FastQCFastQC Report
Sat 14 Jan 2017
SRR2935837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863711
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC179522.0784730077537508No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC141121.6338798510149806No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG140231.6235754783718166No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60450.6998868834598609No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC47680.5520365029506398RNA PCR Primer, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT41010.4748115978608585RNA PCR Primer, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT32140.3721152098329186No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC23370.27057661648398595No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT22290.25807243395070806No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA20720.23989505749029477No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT19310.22357015251629306No Hit
GAATATGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC18160.2102555137077101No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG16370.18953098895348097No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC16330.18906787108187809No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC14010.16220703452891072RNA PCR Primer, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG13450.15572338432647032RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT13040.15097642614254073RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT12350.14298764285739096No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC10440.12087376448835316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC207.0323114E-445.034
CGATGAA18650.043.1903519
TACGGCT18050.042.756237
CCGATGA18450.042.43902618
TAATACG5550.042.1621674
ACGGCTG18450.041.829278
TACGAAT700.041.78571312
AATACGG5550.041.756765
CGTTTTT24300.041.6666641
CGTAAGG1300.041.538462
CCACCGA23900.040.5753115
CTCGAAT2950.040.4237343
GATGAAT20650.040.42372520
ACCACCG25350.040.02958714
TTACGGG1800.040.03
ACCGATG19450.039.9100317
GATACCT17100.039.6052635
ATGAATG20750.039.4698821
CGTTATT2800.039.3751
ATTGCGG800.039.3752
Produced by FastQC (version 0.11.5)