SRR2935840.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935840.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703271
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12330	1.753235950295121	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	9055	1.2875548686068388	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	3206	0.4558697856160712	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	3107	0.44179270864289866	No Hit
GAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	3065	0.4358206153815528	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2316	0.3293182855542173	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	2263	0.3217820726291856	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	1846	0.26248771810582266	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	1753	0.2492637973128424	No Hit
CGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	1622	0.23063655404531114	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	1477	0.21001861302399785	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.19736346301781246	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	1146	0.16295283041672415	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	979	0.1392066500680392	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTC	976	0.13878007197794306	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	973	0.13835349388784693	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	931	0.13238140062650103	TruSeq Adapter, Index 19 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	917	0.13039070287271906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	760	0.10806644949102125	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTC	730	0.10380066859005989	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGG	35	1.2110286E-7	45.000004	2
TAGCACG	20	7.031416E-4	45.0	1
CGTAGTA	20	7.031416E-4	45.0	10
GCGTAAG	40	6.8084773E-9	45.0	1
CGCCCGT	20	7.031416E-4	45.0	38
CGTTTTT	7255	0.0	44.007584	1
CGTTATT	815	0.0	43.343555	1
TGCGAAG	70	0.0	41.785717	1
ACGGGTC	50	1.0804797E-9	40.5	5
CCCGTCG	50	1.0804797E-9	40.5	40
CGTTTCT	235	0.0	39.255318	1
TGGGCGA	315	0.0	38.57143	6
CCGATGA	455	0.0	38.076923	18
CCGTCGA	65	9.094947E-12	38.07692	41
CGCGACC	60	1.546141E-10	37.500004	10
CGTTACT	30	1.1394713E-4	37.500004	1
TTAACGG	60	1.546141E-10	37.500004	2
CGATGAT	30	1.1394713E-4	37.500004	10
GCCCGTC	30	1.1394713E-4	37.500004	39
GTTTTTT	8605	0.0	37.391052	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content