##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935837.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863711
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936690629157205	33.0	31.0	34.0	30.0	34.0
2	32.0546444354651	33.0	31.0	34.0	30.0	34.0
3	32.00588159696936	33.0	31.0	34.0	30.0	34.0
4	35.686366157198414	37.0	35.0	37.0	33.0	37.0
5	32.00912805324929	37.0	35.0	37.0	0.0	37.0
6	33.76757503377866	37.0	35.0	37.0	17.0	37.0
7	35.36667241704691	37.0	35.0	37.0	32.0	37.0
8	35.535461514325974	37.0	35.0	37.0	33.0	37.0
9	37.47112170621886	39.0	37.0	39.0	35.0	39.0
10	37.17574512771054	39.0	37.0	39.0	34.0	39.0
11	37.120629469811085	39.0	37.0	39.0	34.0	39.0
12	37.10111947167513	39.0	37.0	39.0	34.0	39.0
13	37.01410425477967	39.0	37.0	39.0	33.0	39.0
14	38.20712252130632	40.0	38.0	41.0	33.0	41.0
15	38.25324211454989	40.0	38.0	41.0	33.0	41.0
16	38.34072276490632	40.0	37.0	41.0	34.0	41.0
17	38.199029536500056	40.0	37.0	41.0	33.0	41.0
18	38.130388521160434	40.0	37.0	41.0	33.0	41.0
19	38.02384709700351	40.0	37.0	41.0	33.0	41.0
20	37.844173571947096	40.0	36.0	41.0	33.0	41.0
21	37.97453314824056	40.0	37.0	41.0	33.0	41.0
22	38.028563952525786	40.0	37.0	41.0	33.0	41.0
23	37.942440237533155	40.0	37.0	41.0	33.0	41.0
24	37.945158739439464	40.0	36.0	41.0	33.0	41.0
25	37.84208375255149	40.0	36.0	41.0	33.0	41.0
26	37.78922232089206	40.0	36.0	41.0	33.0	41.0
27	37.69963564201451	40.0	36.0	41.0	33.0	41.0
28	37.62197309053607	40.0	36.0	41.0	33.0	41.0
29	37.62809782438802	40.0	36.0	41.0	33.0	41.0
30	37.4707778411992	40.0	36.0	41.0	33.0	41.0
31	37.418677080643874	40.0	36.0	41.0	32.0	41.0
32	37.211414466181395	40.0	36.0	41.0	32.0	41.0
33	37.08546029864156	40.0	36.0	41.0	31.0	41.0
34	36.81038912321367	40.0	35.0	41.0	31.0	41.0
35	36.81037986085624	40.0	35.0	41.0	31.0	41.0
36	36.74496909267104	40.0	35.0	41.0	31.0	41.0
37	36.74245320483356	40.0	35.0	41.0	30.0	41.0
38	36.6014662311815	40.0	35.0	41.0	30.0	41.0
39	36.41026222891685	40.0	35.0	41.0	30.0	41.0
40	36.42141063388101	39.0	35.0	41.0	30.0	41.0
41	36.19686098706627	39.0	35.0	41.0	28.0	41.0
42	36.25681043775059	39.0	35.0	41.0	30.0	41.0
43	36.22846530841914	39.0	35.0	41.0	30.0	41.0
44	36.26852731990214	39.0	35.0	41.0	30.0	41.0
45	36.312713396031775	39.0	35.0	41.0	30.0	41.0
46	36.173186401469934	39.0	35.0	41.0	30.0	41.0
47	35.97406076801152	39.0	35.0	41.0	29.0	41.0
48	35.99643515018334	39.0	35.0	41.0	29.0	41.0
49	36.05374482899951	39.0	35.0	41.0	29.0	41.0
50	35.901953315402956	39.0	35.0	41.0	28.0	41.0
51	34.885556627158856	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	16.0
9	30.0
10	32.0
11	27.0
12	12.0
13	17.0
14	22.0
15	31.0
16	54.0
17	92.0
18	212.0
19	548.0
20	1304.0
21	2753.0
22	4192.0
23	5892.0
24	7135.0
25	8308.0
26	9172.0
27	9297.0
28	9613.0
29	10536.0
30	12623.0
31	16670.0
32	22324.0
33	30964.0
34	48500.0
35	60454.0
36	64231.0
37	98119.0
38	163178.0
39	277169.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.059825566653664	18.235381973831526	20.970093005646564	12.73469945386825
2	30.440390362053975	32.31578618310986	21.492142626410917	15.751680828425249
3	27.57068047066669	32.13123371127611	25.64804662670731	14.650039191349883
4	25.33115822306304	21.938009357296593	35.68473714008505	17.046095279555313
5	27.503759938220075	33.14268314285681	21.942293197608922	17.411263721314192
6	23.02355764833376	37.75695805657217	25.362650238332034	13.856834056762041
7	72.5092073621848	4.698909704750779	18.375243571055595	4.41663936200882
8	72.05060488983005	11.4909964096787	11.480923595971337	4.977475104519915
9	66.56508948016176	6.125312749287667	12.050095460171285	15.259502310379283
10	39.53891984703217	25.34424130293582	20.07523349824189	15.041605351790125
11	28.713308039378916	22.395685593908148	28.61315879964479	20.277847567068154
12	23.180670386275036	19.636545094366056	32.23149873047814	24.951285788880774
13	24.77483787980007	21.111228177017544	36.07954512562651	18.03438881755587
14	19.631335018310523	28.884082754532475	30.07707439178151	21.40750783537549
15	16.832250602342683	23.961487117797503	37.33285786565182	21.873404414207993
16	20.64336334723073	25.245597196284404	28.705666594497465	25.405372861987402
17	20.62449129396291	26.553094727287252	30.67715937391095	22.145254604838886
18	19.963969429589294	24.704791301720135	30.750216218156307	24.581023050534263
19	19.723263915823697	25.996542825088486	28.48788541537621	25.79230784371161
20	22.249340346481635	26.695850811208842	32.26299074574713	18.79181809656239
21	21.184863918602403	29.619282375702056	30.517036369804252	18.67881733589129
22	19.79921524676657	21.791548330402183	33.391956337247066	25.017280085584183
23	20.804875704952234	26.599406514447544	31.76675994632464	20.828957834275585
24	21.43193730310254	25.51084795724496	29.12710385765609	23.93011088199641
25	19.748619619293954	31.80415671445657	27.228667922487958	21.218555743761513
26	19.332623991126663	24.14546069229175	31.364889413241237	25.15702590334035
27	23.157398713226993	25.321895865630985	28.099213741633488	23.421491679508538
28	17.306251743928236	26.590259936483385	32.00572876807173	24.097759551516653
29	19.746304029935942	22.595173617101093	32.92640709681827	24.732115256144706
30	19.089371329067244	27.16730480450058	29.44665518906208	24.296668677370093
31	20.67740251079354	26.79298978477755	26.488952902070253	26.04065480235866
32	22.700880271294448	26.537580278588557	26.112669631392908	24.648869818724087
33	20.532099278578137	23.879631033991693	25.103188450766517	30.485081236663653
34	20.03100574150381	26.733363358808678	28.393062031165517	24.842568868521994
35	19.629019428952507	26.609710887090703	26.230648909183746	27.530620774773045
36	18.808027222068493	28.296270396000512	27.565470394611157	25.330231987319834
37	17.571502504888787	25.9193179199987	28.585487506816516	27.923692068295992
38	18.214541669609392	27.59603617413695	24.89478540854522	29.294636747708434
39	19.96767437256212	26.275802901665024	26.81880860611941	26.93771411965345
40	19.602621710271144	25.327221721154412	28.84807534001535	26.22208122855909
41	18.036125509574383	28.12051716372722	26.165580848223534	27.677776478474858
42	18.846234446475734	26.09310290131769	30.772908993864846	24.28775365834174
43	21.39616144752122	23.76558825810948	26.768097199178893	28.070153095190403
44	20.610366198879024	23.057712591364474	29.23281051184945	27.099110697907054
45	19.419342812584304	22.723225708599287	28.121443399470426	29.735988079345987
46	24.502640350765475	24.908678944693303	26.93563008923124	23.653050615309983
47	17.571270945952985	24.709885598307768	33.476475348814596	24.242368106924655
48	19.596138060068704	26.32385138084382	27.679976288364973	26.4000342707225
49	19.55549946683555	22.735266773260964	33.1559977816654	24.553235978238092
50	20.297761635547076	22.512159738616273	30.736091123072416	26.453987502764235
51	19.201677412930945	22.65294757158355	28.353002335271864	29.792372680213635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	272.0
1	569.5
2	867.0
3	5482.0
4	10097.0
5	6424.5
6	2752.0
7	2640.0
8	2528.0
9	2535.0
10	2542.0
11	2448.5
12	2355.0
13	2270.5
14	2186.0
15	2137.5
16	2089.0
17	1984.5
18	1880.0
19	1921.0
20	1962.0
21	2074.5
22	2187.0
23	2563.5
24	2940.0
25	3577.0
26	5265.0
27	6316.0
28	7487.0
29	8658.0
30	10117.5
31	11577.0
32	13487.0
33	15397.0
34	18039.5
35	20682.0
36	22467.0
37	24252.0
38	27311.0
39	30370.0
40	33160.5
41	35951.0
42	40327.5
43	44704.0
44	51785.5
45	58867.0
46	94336.5
47	129806.0
48	107374.5
49	84943.0
50	84316.0
51	83689.0
52	72934.5
53	62180.0
54	55955.0
55	49730.0
56	46180.0
57	42630.0
58	38151.0
59	33672.0
60	29240.5
61	24809.0
62	22225.5
63	19642.0
64	16648.0
65	13654.0
66	11353.5
67	9053.0
68	7475.5
69	5898.0
70	4770.0
71	3642.0
72	2974.5
73	2307.0
74	1735.5
75	864.0
76	564.0
77	467.5
78	371.0
79	277.0
80	183.0
81	133.5
82	84.0
83	54.5
84	25.0
85	18.5
86	12.0
87	7.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	863711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.71876709157494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65872707329751	23.773039682815323
2	10.05574257877827	6.580229987357589
3	3.8527648227186964	3.7817314467943812
4	2.068104164689143	2.7066327394232084
5	1.3013813575904043	2.128979676815902
6	0.9715268510285419	1.9072296457208453
7	0.7856871875162748	1.7994701266625697
8	0.6929667440175956	1.8138413999774994
9	0.6491915471105567	1.9116672324956556
>10	6.871571153580179	39.96479739505391
>50	0.05137791059004712	1.1410964394275966
>100	0.030539313789805744	1.8644058709528293
>500	0.0035928604458593657	0.7289521315118697
>1k	0.005389290668789048	3.7696029674554308
>5k	3.5928604458593656E-4	0.7106137131090553
>10k+	0.0010778581337578096	5.417709544426309
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	17952	2.0784730077537508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	14112	1.6338798510149806	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	14023	1.6235754783718166	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6045	0.6998868834598609	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4768	0.5520365029506398	RNA PCR Primer, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	4101	0.4748115978608585	RNA PCR Primer, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	3214	0.3721152098329186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	2337	0.27057661648398595	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	2229	0.25807243395070806	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	2072	0.23989505749029477	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1931	0.22357015251629306	No Hit
GAATATGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	1816	0.2102555137077101	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	1637	0.18953098895348097	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	1633	0.18906787108187809	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1401	0.16220703452891072	RNA PCR Primer, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	1345	0.15572338432647032	RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1304	0.15097642614254073	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1235	0.14298764285739096	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	1044	0.12087376448835316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4733840370216424E-4	0.0	0.0	0.15746007634498113	0.0
2	3.4733840370216424E-4	0.0	0.0	1.0013766178733396	0.0
3	3.4733840370216424E-4	0.0	0.0	1.3298429683076862	0.0
4	3.4733840370216424E-4	0.0	0.0	2.1430779508423536	0.0
5	3.4733840370216424E-4	0.0	0.0	5.241683850269361	0.0
6	3.4733840370216424E-4	0.0	0.0	6.361965981676741	0.0
7	3.4733840370216424E-4	0.0	0.0	7.463144500880503	0.0
8	3.4733840370216424E-4	0.0	0.0	8.676976442351666	0.0
9	3.4733840370216424E-4	0.0	0.0	9.088340891802929	0.0
10	3.4733840370216424E-4	0.0	0.0	11.659108197070548	0.0
11	4.631178716028857E-4	0.0	0.0	12.751719035649655	0.0
12	4.631178716028857E-4	0.0	0.0	15.50958596104484	0.0
13	5.788973395036071E-4	0.0	0.0	15.989607634961231	0.0
14	5.788973395036071E-4	0.0	0.0	16.265625886436553	0.0
15	5.788973395036071E-4	0.0	0.0	16.818472845662495	0.0
16	5.788973395036071E-4	0.0	0.0	17.431062010325213	0.0
17	5.788973395036071E-4	0.0	0.0	18.100267334791383	0.0
18	5.788973395036071E-4	0.0	0.0	18.760210301825495	0.0
19	5.788973395036071E-4	0.0	0.0	19.5636040295886	0.0
20	5.788973395036071E-4	0.0	0.0	20.037836730109955	0.0
21	5.788973395036071E-4	0.0	0.0	20.562549278636027	0.0
22	5.788973395036071E-4	0.0	0.0	21.228281219065174	0.0
23	5.788973395036071E-4	0.0	0.0	21.803126277192256	0.0
24	5.788973395036071E-4	0.0	0.0	22.268328179217352	0.0
25	5.788973395036071E-4	0.0	0.0	22.682355556430334	0.0
26	5.788973395036071E-4	0.0	0.0	23.080868484944617	0.0
27	5.788973395036071E-4	0.0	0.0	23.557416774823988	0.0
28	5.788973395036071E-4	0.0	0.0	23.966234075981433	0.0
29	5.788973395036071E-4	0.0	0.0	24.395197004553605	0.0
30	5.788973395036071E-4	0.0	0.0	24.93496088390677	0.0
31	6.946768074043285E-4	0.0	0.0	25.393331797325725	0.0
32	6.946768074043285E-4	0.0	0.0	25.82078959281519	0.0
33	6.946768074043285E-4	0.0	0.0	26.253457464360185	0.0
34	6.946768074043285E-4	0.0	0.0	26.702218681943382	0.0
35	8.1045627530505E-4	0.0	0.0	27.176335602996836	0.0
36	8.1045627530505E-4	0.0	0.0	27.60205670646779	0.0
37	9.262357432057714E-4	0.0	0.0	28.030556517168357	0.0
38	9.262357432057714E-4	0.0	0.0	28.466929331686178	0.0
39	9.262357432057714E-4	0.0	0.0	29.026723058986164	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGC	20	7.0323114E-4	45.0	34
CGATGAA	1865	0.0	43.19035	19
TACGGCT	1805	0.0	42.75623	7
CCGATGA	1845	0.0	42.439026	18
TAATACG	555	0.0	42.162167	4
ACGGCTG	1845	0.0	41.82927	8
TACGAAT	70	0.0	41.785713	12
AATACGG	555	0.0	41.75676	5
CGTTTTT	2430	0.0	41.666664	1
CGTAAGG	130	0.0	41.53846	2
CCACCGA	2390	0.0	40.57531	15
CTCGAAT	295	0.0	40.42373	43
GATGAAT	2065	0.0	40.423725	20
ACCACCG	2535	0.0	40.029587	14
TTACGGG	180	0.0	40.0	3
ACCGATG	1945	0.0	39.91003	17
GATACCT	1710	0.0	39.605263	5
ATGAATG	2075	0.0	39.46988	21
CGTTATT	280	0.0	39.375	1
ATTGCGG	80	0.0	39.375	2
>>END_MODULE