	0.0	0.0	23.227248523647216	0.0
36	0.0024066881864702005	0.0	0.0	23.698357736148758	0.0
37	0.0024066881864702005	0.0	0.0	24.214893188169924	0.0
38	0.0024066881864702005	0.0	0.0	24.73714452463396	0.0
39	0.0024066881864702005	0.0	0.0	25.46155766876149	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.1608303E-6	45.000004	2
TCACGAC	25	3.884803E-5	45.0	25
CTCACGT	20	7.026045E-4	45.0	45
CTCACGA	25	3.884803E-5	45.0	24
TCGCACG	20	7.026045E-4	45.0	13
TCACCCG	20	7.026045E-4	45.0	26
TACTCGC	20	7.026045E-4	45.0	45
TCTCCGT	20	7.026045E-4	45.0	41
CATCGGG	20	7.026045E-4	45.0	3
GCGGATG	20	7.026045E-4	45.0	1
CGGTCTA	25	3.884803E-5	45.0	31
GCGCTAG	20	7.026045E-4	45.0	1
GCTCACG	20	7.026045E-4	45.0	44
CGGACGG	45	3.8380676E-10	45.0	2
CGCACGG	65	0.0	41.538464	2
TAGACGG	50	1.0768417E-9	40.5	2
ACGGGCG	50	1.0768417E-9	40.5	5
CGTTAGG	45	1.9212166E-8	40.0	2
AAGGGCG	135	0.0	40.0	5
GCCAGTA	45	1.9212166E-8	40.0	25
>>END_MODULE