##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935840.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703271
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993370976479905	33.0	31.0	34.0	30.0	34.0
2	32.141021028877915	33.0	31.0	34.0	30.0	34.0
3	31.965610696303415	33.0	31.0	34.0	30.0	34.0
4	35.694848785176696	37.0	35.0	37.0	33.0	37.0
5	31.864722134141747	37.0	35.0	37.0	0.0	37.0
6	33.73237485976245	37.0	35.0	37.0	17.0	37.0
7	35.460801028337585	37.0	35.0	37.0	32.0	37.0
8	35.709870021655945	37.0	35.0	37.0	35.0	37.0
9	37.69679398126753	39.0	37.0	39.0	35.0	39.0
10	37.377805995128476	39.0	37.0	39.0	34.0	39.0
11	37.34576571478136	39.0	37.0	39.0	34.0	39.0
12	37.39904105245346	39.0	37.0	39.0	35.0	39.0
13	37.399614089021156	39.0	37.0	39.0	34.0	39.0
14	38.673795734503486	40.0	38.0	41.0	35.0	41.0
15	38.75294018948599	40.0	38.0	41.0	35.0	41.0
16	38.70540659290658	40.0	38.0	41.0	35.0	41.0
17	38.6715206513563	40.0	38.0	41.0	34.0	41.0
18	38.432281439160725	40.0	38.0	41.0	34.0	41.0
19	38.16946241207159	40.0	37.0	41.0	34.0	41.0
20	38.03909019424944	40.0	36.0	41.0	34.0	41.0
21	38.00456438556403	40.0	36.0	41.0	34.0	41.0
22	38.066816348178726	40.0	36.0	41.0	34.0	41.0
23	38.0660982750604	40.0	36.0	41.0	34.0	41.0
24	38.01686547575543	40.0	35.0	41.0	34.0	41.0
25	37.79607576595651	40.0	35.0	41.0	34.0	41.0
26	37.79700144041202	40.0	35.0	41.0	34.0	41.0
27	37.78888081550355	40.0	35.0	41.0	34.0	41.0
28	37.705430765665014	40.0	35.0	41.0	33.0	41.0
29	37.6199075463086	40.0	35.0	41.0	33.0	41.0
30	37.37874020114579	40.0	35.0	41.0	33.0	41.0
31	37.21792879274135	40.0	35.0	41.0	33.0	41.0
32	36.87649284557447	40.0	35.0	41.0	31.0	41.0
33	36.47461647074883	40.0	35.0	41.0	30.0	41.0
34	36.122951180981445	40.0	35.0	41.0	27.0	41.0
35	35.95640087533824	40.0	35.0	41.0	25.0	41.0
36	35.780505665668	40.0	35.0	41.0	24.0	41.0
37	35.693765276827854	40.0	35.0	41.0	23.0	41.0
38	35.55896802228444	40.0	35.0	41.0	23.0	41.0
39	35.45744101491459	39.0	35.0	41.0	22.0	41.0
40	35.367870991410136	39.0	35.0	41.0	22.0	41.0
41	35.26235263504396	39.0	35.0	41.0	21.0	41.0
42	35.250871996712505	39.0	35.0	41.0	21.0	41.0
43	35.188221894547056	39.0	35.0	41.0	21.0	41.0
44	35.15029625848357	39.0	35.0	41.0	20.0	41.0
45	35.16171148817455	39.0	35.0	41.0	20.0	41.0
46	35.043569264195455	39.0	35.0	41.0	20.0	41.0
47	34.940971545819465	39.0	35.0	41.0	20.0	41.0
48	34.90761313917395	39.0	35.0	41.0	20.0	41.0
49	34.896650082258475	39.0	35.0	41.0	20.0	41.0
50	34.795639234377646	39.0	35.0	41.0	20.0	41.0
51	33.85230728979298	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	14.0
10	13.0
11	15.0
12	9.0
13	14.0
14	21.0
15	23.0
16	46.0
17	107.0
18	226.0
19	479.0
20	981.0
21	1664.0
22	2683.0
23	4029.0
24	6285.0
25	10399.0
26	14401.0
27	15339.0
28	14013.0
29	12199.0
30	11801.0
31	13537.0
32	16279.0
33	21930.0
34	33523.0
35	42707.0
36	49352.0
37	74717.0
38	128787.0
39	227491.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51979962205181	17.866796725586582	21.083906488394945	20.529497163966663
2	39.600808223288034	24.387327218099426	21.30387859018785	14.707985968424689
3	27.609982496079038	24.713801649719667	33.469032563549476	14.207183290651827
4	24.05345876625085	22.05465602875705	37.13319616477859	16.75868904021352
5	21.71154505162306	33.60283589114296	28.585140010038806	16.10047904719518
6	21.971331108491608	31.416196601310165	32.681000638445205	13.931471651753021
7	71.01999087122888	4.04779949692224	20.613817433109	4.318392198739889
8	71.70351116425958	5.46972646390936	18.23905720554381	4.587705166287249
9	65.51315211348114	6.077742434993054	19.851948964197298	8.557156487328497
10	30.75286767121067	29.85420982807481	26.147246225139387	13.245676275575132
11	22.65826402624308	21.667038737556364	37.31548720194633	18.359210034254218
12	20.14785196602732	20.30156227115863	39.43501153893734	20.115574223876713
13	21.070682567601963	20.776770263525723	41.548279397273596	16.604267771598717
14	17.806933600276423	25.63563690241742	38.1252746096455	18.43215488766066
15	16.848697017223802	25.02904285830071	40.69128401427046	17.430976110205027
16	19.884511091741306	25.476238889418163	36.15519479688484	18.48405522195569
17	19.863893150719996	25.583452182728987	35.82303265739665	18.729622009154365
18	19.874841988365795	24.386331869222534	36.52845062571896	19.210375516692714
19	18.665919681033344	26.46277181911383	34.50476416630289	20.36654433354994
20	19.729947630429805	26.76720638274577	35.59595092076881	17.90689506605562
21	19.7418918169525	26.895748580561406	36.71557621457447	16.64678338791163
22	19.25004727907165	22.218319822657268	37.65362143469587	20.87801146357521
23	17.96078609810443	25.230814294916183	37.45839086212854	19.350008744850847
24	19.048986805939673	24.67398769464403	36.48181142120178	19.795214078214514
25	19.118376841928644	27.997599787279725	33.82750035192693	19.056523018864706
26	18.496852564658575	25.599519957455946	35.12131169918851	20.782315778696976
27	20.455130383593236	24.33684881077138	34.99931036542101	20.208710440214368
28	18.464574822507966	25.070278740343337	35.654960889898774	20.810185547249922
29	19.48452303592783	25.47751862368845	35.00130106317479	20.036657277208928
30	20.031822725521174	25.14222824487289	36.30549247729538	18.52045655231056
31	22.31728593955957	24.040377038154567	33.660708318699335	19.981628703586527
32	23.470753095179525	25.417655498378295	33.08980464145401	18.021786764988175
33	21.707137078025397	25.94902960594138	32.137824537056126	20.206008778977093
34	20.71207258652781	25.961684755947566	32.47780727486275	20.848435382661876
35	20.61609251625618	25.638196370957996	33.286314948291626	20.459396164494198
36	22.48578428514755	27.164919355412064	31.4781926170708	18.871103742369584
37	21.87890585563744	25.197825589282086	32.80229100872921	20.120977546351266
38	21.28411380534673	26.47215653709594	31.477197268193912	20.76653238936342
39	22.772160376298753	25.03586810774225	30.63811816497481	21.553853350984188
40	21.193537057549648	25.472684072000696	33.47884385962168	19.854935010827973
41	20.211269908754947	26.244221644287908	31.690344120545284	21.85416432641187
42	21.077081238953404	25.514915302920212	33.59515748552123	19.812845972605157
43	22.771733798208658	25.126018277449237	31.3792265001685	20.723021424173613
44	21.816483261786708	24.995769767273213	31.346522179927796	21.841224791012284
45	20.86564069896242	23.952359758898066	31.015781967406593	24.166217574732926
46	22.713150407168786	25.278733233703647	30.5070164986186	21.501099860508965
47	20.001820066517745	24.901638202058667	33.9295947081566	21.166947023266992
48	19.95461209121377	24.941452157134307	32.22541523822253	22.87852051342939
49	20.782884549483768	23.39752385637969	34.185683754910976	21.63390783922556
50	19.625435998356252	23.822111248722045	34.34650369487722	22.20594905804448
51	19.112689134060695	23.106597598934123	32.819211939636354	24.961501327368822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	390.5
2	619.0
3	11640.5
4	22662.0
5	15126.0
6	7590.0
7	7339.0
8	7088.0
9	6997.0
10	6906.0
11	6790.5
12	6675.0
13	6474.5
14	6274.0
15	5942.0
16	5610.0
17	5297.5
18	4985.0
19	4827.0
20	4669.0
21	4630.0
22	4591.0
23	4647.5
24	4704.0
25	5133.5
26	6250.0
27	6937.0
28	7988.5
29	9040.0
30	10535.0
31	12030.0
32	13899.0
33	15768.0
34	17504.5
35	19241.0
36	21303.0
37	23365.0
38	25104.0
39	26843.0
40	28906.5
41	30970.0
42	33706.5
43	36443.0
44	41431.5
45	46420.0
46	64638.5
47	82857.0
48	75852.5
49	68848.0
50	66300.5
51	63753.0
52	54944.5
53	46136.0
54	40833.0
55	35530.0
56	31504.5
57	27479.0
58	24179.0
59	20879.0
60	18205.5
61	15532.0
62	13093.0
63	10654.0
64	8874.0
65	7094.0
66	5488.0
67	3882.0
68	3089.0
69	2296.0
70	1762.0
71	1228.0
72	1076.5
73	925.0
74	706.0
75	390.5
76	294.0
77	192.0
78	90.0
79	89.0
80	88.0
81	63.0
82	38.0
83	28.5
84	19.0
85	11.5
86	4.0
87	2.0
88	0.0
89	1.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.86933972231415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76523957422941	32.36032354579763
2	9.825489748633705	8.620754954418544
3	4.402452182677386	5.79398011239353
4	2.6570791186576215	4.66257226101833
5	1.938482386671842	4.251997118331468
6	1.4031112887674049	3.6932139477090793
7	1.0753463691214076	3.3022314630300844
8	0.881028000584212	3.092009332999999
9	0.6912631616145052	2.7292732626948917
>10	3.302948377137242	21.653463853752527
>50	0.03270443194164559	0.993688269933714
>100	0.017660387342642723	1.3964142952936776
>500	0.002943397890439723	1.0549506126880634
>1k	0.0035974863105374395	3.3269753512339864
>5k	3.270442100488581E-4	1.2991401873915973
>10k+	3.270442100488581E-4	1.7690114313129095
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12330	1.753235950295121	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	9055	1.2875548686068388	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCG	3206	0.4558697856160712	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGC	3107	0.44179270864289866	No Hit
GAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	3065	0.4358206153815528	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2316	0.3293182855542173	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	2263	0.3217820726291856	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	1846	0.26248771810582266	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	1753	0.2492637973128424	No Hit
CGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	1622	0.23063655404531114	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	1477	0.21001861302399785	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.19736346301781246	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	1146	0.16295283041672415	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	979	0.1392066500680392	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTC	976	0.13878007197794306	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	973	0.13835349388784693	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	931	0.13238140062650103	TruSeq Adapter, Index 19 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	917	0.13039070287271906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	760	0.10806644949102125	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTC	730	0.10380066859005989	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8438539339742435E-4	0.0	0.0	0.13764253040435337	0.0
2	2.8438539339742435E-4	0.0	0.0	0.6857953761778888	0.0
3	2.8438539339742435E-4	0.0	0.0	1.1046950606522947	0.0
4	2.8438539339742435E-4	0.0	0.0	1.960268516688446	0.0
5	2.8438539339742435E-4	0.0	0.0	4.237768939711718	0.0
6	2.8438539339742435E-4	0.0	0.0	6.0454646928424465	0.0
7	2.8438539339742435E-4	0.0	0.0	7.276995638949992	0.0
8	2.8438539339742435E-4	0.0	0.0	8.794902676208745	0.0
9	2.8438539339742435E-4	0.0	0.0	9.603836927727718	0.0
10	2.8438539339742435E-4	0.0	0.0	11.083920707664612	0.0
11	2.8438539339742435E-4	0.0	0.0	12.067609783426304	0.0
12	2.8438539339742435E-4	0.0	0.0	13.407775949811667	0.0
13	2.8438539339742435E-4	0.0	0.0	13.940145406251643	0.0
14	2.8438539339742435E-4	0.0	0.0	14.261074322700637	0.0
15	2.8438539339742435E-4	0.0	0.0	14.600914867810559	0.0
16	2.8438539339742435E-4	0.0	0.0	15.043845118027049	0.0
17	2.8438539339742435E-4	0.0	0.0	15.552184008724943	0.0
18	2.8438539339742435E-4	0.0	0.0	16.102043166858863	0.0
19	2.8438539339742435E-4	0.0	0.0	16.55634883281125	0.0
20	2.8438539339742435E-4	0.0	0.0	16.953919612780847	0.0
21	2.8438539339742435E-4	0.0	0.0	17.367273782084006	0.0
22	2.8438539339742435E-4	0.0	0.0	17.84390370141809	0.0
23	2.8438539339742435E-4	0.0	0.0	18.293517008379414	0.0
24	4.265780900961365E-4	0.0	0.0	18.678006060252734	0.0
25	4.265780900961365E-4	0.0	0.0	19.023534313230606	0.0
26	4.265780900961365E-4	0.0	0.0	19.374750274076423	0.0
27	4.265780900961365E-4	0.0	0.0	19.77018816359554	0.0
28	4.265780900961365E-4	0.0	0.0	20.133917081750848	0.0
29	4.265780900961365E-4	0.0	0.0	20.529212778573267	0.0
30	4.265780900961365E-4	0.0	0.0	20.99361412599126	0.0
31	4.265780900961365E-4	0.0	0.0	21.3980101554024	0.0
32	4.265780900961365E-4	0.0	0.0	21.809942397738567	0.0
33	4.265780900961365E-4	0.0	0.0	22.207655370404865	0.0
34	4.265780900961365E-4	0.0	0.0	22.615037446446674	0.0
35	4.265780900961365E-4	0.0	0.0	23.029386964626724	0.0
36	4.265780900961365E-4	0.0	0.0	23.432076681677476	0.0
37	4.265780900961365E-4	0.0	0.0	23.841876033563164	0.0
38	4.265780900961365E-4	0.0	0.0	24.266747811298917	0.0
39	4.265780900961365E-4	0.0	0.0	24.77309600424303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGG	35	1.2110286E-7	45.000004	2
TAGCACG	20	7.031416E-4	45.0	1
CGTAGTA	20	7.031416E-4	45.0	10
GCGTAAG	40	6.8084773E-9	45.0	1
CGCCCGT	20	7.031416E-4	45.0	38
CGTTTTT	7255	0.0	44.007584	1
CGTTATT	815	0.0	43.343555	1
TGCGAAG	70	0.0	41.785717	1
ACGGGTC	50	1.0804797E-9	40.5	5
CCCGTCG	50	1.0804797E-9	40.5	40
CGTTTCT	235	0.0	39.255318	1
TGGGCGA	315	0.0	38.57143	6
CCGATGA	455	0.0	38.076923	18
CCGTCGA	65	9.094947E-12	38.07692	41
CGCGACC	60	1.546141E-10	37.500004	10
CGTTACT	30	1.1394713E-4	37.500004	1
TTAACGG	60	1.546141E-10	37.500004	2
CGATGAT	30	1.1394713E-4	37.500004	10
GCCCGTC	30	1.1394713E-4	37.500004	39
GTTTTTT	8605	0.0	37.391052	2
>>END_MODULE