##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935841.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662341
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.125009624951495	33.0	31.0	34.0	30.0	34.0
2	32.2532577629952	34.0	31.0	34.0	30.0	34.0
3	32.163737410185995	34.0	31.0	34.0	30.0	34.0
4	35.809053040654284	37.0	35.0	37.0	35.0	37.0
5	32.185357693393584	37.0	35.0	37.0	0.0	37.0
6	33.94241636860771	37.0	35.0	37.0	17.0	37.0
7	35.5767738974335	37.0	35.0	37.0	32.0	37.0
8	35.824489198162276	37.0	35.0	37.0	35.0	37.0
9	37.77709971147792	39.0	38.0	39.0	35.0	39.0
10	37.39796419065104	39.0	37.0	39.0	34.0	39.0
11	37.39406589656989	39.0	37.0	39.0	35.0	39.0
12	37.36707979726455	39.0	37.0	39.0	35.0	39.0
13	37.35547701259623	39.0	37.0	39.0	34.0	39.0
14	38.58487697424741	40.0	38.0	41.0	35.0	41.0
15	38.69865371462736	40.0	38.0	41.0	35.0	41.0
16	38.627353583728016	40.0	38.0	41.0	35.0	41.0
17	38.61130595871311	40.0	38.0	41.0	35.0	41.0
18	38.48759173899849	40.0	38.0	41.0	34.0	41.0
19	38.30568694977361	40.0	37.0	41.0	34.0	41.0
20	38.36146335497878	40.0	37.0	41.0	34.0	41.0
21	38.258267267162985	40.0	37.0	41.0	34.0	41.0
22	38.334271923374814	40.0	37.0	41.0	34.0	41.0
23	38.34349526905325	40.0	37.0	41.0	34.0	41.0
24	38.31695154006773	40.0	37.0	41.0	34.0	41.0
25	38.05166522984384	40.0	36.0	41.0	34.0	41.0
26	38.11847975589613	40.0	37.0	41.0	34.0	41.0
27	38.10535811613655	40.0	37.0	41.0	34.0	41.0
28	38.03261009057268	40.0	36.0	41.0	34.0	41.0
29	38.001707579630434	40.0	36.0	41.0	34.0	41.0
30	37.8197861826461	40.0	36.0	41.0	33.0	41.0
31	37.764174345239084	40.0	36.0	41.0	33.0	41.0
32	37.61297277384308	40.0	36.0	41.0	33.0	41.0
33	37.47335586955964	40.0	36.0	41.0	33.0	41.0
34	37.30239559381044	40.0	36.0	41.0	33.0	41.0
35	37.240996707134244	40.0	36.0	41.0	32.0	41.0
36	37.13518565210367	40.0	36.0	41.0	32.0	41.0
37	37.08843329946357	40.0	36.0	41.0	32.0	41.0
38	36.952541062685235	40.0	35.0	41.0	31.0	41.0
39	36.860547663514716	40.0	35.0	41.0	31.0	41.0
40	36.76902985018291	40.0	35.0	41.0	31.0	41.0
41	36.63610134356774	39.0	35.0	41.0	31.0	41.0
42	36.629110986636796	39.0	35.0	41.0	31.0	41.0
43	36.55286174342219	39.0	35.0	41.0	31.0	41.0
44	36.49109295664922	39.0	35.0	41.0	31.0	41.0
45	36.42578369752137	39.0	35.0	41.0	31.0	41.0
46	36.28623322427571	39.0	35.0	41.0	30.0	41.0
47	36.20893920201225	39.0	35.0	41.0	30.0	41.0
48	36.157447598744454	39.0	35.0	41.0	30.0	41.0
49	36.11569720129057	39.0	35.0	41.0	30.0	41.0
50	36.013905224046226	39.0	35.0	41.0	30.0	41.0
51	35.0307726684593	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	30.0
10	34.0
11	19.0
12	14.0
13	17.0
14	21.0
15	35.0
16	51.0
17	78.0
18	137.0
19	286.0
20	511.0
21	774.0
22	1263.0
23	2048.0
24	3197.0
25	5065.0
26	6781.0
27	7769.0
28	7870.0
29	7893.0
30	9238.0
31	11397.0
32	14415.0
33	20992.0
34	35252.0
35	45984.0
36	50098.0
37	78708.0
38	132521.0
39	219647.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.939300149016894	19.05725298599966	21.863058454783864	15.140388410199579
2	35.40110003759393	22.980458706315932	23.073764118482774	18.544677137607366
3	31.53330384197868	23.746982294618633	27.974864910974862	16.74484895242783
4	27.136927957049316	24.726538142739162	28.381302078536585	19.755231821674936
5	22.28338574842868	35.788966710501086	24.423220063381248	17.504427477688985
6	24.400875077943233	33.209630688723784	26.757516143497078	15.631978089835899
7	78.64045861572815	5.208797281158799	11.588743562605968	4.56200054050708
8	79.53259725730402	4.939751578114596	11.059107015872488	4.468544148708898
9	73.365381276412	6.823524438318026	13.159686626677194	6.65140765859278
10	35.13733862164655	30.073330806940834	19.767310192181974	15.02202037923064
11	28.073454610238535	25.28788041205361	27.776779634659487	18.86188534304837
12	23.708633468258796	23.821566232499574	31.731087159031375	20.738713140210255
13	23.471595447058238	24.01195154761671	33.91002519850047	18.606427806824584
14	20.509526059839267	26.774274882575593	32.1068754614315	20.609323596153644
15	19.299424314665707	25.43357575629472	34.64710775869227	20.6198921703473
16	22.967927396914884	26.784692477137913	30.864917014045634	19.382463111901576
17	23.043115253321176	26.019980644411262	29.96553135016555	20.971372752102013
18	24.921301867165102	24.223775970383834	30.349472552657918	20.505449609793143
19	22.621127183731645	26.623748190131668	28.81989790757329	21.9352267185634
20	24.727745979789866	26.592193447182037	29.074751525271726	19.605309047756368
21	24.067964990843084	27.12681232174967	30.06834847910668	18.73687420830056
22	23.879391431302004	23.382668444200196	30.46512295026278	22.272817174235023
23	21.570459929250944	27.158669023961977	30.427076083165623	20.843794963621455
24	21.68943187874524	24.802178938039468	31.523641145573052	21.98474803764224
25	21.78938039469095	27.378344387558673	29.399508712279626	21.432766505470745
26	22.016151800960532	25.60659841380799	30.055817169705634	22.32143261552584
27	22.463655428246177	24.792818200896516	30.525967741691968	22.21755862916534
28	19.570432752917306	26.440458917687415	30.450326946391666	23.538781383003617
29	21.01259019145727	25.486720586525674	30.59481445358207	22.90587476843499
30	21.688827960219886	23.550708773879318	32.51754005867068	22.24292320723011
31	22.461390733776106	24.61541713407444	28.956685453565463	23.966506678583993
32	21.382490288235214	24.995583845783365	29.951339264819783	23.670586601161638
33	20.511035856152645	24.369924253518956	28.979483377897488	26.139556512430907
34	19.412960997431835	24.745108637393727	31.2301367422521	24.61179362292233
35	19.388955236049103	25.63573748265621	30.757268536901687	24.218038744392995
36	20.368360104538297	27.951765027380155	28.686733872733228	22.99314099534832
37	20.60147265532407	26.09984886938903	28.634494920290305	24.664183554996598
38	19.771386642228098	27.460477307006514	28.286788829319036	24.481347221446352
39	20.89195746601826	25.69114700735724	27.47587722940298	25.941018297221525
40	22.825855563825883	23.65956508807397	30.402919342151552	23.111660005948597
41	19.88824487688366	25.864018685239177	29.358744211818383	24.888992226058782
42	21.493007378374585	25.88032448542367	30.050985821502824	22.575682314698923
43	21.750427649805765	24.665391392047297	29.68606805255903	23.898112905587908
44	22.101153333403794	24.44299839508652	28.505709294759047	24.950138976750647
45	22.07548679607634	23.30023356548968	28.664539866926553	25.95973977150743
46	22.11217484649146	24.40404565020133	28.103952495768798	25.379827007538413
47	20.514810346936095	25.229602274357166	30.75757049616436	23.498016882542377
48	20.742185671731026	25.18385544606177	29.956472572285275	24.11748630992193
49	22.321734574788515	23.551614651667343	30.228537867956234	23.898112905587908
50	19.830268698449892	24.43469451536293	31.09183939994655	24.643197386240622
51	20.429506855230162	22.808190946959346	29.270572107117026	27.491730090693466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	517.0
2	827.0
3	5095.0
4	9363.0
5	6075.0
6	2787.0
7	2712.0
8	2637.0
9	2630.0
10	2623.0
11	2610.5
12	2598.0
13	2600.5
14	2603.0
15	2524.0
16	2445.0
17	2415.0
18	2385.0
19	2464.0
20	2543.0
21	2548.5
22	2554.0
23	2821.0
24	3088.0
25	3441.0
26	4615.5
27	5437.0
28	6383.5
29	7330.0
30	8626.0
31	9922.0
32	11376.0
33	12830.0
34	15081.0
35	17332.0
36	18179.5
37	19027.0
38	20899.0
39	22771.0
40	25073.0
41	27375.0
42	30542.5
43	33710.0
44	37447.5
45	41185.0
46	49789.0
47	58393.0
48	61193.5
49	63994.0
50	64239.5
51	64485.0
52	58424.0
53	52363.0
54	48300.5
55	44238.0
56	39886.0
57	35534.0
58	32042.0
59	28550.0
60	25141.0
61	21732.0
62	19864.5
63	17997.0
64	15416.0
65	12835.0
66	11013.0
67	9191.0
68	7611.5
69	6032.0
70	4846.5
71	3661.0
72	3197.5
73	2734.0
74	2226.5
75	1321.0
76	923.0
77	628.5
78	334.0
79	240.5
80	147.0
81	112.0
82	77.0
83	45.5
84	14.0
85	10.5
86	7.0
87	6.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	662341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.015901773310155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3276563592846	26.402575215157004
2	9.609126887179405	7.113809939662128
3	4.001795230985481	4.443901775611788
4	2.3926368827572624	3.5426244732536736
5	1.7505727299401967	3.2399514109250864
6	1.390587772427387	3.0884316234802998
7	1.2067519888036335	3.1268309157611385
8	1.0416220596331875	3.084526387543607
9	0.9489722028038775	3.1614355660131084
>10	6.250361991634125	36.871164911805856
>50	0.04306348731498345	1.099423514655772
>100	0.031469471347482375	2.01967149021035
>500	0.00248443194848545	0.7131768569438232
>1k	0.00248443194848545	1.2807448563555957
>5k	4.140719914142417E-4	0.8117310626207797
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5296	0.7995881275657101	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1674	0.2527399028597052	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1634	0.24670071760618775	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	1457	0.21997732285937302	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTG	1313	0.1982362559467102	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1218	0.1838931909696063	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	1060	0.16003840921821239	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	880	0.13286207557738383	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	813	0.12274644027774213	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	808	0.12199154212105245	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	771	0.1164052957615488	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	742	0.11202688645274865	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.019592626758724E-4	0.0	0.0	0.11746215318091437	0.0
2	3.019592626758724E-4	0.0	0.0	0.5527364303281844	0.0
3	3.019592626758724E-4	0.0	0.0	0.9162953825899348	0.0
4	3.019592626758724E-4	0.0	0.0	1.3494559448984738	0.0
5	3.019592626758724E-4	0.0	0.0	2.6184397462938276	0.0
6	3.019592626758724E-4	0.0	0.0	3.8664373789332083	0.0
7	3.019592626758724E-4	0.0	0.0	4.78077002631575	0.0
8	3.019592626758724E-4	0.0	0.0	6.0423558257755445	0.0
9	3.019592626758724E-4	0.0	0.0	6.658352721634325	0.0
10	3.019592626758724E-4	0.0	0.0	7.7745451361156865	0.0
11	3.019592626758724E-4	0.0	0.0	8.829741779536523	0.0
12	3.019592626758724E-4	0.0	0.0	9.820319140744722	0.0
13	3.019592626758724E-4	0.0	0.0	10.259216929044102	0.0
14	3.019592626758724E-4	0.0	0.0	10.488705968677765	0.0
15	3.019592626758724E-4	0.0	0.0	10.786437801676175	0.0
16	3.019592626758724E-4	0.0	0.0	11.287841157349462	0.0
17	3.019592626758724E-4	0.0	0.0	11.880587189982199	0.0
18	3.019592626758724E-4	0.0	0.0	12.503982087776539	0.0
19	3.019592626758724E-4	0.0	0.0	12.91645844059178	0.0
20	3.019592626758724E-4	0.0	0.0	13.322593648890829	0.0
21	3.019592626758724E-4	0.0	0.0	13.793046180139838	0.0
22	3.019592626758724E-4	0.0	0.0	14.332647382541621	0.0
23	3.019592626758724E-4	0.0	0.0	14.848846742086025	0.0
24	3.019592626758724E-4	0.0	0.0	15.262832891214646	0.0
25	3.019592626758724E-4	0.0	0.0	15.646925073338355	0.0
26	3.019592626758724E-4	0.0	0.0	16.003840921821237	0.0
27	4.529388940138086E-4	0.0	0.0	16.40016245408332	0.0
28	4.529388940138086E-4	0.0	0.0	16.76885471381056	0.0
29	4.529388940138086E-4	0.0	0.0	17.176952657317003	0.0
30	4.529388940138086E-4	0.0	0.0	17.67020311289804	0.0
31	6.039185253517448E-4	0.0	0.0	18.10759110488404	0.0
32	6.039185253517448E-4	0.0	0.0	18.554490813644332	0.0
33	6.039185253517448E-4	0.0	0.0	18.990369009316954	0.0
34	6.039185253517448E-4	0.0	0.0	19.417641366003313	0.0
35	6.039185253517448E-4	0.0	0.0	19.882054711998805	0.0
36	6.039185253517448E-4	0.0	0.0	20.285623266565107	0.0
37	6.039185253517448E-4	0.0	0.0	20.675452674679658	0.0
38	6.039185253517448E-4	0.0	0.0	21.132166059476916	0.0
39	6.039185253517448E-4	0.0	0.0	21.663312402523776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACG	35	1.210883E-7	45.0	1
CACGACG	35	1.210883E-7	45.0	26
TATCGCC	20	7.031119E-4	45.0	42
GTACGAG	35	1.210883E-7	45.0	1
CGAACGG	50	2.1827873E-11	44.999996	2
GCGATTA	25	3.8890103E-5	44.999996	9
CGTACTC	25	3.8890103E-5	44.999996	31
CGTTATT	400	0.0	42.749996	1
CGTTTTT	2950	0.0	42.101696	1
CGTTCTG	190	0.0	39.07895	1
TGCGATG	35	6.2442814E-6	38.571426	1
TGCACGG	165	0.0	38.181816	2
AACGAGC	470	0.0	37.81915	15
GGTAATC	30	1.13939954E-4	37.500004	8
CGTATGG	30	1.13939954E-4	37.500004	2
TGTTACG	30	1.13939954E-4	37.500004	1
GCTAACG	60	1.546141E-10	37.500004	1
CGATCGG	30	1.13939954E-4	37.500004	2
CGCACGG	115	0.0	37.173912	2
ACAACGA	470	0.0	36.861702	13
>>END_MODULE