FastQCFastQC Report
Sat 14 Jan 2017
SRR2935841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662341
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52960.7995881275657101No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT16740.2527399028597052No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC16340.24670071760618775No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC14570.21997732285937302No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG13130.1982362559467102TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC12180.1838931909696063TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC10600.16003840921821239TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT8800.13286207557738383No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC8130.12274644027774213No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG8080.12199154212105245No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT7710.1164052957615488TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT7420.11202688645274865TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG351.210883E-745.01
CACGACG351.210883E-745.026
TATCGCC207.031119E-445.042
GTACGAG351.210883E-745.01
CGAACGG502.1827873E-1144.9999962
GCGATTA253.8890103E-544.9999969
CGTACTC253.8890103E-544.99999631
CGTTATT4000.042.7499961
CGTTTTT29500.042.1016961
CGTTCTG1900.039.078951
TGCGATG356.2442814E-638.5714261
TGCACGG1650.038.1818162
AACGAGC4700.037.8191515
GGTAATC301.13939954E-437.5000048
CGTATGG301.13939954E-437.5000042
TGTTACG301.13939954E-437.5000041
GCTAACG601.546141E-1037.5000041
CGATCGG301.13939954E-437.5000042
CGCACGG1150.037.1739122
ACAACGA4700.036.86170213
Produced by FastQC (version 0.11.5)